ABSTRACT
A 5-day-old quarter horse colt with a history of hypothermia, agonal breathing, and diarrhea was euthanized. At necropsy, numerous slightly raised, discrete, closely approximated submucosal nodules were observed in the colon and small intestine. Histologically, these nodules were composed of expanded submucosal mesenchyme that contained numerous neurons either individually or in ganglia. Thirty-two percent of these ganglia included 8 or more neurons, in contrast to 6% in an age-matched foal. Some nodules had necrosuppurative inflammation with vasculitis, thrombosis, and bacterial colonization. A few heterotopic neurons were randomly distributed in the mucosa and the muscularis mucosa. Histologic changes were most consistent with intestinal neuronal dysplasia, a disease of the submucosal plexus described in humans.
Subject(s)
Colitis/veterinary , Horse Diseases/pathology , Animals , Animals, Newborn , Colitis/pathology , Diagnosis, Differential , Fatal Outcome , HorsesABSTRACT
RATIONALE: Developmental lead exposure may alter responsiveness to cocaine well into adulthood, and ultimately influence drug-use patterns. OBJECTIVES: The present study examined the effect of perinatal lead exposure on the discriminative stimulus properties of cocaine. METHODS: Female rats were treated with 0, 8, or 16 mg lead daily for 30 days before breeding with untreated males. This exposure regimen continued through gestation and until postnatal day (PND) 21, i.e., weaning. At PND 60 male pups were trained to discriminate between saline and cocaine (5 mg/kg) injections. After acquisition, a series of generalization/substitution tests were performed using a cumulative dosing procedure. RESULTS: Developmental lead exposure produced subsensitivity to SKF-82958 (D1-like dopamine receptor agonist), quinpirole (D2-like dopamine receptor agonist), and apomorphine (mixed D1-like/D2-like dopamine receptor agonist); but no differences were evident among lead-treatment groups on generalization/substitution tests with cocaine, d-amphetamine, or GBR-12909. Furthermore, when the kappa-opioid receptor agonist U69,593 was administered prior to cocaine (5 mg/kg), generalization to the cocaine stimulus decreased in control rats, but generalization in lead-exposed rats was not altered. Group differences were not evident in tolerance or recovery of tolerance to cocaine following repeated cocaine administration (60 mg/kg per day for 14 days). Furthermore, no differences were found across groups in concentrations of lead in brain, although pups exposed to 16 mg lead had slightly elevated blood lead concentrations (<7 microg/dl). CONCLUSIONS: These results further a growing research literature that suggests developmental lead exposure can produce long-lasting changes in drug responsiveness, even after exposure to the toxicant has been discontinued.
Subject(s)
Cocaine/pharmacology , Discrimination, Psychological/drug effects , Dopamine Uptake Inhibitors/pharmacology , Organometallic Compounds/pharmacology , Prenatal Exposure Delayed Effects , Animals , Animals, Newborn , Discrimination, Psychological/physiology , Dopamine Agonists/pharmacology , Dopamine Antagonists/pharmacology , Dose-Response Relationship, Drug , Female , Male , Organometallic Compounds/metabolism , Pregnancy , Rats , Rats, Sprague-Dawley , Receptors, Opioid/agonists , Reproducibility of ResultsABSTRACT
Lead contamination of water is a major health hazard, as illustrated by the fact that exposure to this metal has been associated with death and disease in humans, birds, and animals. The present research was aimed at the development of a porous, solid-phase sorbent that can be used in the remediation of lead-contaminated water. A suitable sorbent was identified by screening various clays and other materials for their ability to effectively bind lead. The clay was adhered to a solid support using an aqueous solution of carboxymethyl cellulose. The binary composite was then tested for its ability to bind lead from solution, while providing void volume, increased surface area, and considerably enhanced hydraulic conductivity. The results suggested that a combination of sodium montmorillonite clay and carbon exhibited enhanced sorption of lead compared to carbon alone, and also supported the potential application of various combinations of sorbent materials. This value-added combination of clay, solid support, and adhesive will allow for the construction of column filtration systems that are multifunctional and capable of purifying large volumes of contaminated water.
