Practical Principles of Palliative Care in Veterinary Oncology: Alleviating the Suffering of the Animal, Owner, and Veterinarian.

Background: Palliative care for pets is a developing area that encompasses animals with cancer and other chronic and degenerative diseases. Objectives: To elucidate the guiding principles of palliative care in veterinary oncology. Methods: This article was structured from an extensive literature review and addresses the growing need for improved palliative care in veterinary medicine. Methods of quality of life assessment in animals with cancer, owner education, and the importance of an interdisciplinary team approach are discussed. Results: Palliative care aims to improve the animal's quality of life, alleviating its physical, emotional, and social suffering. Through attentive communication, palliative care also seeks to alleviate owners suffering from the moment of diagnosis to mourning the patient's death. Conclusions: The importance of offering palliative care alongside anticancer techniques and treatments should not be underestimated and should ideally use a multidisciplinary team approach.

Clinical Implications of Immune Checkpoints and the RANK/RANK-L Signaling Pathway in High-Grade Canine Mast Cell Tumors.

Mast cell tumors (MCTs) are the most common malignant cutaneous tumors in dogs, and they present extremely variable biological behavior. The interaction between RANK, RANK-L, and immune checkpoints is frequently detected in the tumor microenvironment, and, together, they participate in every stage of cancer development. Thus, the aim of this study was to characterize the molecular profiles of PD-L1, CTLA-4, RANK/RANK-L signaling pathway, and IFN-γ in primary tumors and lymph node metastases. Formalin-fixed, paraffin-embedded slides of MCTs and metastatic lymph nodes of ten dogs were submitted to immunohistochemical investigations. The results demonstrated that the tumor microenvironment of the high-grade mast cell tumors showed moderate or intense immunolabeling of all proteins, and the lymph node metastases also showed moderate or intense immunolabeling of checkpoint proteins. In addition, MCTs larger than 3 cm were associated with intensified PD-L1 (p = 0.03) in metastatic lymph nodes and RANK-L (p = 0.049) immunoreactivity in the tumor. Furthermore, dogs with a survival time of less than 6 months showed higher PD-L1 immunoreactivity (p = 0.042). In conclusion, high-grade MCT is associated with an immunosuppressive microenvironment that exhibits elevated RANK/RANK-L signaling and enhanced immune checkpoint immunoreactivity, potentially facilitating intratumorally immune escape. These biomarkers show promise as clinical indicators of disease progression and might response to immunotherapy in dogs with high-grade MCTs, thus emphasizing their importance for guiding treatment decisions and improving outcomes.

Topical and intradermal delivery of PpIX precursors for photodynamic therapy with intense pulsed light on porcine skin model.

In order to purposely decrease the time of the photodynamic therapy (PDT) sessions, this study evaluated the effects of PDT using topical and intradermal delivery of two protoporphyrin (PpIX) precursors with intense pulsed light (IPL) as irradiation source. This study was performed on porcine skin model, using an IPL commercial device (Intense Pulse Light, HKS801). IPL effect on different administration methods of two PpIX precursors (ALA and MAL) was investigated: a topical cream application and an intradermal application using a needle-free, high-pressure injection system. Fluorescence investigation showed that PpIX distribution by needle-free injection was more homogeneous than that by cream, suggesting that a shorter drug-light interval in PDT protocols is possible. The damage induced by IPL-PDT assessed by histological analysis mostly shows modifications in collagens fibers and inflammation signals, both expected for PDT. This study suggested an alternative protocol for the PDT treatment, possibility half of the incubation time and with just 3 min of irradiation, making the IPL-PDT, even more, promising for the clinical treatment.

Radical Cystectomy and Cutaneous Ureterostomy in 4 Dogs with Trigonal Transitional Cell Carcinoma: Description of Technique and Case Series.

