Cd exposure confers ß-cypermethrin tolerance in Lymantria dispar by activating the ROS/CnCC signaling pathway-mediated P450 detoxification.

Heavy metal pollutants are important abiotic environmental factors affecting pest habitats. In this study, Cd pre-exposure significantly increased the tolerance of Lymantria dispar larvae to ß-cypermethrin, but did not significantly alter their tolerance to λ-cyhalothrin and bifenthrin. The activation of P450 by Cd exposure is the key mechanism that induces insecticide cross-tolerance in L. dispar larvae. Both before and after ß-cypermethrin treatment, Cd exposure significantly increased the expression of CYP6AB224 and CYP6AB226 in L. dispar larvae. Silencing CYP6AB224 and CYP6AB226 reduced the tolerance of Cd-treated L. dispar larvae to ß-cypermethrin. Transgenic CYP6AB224 and CYP6AB226 genes significantly increased the tolerance of Drosophila and Sf9 cells to ß-cypermethrin, and the recombinant proteins of both genes could significantly metabolise ß-cypermethrin. Cd exposure significantly increased the expression of CnCC and Maf. CnCC was found to be a key transcription factor regulating CYP6AB224- and CYP6AB226-activated insecticide cross-tolerance in Cd-treated larvae. Decreasing reactive oxygen species (ROS) levels in the Cd-treated larvae or increasing ROS levels in the untreated larvae reduced or enhanced the expression of CnCC, CYP6AB224 and CYP6AB226 and ß-cypermethrin tolerance in L. dispar larvae, respectively. Collectively, Cd exposure confers ß-cypermethrin tolerance in L. dispar larvae through the ROS/CnCC signalling pathway-mediated P450 detoxification.

Cadmium exposure through the food chain reduces the parasitic fitness of Chouioia cunea to Hyphantria cunea pupae: An ecotoxicological risk to pest control.

The toxicity of heavy metals on various trophic levels along the food chain has been extensively investigated, but no studies have focused on parasitic natural enemy insects. Herein, we constructed a food chain consisting of soil-Fraxinus mandshurica seedlings-Hyphantria cunea pupae-Chouioia cunea to analyze the effects of Cd exposure through food chain on the fitness of parasitic natural enemy insects and its corresponding mechanism. The results showed that the transfer of Cd between F. mandshurica leaves and H. cunea pupae and between H. cunea pupae and C. cunea was a bio-minimization effect. After parasitizing Cd-accumulated pupa, the number of offspring larvae, and the number, individual size (body weight, body length, abdomen length) and life span of offspring adults decreased significantly, while the duration of embryo development extended significantly. The contents of malondialdehyde and H2O2 in Cd-exposed offspring wasps increased significantly, accompanied by a significantly decrease in antioxidant capacity. The cellular immunity parameters significantly decreased in Cd-accumulated pupae, including the number of hemocytes, melanization activity and the expression level of cellular immunity genes (e.g. Hemolin-1 and PPO1). The humoral immunity disorder was found in the Cd-accumulated pupae, as evidenced by that the expression level of immune recognition gene (PGRP-SA), signal transduction genes (IMD, Dorsal, and Tube), as well as all antimicrobial peptide genes (e.g. Lysozym and Attacin) decreased significantly. Cd exposure decreased the content of glucose, trehalose, amino acid, and free fatty acid in H. cunea pupae. The expression of Hk2 in glycolysis pathway and the expression of Idh2, Idh3, Cs, and OGDH in TCA cycle pathway were significantly down-regulated in Cd-accumulated pupae. Taken together, exposure to Cd through the food chain causes oxidative damage on the offspring wasps and disrupts energy metabolism of the host insect, ultimately reducing the parasitic fitness of C. cunea to H. cunea pupae.

Defense response of Fraxinus mandshurica seedlings to Hyphantria cunea larvae under Cd stress: A contradiction between attraction and resistance.

Heavy metal pollution, as a common and serious environmental problem worldwide, has been regarded as an abiotic stimulus that can affect plant insect resistance and pest occurrence. This study evaluated the defense response of Fraxinus mandshurica seedlings to Hyphantria cunea larvae under Cd stress, with consideration given to chemical defense, physical defense, and elemental defense. Our results showed that the H. cunea larvae had a strong preference for Cd-treated F. mandshurica seedlings, but there was a significant reduction in body weight and survival rate in larvae that fed on leaves of Cd-treated seedlings. Under Cd treatment, the increase in attractant metabolites (e.g., styrene, dibutyl phthalate, and d-limonene) and the decrease in repellent metabolites (e.g., aromadendrene, heptadecane, and camphene) in leaf volatiles were responsible for the high attractant activity to H. cunea larvae. Based on leaf physicochemical properties, tissue structure, and phenolic acid content, an overall reduction in physical defense, chemical defense and their combination in F. mandshurica seedlings exposed to Cd stress was identified by Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS) analysis. Elemental defense occurred in Cd-treated F. mandshurica seedlings, as evidenced by the high concentration of Cd in leaves and H. cunea larvae under Cd treatment. Taken together, these findings demonstrate that under Cd stress, elemental defense replaces the dominant role of basic defense in F. mandshurica seedlings and accounts for the enhanced ability to defend against H. cunea larvae.