Subject(s)
Aluminum Silicates/chemistry , Lead/pharmacokinetics , Water Pollutants, Chemical/pharmacokinetics , Water Purification/methods , Adsorption , Animals , Biological Assay , Carbon/chemistry , Carboxymethylcellulose Sodium/chemistry , Clay , Filtration , Hydra/drug effects , Lead/analysis , Lead/toxicity , Methylene Blue/pharmacokinetics , Surface Properties , Water/analysis , Water Pollutants, Chemical/analysisABSTRACT
In this study, we evaluated concentrations of twelve essential and non-essential elements (As, Cd, Co, Cu, Pb, Mg, Mn, Hg, Mo, Se, Ag, and Zn) in tissues of bowhead (Balaena mysticetus) and beluga (Delphinapterus leucas) whales from arctic Alaska (USA) and northwestern Canada. Tissue samples were collected between 1983 and 1997, mostly in 1995-97. The essential elements are reported to develop reference ranges for health status determination, and to help assess known or suspected interactions affecting toxicoses of cadmium (Cd) and mercury (Hg). In some tissues, Cd, Hg, and selenium (Se) were present at concentrations that have been associated with toxicoses in some domestic animals. Nevertheless, tissue levels of all elements were within ranges that have been reported previously in marine mammals. While mean Ag concentrations in beluga whale liver were relatively high (15.91 micrograms/g ww), Ag was not associated with hepatic Se levels or age, contrary to previous findings. Significant associations included: Cd with age, Zn, or Cu; Cu with age, Zn or Ag; and Hg with age, Se, Zn, or Cu. This study found hepatic Hg:Se molar ratios to be consistently lower than unity and different between species. Possible explanations for observed elemental correlations (i.e., interactions) and ancillary mechanisms of Cd and Hg detoxification are discussed.
Subject(s)
Metals/metabolism , Whales/metabolism , Age Factors , Alaska , Animals , Arctic Regions , Canada , Female , Kidney/chemistry , Liver/chemistry , Male , Metals/analysis , Muscles/chemistry , Reference Values , Species Specificity , Tissue DistributionABSTRACT
In this study, we evaluated concentrations of twelve essential and non-essential elements (As, Cd, Co, Cu, Pb, Mg, Mn, Hg, Mo, Se, Ag, and Zn) in tissues of ringed seals (Phoca hispida) and polar bears (Ursus maritimus) of arctic Alaska (USA). All samples were collected between 1995-97 in conjunction with subsistence harvests. The essential elements are reported to help develop reference ranges for health status determination and to help assess known or suspected interactions affecting toxicoses of cadmium (Cd) and mercury (Hg). In some tissues, Cd, Hg, and selenium (Se) were present at concentrations that have been associated with toxicoses in some domestic animals. Nevertheless, tissue levels of all elements were within ranges that have been reported previously in other pinnipeds and polar bears. Significant associations included: Cd with Zn or Cu; Cu with Zn or Ag; and Hg with Se, Zn, or Cu. This study found hepatic Hg:Se molar ratios to be lower than unity and different between the two species. Based upon significant differences in mean tissue elemental concentrations for polar bear versus ringed seal, we concluded that biomagnification factors (bear/seal) were significant for: Cu in liver and muscle; Pb in kidney; Se in kidney and muscle; Zn in liver and muscle; and Hg in liver. Possible explanations for observed elemental correlations (i.e., interactions) and ancillary mechanisms of Cd and Hg detoxification are discussed.
Subject(s)
Metals/metabolism , Seals, Earless/metabolism , Ursidae/metabolism , Adipose Tissue/chemistry , Age Factors , Alaska , Animals , Arctic Regions , Female , Food Chain , Kidney/chemistry , Liver/chemistry , Male , Metals/analysis , Muscles/chemistry , Reference Values , Species Specificity , Tissue DistributionABSTRACT
The effects of adult and perinatal lead treatment on the development of locomotor sensitization produced with repeated morphine administration was investigated. In Experiment 1, adult male rats received a diet containing 250 ppm lead acetate or a control diet for 43 days. Animals then received 10 mg/kg morphine sulfate or water vehicle (ip) and locomotor activity was monitored for 14 consecutive days. While both control and lead-exposed animals demonstrated a locomotor sensitization to morphine, the magnitude of the increased locomotor response was reduced in lead-treated animals. Subsequent analysis of blood-lead in the adult lead-exposed animals indicated residue levels ranging between 20 and 30 microg/dl. In Experiment 2, adult female rats were treated daily with 0, 8, or 16 mg lead via gavage for 30 days before breeding with non-exposed males. Lead exposure in dams continued through gestation and until pups were weaned at postnatal day (PND) 21. At PND 60, male offspring received morphine or vehicle challenges identical to those described in Experiment 1. Animals perinatally exposed to dams receiving 16 mg lead daily demonstrated an enhanced behavioral response to morphine relative to control animals. Analysis of offspring blood indicated lead levels below detectable limits (<1 microg/dl) for all animals. The results suggest exposure to lead at environmentally relevant levels produces long-lasting changes in drug-induced behavior, and the developmental period in which lead exposure occurs is a significant contributor to the manifestation of these effects.
