ABSTRACT
Dominant microorganisms and functional genes, including hgcA, hgcB, merA, and merB, have been identified to be responsible for mercury (Hg) methylation or methylmercury (MeHg) demethylation. However, their in situ correlation with MeHg levels and the processes of Hg methylation and MeHg demethylation in coastal areas remains poorly understood. In this study, four functional genes related to Hg methylation and MeHg demethylation (hgcA, hgcB, merA, and merB) were all detected in the sediments of the Eastern China Coastal Seas (ECCSs) (representative coastal seas highly affected by human activities) using metagenomic approaches. HgcA was identified to be the key gene controlling the in situ net production of MeHg in the ECCSs. Based on metagenomic analysis and incubation experiments, sulfate-reducing bacteria were identified as the dominant microorganisms controlling Hg methylation in the ECCSs. In addition, hgcA gene was positively correlated with the MeHg content and Hg methylation rates, highlighting the potential roles of Hg methylation genes and microorganisms influenced by sediment physicochemical properties in MeHg cycling in the ECCSs. These findings highlighted the necessity of conducting similar studies in other natural systems for elucidating the molecular mechanisms underlying MeHg production in aquatic environments.
Subject(s)
Geologic Sediments , Methylmercury Compounds , Oceans and Seas , Water Pollutants, Chemical , Geologic Sediments/microbiology , Methylmercury Compounds/metabolism , China , Water Pollutants, Chemical/metabolism , Bacteria/metabolism , Bacteria/genetics , Biodegradation, Environmental , Methylation , Mercury/metabolism , Genes, BacterialABSTRACT
The Paris Agreement and the Minamata Convention on Mercury are two of the most important environmental conventions being implemented concurrently, with a focus on reducing carbon and mercury emissions, respectively. The relation between mercury and carbon influences the interactions and outcomes of these two conventions. This perspective investigates the link between mercury and CO2, assessing the consequences and exploring the policy implications of this link. We present scientific evidence showing that mercury and CO2 levels are negatively correlated under natural conditions. As a result of this negative correlation, the CO2 level under the current mercury reduction scenario is predicted to be 2.4-10.1 ppm higher than the no action scenario by 2050, equivalent to 1.0-4.8 years of CO2 increase due to human activity. The underlying causations of this negative correlation are complex and need further research. Economic analysis indicates that there is a trade-off between the benefits and costs of mercury reduction actions. As reducing mercury emission may inadvertently undermine efforts to achieve climate goals, we advocate for devising a coordinated implementation strategy for carbon and mercury conventions to maximize synergies and reduce trade-offs.
Subject(s)
Carbon Dioxide , Mercury , Humans , Mercury/analysis , Policy , ClimateABSTRACT
Antibiotic resistance is one of the most concerned global health issues. However, comprehensive profiles of antibiotic resistance genes (ARGs) in various environmental settings are still needed to address modern antibiotic resistome. Here, Arctic soils and representative contaminated samples from ARG pollution sources were analyzed using metagenomic approaches. The diversity and abundance of ARGs in Arctic soils were significantly lower than those in contaminated samples (p < 0.01). ARG profiles in Arctic soils were featured with the dominance of vanF, ceoB, and bacA related to multidrug and bacitracin, whereas those from ARG pollution sources were characterized by prevalent resistance to anthropogenic antibiotics such as sulfonamides, tetracyclines, and beta-lactams. Mobile genetic elements (MGEs) were found in all samples, and their abundance and relatedness to ARGs were both lower in Arctic soils than in polluted samples. Significant relationships between bacterial communities and ARGs were observed (p < 0.01). Cultural bacteria in Arctic soils had clinically-concerned resistance to erythromycin, vancomycin, ampicillin, etc., but ARGs relevant to those antibiotics were undetectable in their genomes. Our results suggested that Arctic environment could be an important reservoir of novel ARGs, and antibiotic stresses could cause ARG pollution via horizontal gene transfer and enrichment of resistant bacteria.
