Identification of microorganisms in irreversible pulpitis and primary endodontic infections with respect to clinical and radiographic findings.

OBJECTIVES: The aim of this study was to evaluate the composition of microbiota of irreversible pulpitis and primary endodontic infections with respect to clinical and radiographic findings by performing cultures and 16s rDNA sequencing in Iranian patients. MATERIAL AND METHODS: In this prospective cross-sectional study, samples were collected from 41 root canals for 4 main groups of patients. Bacterial identification was performed by the polymerase chain reaction (PCR) and 16s rDNA sequencing of aerobic and anaerobic cultivable colonies taken from patients' culture plates. Additionally, the presence of 13 bacterial species and 3 nonbacterial species was also explored using PCR and species-specific primers. RESULTS: Sixteen microbial species, 1 fungus (Candida albicans), and 1 virus (Herpes simplex virus) were discovered and isolated. Species with the highest prevalence were Dialister invisus (68.3%), Porphyromonas gingivalis (58.8%), Streptococcus salivarius (58.5%), and Treponema denticola (56.1%). Lysinibacillus fusiformis (19.1%) was detected in the root canals for the first time. Candida albicans was seen in 11 cases (26.8%). Herpes simplex virus (HSV) was seen in 4 patients (9.8%). CONCLUSIONS: Our results suggest that Gram-negative anaerobic oral bacteria are the majority of the microbes in primary endodontic infections. Various combinations of bacterial species were related to different clinical and radiographic conditions. Lysinibacillus fusiformis was detected for the first time in primary endodontic infections. CLINICAL RELEVANCE: The results of this investigation might help clinicians choose to identify suspected endodontic pathogens in the etiology of each form of pulpal and periradicular diseases to determine the best therapeutic measures.

Interleukin-2, -16, and -17 gene polymorphisms in Iranian patients with chronic periodontitis.

AIM: Chronic periodontitis (CP) is a multifactorial disease and the most common type of periodontitis mainly caused by microbial plaque. CP can be brought on by, and progresses with, insufficient oral hygiene, and environmental and genetic susceptibilities. The aim of the present study was to investigate the association between interleukin (IL)-2 (T-330G), IL-16 (T-295C), and IL-17 (A-7383G) gene polymorphisms and the susceptibility to CP in an Iranian population. METHODS: Ninety-nine cases diagnosed with CP and 75 matched healthy controls engaged in the present study. 3 cc peripheral blood samples were obtained for DNA isolation. Genotype analysis was performed using restriction fragment length polymorphism polymerase chain reaction. Genotype distribution and allele frequencies within groups were compared using χ2 -test, and logistic regression analysis was used to recognize the independent relation between the disease and the absence or presence of alleles. RESULTS: There was no polymorphism in IL-2 (T-330G) among our patients, and the TT genotype was present in both study groups. Moreover, none of the studied genotypes and alleles of IL-16 (T-295C) and IL-17 (A-7383G) was significantly associated with CP. CONCLUSION: The present study demonstrated no association between IL-2 (T-330G), IL-16 (T-295C), and IL-17 (A-7383G) genotypes and CP in an Iranian population.

Effect of Amperage and Field of View on Detection of Vertical Root Fracture in Teeth with Intracanal Posts.

INTRODUCTION: This study sought to assess the effects of amperage (mA) and field of view (FOV) on intracanal metal post artifacts and the diagnostic parameters for detection of vertical root fracture (VRF) in teeth with intracanal posts. METHODS AND MATERIALS: In this diagnostic study, 80 human single-canal teeth were evaluated by cone-beam computed tomography (CBCT). Nickel chrome cast posts were placed into root canals after root canal therapy and canal preparation. In the test group, fracture was induced by an instron machine while no fracture was induced in the control group. Deterministic and probabilistic sensitivity and specificity values at different exposure settings were statistically analyzed using the one-way ANOVA and pairwise comparisons were performed by Tukey's test. RESULTS: Significant differences were found between the two groups in terms of deterministic (P<0.0001) and probabilistic (P<0.013) sensitivity and deterministic (P<0.037) and probabilistic (P<0.0001) specificity at different FOV and mA combinations. CONCLUSION: A smaller FOV and lower mA should be preferably used for detection of VRFs in teeth with intracanal posts.