Subject(s)
Lead/toxicity , Morphine/pharmacology , Motor Activity/drug effects , Prenatal Exposure Delayed Effects , Animals , Animals, Newborn , Behavior, Animal/drug effects , Body Weight/drug effects , Dose-Response Relationship, Drug , Eating/drug effects , Female , Lead/blood , Male , Pregnancy , Rats , Rats, Sprague-Dawley , Time FactorsABSTRACT
The purpose of this study was to examine the effects of developmental lead exposure on drug responsiveness later in the life cycle. Adult female rats were gavaged daily with 0, 8, or 16 mg lead for 30 days before breeding with non-exposed males. The respective exposure regimens were maintained throughout gestation and lactation (perinatal exposure). In Experiment 1, at postnatal day (PND) 30 or 90, pups were trained with 0, 1.25, 2.5, or 5 mg/kg cocaine HCl (IP) in a biased conditioned place preference (CPP) procedure. At both PND 30 and 90, an attenuation in CPP was present in animals exposed to 8 or 16 mg lead relative to control rats. Using an identical lead-exposure regimen, a conditioned place aversion (CPA) procedure with 0, 10, 20, or 40 mg/kg lithium chloride (IP) was employed for Experiment 2. No significant differences were present among pups from each lead-exposure group conditioned and tested at PND 30 or 90, thus suggesting that an impairment of associative mechanisms was not solely responsible for the pattern of attenuation present in Experiment 1. Subsequent analyses of blood-lead in all experiments demonstrated concentrations below 5 microg/dl for all animals at PND 30 and below detectable limits (<1 microg/dl) at PND 90. The findings suggested attenuation in cocaine reinforcement with perinatal lead exposure even though the metal apparently had gained clearance from soft tissue.
Subject(s)
Cocaine/pharmacology , Fetus/drug effects , Lead/toxicity , Reinforcement, Psychology , Animals , Conditioning, Psychological/drug effects , Dose-Response Relationship, Drug , Female , Lead/blood , Lithium Chloride/pharmacology , Male , Rats , Rats, Sprague-DawleyABSTRACT
This study examined the possibility that cadmium, a toxicant in high concentration in all tobacco products, may alter the stimulus properties of morphine. Adult male rats were exposed to regular laboratory chow (Group Control) or chow containing 100 ppm added cadmium chloride (Group Cadmium). Following an initial 30 day exposure period, control and cadmium-exposed animals were trained to discriminate between i.p. injections of 3.00 mg/kg morphine sulfate and vehicle (distilled water) in a two-choice drug discrimination task. Subsequently, the morphine dose-effect generalization function (0.75-6.00 mg/kg) was determined for control and cadmium-exposed animals. Additional substitution tests were conducted with increasing doses of the high efficacy mu agonist fentanyl (0.0016-0.04 mg/kg), the intermediate efficacy mu agonist (-)-metazocine (0.60-5.00 mg/kg), and the kappa agonist (+/-)-bremazocine (0.03-0.12 mg/kg). Also, increasing doses of the selective mu antagonist naloxone (0.0008-0.50 mg/kg) were presented against the training dose of morphine (3.00 mg/kg) and 0.02 mg/kg fentanyl. Finally, training was discontinued, and control and cadmium-exposed animals were injected with 8.00 mg/kg morphine in the home cage every 12 hr for 2 weeks, prior to redetermining the morphine dose effect function. Following a 1 week recovery period where morphine injections were discontinued, a final determination of the morphine dose-effect function was made. The results of the investigation indicated that cadmium exposure, without affecting the rate-changing properties of the drugs, slowed initial acquisition of the morphine discrimination, decreased the potency of selective doses of naloxone with respect to antagonizing the stimulus effects of morphine and fentanyl, and blocked the development of tolerance to morphine. Morphine, fentanyl, and (-)-metazocine generalized (substituted) equally across both groups, while (+/-)-bremazocine failed to substitute for the morphine stimulus in either group. These findings add to the growing literature on the interaction between metal poisoning and drug selection/abuse.