OBJECTIVE: To describe radical cystectomy followed by cutaneous ureterostomy as a treatment of invasive bladder neoplasia in dogs. STUDY DESIGN: Retrospective study. ANIMALS: Client-owned dogs with transitional cell carcinoma of the bladder trigone (n=4). METHODS: Perioperative complications and long-term outcomes of dogs that underwent cutaneous ureterostomy following radical cystectomy and lymphadenectomy for transitional cell carcinoma of the urinary bladder trigone were reviewed. Both ureters were transected and anastomosed to the ventral abdominal skin. Polyvinyl chloride catheters were placed in the ureteral stomas and maintained for 5 days. After catheter removal, dogs were managed with an absorbent diaper over the stomas. Long-term outcome and survival were documented by follow-up visits or phone contact. RESULTS: Median age at the time of surgery was 10.3 years (range, 8-12). Average procedural time was ∼4.7 hours (range, 3.8-6.1). Minor complications occurred in all dogs, including bleeding and edema of the ureterostomy site during the first 2-3 days after surgery. One dog developed urine scald that resolved with improved stoma care and hygiene. Median survival time after surgery was 278.6 days (range, 47-498). Distant metastases were documented in 2 dogs at 47 days (bone) and 369 days (lung) after surgery. CONCLUSION: Radical cystectomy with cutaneous ureterostomy is a viable salvage procedure for urinary diversion after cystectomy in dogs with invasive bladder neoplasia. Postoperative management and quality of life were considered acceptable by most owners. Future studies are warranted to evaluate survival time in a larger number of animals.

Evaluation of Operating Room Environment Contamination and Efficacy of 2% Chlorhexidine for Surgical Hand Scrubbing before and after Gowning and Gloving

Background: Human skin is colonized by various species of microorganisms, which makes them difficult to eliminate even with the use of antimicrobial drugs. Real efficacy of the antimicrobial product combined with incorrect administration of antibiotics, in addition to potential environmental contamination, are critical points for the establishment of postoperative infection or absence of it. The main objective of the present study was to evaluate the efficacy of surgical antisepsis using a 2% chlorhexidine brush during a surgery, detect operating room environmental contamination, and verify the existence of bacterial resistance to the antibiotics most commonly used in Veterinary Medicine.Materials, Methods & Results: In ten cases of the surgical routine, samples were collected concurrently at specific time points throughout 2 h of surgical procedure. To evaluate the efficacy of 2% chlorhexidine brush on hand scrubbing of a mock surgeon, swabs were used to sample the surface of the hands before and after gowning and gloving. Samples or their dilutions were inoculated onto blood agar and MacConkey agar plates. Contamination of the operating room was evaluated using BHI agar plates distributed through the room. After the incubation period, counts and biochemical tests were performed, and an antimicrobial disc susceptibility test was performed using antibiotics most commonly us

Evaluation of Operating Room Environment Contamination and Efficacy of 2% Chlorhexidine for Surgical Hand Scrubbing before and after Gowning and Gloving

Background: Human skin is colonized by various species of microorganisms, which makes them difficult to eliminate even with the use of antimicrobial drugs. Real efficacy of the antimicrobial product combined with incorrect administration of antibiotics, in addition to potential environmental contamination, are critical points for the establishment of postoperative infection or absence of it. The main objective of the present study was to evaluate the efficacy of surgical antisepsis using a 2% chlorhexidine brush during a surgery, detect operating room environmental contamination, and verify the existence of bacterial resistance to the antibiotics most commonly used in Veterinary Medicine.Materials, Methods & Results: In ten cases of the surgical routine, samples were collected concurrently at specific time points throughout 2 h of surgical procedure. To evaluate the efficacy of 2% chlorhexidine brush on hand scrubbing of a mock surgeon, swabs were used to sample the surface of the hands before and after gowning and gloving. Samples or their dilutions were inoculated onto blood agar and MacConkey agar plates. Contamination of the operating room was evaluated using BHI agar plates distributed through the room. After the incubation period, counts and biochemical tests were performed, and an antimicrobial disc susceptibility test was performed using antibiotics most commonly us

Evaluation of Operating Room Environment Contamination and Efficacy of 2% Chlorhexidine for Surgical Hand Scrubbing before and after Gowning and Gloving

Background: Human skin is colonized by various species of microorganisms, which makes them difficult to eliminate even with the use of antimicrobial drugs. Real efficacy of the antimicrobial product combined with incorrect administration of antibiotics, in addition to potential environmental contamination, are critical points for the establishment of postoperative infection or absence of it. The main objective of the present study was to evaluate the efficacy of surgical antisepsis using a 2% chlorhexidine brush during a surgery, detect operating room environmental contamination, and verify the existence of bacterial resistance to the antibiotics most commonly used in Veterinary Medicine.Materials, Methods & Results: In ten cases of the surgical routine, samples were collected concurrently at specific time points throughout 2 h of surgical procedure. To evaluate the efficacy of 2% chlorhexidine brush on hand scrubbing of a mock surgeon, swabs were used to sample the surface of the hands before and after gowning and gloving. Samples or their dilutions were inoculated onto blood agar and MacConkey agar plates. Contamination of the operating room was evaluated using BHI agar plates distributed through the room. After the incubation period, counts and biochemical tests were performed, and an antimicrobial disc susceptibility test was performed using antibiotics most commonly us

