Scanning electron microscopic study of the lingual papillae in the Anatolian water buffalo / Estudio de las papilas linguales en el búfalo de agua de anatolia por microscopía electrónica de barrido

The purpose of this study was to describe the surface structure of the lingual papillae in Anatolian Water Buffaloes using SEM. Six male Anatolian Water Buffaloes were used. Filiform, lentiform and conical papillae were determined three types as mechanical papillae. Fungiform and vallate papillae were observed two types as gustatory papillae on the tongue in Anatolian Water Buffalo. The filiform papillae were observed on the apex and body of the tongue, besides randomly identified lateral surface of the body. The conical papillae differed from the filiform papillae with its larger size and non existence of the secondary papillae. The fungiform papillae was round in shape and mushroom-like, scattering among the filiform papillae on the dorsal surface of the apex, body and the root of the tongue. The fungiform papillae's taste pores and creter-like structure were determined. The lentiform papillae were determined in two types. The borders of the cells and the micro-pitted appearance were defined at the lentiform papillae. In the vallate papillae bud fosse and a thick annular pad was found. These differences on the morphological structures of the lingual papillae were considered to be related to genetic diversity.

El objetivo de este estudio fue describir la estructura de la superficie de las papilas linguales en Búfalos de agua de Anatolia mediante microscopía electrónica de barrido (MEB). Se utilizaron seis Búfalos machos para el estudio. Se determinaron tres tipos de papilas mecánicas: filiformes, lenticular y cónicas. También se encontraron papilas gustativas de dos tipos: fungiformes y caliciformes. Las papilas filiformes se localizaron en el vértice y cuerpo de la lengua, además de encontrarse con una distribución al azar en la superficie lateral del cuerpo lingual. Las papilas cónicas se diferenciaron de las filiformes por su mayor tamaño y la ausencia de papilas secundarias. Las papilas fungiformes presentaban una forma redonda, similar a un hongo, con una distribución entre las papilas filiformes en la superficie dorsal del vértice, cuerpo y la raíz de la lengua. Además se observaron poros gustatorios en las papilas fungiformes con estructura similar de cráter. Las papilas lenticulares se determinaron en dos tipos. Se definieron los márgenes celulares y aspecto de micro corrosión en las papilas lenticulares. En las papilas circunvaladas se observaron brotes excavados y una almohadilla anular gruesa. Se consideró que estas diferencias en las estructuras morfológicas de las papilas linguales están relacionadas con la diversidad genética.

Effect of ozone oxidative preconditioning in preventing early radiation-induced lung injury in rats

Ionizing radiation causes its biological effects mainly through oxidative damage induced by reactive oxygen species. Previous studies showed that ozone oxidative preconditioning attenuated pathophysiological events mediated by reactive oxygen species. As inhalation of ozone induces lung injury, the aim of this study was to examine whether ozone oxidative preconditioning potentiates or attenuates the effects of irradiation on the lung. Rats were subjected to total body irradiation, with or without treatment with ozone oxidative preconditioning (0.72 mg/kg). Serum proinflammatory cytokine levels, oxidative damage markers, and histopathological analysis were compared at 6 and 72 h after total body irradiation. Irradiation significantly increased lung malondialdehyde levels as an end-product of lipoperoxidation. Irradiation also significantly decreased lung superoxide dismutase activity, which is an indicator of the generation of oxidative stress and an early protective response to oxidative damage. Ozone oxidative preconditioning plus irradiation significantly decreased malondialdehyde levels and increased the activity of superoxide dismutase, which might indicate protection of the lung from radiation-induced lung injury. Serum tumor necrosis factor alpha and interleukin-1 beta levels, which increased significantly following total body irradiation, were decreased with ozone oxidative preconditioning. Moreover, ozone oxidative preconditioning was able to ameliorate radiation-induced lung injury assessed by histopathological evaluation. In conclusion, ozone oxidative preconditioning, repeated low-dose intraperitoneal administration of ozone, did not exacerbate radiation-induced lung injury, and, on the contrary, it provided protection against radiation-induced lung damage.

Effect of ozone oxidative preconditioning in preventing early radiation-induced lung injury in rats.

Ionizing radiation causes its biological effects mainly through oxidative damage induced by reactive oxygen species. Previous studies showed that ozone oxidative preconditioning attenuated pathophysiological events mediated by reactive oxygen species. As inhalation of ozone induces lung injury, the aim of this study was to examine whether ozone oxidative preconditioning potentiates or attenuates the effects of irradiation on the lung. Rats were subjected to total body irradiation, with or without treatment with ozone oxidative preconditioning (0.72 mg/kg). Serum proinflammatory cytokine levels, oxidative damage markers, and histopathological analysis were compared at 6 and 72 h after total body irradiation. Irradiation significantly increased lung malondialdehyde levels as an end-product of lipoperoxidation. Irradiation also significantly decreased lung superoxide dismutase activity, which is an indicator of the generation of oxidative stress and an early protective response to oxidative damage. Ozone oxidative preconditioning plus irradiation significantly decreased malondialdehyde levels and increased the activity of superoxide dismutase, which might indicate protection of the lung from radiation-induced lung injury. Serum tumor necrosis factor alpha and interleukin-1 beta levels, which increased significantly following total body irradiation, were decreased with ozone oxidative preconditioning. Moreover, ozone oxidative preconditioning was able to ameliorate radiation-induced lung injury assessed by histopathological evaluation. In conclusion, ozone oxidative preconditioning, repeated low-dose intraperitoneal administration of ozone, did not exacerbate radiation-induced lung injury, and, on the contrary, it provided protection against radiation-induced lung damage.