Metals profile in deep-sea sediment from an active tectonic region around Simeulue Island, Aceh, Indonesia.

Simeulue waters are adjacent to the northern part of Sumatra Island, which is undergoing massive land-use transformations; moreover, the waters are located in an active tectonic region. Land changes and tectonic activity might affect the metal pollution profile in this deep sea area. Thus, this study aimed to investigate the vertical profile and assess the sediment quality from the deep-sea marine sediment around Simeulue Island based on metal concentration. Seventy-six bottom sediment samples were collected from eight cores at a water depth of up to 2800 m in the Simeulue waters, Indonesia, in November 2017. Metals Cd, Cu, Fe, Ni, Pb, and Zn were quantified from the cores and multivariate analyses were carried out to understand the process. Metals distributions are analogous to the grain size parameters and LOI550 distribution pattern, while Sumatra and Simeulue islands influenced grain size and LOI550 spatial distribution. The vertical grain size profile exhibited no extreme oscillation in the investigated cores. Thus, sediment transport from the Island was the main suspect for these metals' profiles in the deep water, and the tectonic activity had a minor impact. Cu, Ni, Pb, and Zn tend to rise in the collected cores, suggesting that the accumulation of the metals is growing. While Fe tended to be stable and Cd oscillated in the cores. Indices were computed to assess the metal contamination profile. The cores were dominated by EF class 1 (none to slight enrichment) status and Igeo class 1 (unpolluted). Cd was the metal of concern in the study since a high Cd was observed in some layers (maximum EF = 26.45 and maximum Igeo = 3.81). Thus, this study can be used as a database to improve the regulation formulation for improved environmental managerial efforts in the region.

Preliminary study of geochemical speciation of copper and nickel in coastal sediments in Surabaya, Indonesia.

Surabaya is one of the big coastal cities in Indonesia with rapid municipal development. Thus, the investigation on the metal's geochemical speciation in the coastal sediment is required to assess the environmental quality by studying their mobility, bioavailability, and toxicity. This study is aimed at evaluating the condition of the Surabaya coast by assessing copper and nickel fractionations and total concentrations of both metals in sediments. Environmental assessments were performed by using geo-accumulation index (Igeo), contamination factor (CF), and pollution load index (PLI) for existing total heavy metal data and by using individual contamination factor (ICF) and risk assessment code (RAC) for metal fractionations. Copper speciation was observed geochemically in the fraction order of residual (9.21 - 40.08 mg/kg) > reducible (2.33 - 11.98 mg/kg) > oxidizable (0.75 - 22.71 mg/kg) > exchangeable (0.40 - 2.06 mg/kg), while the detected fraction order of nickel was residual (5.16 - 13.88 mg/kg) > exchangeable (2.33 - 5.95 mg/kg) > reducible (1.42 - 4.74 mg/kg) > oxidizable (1.62 - 3.88 mg/kg). Different fraction levels were found for nickel speciation wherein its exchangeable fraction was higher than copper, even though the residual fraction was dominant for both copper and nickel. The total metal concentrations of copper and nickel were found in the range of 13.5 - 66.1 mg/kg dry weight and 12.7 - 24.7 mg/kg dry weight, respectively. Despite the fact that almost all index values are detected low through total metal assessment, the port area is indicated to be in the moderate contamination category for copper. Through the assessment of metal fractionation, copper is classified into the low contamination and low-risk category, while nickel is categorized into the moderate contamination level and medium risk to the aquatic environment. Although the coast of Surabaya generally remains in the safe category for living habitat, certain sites had relatively high metal concentrations estimated to have originated from anthropogenic activities.

The pro-apoptotic activity of sinueracasbanone D isolated from Sinularia Leptoclados in hepatocellular carcinoma cells.

The soft-bodied corals of the genera Sarcophyton and Sinularia (Alcyoniidae) are known as a warehouse of casbane and cembranoid diterpenoids with remarkable antitumor effects. Two casbane-type diterpenoids (1, 2) along with four cembrane-type diterpenoids (3-6) were isolated from the diethyl ether soluble fraction of the organic extracts of the Red Sea soft corals Sinularia leptoclados and Sarcophyton glaucum, respectively. The antiproliferative activity of all isolated compounds (1-6) against three hepatocellular carcinoma cells, namely, Huh-7, SNU 499, and HepG2, along with the normal cells EA.hy 926, was evaluated. Sinueracabanone D (1) displayed a remarkable antiproliferative effect against the examined cancer cell lines, especially HepG2 cells with IC50 of 4.0 ± 0.37 µM. Cell cycle analysis indicated compound 1 caused the accumulation of HepG2 cells in the G2/M-phase. Further, compound 1 exhibited significant pro-apoptotic activities in HepG2 cells as evidenced by annexin V staining, enhanced mRNA expression of Bax, cytochrome C, and caspase 3, as well as inhibition of Bcl2 expression. Also, challenging HepG2 cells with sinueracabanone D (1) enhanced the active oxygen species generation and decreased mitochondrial membrane potential. In conclusion, compound 1 possesses potent antiproliferative activities against HepG2 cells. These antiproliferative activities are mediated, at least partly, by their ability to induce apoptosis, mitochondrial dysfunction, and oxidative stress.

Exploring the Mangrove Fruit: From the Phytochemicals to Functional Food Development and the Current Progress in the Middle East.