Subject(s)
Cadmium Poisoning/psychology , Discrimination Learning/drug effects , Discrimination, Psychological/drug effects , Morphine/pharmacology , Narcotics/pharmacology , Animals , Body Weight/drug effects , Cadmium/blood , Diet , Drug Tolerance , Eating/drug effects , Generalization, Stimulus/drug effects , Male , Naloxone/pharmacology , Narcotic Antagonists/pharmacology , Rats , Rats, Sprague-DawleyABSTRACT
The aim of this study was to examine the effects of perinatal lead exposure on locomotor responding following acute and repeated cocaine challenges (sensitization). Adult female rats were gavaged daily with 0, 8, or 16 mg lead acetate for 30 days prior to breeding. This exposure regimen was maintained throughout gestation and lactation (perinatal exposure). On Day 21, male pups were weaned and lead exposure was discontinued for the remainder of the study. Beginning on postnatal day (PND) 30 or PND 90, and continuing for 14 successive days, separate groups of perinatally-exposed animals were presented with challenges of 10 mg/kg cocaine HCl (i.p.), and tested for locomotor responding. Following this testing period, dose-effect profiles were determined, with animals receiving daily injections of 0, 10, 20, and 40 mg/kg cocaine. The results indicated that both at PND 30 and PND 90 lead-exposed animals were less responsive to the initial administration of cocaine, but exhibited a supersensitivity to the stimulatory effects associated with repeated administration of cocaine, i.e., behavioral sensitization to cocaine was augmented by perinatal lead exposure. Analyses of blood lead levels following the completion of testing revealed that lead levels were below detectable limits for all animals (< 1 microg/dl). Collectively, these findings show that developmental lead contamination produces changes in cocaine sensitivity long after exposure has been discontinued and the toxicant has gained clearance from blood.
Subject(s)
Cocaine/pharmacology , Dopamine Uptake Inhibitors/pharmacology , Lead/pharmacology , Motor Activity/drug effects , Prenatal Exposure Delayed Effects , Animals , Animals, Newborn , Dose-Response Relationship, Drug , Female , Male , Motor Activity/physiology , Pregnancy , Rats , Rats, Sprague-Dawley , Time FactorsABSTRACT
The apparent ability of astroglia to serve as a lead (Pb) sink in the mature brain may result from either their strategic location, between the blood-brain barrier and neurons, or from intrinsic differences between the ability of astroglia and neurons to accumulate this metal. This phenomenon may be dependent on the degree of cell differentiation. In order to address the latter possibility, Pb accumulation was compared among the following cell culture models: (1) mature and immature rat astroglia, (2) undifferentiated SY5Y human neuroblastoma cells and SY5Y cells differentiated with nerve growth factor, (3) immature rat astroglia grown in differently conditioned media, some of which induce partial differentiation, and (4) rat astroglia and SY5Y cells in co-culture. Astroglial cultures, prepared from 1-day-old rat cerebral hemispheres, were exposed to 1 microM Pb after either 14 (immature) or 21 (mature) days in culture. Pb content of the cells was measured by atomic absorption spectroscopy. Immature astroglia took up less Pb when glutathione (GSH) was added to the medium, suggesting that GSH may regulate Pb uptake in these cells. Undifferentiated neuroblastoma cells accumulated more Pb than did the differentiated ones. Astroglia accumulated up to 24 times more Pb than did neuronal cells. This ability was enhanced by exposure to conditioned medium from a neuroblastoma cell line, but not by endothelial cell-conditioned medium, although this medium induced the expression of a glutamate-activated Ca2+ response. Our findings are in agreement with in vivo studies, and thus validate the use of these cell-culture models for future studies on differential mechanisms of Pb uptake.