Evaluation of Operating Room Environment Contamination and Efficacy of 2% Chlorhexidine for Surgical Hand Scrubbing before and after Gowning and Gloving

Background: Human skin is colonized by various species of microorganisms, which makes them difficult to eliminate even with the use of antimicrobial drugs. Real efficacy of the antimicrobial product combined with incorrect administration of antibiotics, in addition to potential environmental contamination, are critical points for the establishment of postoperative infection or absence of it. The main objective of the present study was to evaluate the efficacy of surgical antisepsis using a 2% chlorhexidine brush during a surgery, detect operating room environmental contamination, and verify the existence of bacterial resistance to the antibiotics most commonly used in Veterinary Medicine.Materials, Methods & Results: In ten cases of the surgical routine, samples were collected concurrently at specific time points throughout 2 h of surgical procedure. To evaluate the efficacy of 2% chlorhexidine brush on hand scrubbing of a mock surgeon, swabs were used to sample the surface of the hands before and after gowning and gloving. Samples or their dilutions were inoculated onto blood agar and MacConkey agar plates. Contamination of the operating room was evaluated using BHI agar plates distributed through the room. After the incubation period, counts and biochemical tests were performed, and an antimicrobial disc susceptibility test was performed using antibiotics most commonly us

Evaluation of Operating Room Environment Contamination and Efficacy of 2% Chlorhexidine for Surgical Hand Scrubbing before and after Gowning and Gloving

Background: Human skin is colonized by various species of microorganisms, which makes them difficult to eliminate even with the use of antimicrobial drugs. Real efficacy of the antimicrobial product combined with incorrect administration of antibiotics, in addition to potential environmental contamination, are critical points for the establishment of postoperative infection or absence of it. The main objective of the present study was to evaluate the efficacy of surgical antisepsis using a 2% chlorhexidine brush during a surgery, detect operating room environmental contamination, and verify the existence of bacterial resistance to the antibiotics most commonly used in Veterinary Medicine.Materials, Methods & Results: In ten cases of the surgical routine, samples were collected concurrently at specific time points throughout 2 h of surgical procedure. To evaluate the efficacy of 2% chlorhexidine brush on hand scrubbing of a mock surgeon, swabs were used to sample the surface of the hands before and after gowning and gloving. Samples or their dilutions were inoculated onto blood agar and MacConkey agar plates. Contamination of the operating room was evaluated using BHI agar plates distributed through the room. After the incubation period, counts and biochemical tests were performed, and an antimicrobial disc susceptibility test was performed using antibiotics most commonly us

Evaluation of Operating Room Environment Contamination and Efficacy of 2% Chlorhexidine for Surgical Hand Scrubbing before and after Gowning and Gloving

Background: Human skin is colonized by various species of microorganisms, which makes them difficult to eliminate even with the use of antimicrobial drugs. Real efficacy of the antimicrobial product combined with incorrect administration of antibiotics, in addition to potential environmental contamination, are critical points for the establishment of postoperative infection or absence of it. The main objective of the present study was to evaluate the efficacy of surgical antisepsis using a 2% chlorhexidine brush during a surgery, detect operating room environmental contamination, and verify the existence of bacterial resistance to the antibiotics most commonly used in Veterinary Medicine.Materials, Methods & Results: In ten cases of the surgical routine, samples were collected concurrently at specific time points throughout 2 h of surgical procedure. To evaluate the efficacy of 2% chlorhexidine brush on hand scrubbing of a mock surgeon, swabs were used to sample the surface of the hands before and after gowning and gloving. Samples or their dilutions were inoculated onto blood agar and MacConkey agar plates. Contamination of the operating room was evaluated using BHI agar plates distributed through the room. After the incubation period, counts and biochemical tests were performed, and an antimicrobial disc susceptibility test was performed using antibiotics most commonly us

Assessment of ALA-induced PpIX production in porcine skin pretreated with microneedles.

Photodynamic therapy (PDT) is used for skin treatments of premalignant and cancer lesions and recognized as a non-invasive technique that combines tissue photosensitization and subsequent exposure to light to induce cell death. However, it is limited to the treatment of superficial lesions, mainly due to the low cream penetration. Therefore, the improvement of transdermal distribution of aminolevulinic acid (ALA) is needed. In this study, the kinetics and homogeneity of production of ALA-induced PpIX after the skin pre-treatment with microneedles rollers of 0.5, 1.0 and 1.5 mm length were investigated. An improvement in homogeneity and production of PpIX was shown in a porcine model. Widefield fluorescence imaging three hours after the topical application of ALA-cream in the combined treatment with microeedles rollers.

