Bone Regenerative Effect of Injectable Hypoxia Preconditioned Serum-Fibrin (HPS-F) in an Ex Vivo Bone Defect Model.

Biofunctionalized hydrogels are widely used in tissue engineering for bone repair. This study examines the bone regenerative effect of the blood-derived growth factor preparation of Hypoxia Preconditioned Serum (HPS) and its fibrin-hydrogel formulation (HPS-F) on drilled defects in embryonic day 19 chick femurs. Measurements of bone-related growth factors in HPS reveal significant elevations of Osteopontin, Osteoprotegerin, and soluble-RANKL compared with normal serum (NS) but no detection of BMP-2/7 or Osteocalcin. Growth factor releases from HPS-F are measurable for at least 7 days. Culturing drilled femurs organotypically on a liquid/gas interface with HPS media supplementation for 10 days demonstrates a 34.6% increase in bone volume and a 52.02% increase in bone mineral density (BMD) within the defect area, which are significantly higher than NS and a basal-media-control, as determined by microcomputed tomography. HPS-F-injected femur defects implanted on a chorioallantoic membrane (CAM) for 7 days exhibit an increase in bone mass of 123.5% and an increase in BMD of 215.2%, which are significantly higher than normal-serum-fibrin (NS-F) and no treatment. Histology reveals calcification, proteoglycan, and collagen fiber deposition in the defect area of HPS-F-treated femurs. Therefore, HPS-F may offer a promising and accessible therapeutic approach to accelerating bone regeneration by a single injection into the bone defect site.

In Vitro Comparison of Lymphangiogenic Potential of Hypoxia Preconditioned Serum (HPS) and Platelet-Rich Plasma (PRP).

Strategies for therapeutic lymphangiogenesis are gradually directed toward the use of growth factor preparations. In particular, blood-derived growth factor products, including Hypoxia Preconditioned Serum (HPS) and Platelet-rich Plasma (PRP), are both clinically employed for accelerating tissue repair and have received considerable attention in the field of regenerative medicine research. In this study, a comparative analysis of HPS and PRP was conducted to explore their lymphangiogenic potential. We found higher pro-lymphangiogenic growth factor concentrations of VEGF-C, PDGF-BB, and bFGF in HPS in comparison to normal serum (NS) and PRP. The proliferation and migration of lymphatic endothelial cells (LECs) were promoted considerably with both HPS and PRP, but the strongest effect was achieved with HPS-40% dilution. Tube formation of LECs showed the highest number of tubes, branching points, greater tube length, and cell-covered area with HPS-10%. Finally, the effects were double-validated using an ex vivo lymphatic ring assay, in which the highest number of sprouts and the greatest sprout length were achieved with HPS-10%. Our findings demonstrate the superior lymphangiogenic potential of a new generation blood-derived secretome obtained by hypoxic preconditioning of peripheral blood cells-a method that offers a novel alternative to PRP.

A Narrative Review of the Usage of Creative Solutions to Enhance Disabled Patients' Quality of Life and Wellbeing by Occupational Therapists.

Introduction: Occupational therapists play a crucial role in the rehabilitation process, as they maximise patients' independence and participation in meaningful occupations by adopting creative solutions in their treatment plan. Recognizing the importance of creative work and incorporating it into daily activities can be a powerful technique for enhancing quality of life. As a result, OTs must comprehend the significance of using creativity on a daily basis with patients. Creativity has been implicit in occupational therapists' values and practices since the occupational therapy field's early days. In particular, OTs use meaningful activities to promote health, wellbeing, and independence, which have traditionally had a strong connection with creativity. Primarily, the OT uses practical exercise to create wellbeing and autonomy, which have had a healthy linkage with imagination. The OT has used creative exercise as a treatment technique since the beginning of the profession. Method: In this paper, a Narrative Review Checklist was used to carry out specific checks of manuscripts' structures and a careful selection of the articles which are included in the manuscript. In addition, articles referenced in review articles or guidelines were reviewed for inclusion. Findings. This review has shown that the primary motivation for implementing creative activities was to improve the patient performance, wellbeing, and self-esteem. The goal of implementing creative activities was to assist the client in self-expression as well as experience joy and desire. As a result, the necessity to investigate the influence of creative solutions in treatment from an OT standpoint has been identified. Conclusion: Future studies should focus on how it is important to consider the patient's intrinsic and extrinsic motivations when designing creative solutions, as well as how the disability affects their daily activities, occupations, and adherence to a treatment plan.

Hypoxia Preconditioned Serum (HPS) Promotes Osteoblast Proliferation, Migration and Matrix Deposition.

Interest in discovering new methods of employing natural growth factor preparations to promote bone fracture healing is becoming increasingly popular in the field of regenerative medicine. In this study, we were able to demonstrate the osteogenic potential of hypoxia preconditioned serum (HPS) on human osteoblasts in vitro. Human osteoblasts were stimulated with two HPS concentrations (10% and 40%) and subsequently analyzed at time points of days 2 and 4. In comparison to controls, a time- and dose-dependent (up to 14.2× higher) proliferation of osteoblasts was observed after 4 days of HPS-40% stimulation with lower lactate dehydrogenase (LDH)-levels detected than controls, indicating the absence of cytotoxic/stress effects of HPS on human osteoblasts. With regards to cell migration, it was found to be significantly faster with HPS-10% application after 72 h in comparison to controls. Further osteogenic response to HPS treatment was evaluated by employing culture supernatant analysis, which exhibited significant upregulation of OPG (Osteoprotegerin) with higher dosage (HPS-10% vs. HPS-40%) and longer duration (2 d vs. 4 d) of HPS stimulation. There was no detection of anti-osteogenic sRANKL (soluble Receptor Activator of NF-κB Ligand) after 4 days of HPS stimulation. In addition, ALP (alkaline phosphatase)-enzyme activity, was found to be upregulated, dose-dependently, after 4 days of HPS-40% application. When assessing ossification through Alizarin-Red staining, HPS dose-dependently achieved greater (up to 2.8× higher) extracellular deposition of calcium-phosphate with HPS-40% in comparison to controls. These findings indicate that HPS holds the potential to accelerate bone regeneration by osteogenic promotion of human osteoblasts.

Linguistic masking release in young and older adults with age-appropriate hearing status.

Past studies of speech-on-speech masking in young adults (YA) indicate that the intelligibility of masked speech can improve if the target and masker speech are in different languages. Current work investigated whether a linguistic masking release is obtained in older adults (OA) with age-typical hearing abilities. Participants listened to English sentences in the presence of two-talker Spanish or English maskers. A similar masking release with Spanish-language maskers was obtained for OA and YA listeners, despite greater accuracy by YA listeners. In speech-on-speech masking, older listeners can thus improve speech intelligibility by utilizing nonenergetic linguistic differences between the target and masker speech.