1.
Dokl Biochem Biophys
; 440: 225-7, 2011.
Article
in English
| MEDLINE
| ID: mdl-22095125
Subject(s)
CHO Cells/metabolism , HEK293 Cells/metabolism , Oxidative Stress/drug effects , Proteins/metabolism , Animals , CHO Cells/drug effects , Cell Line , Cell Proliferation/drug effects , Cricetinae , HEK293 Cells/drug effects , Humans , Proteins/pharmacology , RNA, Small Interfering/metabolism , Recombinant Proteins/metabolism , Recombinant Proteins/pharmacology , Transfection
2.
Bioorg Khim
; 36(3): 312-8, 2010.
Article
in Russian
| MEDLINE
| ID: mdl-20644585
ABSTRACT
Haponin (HLDF-alike protein) was previously identified from the human promyelocytic leukemia HL-60 cell line. For the functional study of this protein, we obtained recombinant haponin with an N-terminal hexahistidine tag using a baculovirus expression system. Antibodies against 6xHis-haponin were produced, and the expression of endogenous haponin was demonstrated in mammalian cell lines of different origin. Using affinity chromatography and immunoprecipitation methods, we have shown that in CHO-K1 cells haponin interacts with glyceraldehyde 3-phosphate dehydrogenase (GAPDH), which is one of the vital glycolytic enzymes with a diverse set of noncanonical functions.