ABSTRACT
The effect of systemic administration of (-)-nicotine on release of noradrenaline in the hippocampus was studied by in vivo microdialysis in freely moving rats, using dialysate containing nomifensine (5 microM). (-)-Nicotine, at both 0.4 and 0.8 mg/kg but not 0.2 mg/kg, rapidly and significantly increased extracellular levels of noradrenaline. Extracellular levels of dopamine were also increased, but this was only significant after the larger dose. Both 0.4 and 0.8 mg/kg also produced a significant increase in extracellular levels of the metabolites of dopamine, 3,4-dihydroxyphenylacetic acid and homovanillic acid. Extracellular levels of the metabolite of 5-hydroxytryptamine, 5-hydroxyindoleacetic acid, increased after 0.8 mg/kg but this effect was only apparent much later. Injection of a second 0.8 mg/kg challenge of (-)-nicotine, 150 min after the first, produced similar increases in extracellular levels of noradrenaline, dopamine, 3-4-dihydroxyphenylacetic acid and homovanillic acid. Over the experimental period, there was no further increase in extracellular levels of 5-hydroxyindoleacetic acid. Increases in extracellular levels of noradrenaline, dopamine, 3,4-dihydroxyphenylacetic acid and homovanillic acid, in response to 0.8 mg/kg (-)-nicotine, were prevented by the systemic administration of mecamylamine, but not hexamethonium (both at 5 mg/kg). Mecamylamine also inhibited the delayed increase in extracellular levels of 5-hydroxyindoleacetic acid, produced by the first injection of (-)-nicotine. These results suggest that (-)-nicotine, dose-dependently stimulated the release and metabolism of amine transmitters by an action at central nicotinic receptors. However, the precise site of action, i.e. at nerve terminals, cell bodies or both, requires further elucidation.
Subject(s)
Hippocampus/drug effects , Nicotine/pharmacology , Norepinephrine/metabolism , 3,4-Dihydroxyphenylacetic Acid/metabolism , Animals , Behavior, Animal , Dopamine/metabolism , Dose-Response Relationship, Drug , Hexamethonium , Hexamethonium Compounds/pharmacology , Hippocampus/metabolism , Homovanillic Acid/metabolism , Male , Mecamylamine/pharmacology , Nicotine/administration & dosage , Rats , Rats, Inbred StrainsABSTRACT
Using in vivo dialysis and voltammetry, the effect of acute administration of (-)-nicotine (0.8 mg/kg free base, s.c.) on extracellular levels of dopamine, 3,4-dihydroxyphenylacetic acid, 5-hydroxy-indoleacetic acid and ascorbic acid in the nucleus accumbens and caudate-putamen of chloral hydrate-anaesthetised rats has been examined. Nicotine stimulated release of dopamine only in the nucleus accumbens, measured using dialysis. After a short time delay levels of 3,4-dihydroxyphenylacetic acid in both the nucleus accumbens and caudate-putamen also increased. In both regions, 5-hydroxyindoleacetic acid was unaffected by nicotine. Using voltammetry the effect of nicotine on extracellular levels of 3,4-dihydroxyphenylacetic acid and ascorbic acid was examined. An increase in 3,4-dihydroxyphenylacetic acid was observed in both regions after nicotine. This increase was blocked by pretreatment with the central nicotinic receptor antagonist mecamylamine (5 mg/kg). Nicotine increased the level of ascorbic acid in the nucleus accumbens and caudate-putamen; while in animals pretreated with mecamylamine, nicotine decreased levels of ascorbate. These results show that acute administration of nicotine stimulated release of dopamine in the nucleus accumbens and increased the levels of DOPAC and ascorbic acid in the nucleus accumbens and caudate-putamen. This effect is probably mediated by nicotinic receptors as it was antagonised by mecamylamine.
Subject(s)
3,4-Dihydroxyphenylacetic Acid/metabolism , Ascorbic Acid/metabolism , Caudate Nucleus/physiology , Dopamine/metabolism , Nicotine/pharmacology , Nucleus Accumbens/physiology , Putamen/physiology , Animals , Caudate Nucleus/drug effects , Caudate Nucleus/metabolism , Evoked Potentials/drug effects , Male , Mecamylamine/pharmacology , Nucleus Accumbens/drug effects , Nucleus Accumbens/metabolism , Organ Specificity , Putamen/drug effects , Putamen/metabolism , Rats , Rats, Inbred Strains , Reference Values , Time FactorsABSTRACT
The role of projections from coerulear (A6) and lateral tegmental (A1-A5) noradrenergic cell groups in the induced catecholamine response to (-)-nicotine was studied following lesions to the dorsal (DNAB) or ventral (VNAB) noradrenergic bundle by 6-hydroxydopamine. The lesions produced large reductions in basal noradrenaline levels in hippocampus (after DNAB lesions) and hypothalamus (after VNAB lesions), while not affecting basal levels of dopamine or 5-hydroxytryptamine. Vehicle and sham operated controls showed a significant increase in DOPA accumulation in response to (-)-nicotine (0.8 mg/kg s.c.) following inhibition of amino acid decarboxylase. In DNAB lesioned rats, the response induced by (-)-nicotine in both the hippocampus and hypothalamus was significantly attenuated, whereas in VNAB lesioned rats the induced response was still evident. The effect of (-)-nicotine was also studied in the nucleus accumbens and was found not to be affected by either lesion. These data suggest that increases in catecholamine synthesis in the hippocampus and hypothalamus reflect increased noradrenaline synthesis, and that this effect occurs specifically in noradrenergic projections originating in the locus coeruleus.