Subject(s)
Astrocytes/metabolism , Cell Differentiation/physiology , Lead/pharmacokinetics , Neuroblastoma/metabolism , Neurons/metabolism , Age Factors , Animals , Animals, Newborn , Brain/metabolism , Calcium/metabolism , Calcium Channels/physiology , Cells, Cultured , Glutamic Acid/pharmacology , Glutathione/pharmacology , Humans , Nerve Growth Factor/pharmacology , Rats , Rats, Sprague-Dawley , Spectrophotometry, Atomic , Tumor Cells, CulturedABSTRACT
C6 rat glioma cells resemble rat astroglia in culture in that both cell types accumulate lead (Pb) intracellularly from the medium. As such, C6 cells are a model for Pb accumulation by the brain. In this study, an increase in intracellular Pb accumulation induced by p-chloromercuribenzoate (PCMB) after exposure to 10 microM Pb acetate suggests a role for sulfhydryl groups in Pb retention. Stimulation of Pb accumulation by nifedipine suggests the entry of Pb into these cells by a novel path. Most of the intracellular Pb from exposure for 7 days to 1 microM Pb was associated with high-molecular weight components in cytosol. Pb exposure increased the abundance of three proteins with the following characteristics on two-dimensional gels: 81 kDa with pI of 5.6, 81 kDa with pI of 4. 9, and 71 kDa with pI of 5.6. The levels of five other proteins, ranging in size from 37-41 kDa with pIs of 6.0-6.8 declined. Exposed C6 cells accumulated copper (Cu) intracellularly, and Cu accumulation after Pb exposure was shown by kinetic analysis with 67Cu to result from an increased uptake and a decreased efflux for Cu. Pb-exposed cells also showed increased Cu binding to membranes, which is consistent with the increase of Cu uptake. These data indicate that intracellular Pb interacts with high molecular weight proteins in C6 cells, and exposure also alters membrane transport properties for copper.
Subject(s)
Brain Neoplasms/metabolism , Cell Membrane/metabolism , Copper/pharmacokinetics , Glioma/metabolism , Lead/toxicity , Animals , Body Burden , Calcium Channel Blockers/pharmacology , Cell Membrane/drug effects , Chromatography, Agarose , Drug Interactions , Lead/pharmacokinetics , Lead/pharmacology , Nifedipine/pharmacology , Proteins/analysis , Rats , Spectrophotometry, Atomic , Sulfhydryl Compounds/pharmacology , Tissue Distribution , Tumor Cells, Cultured , p-Chloromercuribenzoic Acid/pharmacologyABSTRACT
Astroglia serve as a presumptive lead (Pb) sink in the brain; therefore, this study examined Pb entry into cultured rat astroglia utilizing the Ca2+ fluorophore indo-1 as a tool for detecting Pb2+ entry during acute exposure. The interactions of Pb2+ with indo-1 were analyzed by fluorescence spectrophotometry in a cell-free system. The emission spectrum of Pb2+/indo-1 was substantially different from that of Ca2+/indo-1 due to suppression of indo-1 fluorescence emission intensity. Next, we established the presence of L-type Ca2+ channels in astroglial cultures and demonstrated that Pb accumulation is enhanced under serum-free conditions and by the application of Bay-K 8644. Because acute exposure is of less toxicologic relevance than repeated low-level exposure, we then examined Pb uptake in cultures treated for up to 1 week with Pb. AAS revealed that Pb accumulation was accompanied by an increase in total cellular [Ca]. In addition, differences in basal indo-1 fluorescence levels and differences in responsiveness to ionomycin were observed. Ionomycin induced an increase in the fluorescence ratio in untreated cells but cells treated for 1 day with Pb showed no response to ionomycin. However, cells treated for 3 and 7 days showed a partial response to ionomycin. TPEN was used to evaluate the interactions of Pb2+ with indo-1 and only cells treated for 7 days showed a response to TPEN. Thus, the present study characterizes Pb2+ entry into astroglia via L-type Ca2+ channels and presents the possibility of using indo-1 for analysis of Pb2+ uptake and the subsequent neurotoxic events in astroglia.