Endoscopic approach for a laryngeal neoplasm in a dog

Laryngeal and tracheal tumors are rare in pets; some piece of information on their disease behavior, therapy and evolution are limited. Neoplasms in this area are a diagnostic challenge. In many cases, they can be biopsied and excised using endoscopic instruments, but there is no report of this in canines. The goal of this study is to report a successful case of a laryngeal neoplasm removal through endoscopy. A head and neck radiogram revealed a mass in the laryngeal lumen protruding into the trachea. The patient then underwent an endoscopy to confirm the radiographic diagnosis and to surgically remove the tumor. The histopathological diagnosis was poorly differentiated carcinoma. The most appropriate treatment for laryngeal tumors is the resection of the submucosa or a partial laryngectomy however, partial and total laryngectomies are associated with many postoperative complications. In contrast, the endoscopic approach allows for highly magnified visualization of the lesion in situ, which facilitates the surgical removal of the mass through videosurgery. With little manipulation of the affected area, the chances of postoperative complications are reduced, leading to a more rapid recovery.

Tumores de laringe e traqueia são raros em animais de estimação e as informações sobre o comportamento, terapia e evolução destas neoplasias são limitadas. Neoplasias nesta área são desafios diagnósticos. Em muitos casos, pode ser feita a biópsia e excisão da massa utilizando instrumentos endoscópicos, mas não existe relato deste tipo de procedimento em caninos. O objetivo deste estudo foi relatar um caso de sucesso da remoção endoscópica de um tumor de laringe. A radiografia da região cervical revelou uma massa na luz da laringe invadindo a traqueia. O paciente foi então submetido a uma endoscopia para confirmar o diagnóstico radiográfico e remover cirurgicamente o tumor. O diagnóstico histopatológico foi de carcinoma pouco diferenciado. O tratamento mais adequado para os tumores da laringe é a ressecção da submucosa ou uma laringectomia parcial, no entanto, estas estão associadas a muitas complicações pós-operatórias. Em contraste, a abordagem endoscópica permite a visualização da lesão in situ, o que facilita a remoção cirúrgica da massa através de videocirurgia. Com pouca manipulação da área afetada, as chances de complicações pós-operatórias são reduzidas, levando a uma recuperação mais rápida.

Endoscopic approach for a laryngeal neoplasm in a dog

Laryngeal and tracheal tumors are rare in pets; some piece of information on their disease behavior, therapy and evolution are limited. Neoplasms in this area are a diagnostic challenge. In many cases, they can be biopsied and excised using endoscopic instruments, but there is no report of this in canines. The goal of this study is to report a successful case of a laryngeal neoplasm removal through endoscopy. A head and neck radiogram revealed a mass in the laryngeal lumen protruding into the trachea. The patient then underwent an endoscopy to confirm the radiographic diagnosis and to surgically remove the tumor. The histopathological diagnosis was poorly differentiated carcinoma. The most appropriate treatment for laryngeal tumors is the resection of the submucosa or a partial laryngectomy however, partial and total laryngectomies are associated with many postoperative complications. In contrast, the endoscopic approach allows for highly magnified visualization of the lesion in situ, which facilitates the surgical removal of the mass through videosurgery. With little manipulation of the affected area, the chances of postoperative complications are reduced, leading to a more rapid recovery.

Tumores de laringe e traqueia são raros em animais de estimação e as informações sobre o comportamento, terapia e evolução destas neoplasias são limitadas. Neoplasias nesta área são desafios diagnósticos. Em muitos casos, pode ser feita a biópsia e excisão da massa utilizando instrumentos endoscópicos, mas não existe relato deste tipo de procedimento em caninos. O objetivo deste estudo foi relatar um caso de sucesso da remoção endoscópica de um tumor de laringe. A radiografia da região cervical revelou uma massa na luz da laringe invadindo a traqueia. O paciente foi então submetido a uma endoscopia para confirmar o diagnóstico radiográfico e remover cirurgicamente o tumor. O diagnóstico histopatológico foi de carcinoma pouco diferenciado. O tratamento mais adequado para os tumores da laringe é a ressecção da submucosa ou uma laringectomia parcial, no entanto, estas estão associadas a muitas complicações pós-operatórias. Em contraste, a abordagem endoscópica permite a visualização da lesão in situ, o que facilita a remoção cirúrgica da massa através de videocirurgia. Com pouca manipulação da área afetada, as chances de complicações pós-operatórias são reduzidas, levando a uma recuperação mais rápida.