Subject(s)
Anti-Bacterial Agents , Genes, Bacterial , Anti-Bacterial Agents/pharmacology , Soil , Bacteria/genetics , AmpicillinABSTRACT
Metagenomic binning is an essential technique for genome-resolved characterization of uncultured microorganisms in various ecosystems but hampered by the low efficiency of binning tools in adequately recovering metagenome-assembled genomes (MAGs). Here, we introduce BASALT (Binning Across a Series of Assemblies Toolkit) for binning and refinement of short- and long-read sequencing data. BASALT employs multiple binners with multiple thresholds to produce initial bins, then utilizes neural networks to identify core sequences to remove redundant bins and refine non-redundant bins. Using the same assemblies generated from Critical Assessment of Metagenome Interpretation (CAMI) datasets, BASALT produces up to twice as many MAGs as VAMB, DASTool, or metaWRAP. Processing assemblies from a lake sediment dataset, BASALT produces ~30% more MAGs than metaWRAP, including 21 unique class-level prokaryotic lineages. Functional annotations reveal that BASALT can retrieve 47.6% more non-redundant opening-reading frames than metaWRAP. These results highlight the robust handling of metagenomic sequencing data of BASALT.
Subject(s)
Ecosystem , Metagenome , Silicates , Metagenome/genetics , Metagenomics/methodsABSTRACT
Lead (Pb) is a pervasive toxic metal contaminant associated with a high risk of myocardial injury. However, the precise mechanism underlying Pb-induced myocardial injury has yet to be fully elucidated. In this study, a murine model of Pb exposure (0, 1, 5, and 10 mg/kg) was employed to investigate the involvement of neutrophil degranulation in the induction of myocardial injury. Notably, serum levels of cardiac troponin I (cTnI) and creatine kinase-MB (CK-MB) increased significantly in Pb-exposed mice, whereas cTnI levels in cardiomyocytes decreased, suggesting that Pb exposure may cause early myocardial injury. Moreover, Pb exposure was found to promote neutrophil degranulation, as evidenced by elevated myeloperoxidase (MPO) and neutrophil elastase (NE) concentrations in both the serum of Pb-exposed workers and Pb-exposed mice, as well as the extracellular supernatant of neutrophils following exposure. However, we found that serum level of cTnI enhanced by Pb exposure is associated with increased NE levels in the serum, but not with MPO levels. Upon treatment with NE inhibitor (sivelestat), the serum level of cTnI markedly reduced in Pb-exposed mice, we found that early myocardial injury is associated with NE levels in the serum. At the molecular level, western blotting analysis revealed an upregulation of ERK1/2 expression in vitro following Pb exposure, suggesting that the activation of the ERK1/2 signaling pathway may underlie the participation of neutrophil degranulation in Pb-induced myocardial injury. In summary, our findings demonstrate that Pb exposure can initiate early myocardial injury by promoting the neutrophil degranulation process, thereby highlighting the potential role of this process in the pathogenesis of Pb-associated myocardial injury.
Subject(s)
Lead , Neutrophils , Mice , Animals , Neutrophils/metabolism , Lead/toxicity , Myocytes, Cardiac/metabolism , Leukocyte Elastase/metabolismABSTRACT
Intestinal cell metabolism plays an important role in intestine health. Perfluorooctanoic acid (PFOA) exposure could disorder intestinal cell metabolism. However, the mechanisms regarding how the three carbon sources interact under PFOA stress remined to be understood. The present study aimed to dissect the interconnections of glucose, glutamine, and fatty acids in PFOA-treated human colorectal cancer (DLD-1) cells using 13C metabolic flux analysis. The abundance of glycolysis and tricarboxylic acid (TCA) cycle metabolites was decreased in PFOA-treated cells except for succinate, whereas most of amino acids were more abundant. Beside serine and glycine, the levels of metabolites derived from 13C glucose were reduced in PFOA-treated cells, and the pentose phosphate pathway flux was 1.4-fold higher in PFOA-treated cells than in the controls. In reductive glutamine pathway, higher labeled enrichment of citrate, malate, fumarate, and succinate was observed for PFOA-treated cells. The contribution of glucose to fatty acid synthesis in PFOA-treated cells decreased while the contribution of glutamine to fatty acid synthesis increased. Additionally, synthesis of TCA intermediates from fatty acid ß-oxidation was promoted in PFOA-treated cells. All results suggested that metabolic remodeling could happen in intestinal cells exposed to PFOA, which was potentially related to PFOA toxicity relevant with the loss of glucose in biomass synthesis and energy metabolism.