Subject(s)
Astrocytes/metabolism , Lead/metabolism , Animals , Animals, Newborn , Calcium/metabolism , Calcium Channels/metabolism , Calcium Channels, L-Type , Cells, Cultured , Cerebral Cortex/cytology , Cerebral Cortex/drug effects , Cerebral Cortex/metabolism , Chelating Agents/pharmacology , Culture Media, Serum-Free , Ethylenediamines/pharmacology , Fluorescent Dyes , Indoles , Kinetics , Lead/toxicity , Rats , Rats, Sprague-Dawley , Spectrophotometry, AtomicABSTRACT
This study evaluated the use of IEC-6 cells as a model for studying lead (Pb) transport by intestinal epithelial cells (IECs) and examined potential transport mechanisms for Pb uptake and extrusion. Pb accumulation in IEC-6 cells exposed to 5 and 10 microM Pb for up to 60 min was time- and dose-dependent. Reduction of incubation temperature significantly reduced the total cellular Pb content of IEC-6 cells. Simultaneous exposed of cells to zinc (Zn) and Pb resulted in decreased total cellular Pb contents compared to total cellular Pb contents of cells exposed to Pb only. IEC-6 cells treated with ouabain (1 mM) or sodium azide (1 mM) and 5 microM Pb accumulated more Pb than cells exposed to Pb only. Cells treated with p-chloromercuribenzensulfonic acid (50 microM), p-chloromercuribenzoic acid (50 microM), or iodoacetimide (50 microM) accumulated less Pb than cells treated with Pb only. We conclude that Pb uptake by IEC-6 cells depends on the extracellular Pb concentration. Our data suggest that the mechanism of Pb uptake by IECs is complex, and that Pb transport in IEC-6 cells is time- and temperature-dependent, involves sulfhydryl groups, and is decreased by the presence of Zn. Extrusion of Pb is at least partially dependent on metabolic energy.
Subject(s)
Intestinal Mucosa/metabolism , Lead/pharmacokinetics , Animals , Biological Transport/drug effects , Cell Line , Intestinal Mucosa/cytology , Rats , Sulfhydryl Compounds/pharmacology , Temperature , Zinc/pharmacokineticsABSTRACT
Previous investigations of metal/cocaine interactions have shown that chronic oral exposure to inorganic lead or cadmium attenuates the psychoactive effects of acute or repeated administration of cocaine. The purpose of this investigation was to assess the possibility that such interactive effects may derive from metal-induced disturbances in cocaine pharmacokinetics, i.e., delivery of cocaine to critical biologic sites may be disrupted by metal contamination. In this study, adult male rats were exposed to purified diets containing 250 ppm lead acetate (Group Lead), 100 ppm cadmium chloride (Group Cadmium), or unadulterated laboratory chow (Group Control); n = 48/exposure condition. Following ad libitum access to their respective diets in the home cage for 45 days, half the animals from each exposure regimen received single daily IP injections of 5, 10, or 20 mg/kg cocaine HCl for a period of 7 days (n = 8/group). The remaining half the animals received repeated daily injections of saline during this pretreatment phase. On the day following pretreatment, animals previously receiving cocaine injections were administered a single cocaine test challenge at a dose equal to that received in pretreatment. Similarly, saline pretreatment animals received either 5, 10, or 20 mg/kg cocaine. The results of this investigation did not reveal reliable evidence of metal-related differences in brain levels of cocaine. Plasma cocaine and benzoylecgonine (BE) levels also were essentially the same for control and metal-exposed animals. The failure to show that lead or cadmium alters the disposition of cocaine in brain or plasma underscores the need to pursue alternative accounts of metal/cocaine interactions.