Subject(s)
Brain Chemistry/drug effects , Catecholamines/biosynthesis , Nicotine/pharmacology , Animals , Chromatography, High Pressure Liquid , Hippocampus/cytology , Hippocampus/physiology , Hydroxydopamines/pharmacology , Hypothalamus/cytology , Hypothalamus/physiology , Locus Coeruleus/cytology , Locus Coeruleus/physiology , Male , Norepinephrine/physiology , Nucleus Accumbens/drug effects , Nucleus Accumbens/metabolism , Oxidopamine , Rats , Rats, Inbred Strains , Sympathectomy, ChemicalABSTRACT
Acute nicotine treatment increases catecholamine synthesis selectively in the nucleus accumbens, hypothalamus and hippocampus of the rat. In the hippocampus, but not in the other areas mentioned, or in the caudate, chronic pretreatment with nicotine for 28 days potentiates this response. In the present study we have investigated the effects of acute treatment with the same dose of nicotine (0.8 mg/kg s.c.), and of chronic pretreatment with this dose, on the in vitro activity of tyrosine hydroxylase, the rate limiting enzyme for catecholamine synthesis. Tyrosine hydroxylase was not altered by acute nicotine, but was increased by about 2-fold, selectively in the hippocampus, by the chronic pretreatment. These results show that nicotine treatment increases the capacity for noradrenaline synthesis in the hippocampus; taken with our previous results they indicate that this capacity is not manifest until an acute challenge occurs. This effect of nicotine on tyrosine hydroxylase is consistent with nicotinic receptor mediation of central transsynaptic induction.
ABSTRACT
Acute (-)-nicotine administration (0.4 and 0.8 mg/kg s.c.) produced a regionally specific increase in the rate of catecholamine synthesis in the rat nucleus accumbens, hypothalamus and hippocampus but not elsewhere, including the caudate-putamen. In all regions rates of 5-hydroxytryptamine synthesis were unaffected. (-)-Cotinine (0.4 and 0.8 mg/kg), the major metabolite of (-)-nicotine was without effect. (-)-Nicotine-induced increase in catecholamine synthesis occurred by a direct stimulation of central nicotinic receptors, as mecamylamine (5 mg/kg) but not hexamethonium (5 mg/kg) was an effective antagonist. Following repeated daily injections of (-)-nicotine (0.8 mg/kg) for up to 28 days, the induced catecholamine response following a subsequent challenge was unaffected in the nucleus accumbens and hypothalamus, but was increased in the hippocampus. This effect persisted for up to 14 days following withdrawal. Rates of 5-hydroxytryptamine synthesis remained unaltered after chronic pretreatment.
Subject(s)
Brain Chemistry/drug effects , Catecholamines/biosynthesis , Nicotine/pharmacology , Serotonin/biosynthesis , Animals , Behavior, Animal/drug effects , Dihydroxyphenylalanine/metabolism , Hexamethonium Compounds/pharmacology , Male , Mecamylamine/pharmacology , Rats , Rats, Inbred Strains , Stereoisomerism , Time FactorsABSTRACT
Pressure-ejection of 100 mM KCl was used to induce voltammetric signals in the rat caudate. The signals, detected chronoamperometrically with Nafion-coated carbon fiber microelectrodes, were reproducibly generated at 20-min intervals up to distances of 1600 micron from the KCl stimulus site. Unilateral 6-hydroxydopamine lesions of the substantia nigra (SN) demonstrated that over 90% of the voltammetric signal generated was dopamine. Evaluation of the signal onset at two widely spaced electrodes suggested that injection of nl volumes of 100 mM KCl into the rat caudate generates voltammetric signals which resemble spreading depression (SD) produced by more classical methods (e.g. 1 M KCl). We further investigated this phenomenon by simultaneous evaluation of extracellular K+ ion concentration changes, field potential (FP) and voltammetric signals or multiunit activity following stimulation with 100 mM or 1 M KCl. The results show that the signals generated by 100 mM KCl have many of the attributes of 'classical' SD, although the extracellular K+ ion concentration changes and FP changes were smaller in magnitude. However, the characteristic burst of multiunit activity followed by a marked quiescent period found during 1 M KCl stimulation was not observed with 100 mM KCl stimulation. Furthermore, application of 0.5% lidocaine to the SN reversibly blocked all signals generated by 100 mM KCl in the caudate while similar treatment with up to 2% lidocaine was ineffective when 1 M KCl was used as the stimulus. The results suggest that the signals generated by 100 mM KCl may represent an attenuated form of SD which requires a functioning SN, and that this stimulation could be a useful model for studying neurotransmitter interactions in the propagation of the SD phenomena.