Subject(s)
Fatty Acids , Glutamine , Humans , Glutamine/metabolism , Glucose/metabolism , Intestines , SuccinatesABSTRACT
The sea surface microlayer (SML) is the uppermost â¼1000 µm of the surface of the ocean. With distinct physicochemical properties and position relative to the adjacent subsurface waters (SSWs), the ubiquitous distribution and high dynamics of the SML greatly regulate the global air-sea gas exchange and biogeochemistry. Mercury (Hg) redox chemistry in surface seawaters and air-sea exchange of gaseous Hg (mainly Hg(0)) fundamentally control the global oceanic Hg cycle. However, the occurrence and transformation of Hg in the SML have been poorly quantified. Here we optimize the traditional SML sampling system to make it more suitable for dissolved gaseous Hg (DGM, mainly Hg(0)) sampling. We then assess the temporal and spatial variability of DGM, total Hg, dissolved organic carbon (DOC), and Hg redox chemistry in the SML and SSWs of diverse marine environments. Our data suggest a general DGM, total Hg, and DOC enrichment in the SML relative to the SSWs but with complex variability in time and space. The incubation experiments further reveal the complex characteristics of Hg redox chemistry between the SML and SSWs. We discuss important implications of the SML Hg cycle on air-sea Hg exchange and suggest wider investigations of the SML Hg cycle in the global hydrosphere.
Subject(s)
Mercury , Water Pollutants, Chemical , Gases/analysis , Gases/chemistry , Mercury/analysis , Oceans and Seas , Seawater/chemistry , Environmental Monitoring , Water Pollutants, Chemical/analysisABSTRACT
The coastal streams (CSs) and sewage outfalls (SOs) are widely distributed and direct anthropogenic stress on global coastal ecosystems. However, the CS/SO-associated mercury (Hg) discharge, pollution and cycle in nearshore environment are less quantified. Here, we report that total Hg (THg) and methylmercury (MMHg) concentrations in waters of CSs (n = 8) and SOs (n = 15) of the northern China were â¼102 to 103 times of coastal surface waters and 10 to 102 times of major rivers in China and other regions. The CS/SO discharges resulted in the increase of total organic carbon (TOC) contents, THg and MMHg concentrations and TOC-normalized THg and MMHg concentrations in sediments of CS/SO-impacted coasts. The laboratory experiments further illustrated that the CS/SO-impacted sediments characterized with high potentials of dissolved THg and MMHg productions and releases. Our findings indicate that the layout optimization of SOs is able to reduce the Hg risk in coastal environment.
ABSTRACT
Lead (Pb) exposure is well recognized as a significant environmental factor associated with the high incidence of cardiovascular diseases. However, the carriers and molecular targets of Pb in human blood remain to be understood, especially for a real Pb exposure scenario. In this study, a total of 350 blood samples were collected from the smelting workers and systematically analyzed using metallomics and metalloproteomics approaches. The results showed that the majority of Pb (â¼99.4%) could be presented in the blood cells. Pb in the cytoplasm of blood cells accounted for approximately 83.1% of the total blood Pb, with nearly half of Pb being bound to proteins. Pb-binding proteins in the blood of workers were identified as hemoglobin, catalase, haptoglobin, δ-aminolevulinic acid dehydratase, and peroxiredoxin-2. Multiple linear regression analysis demonstrated that higher levels of Pb bound to proteins (Mix-bound Pb and Protein-bound Pb) were positively associated with higher systolic blood pressure (p < 0.05). However, the association between blood lead level, Pb levels in the blood cells and systolic blood pressure was not observed (p > 0.05). This study suggested that Pb bound to proteins could be a suitable biomarker for indicating the potential risk of occupational hypertension.