Subject(s)
Brain/metabolism , Cadmium Chloride/toxicity , Cocaine/analogs & derivatives , Cocaine/blood , Narcotics/blood , Organometallic Compounds/toxicity , Animals , Body Weight/drug effects , Brain/drug effects , Cadmium Chloride/blood , Cocaine/administration & dosage , Corpus Striatum/metabolism , Dose-Response Relationship, Drug , Drug Interactions , Eating/drug effects , Injections, Intraperitoneal , Male , Narcotics/administration & dosage , Nucleus Accumbens/metabolism , Rats , Rats, Sprague-DawleyABSTRACT
Chronic lead exposure has been shown to attenuate cocaine-induced increases in extracellular dopamine levels in the region of the nucleus accumbens, and antagonize the locomotor stimulating effects of the drug. The purpose of this study was to determine if similar lead-induced disturbances in the effects of cocaine include the impact of the drug on schedule-controlled responding. Adult male rats exposed ad libitum to water containing 500 ppm lead acetate (Group Lead), or a comparable concentration of sodium acetate (Group Control), were placed on a restricted diet (12-15 g food/day) prior to commencing fixed-interval (F1-5 min) schedule training on Day 33 of exposure. After 27 days of operant training, animals received a sequence of no injection, saline injection, and cocaine injection tests, repeating the sequence for 3, 10, 20 and 40 mg/kg cocaine HCl (i.p.). Local rates were determined for successive 30 s segments of the interval and the pattern of responding was compared under conditions of saline and cocaine injection. For both groups, cocaine increased responding, especially early in the interval. However, the rate enhancing effects of cocaine were less pronounced in lead-exposed animals than controls, at least at the 20 mg/kg dose. These data extend earlier findings and accent the need to examine further the interactive relations between the external chemical environment and drug sensitivity.
Subject(s)
Cocaine/pharmacology , Conditioning, Operant/drug effects , Lead Poisoning/psychology , Narcotics/pharmacology , Animals , Body Weight/drug effects , Drinking/drug effects , Male , Rats , Rats, Sprague-Dawley , Reinforcement ScheduleABSTRACT
Employing a paired-watering procedure to control for differential fluid intake confounds, adult male rats were exposed in the home cage to water containing 100 ppm cadmium chloride, or a control solution containing no added cadmium chloride. On Day 61 of exposure to their respective watering regimens, half the animals from each condition received 12 repeated daily i.p. injections of 10 mg/kg cocaine-HCl, or saline. Locomotor activity (total distance traveled) was recorded in Digiscan Activity Monitors for a 20-min baseline period prior to each injection, and for a 40-min period post-injection. On Day 13 of testing, all animals received saline injections only in the test chambers, in an effort to evaluate the role of conditioned cues in the expression of cocaine sensitization. On Day 14-16 of testing, all animals received successive daily challenges of 10, 20, and 40 mg/kg cocaine in the test chamber. The results indicated that the initiation (development) of behavioral sensitization to 10 mg/kg cocaine was attenuated in cadmium-exposed rats. Moreover, the supersensitivity to higher doses of cocaine during dose-effect testing that was registered by control animals pretreated with cocaine, was not evident in cadmium-exposed pretreatment animals. These data suggests that environmental contaminants may alter drug responsiveness, and thereby may influence patterns of drug selection and use.
Subject(s)
Behavior, Animal/drug effects , Cadmium/pharmacology , Cocaine/pharmacology , Animals , Body Weight/drug effects , Dose-Response Relationship, Drug , Drinking/drug effects , Male , Rats , Rats, Sprague-Dawley , Time FactorsABSTRACT
Twenty-four adult male rats were exposed in the home cage to water containing 100 ppm added cadmium chloride. An additional 24 animals were pair-watered with water containing no added cadmium. Following 60 days of exposure to their respective watering regimens, one third of the animals in each exposure group (N = 8/condition) received IP injections of 1.0, 2.0, or 3.0 g/kg ethanol (20% v/v). Serum alcohol concentrations were measured at 15, 30, 60, 120, 180, 240, and 360 min postinjection. Although serum alcohol concentrations increased with dose for both cadmium-exposed and control animals, there was no indication at any dose of group differences. The lack of differences in ethanol pharmacokinetics reported here is instructive with respect to improving our understanding of the mechanisms underlying cadmium/ethanol interactions.