GASTROPATIA PILÓRICA HIPERTRÓFICA CRÔNICA SECUNDÁRIA À GASTRITE LINFOPLASMOCITÁRIA EM CÃO RELATO DE CASO

A gastropatia hipertrófica pilórica crônica é o termo que se refere à hipertrofia pilórica sem especificar se a camada mucosa e/ou muscular estão envolvidas. O presente trabalho relata um caso de gastropatia hipertrófica crônica secundária à gastrite linfoplasmocitária em um cão. O diagnóstico foi confirmado através de celiotomia exploratória e análise histopatológica e o tratamento instituído através de piloroplastia, bem como terapia imunossupressora foram fundamentais para a melhora clínica significativa do paciente.

GASTROPATIA PILÓRICA HIPERTRÓFICA CRÔNICA SECUNDÁRIA À GASTRITE LINFOPLASMOCITÁRIA EM CÃO RELATO DE CASO

A gastropatia hipertrófica pilórica crônica é o termo que se refere à hipertrofia pilórica sem especificar se a camada mucosa e/ou muscular estão envolvidas. O presente trabalho relata um caso de gastropatia hipertrófica crônica secundária à gastrite linfoplasmocitária em um cão. O diagnóstico foi confirmado através de celiotomia exploratória e análise histopatológica e o tratamento instituído através de piloroplastia, bem como terapia imunossupressora foram fundamentais para a melhora clínica significativa do paciente.

ASSOCIAÇÃO ENTRE A IMUNOTERAPIA INTRAVESICAL E QUIMIOTERAPIA ANTINEOPLÁSICA NO TRATAMENTO DE CARCINOMA UROTELIAL EM VESÍCULA URINÁRIA DE CÃO

O carcinoma urotelial, embora de ocorrência rara, é a neoplasia mais comum da vesícula urinária de cães. A terapia intravesical com o Bacilo de Calmette-Guérin, BCG é utilizada em carcinomas vesicais humanos com boa resposta, porém é pouco descrita em cães. O presente trabalho relata o caso de uma cadela, sem raça definida, com 10 anos de idade, portadora de carcinoma urotelial em vesícula urinária tratada com associação de quimioterapia antineoplásica, inibidor de COX-2 e BCG intravesical. O uso da imunoterapia proporcionou resultados satisfatórios, pois, enquanto administrado na paciente, interrompeu a progressão tumoral e o desenvolvimento de metástases. Trata-se, portanto, de uma modalidade promissora no controle de neoplasias.

Emprego dos bisfosfonatos em oncologia veterinária

Os bisfosfonatos (BFs) são fármacos utilizados para inibir a reabsorção óssea em diversas enfermidades que envolvem o metabolismo ósseo. Em oncologia são empregados no tratamento de tumores ósseos primários ou metastáticos e no controle da hipercalcemia maligna. Além do efeito sobre a reabsorção óssea, existem trabalhos demonstrado o efeito dos BFs diretamente sobre as células tumorais, com ação citostática, indução de apoptose, inibição da invasividade tumoral, angiogênese e interferência nos processos metastáticos. O objetivo da presente revisão é demonstrar os mecanismos de ação dos bisfosfonatos, as principais aplicações dentro da oncologia veterinária, assim como os possíveis efeitos colaterais do uso destes fármacos.

Metastasis of squamous cell carcinoma in the pelvis of a dog / Metastasis of squamous cell carcinoma in the pelvis of a dog

Background: Squamous cell carcinoma (SCC) is regarded as one of the most common diagnosis in lesions of the digit, should be included in the differential diagnosis of lesions of the digit, and seems to exhibit a distinct behavior. Metastases in bone tissue are rare. Bones such as tibia, ulna and vertebrae have been reported as carcinoma metastasis sites. However, no reports of carcinoma metastasis in pelvic bones of dogs are available. This report aims at describing a case of distant and unusual metastasis of squamous cell carcinoma in the pelvis of a dog after an attempt of surgical resection of the primary lesion in the digit. Case: A six-year-old male Rottweiler dog was referred to the Veterinary Hospital of the Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, Brazil, with a history of a non-healing lesion on the distal medial aspect of the left thoracic limb after amputation of the fi rst digit, which had been performed four months earlier owing to the same problem. Surgical excision of the lesion was performed followed by wound treatment aimed at healing by second intention as an attempt to preserve the limb. Histological analysis revealed a diagnosis of squamous cell carcinoma. There was recurrence of the lesion presented as circular areas with differentiated hemorrhagic tissue amidst the granulation tissue. Another excisional bi