Subject(s)
Caudate Nucleus/physiology , Dopamine/physiology , Lidocaine/pharmacology , Neural Inhibition/drug effects , Potassium Chloride/pharmacology , Substantia Nigra/physiology , Action Potentials/drug effects , Animals , Caudate Nucleus/drug effects , Dopamine/metabolism , Hydroxydopamines , Male , Oxidopamine , Rats , Rats, Inbred Strains , Substantia Nigra/drug effectsABSTRACT
A method which improves carbon fiber microelectrode selectivity for cationic amines by electrocoating the fiber with a thin film of the ionic polymer, Nafion, is described. The selectivity and response speed of these electrodes for the detection of electroactive cationic and anionic species found in brain extracellular fluid was evaluated using differential pulse voltammetry and chronoamperometry and compared to uncoated fibers. Carbon fiber microelectrodes electrocoated with Nafion are highly sensitive to cationic amines such as dopamine and serotonin and have minimal sensitivity to anions such as ascorbic acid and uric acid at physiological concentrations.
Subject(s)
Carbon , Electrolysis , Electroplating , Fluorocarbon Polymers , Microelectrodes/standards , Neurotransmitter Agents/metabolism , ElectrochemistrySubject(s)
Brain/physiology , Neurotransmitter Agents/physiology , 3,4-Dihydroxyphenylacetic Acid/metabolism , Animals , Antipsychotic Agents/pharmacology , Blood Pressure , Brain/drug effects , Dialysis , Dopamine/metabolism , Electrochemistry , Electrodes , Epinephrine/physiology , Hydroxyindoleacetic Acid/metabolism , Medulla Oblongata/physiology , Rats , Receptors, Serotonin/physiology , Serotonin/metabolismABSTRACT
K+-stimulated release of [3H]-5-hydroxytryptamine ( [3H]-5-HT) from rat frontal cortex slices was decreased by the 5-HT receptor agonists 5-methoxy-n1N-dimethyltryptamine and 5-methoxy-3(1,2,3,6,-tetrahydro-4-pyrindinyl)-1H-indole (RU-24969) (1 X 10(-5)M). RU-24969 (10 mg kg-1, i.p.) decreased extracellular 5-HT and its metabolite 5-hydroxyindoleacetic acid measured in vivo by use of intracerebral dialysis combined with high performance liquid chromatography and electrochemical detection. The decrease in extracellular 5-hydroxyindoleacetic acid in vivo after RU-24969 (10 mg kg-1, i.p.) was also observed by in vivo voltammetry. The non-selective 5-HT antagonist metergoline prevented the RU-24969-induced decrease in 5-HT release and metabolism in vivo while the 5-HT2 receptor antagonist R-55669 (ritanserin) did not. The results support the view that RU-24969 stimulates a 5-HT1 receptor that is involved in the autoregulation of 5-HT release and metabolism.