Nowadays, the logarithmic production of existing well-known food materials is unable to keep up with the demand caused by the exponential growth of the human population in terms of the equality of access to food materials. Famous local food materials with treasury properties such as mangrove fruits are an excellent source to be listed as emerging food candidates with ethnomedicinal properties. Thus, this study reviews the nutrition content of several edible mangrove fruits and the innovation to improve the fruit into a highly economic food product. Within the mangrove fruit, the levels of primary metabolites such as carbohydrates, protein, and fat are acceptable for daily intake. The mangrove fruits, seeds, and endophytic fungi are rich in phenolic compounds, limonoids, and their derivatives as the compounds present a multitude of bioactivities such as antimicrobial, anticancer, and antioxidant. In the intermediary process, the flour of mangrove fruit stands as a supplementation for the existing flour with antidiabetic or antioxidant properties. The mangrove fruit is successfully transformed into many processed food products. However, limited fruits from species such as Bruguiera gymnorrhiza, Rhizophora mucronata, Sonneratia caseolaris, and Avicennia marina are commonly upgraded into traditional food, though many more species demonstrate ethnomedicinal properties. In the Middle East, A. marina is the dominant species, and the study of the phytochemicals and fruit development is limited. Therefore, studies on the development of mangrove fruits to functional for other mangrove species are demanding. The locally accepted mangrove fruit is coveted as an alternate food material to support the sustainable development goal of eliminating world hunger in sustainable ways.

Assessment of potential variability of cadmium and copper trace metals using hindcast estimates.

Trace metals are vital to primary productivity and play an essential role as main components in regulating oceanic biogeochemical cycles. Dissolved and particulate trace metals within the water column may vary due to primary production, temperature, and nutrient changes, factors that may also vary spatially and temporally. Furthermore, assessment of trace metals mainly relies on in situ observation, and so wide-area investigation of trace-metal concentration may be challenging and subject to technical constraints. A specific approach is therefore necessary that combines biogeochemical proxies, satellite data, and trace-metal linear correlation. This study aims to assess the potential spatio-temporal variability of sea surface cadmium (Cd) and copper (Cu) concentrations in Indonesian seas and surrounding areas. The correlations of Cd and Cu concentrations with primary production and nutrient data were used to convert hindcast satellite data into estimates of the metals' concentrations. The potential variability of trace metals can be determined by overlaying both data. Indonesia's Fisheries Management Areas (FMAs) were used for data clustering and analysis. The results show that Cd and Cu trace metals have similar distribution patterns throughout the year. However, dissolved Cu has a more diverse coverage area than dissolved Cd, including within the Halmahera, Seram, and Maluku Seas (FMAs 716 and 717), the Makassar Strait (FMA 717), and the Java-Sumatra upwelling area (FMA 573). Both Cd and Cu concentrations in the Java-Sumatra upwelling region follow the periodic upwelling pattern. Overall, both Cd and Cu show a declining trend in concentration from 2012 to 2019. It is estimated that dissolved Cd concentration declined from 1500-2000 pmol/kg in 2012 to 1000-1500 pmol/kg in 2019 for all locations. Dissolved Cu concentration decreased from 30-35 nmol/kg in 2012 to 25-30 nmol/kg in 2019. Estimated dissolved Cd and Cu follow the linear functions of silicate (SiO4), nitrate (NO3), and primary productivity. The fluctuation of anthropogenic activities and global warming are likely to indirectly impact the decline in metal concentrations by affecting nutrients and primary productivity.

Antiproliferative Isoprenoid Derivatives from the Red Sea Alcyonacean Xenia umbellata.

From the soft coral Xenia umbellata, seven isoprenoid derivatives were isolated, including a new xenicane diterpene, xeniolide O (5) and a new gorgostane derivative gorgst-3ß,5α,6ß,11α,20(S)-pentol-3-monoacetate (7), along with three known sesquiterpenes (1-3), a known diterpene (4), and a known steroid (6). The extensive analyses of the NMR, IR, and MS spectral data led to determination of their chemical structures. Compounds 1-7 displayed a cytotoxic effect against breast adenocarcinoma (MCF-7), hepatocellular carcinoma (HepG2), and cervix adenocarcinoma (HeLa), with IC50 values ranging between 1.5 ± 0.1-23.2 ± 1.5; 1.8 ± 0.1-30.6 ± 1.1 and 0.9 ± 0.05-12.8 ± 0.5 µg/mL, respectively. Compound 3 showed potent cytotoxic effects against MCF-7, HepG2, and HeLa with IC50 values = 2.4 ± 0.20, 3.1 ± 0.10 and 0.9 ± 0.05 µg/mL, respectively. Compounds 2, 5, and 7 displayed cytotoxic effect against Hela cells with IC50 values = 12.8 ± 0.50, 6.7 ± 1.00 and 11.5 ± 2.20 µg/mL, respectively. Two DNA binding dyes, acridine orange (AO) and ethidium bromide (EtBr) were used for the detection of viable, apoptotic, and necrotic cells. The early apoptotic cell death was observed in all types of treated cells. The late apoptotic cells were highly present in HepG2 cells. Compounds 5 and 7 induced a high percentage of necrosis towards HepG2 and HeLa cells. The late apoptosis was recorded as a high rate after treatment with 7 on all cancer cells.