Subject(s)
Cardiovascular Diseases , Hypertension , Humans , Carrier Proteins , Blood Pressure , Lead/toxicityABSTRACT
Gaseous mercury (mainly elemental mercury, Hg(0)) exchange between air and Earth's surfaces is one of the most critical fluxes governing global Hg cycle. As an important and unique part of intertidal ecosystem, tidal flat is characterized by periodic inundation and exposure due to tidal cycle, generating varying hydrological, photochemical and biogeochemical processes. However, quantitative and mechanistic understanding of Hg(0) dynamics between air and exceptionally dynamic tide flats has remained limited to date. In this study, we select five representative tidal flat sediments from typical coastal habits of Chinese coastlines to perform laboratory incubation experiments for deciphering the effect of the interaction of tidal cycle and solar radiation on Hg(0) dynamics over tidal flats with different sediment compositions. We show that sediment Hg concentration, tidal cycle and solar radiation collectively modulate the air-surface Hg(0) exchange over tidal flats and highlight that the photochemistry dominates the Hg(0) production and emission over tidal flats. We find that the daytime inundation presents highest Hg(0) emission fluxes for Hg-poor sediment, but the daytime exposure is the hot moment of Hg(0) emission from Hg-rich sediments and substantially contributes to daily Hg(0) emission fluxes. In the treatment to mimic semidiurnal tide, the daily Hg(0) fluxes are positively correlated to sediment Hg concentrations. Combining our mechanistic insights on air-surface Hg(0) exchange over tidal flats and related data and knowledge reported by other studies, we discuss the implications of our study for field measurement and model development of Hg(0) dynamics over highly dynamic tidal flats. We conclude that the air-surface Hg(0) dynamics over tidal flats are extremely complex and highly variable, and a greater understanding the interactions between natural processes, human impacts and climate forcings will better constrain current and future Hg biogeochemical cycle in global tidal flats.
Subject(s)
Mercury , Humans , Mercury/analysis , Gases/analysis , Ecosystem , Climate , Environmental MonitoringABSTRACT
Coastal ecosystem is an important zone of mercury (Hg) storage and hotspot of neurotoxic methylmercury (MMHg) production and bioaccumulation. The releases of Hg from coastal aquifer or subterranean estuary (STE) via submarine groundwater discharge (SGD) to coastal waters provide an important source of Hg from land to seas. However, the transport and biogeochemical transformation of Hg in STEs are less quantified. In this study, we documented total Hg (THg) and MMHg dynamics in two distinct STEs (a sandflat at an open coast versus a mudflat at a bay) during two different seasons (December versus August) in the temperate coast of northern China, and further applied the salinity-based conservative mixing model (CMM) to quantify the coupling effect of hydrological and biogeochemical processes on STE Hg cycle. Our field data presented large variations of THg and MMHg concentrations and%MMHg/THg of groundwater and sediment in both STEs over time and space. The CMM results clearly displayed substantial divergences of dissolved THg and MMHg from salinity in groundwater between sites and seasons, and the concentration and percent deviations in the Hg-rich mudflat were significantly higher than those in the Hg-poor sandflat. Our findings indicate the non-conservative mixing behaviors of Hg along the groundwater flow paths of both STEs, and the Hg-rich intertidal zone could be hotspot for the production and source of dissolved THg and MMHg to coastal waters via SGD. Our results provide field evidence to highlight that the hydrological shifts and biogeochemical processes collectively drive complex transport and biogeochemical transformation of Hg in STEs. The non-conservative mixing behaviors of Hg in STEs also highlight that, for more accurately calculating SGD-derived Hg fluxes to coastal seas, we need to carefully select the groundwater zonation of STE to better represent the output endmember. Our findings also address that human activities and climate change will profoundly alter the Hg biogeochemical cycle and toxicology in global coastal aquifers.
Subject(s)
Groundwater , Mercury , Humans , Mercury/analysis , Seawater , Estuaries , Ecosystem , Environmental Monitoring/methods , Water MovementsABSTRACT
Human exposure to arsenic via drinking water is one of globally concerned health issues. Oxidative stress is regarded as the denominator of arsenic-inducing toxicities. Therefore, to identify intracellular sources of reactive oxygen species (ROS) could be essential for addressing the detrimental effects of arsenite (iAsIII). In this study, the contributions of different pathways to ROS formation in iAsIII-treated human normal liver (L-02) cells were quantitatively assessed, and then concomitant oxidative impairs were evaluated using metabolomics and lipidomics approaches. Following iAsIII treatment, NADPH oxidase (NOX) activity and expression levels of p47phox and p67phox were upregulated, and NOX-derived ROS contributed to almost 60.0 % of the total ROS. Moreover, iAsIII also induced mitochondrial superoxide anion and impaired mitochondrial respiratory function of L-02 cells with a decreasing ATP production. The inhibition of NOX activity significantly rescued mitochondrial membrane potential in iAsIII-treated L-02 cells. Purine and glycerophospholipids metabolisms in L-02 cells were disrupted by iAsIII, which might be used to represent DNA and plasma membrane damages, respectively. Our study supported that NOX could be the primary pathway of ROS overproduction and revealed the potential mechanisms of iAsIII toxicity related to oxidative stress.