Subject(s)
Frontal Lobe/metabolism , Indoles/pharmacology , Receptors, Serotonin/drug effects , Serotonin/metabolism , Animals , Chromatography, High Pressure Liquid , Dialysis , Electrochemistry , Hydroxyindoleacetic Acid/metabolism , In Vitro Techniques , Male , Metergoline/pharmacology , Rats , Rats, Inbred Strains , Time FactorsABSTRACT
A direct comparison has been made of the drug-induced changes in extracellular levels of 3,4-dihydroxyphenylacetic acid and 5-hydroxyindoleacetic acid measured using intracerebral dialysis and differential pulse voltammetry with carbon fibre electrodes. The comparison was carried out in chloral hydrate anaesthetized rats with a pretreated carbon fibre electrode implanted in one striatum and an intracerebral dialysis loop in the contralateral striatum. 3,4-Dihydroxyphenylacetic acid and 5-hydroxyindoleacetic acid in the dialysis perfusates were assayed by high pressure liquid chromatography with electrochemical detection. d-Amphetamine (2 mg/kg) decreased extracellular 3,4-dihydroxyphenylacetic acid and the height of the 3,4-dihydroxyphenylacetic acid oxidation peak, while haloperidol (0.5 mg/kg) increased 3,4-dihydroxyphenylacetic acid levels measured in the perfusates and the height of the 3,4-dihydroxyphenylacetic acid oxidation peak. In these experiments there were parallel changes in 3,4-dihydroxyphenylacetic acid levels and peak height and a close correlation between these changes. Tranylcypromine (10 mg/kg) produced an almost parallel decrease in extracellular 5-hydroxyindoleacetic acid (dialysis) and the height of the 5-hydroxyindoleacetic acid oxidation peak, with similar percentage changes and good correlation values being obtained. However, while 5-hydroxy-L-tryptophan (25 mg/kg) increased both the 5-hydroxyindoleacetic acid levels and the height of the 5-hydroxyindoleacetic acid oxidation peak, 5-hydroxyindoleacetic acid in the dialysis perfusates showed a greater increase than the oxidation peak. The results show a close correlation between changes in extracellular 3,4-dihydroxyphenylacetic acid and its respective voltammetric peak and strongly support the use of in vivo differential pulse voltammetry for monitoring dopamine metabolism.(ABSTRACT TRUNCATED AT 250 WORDS)
Subject(s)
3,4-Dihydroxyphenylacetic Acid/analysis , Corpus Striatum/analysis , Hydroxyindoleacetic Acid/analysis , Phenylacetates/analysis , 5-Hydroxytryptophan/pharmacology , Animals , Chromatography, High Pressure Liquid , Dialysis , Electrochemistry/methods , Male , Rats , Rats, Inbred Strains , Tranylcypromine/pharmacologyABSTRACT
The effects of injection of the thyrotrophin-releasing hormone (TRH) analogue, orotyl-histidyl-proline amide (CG3509) into the accumbens and striatum, was studied on dopamine metabolism by means of in vivo voltammetry. Forty minutes after infusion of CG3509 (1-5 micrograms) into the n. accumbens there was a significant dose-related increase in the oxidation peak, corresponding to the oxidation of the dopamine metabolite, dihydroxyphenylacetic acid (DOPAC) and ascorbic acid. The size of this peak returned to normal by 80 min after the infusion. There was no change in the indole oxidation peak. Infusion of CG3509 (5 micrograms) had no effect on the size of either the catechol or the indole oxidation peaks recorded in the striatum. Intraventricular injection of CG3509 (10 micrograms) also increased DOPAC/ascorbic acid oxidation peak recorded in the n. accumbens, without altering the indole peak. While the voltammetric technique used in the present experiments is not able fully to separate ascorbic acid and DOPAC in vivo, the results support the view that TRH and its analogues selectively increase dopaminergic activity in the mesolimbic region.
Subject(s)
Ascorbic Acid/metabolism , Catechols/metabolism , Corpus Striatum/drug effects , Nucleus Accumbens/drug effects , Septal Nuclei/drug effects , Thyrotropin-Releasing Hormone/analogs & derivatives , 3,4-Dihydroxyphenylacetic Acid/metabolism , Animals , Corpus Striatum/metabolism , Dopamine/metabolism , In Vitro Techniques , Male , Nucleus Accumbens/metabolism , Oxidation-Reduction , Rats , Rats, Inbred Strains , Thyrotropin-Releasing Hormone/pharmacologySubject(s)
Ascorbate Oxidase/pharmacology , Ascorbic Acid/metabolism , Corpus Striatum/drug effects , Nucleus Accumbens/drug effects , Oxidoreductases/pharmacology , Septal Nuclei/drug effects , 3,4-Dihydroxyphenylacetic Acid/metabolism , Animals , Dopamine/metabolism , Electrochemistry , Electrodes , Male , Oxidation-Reduction , RatsABSTRACT
1 Differential pulse voltammetric scans recorded using graphite paste working electrodes implanted in the striatum and frontal cortex of anaesthetized rats exhibited up to three distinct oxidation peaks at potentials between -0.1 and +0.5 V. 2 The first peak at about +0.12 V was selectively increased by the micro-infusion of ascorbic acid (5 X 10(-6) M) close to the surface of the working electrode implanted in either the striatum or the frontal cortex. 3 The second peak at about +0.22 V was selectively increased by micro-infusing either dopamine or 3,4-dihydroxyphenylacetic acid, (5 X 10(-6) M). 4 The third peak at approximately + 0.35 V was selectively increased in a dose-related manner by the micro-infusion of either 5-hydroxytryptamine, (7.5 X 10(-6) M to 7.5 X 10(-5) M) or 5-hydroxyindole-3-acetic acid, (2.5 X 10(-5) M to 6 X 10(-4) M). 5 The results show that differential pulse voltammetry can be used to obtain qualitative and quantitative information about catechol and indoleamine neurones in vivo.