Subject(s)
Cadmium/pharmacology , Ethanol/pharmacokinetics , Animals , Body Weight/drug effects , Cadmium/blood , Drinking/drug effects , Ethanol/blood , Male , Rats , Rats, Sprague-DawleyABSTRACT
The purpose of this study was to help characterize the pathway of copper in the liver, kidney and duodenum during copper loading and unloading in the rat. Male Wistar rats were allocated randomly into four groups: Group A (control) was composed of 16 animals fed a normal rodent diet. Group B had 16 animals fed a high copper diet (1500 ppm copper). Four rats from each group were killed at 1, 5, 10 and 15 weeks. Group C had 4 animals fed the high copper diet for 5 weeks and normal diet for 5 weeks. Group D consisted of 4 animals fed the high copper diet for 5 weeks, normal diet for 5 weeks, followed by 5 weeks of high copper diet. At termination of each experimental period liver, kidney and duodenum were collected for histochemistry and copper analysis by atomic absorption spectrophotometry. Hepatic copper concentration in Group B rose to 726 +/- 170 micrograms/g after 5 weeks; renal and duodenal copper levels were 285 +/- 14 micrograms/g and 134 +/- 49 micrograms/g, respectively. A significant (P < 0.005) decrease in copper concentration was observed after 15 weeks in all three organs. Duodenal copper concentration in group C was similar to control rats. Changes in copper tissue distribution and efficient unloading were demonstrated in all copper-loaded groups in the three organs studied.
Subject(s)
Copper/metabolism , Duodenum/metabolism , Kidney/metabolism , Liver/metabolism , Animals , Body Weight , Copper/administration & dosage , Copper/analysis , Copper/toxicity , Data Interpretation, Statistical , Duodenum/chemistry , Histocytochemistry , Kidney/chemistry , Liver/chemistry , Male , Random Allocation , Rats , Rats, Wistar , Time FactorsABSTRACT
Adult male rats were exposed to drinking fluid containing either 500 ppm lead acetate (group lead), or an equivalent concentration of sodium acetate (group control) for 61 days prior to pain reactivity testing using a tail-flick procedure. Rats were placed in restraining tubes for a 20 min acclimation period, and then baseline tail-flick latencies in response to a radiant heat source were measured. Subsequently, half the animals from each group were serially injected IP with either 1.0, 2.0, or 3.0 g/kg body weight of a 20% v/v ethanol solution, and the other half were injected with an equivalent volume of saline. Tail-flick latencies were reassessed at 20-min intervals over the next 2 h. Results indicated dose-dependent ethanol-induced hypoalgesia at all doses, but at the two higher doses the magnitude of the hypoalgesic response was significantly greater in the group control animals than in the group lead animals across the 2-h postinjection period. Results are discussed in terms of an attenuation of the pharmacological properties of ethanol by lead.
Subject(s)
Analgesics/pharmacology , Ethanol/pharmacology , Lead Poisoning/psychology , Analgesics/antagonists & inhibitors , Animals , Body Weight/drug effects , Dose-Response Relationship, Drug , Drinking/drug effects , Lead/blood , Male , Pain Measurement/drug effects , Rats , Rats, Sprague-Dawley , Reaction Time/drug effectsABSTRACT
In the present study, we evaluated the in vitro effects of lead (Pb) on basal and stimulated luteinizing hormone releasing hormone (LHRH) and Prostaglandin E2 (PGE2) secretion. Median eminences (ME) were removed from brains of adult male rats and preincubated for 15 minutes in Krebs-Ringer bicarbonate glucose buffer in an atmosphere of 95% O2-5% CO2. These media were discarded and all MEs were subjected to one of the following experiments. In Experiment 1, all MEs were incubated for 30 minutes in medium only. These media were collected and replaced with medium only (controls) or with medium containing Pb doses ranging from 5 to 20 microM. After this 60-minute incubation, media were collected, then replaced with new medium containing 60 microM norepinephrine (NE), or NE plus each dose of Pb, then incubated for a final 30-minute period. Experiment 2 was conducted as above, except PGE2 (2.8 microM) replaced the NE. In both experiments, the amounts of LHRH released was measured by RIA. In experiment 3, NE was again used for the challenge; however, this time, the amount of PGE2 released was measured by RIA. Results indicate that Pb did not alter basal LHRH release, but compared with controls, significantly blocked NE-induced LHRH release in a dose-related manner. Conversely, Pb had no effect on the PGE2-induced release of LHRH. Additionally, Pb did not alter basal PGE2 release; however, it significantly blocked the NE-induced release of PGE2. Since NE-induced LHRH release is mediated by PGE2, these results support the hypothesis that Pb is capable of altering the hypothalamus and suggest that this effect is due, at least in part, to the diminished PGE2 synthesis/release within the ME, resulting in diminished LHRH secretion.