Binary fission in Trichoplax is orthogonal to the subsequent division plane.

Asexual reproduction in Trichoplax occurs mainly by binary fission and occasionally by the budding of epithelial spheres called "swarmers". The process that leads to binary fission and the mechanisms involved in this segregation are practically unknown. Trichoplax lacks a defined shape, presenting a constantly changing outline due to its continuous movements and body contractions. For this reason, and due to the absence of anatomical references, it has been classified as an asymmetric organism. Here, we report that a transient wound is formed in the marginal epithelium of the two new individuals produced by binary fission. By tracking the location of this epithelial wound, we can determine that successive dichotomous divisions are orthogonal to the previous division. We also found that LiCl paralyzes the cilia beating movement and body contractions and causes the placozoans to become circular in shape. This effect, as well as a stereotypic body folding behavior observed in detached placozoans and cell labeling experiments of the upper epithelium, indicate a cylindrical body symmetry for Placozoa.

The Evolutionary History of The Orexin/Allatotropin GPCR Family: from Placozoa and Cnidaria to Vertebrata.

Peptidic messengers constitute a highly diversified group of intercellular messengers widely distributed in nature that regulate a great number of physiological processes in Metazoa. Being crucial for life, it seem that they have appeared in the ancestral group from which Metazoa evolved, and were highly conserved along the evolutionary process. Peptides act mainly through G-protein coupled receptors (GPCRs), a family of transmembrane molecules. GPCRs are also widely distributed in nature being present in metazoan, but also in Choanoflagellata and Fungi. Among GPCRs, the Allatotropin/Orexin (AT/Ox) family is particularly characterized by the presence of the DRW motif in the second intracellular loop (IC Loop 2), and seems to be present in Cnidaria, Placozoa and in Bilateria, suggesting that it was present in the common ancestor of Metazoa. Looking for the evolutionary history of this GPCRs we searched for corresponding sequences in public databases. Our results suggest that AT/Ox receptors were highly conserved along evolutionary process, and that they are characterized by the presence of the E/DRWYAI motif at the IC Loop 2. Phylogenetic analyses show that AT/Ox family of receptors reflects evolutionary relationships that agree with current phylogenetic understanding in Actinopterygii and Sauropsida, including also the largely discussed position of Testudines.