Subject(s)
Arsenic , Arsenites , Humans , Reactive Oxygen Species/metabolism , Arsenites/toxicity , Liver/metabolism , Cell Membrane/metabolism , DNAABSTRACT
Arsenic (As) is one of the most concerning elements due to its high exposure risks to organisms and ecosystems. The interaction between arsenicals and proteins plays a pivotal role in inducing their biological effects on living systems, e.g., arsenicosis. In this review article, the recent advances in analytical techniques and methods of As-binding proteomes were well summarized and discussed, including chromatographic separation and purification, biotin-streptavidin pull-down probes, in situ imaging using novel fluorescent probes, and protein identification. These analytical technologies could provide a growing body of knowledge regarding the composition, level, and distribution of As-binding proteomes in both cells and biological samples, even at the organellar level. The perspectives on analysis of As-binding proteomes are also proposed, e.g., isolation and identification of minor proteins, in vivo targeted protein degradation (TPD) technologies, and spatial As-binding proteomics. The application and development of sensitive, accurate, and high-throughput methodologies of As-binding proteomics would enable us to address the key molecular mechanisms underlying the adverse health effects of arsenicals.
Subject(s)
Arsenic , Arsenicals , Proteome , Ecosystem , Arsenicals/chemistry , Biotin/chemistryABSTRACT
Toxic metals can substantially change the bacterial community and functions thereof in aquatic environments. Herein, metal resistance genes (MRGs) are the core genetic foundation for microbial responses to the threats of toxic metals. In this study, waterborne bacteria collected from the Pearl River Estuary (PRE) were separated into the free-living bacteria (FLB) and particle-attached bacteria (PAB), and analyzed using metagenomic approaches. MRGs were ubiquitous in the PRE water and mainly related to Cu, Cr, Zn, Cd and Hg. The levels of PAB MRGs in the PRE water ranged from 8.11 × 109 to 9.93 × 1012 copies/kg, which were significantly higher than those of the FLB (p < 0.01). It could be attributed to a large bacterial population attached on the suspended particulate matters (SPMs), which was evidenced by a significant correlation between the PAB MRGs and 16S rRNA gene levels in the PRE water (p < 0.05). Moreover, the total levels of PAB MRGs were also significantly correlated with those of FLB MRGs in the PRE water. The spatial pattern of MRGs of both FLB and PAB exhibited a declining trend from the low reaches of the PR to the PRE and on to the coastal areas, which was closely related to metal pollution degree. MRGs likely carried by plasmids were also enriched on the SPMs with a range from to 3.85 × 108 to 3.08 × 1012 copies/kg. MRG profiles and taxonomic composition of the predicted MRG hosts were significantly different between the FLB and PAB in the PRE water. Our results suggested that FLB and PAB could behave differential response to heavy metals in the aquatic environments from the perspective of MRGs.
Subject(s)
Metals, Heavy , Rivers , Rivers/microbiology , Estuaries , RNA, Ribosomal, 16S/genetics , Bacteria/genetics , Metals, Heavy/toxicity , Metals, Heavy/analysis , Genes, Bacterial , WaterABSTRACT
Tumor cell migration induced by arsenite (iAsIII) is closely associated with cancer progression. However, transcriptomic and metabolic traits of migrative human cells exposed to iAsIII remain to be well characterized. Here, the combination of transcriptomics and metabolomics approaches were employed to construct interactive networks of functional genes and metabolites in human colorectal cancer (DLD-1) cells exposed to iAsIII. The number of DLD-1 cells passing through the Transwell membrane was at least 6 times greater in the iAsIII-treated groups than in controls. Following iAsIII treatment, the expression of ZEB1 and SLUG protein was significantly upregulated while the expression of CRB2 was downregulated (p < 0.05), indicating the onset of epithelial to mesenchymal transition (EMT). Meanwhile, integrin- and collagen-mediated biological adhesion were enhanced by SLUG under iAsIII treatment. The expression of matrix metallopeptidase (MMP) genes was fostered by iAsIII, which have the functions to degrade extracellular matrix. Glutamine metabolism could be considerably interfered by iAsIII, and in turn glutamine supplementation could effectively enhance DLD-1 cell movement. Overall, our results suggested that DLD-1 cell migration could be promoted by iAsIII via a series of cellular events, including EMT activation, altered cell adhesion, MMP-dependent matrix degradation, accompanying with a metabolic focus on glutamine.
