Metastatic adenocarcinoma arising from a small bowel duplication cyst.

A case report of adenocarcinoma arising from a small bowel mesenteric cyst is presented. A discussion and review of the relevant literature then follows.

Electroacoustic production of murine hybridomas.

The optimal conditions for the production of murine hybridomas by electroacoustic fusion of cells in sugar solutions and of cells in ionic strength media (up to 115 mM NaCl) have been investigated. In the electroacoustic fusion technique cells were brought into contact in a 3 MHz ultrasonic standing wave field and were fused by application of an electric pulse. Hybridomas of murine splenocytes and X63-Ag8.653 mouse myelomas were successfully produced in low ionic strength mannitol solutions and in a range of salt concentrations up to 115 mM. The yield of hybridomas by electroacoustic fusion of cells in mannitol was at least as good as that obtained when cells were fused following conventional dielectrophoresis induced contact. The electroacoustic fusion yield was also comparable to conventional yields when cells were exposed to a pulse in higher ionic strength media where dielectrophoresis induces heating effects. Hybridomas were produced at similar electric field strengths when cells were suspended in high ionic strength media (up to 115 mM NaCl) or in mannitol. The effective electric field strength for hybridoma production was close to that at which trypan blue tests indicated membrane damage.

Electroacoustic fusion of millilitre volumes of cells in physiological medium.

A technique is described in which erythrocytes suspended in 1.1 ml of 145 mM NaCl, have been fused by electrofusion. The cells in suspension were brought into close contact by setting up a 3 MHz ultrasonic standing wave in a cylindrical cell container. The aluminium foil base of the container served both to transmit ultrasound and as an electrode for electrofusion. The electric pulse was generated by a capacitor discharge system. The electric field strength required to fuse cells increased as the ionic strength of the cell suspending phase increased. Cells in physiological saline fused at an electric field strength of 7.3 kV/cm with a 50 microseconds pulse.

Pancreatic ischaemia; sensitivity and reversibility of the changes.

In the rat, pancreatic clamping producing warm ischaemic times ranging from 5 to 90 min gives rise to changes similar to those of an haemorrhagic pancreatitis. The severity of the changes is proportional to the time of ischaemia. After 1 to 1 1/2 h clamping 95 per cent of the glandular changes are reversible, and at 2 months the pancreas appears normal apart from localized areas of fibrosis. The islets of Langerhans remain morphologically normal even after 90 min ischaemia. The implications for islet cell transplantation are discussed.