Background: Squamous cell carcinoma (SCC) is regarded as one of the most common diagnosis in lesions of the digit, should be included in the differential diagnosis of lesions of the digit, and seems to exhibit a distinct behavior. Metastases in bone tissue are rare. Bones such as tibia, ulna and vertebrae have been reported as carcinoma metastasis sites. However, no reports of carcinoma metastasis in pelvic bones of dogs are available. This report aims at describing a case of distant and unusual metastasis of squamous cell carcinoma in the pelvis of a dog after an attempt of surgical resection of the primary lesion in the digit. Case: A six-year-old male Rottweiler dog was referred to the Veterinary Hospital of the Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, Brazil, with a history of a non-healing lesion on the distal medial aspect of the left thoracic limb after amputation of the fi rst digit, which had been performed four months earlier owing to the same problem. Surgical excision of the lesion was performed followed by wound treatment aimed at healing by second intention as an attempt to preserve the limb. Histological analysis revealed a diagnosis of squamous cell carcinoma. There was recurrence of the lesion presented as circular areas with differentiated hemorrhagic tissue amidst the granulation tissue. Another excisional bi

Linfadenectomia laparoscópica em cadela com neoplasia mamária

The laparoscopic lymphadenectomy is routinely performed in medicine, but not in veterinary medicine. This report describes a case of female canine with tumors in the left mammary chain (M4 and M5), which was subjected to intra-dermal application of sterile methylene blue, around the largest tumor (M5) which tried to demarcate the lymphatic vessels and regional lymph nodes. After 15 minutes, laparoscopic lymphadenectomy involving the abdominal in the left pelvic region started, followed by laparoscopic ovariohysterctomy (OVH) using three portals access. It was also performed an unilateral left total mastectomy. For histology, two abdominal lymph nodes were free of tumor cells. The patient had no recurrence in 60 days.

A linfadenectomia laparoscópica é realizada de forma rotineira na medicina, contudo é pouco utilizada na veterinária. Neste relato, um canino fêmea apresentando tumores na cadeia mamária esquerda (M4 e M5), foi submetido à aplicação intradérmica do azul de metileno estéril, ao redor do maior tumor (M5), buscando-se demarcação dos vasos linfáticos e linfonodos regionais. Após 15 minutos, iniciou-se a linfadenectomia abdominal videolaparoscópica na região inguinal esquerda, seguida da ovário-histerectomia (OVH) lapararoscópica com três portais. Realizou-se ainda mastectomia total unilateral esquerda. Pela histologia, obtiveram-se dois linfonodos abdominais livres de células tumorais. A paciente não apresentou recidiva em 60 dias.

Linfadenectomia laparoscópica em cadela com neoplasia mamária

The laparoscopic lymphadenectomy is routinely performed in medicine, but not in veterinary medicine. This report describes a case of female canine with tumors in the left mammary chain (M4 and M5), which was subjected to intra-dermal application of sterile methylene blue, around the largest tumor (M5) which tried to demarcate the lymphatic vessels and regional lymph nodes. After 15 minutes, laparoscopic lymphadenectomy involving the abdominal in the left pelvic region started, followed by laparoscopic ovariohysterctomy (OVH) using three portals access. It was also performed an unilateral left total mastectomy. For histology, two abdominal lymph nodes were free of tumor cells. The patient had no recurrence in 60 days.

A linfadenectomia laparoscópica é realizada de forma rotineira na medicina, contudo é pouco utilizada na veterinária. Neste relato, um canino fêmea apresentando tumores na cadeia mamária esquerda (M4 e M5), foi submetido à aplicação intradérmica do azul de metileno estéril, ao redor do maior tumor (M5), buscando-se demarcação dos vasos linfáticos e linfonodos regionais. Após 15 minutos, iniciou-se a linfadenectomia abdominal videolaparoscópica na região inguinal esquerda, seguida da ovário-histerectomia (OVH) lapararoscópica com três portais. Realizou-se ainda mastectomia total unilateral esquerda. Pela histologia, obtiveram-se dois linfonodos abdominais livres de células tumorais. A paciente não apresentou recidiva em 60 dias.