Subject(s)
Arsenites , Colorectal Neoplasms , Humans , Arsenites/toxicity , Epithelial-Mesenchymal Transition/physiology , Glutamine/pharmacology , Cell Movement , Cell Line, Tumor , Colorectal Neoplasms/geneticsABSTRACT
Large cohorts of samples from multiple batches are usually required for global metabolomic studies to characterize the metabolic state of human disease. As such, it is critical to eliminate systematic variation and truly reveal the biologically associated alterations. In this study, we proposed a reference material-based approach (Ref-M) for data correction by liquid chromatography-mass spectrometry and represented by an analysis of multibatch human serum samples. The reference material was generated by mixing serum from healthy donors and distributed to each extraction batch of subject samples. Pooled quality control samples and isotopic internal standards were then applied in each acquisition batch for data quality control. Finally, each metabolite in subject samples was normalized by its counterpart in the reference serum. We demonstrated that Ref-M significantly enhanced the numbers of efficient features and effectively eliminated the batch variation of 522 serum samples of healthy individuals, benign pulmonary nodules, and lung cancer patients. Twenty differential metabolites were identified to distinguish lung cancer from healthy controls in the training set. The discriminant model was validated in an independent data set with an area under the receiver operating characteristics (ROC) curve (AUC) of 0.853. Another 40 serum samples further tested with Ref-M were achieved an AUC of 0.843 by the established model. Our results showed that the reference material-based approach presents the potential to improve the data comparability and precision for biomarker discovery in large-scale metabolomic studies.
Subject(s)
Lung Neoplasms , Tandem Mass Spectrometry , Humans , Chromatography, Liquid/methods , Metabolomics/methods , Metabolome , Lung Neoplasms/metabolismABSTRACT
Variations in the distribution, source composition, mass inventory and burial flux of polycyclic aromatic hydrocarbons (PAHs) in surface sediments from the Pearl River Estuary (PRE) collected in 2011 and 2019 were analyzed to investigate the influence of the anthropogenic activities. Total concentrations of 16 priority PAHs in 2019 (200.40 ± 188.86 ng g-1 dry weight on average) were at the medium level among global bays/estuaries/coastal areas. In 2019, PAH concentrations have decreased by about 50% compared to 2011 and the dominant composition has changed from low- to high-molecular-weight PAHs. The qualitative and quantitative source apportionment analysis indicates that the dominant source of PAHs has shifted from petroleum (40.33%) in 2011 to traffic emission (44.17%) in 2019. The source variation in the PRE can be attributed to the transformation of the energy source structure from petrogenic to pyrogenic in the Pearl River Delta. The estimated PAH mass inventory of the top 5-cm sediment was 38.70 metric tons in 2019, which was about 41 metric tons lower than that in 2011. The average deposition fluxes have dropped from 418.91 ± 261.02 ng cm-2 yr-1 in 2011 to 215.52 ± 246.63 ng cm-2 yr-1 in 2019. The decreasing PAH concentration is related to the sediment coarsening and decline of total organic carbon. These findings in the PRE can be applied to other estuarine environments influenced by anthropogenic activities.
Subject(s)
Polycyclic Aromatic Hydrocarbons , Water Pollutants, Chemical , Rivers/chemistry , Estuaries , Anthropogenic Effects , Geologic Sediments/chemistry , Water Pollutants, Chemical/analysis , Polycyclic Aromatic Hydrocarbons/analysis , Environmental Monitoring , ChinaABSTRACT
Infectious diseases caused by viruses have attracted global concern owing to their rapid spread and catastrophic consequences. Therefore, developing fast and reliable on-site virus detection methods is essential for the prevention and treatment of virus-related diseases. In this study, immunoassays on a membrane, combining virus preconcentration with nanoparticle-based signal amplification, were used to realize the rapid and accurate visual detection of viruses. The biotin-streptavidin scaffolds for target virus preconcentration were established on a membrane, and subsequently a Zika aptamer (Apt) immobilized on the membrane recognized and captured the nonstructural protein 1 of Zika virus (ZIKV-NS1). The probe for detection was synthesized by conjugating the Zika Apt with a high level of horseradish peroxidase on gold nanoparticles. The ZIKV-loaded membrane was incubated with the probes, and the viral signal was amplified as the signal of horseradish peroxidase. In the presence of 3,3,5',5'-tetramethyl benzidine and hydrogen peroxide, the green color of the probe-coated membrane indicated the level of ZIKV-NS1. Our developed method could reach a detection limit of 5 ng mL-1, and the whole procedure could be completed within 1 h. Analyses of rabbit serum and environmental water samples demonstrated that an immunoassay-based approach on the membrane could accurately determine the level of ZIKV-NS1 against the complicated matrix. Our results suggest that this virus detection method has a high potential for application in clinical and environmental settings.
Subject(s)
Metal Nanoparticles , Zika Virus Infection , Zika Virus , Animals , Biotin , Dimaprit/analogs & derivatives , Gold/chemistry , Horseradish Peroxidase , Hydrogen Peroxide , Metal Nanoparticles/chemistry , Rabbits , Streptavidin , Viral Nonstructural Proteins/analysis , Water , Zika Virus/chemistry , Zika Virus Infection/diagnosis , Zika Virus Infection/prevention & controlABSTRACT
The alpine ecosystem as one of the most representative terrestrial ecosystems has been highly concerned due to its susceptibility to anthropogenic impacts and climatic changes. However, the distribution pattern of alpine soil bacterial communities and related deterministic factors still remain to be explored. In this study, soils were collected from different altitudes and slope aspects of the Mount (Mt.) Shergyla, Tibetan Plateau, and were analyzed using 16S rRNA gene-based bioinformatics approaches. Acidobacteriota and Proteobacteria were identified consistently as the two predominant phyla in all soil samples, accounting for approximately 74% of the bacterial community. The alpha diversity of the soil bacterial community generally increased as the vegetation changed with the elevated altitude, but no significant differences in alpha diversity were observed between the two slopes. Beta diversity analysis of bacterial community showed that soil samples from the north slope were always differentiated obviously from the paired samples at the south slope with the same altitude. The whole network constituted by soil bacterial genera at the Mt. Shergyla was parsed into eight modules, and Elev-16S-573, Sericytochromatia, KD4-96, TK10, Pedomicrobium, and IMCC26256 genera were identified as the "hubs" in the largest module. The distance-based redundancy analysis (db-RDA) demonstrated that variations in soil bacterial community thereof with the altitude and slope aspects at the Mt. Shergyla were closely associated with environmental variables such as soil pH, soil water content, metal concentrations, etc. Our results suggest that environmental variables could serve as the deterministic factors for shaping the spatial pattern of soil bacterial community in the alpine ecosystems.
ABSTRACT
To simulate the real cell status and morphology in the living systems is substantial for using cell models to address the detrimental effects of toxic contaminants. In this study, the comparative profiles of metabolites in three-dimensional (3D) human normal liver (L-02) cell spheroids with perfluorooctanoic acid (PFOA) treatment were analyzed using a metabolomic approach. The uniform 3D cell spheroids were well formed in 3 days (e.g., sphericity index >0.9) and stably maintained over the subsequent 11 days. The cytotoxicity of PFOA to the 3D L-02 cell spheroids was highly dependent on both exposure concentration and duration. Comparative analysis of metabolomes showed that the number of differential metabolites in the 3D cell spheroids treated with 300 µM PFOA for 10 days (n = 59) was greater than those with a 4-day exposure to 300 µM PFOA (n = 17). Six metabolic pathways related to amino acids metabolism were only found in the 3D cell spheroids with a 10-day treatment of 300 µM PFOA, which could not be found in the 2D monolayer cells and those 3D cell spheroids with a 4-day exposure. The suppression of PFOA on glutamine metabolism substantially decreased glutathione (GSH) production and accordingly increased the level of reactive oxygen species in the 3D cell spheroids. On the contrary, the supplementation of glutamine increased GSH production and the viability of cell spheroids, indicating that glutamine metabolism played a critical role in the chronic toxic effects of PFOA. Our study strongly suggested that comprehensive toxicological methodologies based on the 3D cell models could currently be robust and suitable for addressing the chronic adverse effects of toxic contaminants.