ABSTRACT
In heterogeneous catalysis, surface defects are widely regarded as an effective means to enhance the catalytic performance of catalysts. In this study, the oxygen vacancy-rich Mg(1-X)ZnXO solid solution support was successfully prepared by doping a small amount of Zn into MgO nanocrystals. Based on this support, Ru/Ba-Mg(1-X)ZnXO catalyst for ammonia synthesis was prepared. Characterization using TEM, EPR, XPS, and DFT calculations confirmed the successful substitution of Zn atoms for Mg atoms leading to the formation of more oxygen vacancies (OVs). N2-TPD, SEM and TEM analyses revealed that a small amount of Zn had minimal influence on the surface morphology and the size of Ru nanoparticles. The abundance of OVs in the support was identified as the primary factor enhancing the catalytic activity. XPS, H2-TPD and kinetics experiment studies further elucidated the mechanism by which OVs promote the reaction, with OVs serving as an anchor point for the promoter Ba on the MgO support and promoted the dispersion of Ba. This anchoring effect not only enhanced the electron density on Ru, favoring the dissociation of the N[triple bond, length as m-dash]N bond, but also mitigated hydrogen poisoning. As a result,the ammonia synthesis rate reached 1.73 mmol g-1 h-1. Furthermore, the CO2-TPD and H2-TPR analyses indicated that Zn doping effectively promotes the metal-support interaction (MSI) and surface alkalinity. The findings of this study offers valuable insights for the design of defective modified catalyst supports.
ABSTRACT
INTRODUCTION: Preeclampsia (PE) is a severe obstetric complication closely associated with placental dysfunction. Placental mesenchymal stem/stromal cells (PMSCs) modulate placental development while PE PMSCs are excessively senescent to disturb placental function. Nevertheless, the senescence mechanism of PE PMSCs remains unclear. METHODS: PE-related single-cell RNA sequencing datasets (GSE173193), data of chip detection (GSE99007) and bulk transcriptome RNA sequencing datasets (GSE75010) were extracted from the GEO database. Firstly, the functional enrichment analyses of the differentially expressed genes (DEGs) in PMSCs were conducted. Then, the clusters of PE PMSCs were distinguished according to the expressions of senescence-related genes (SRGs) by consensus clustering analysis. Cell cycle analysis, senescence ß-galactosidase, Transwell, and tube formation were conducted. Next, the expressions of the senescence-associated secretory phenotype (SASPs) were displayed. The characteristic genes of PE were screened by the least absolute shrinkage and selection operator analysis. CTSZ was suppressed in PMSCs and the cellular senescence levels were evaluated. RESULTS: In this study, The DEGs in PMSCs were closely associated with cellular senescence. The score of SRGs was significantly higher and most of the SASPs were abnormally expressed in the senescent group. Seven characteristic genes of PE were identified, thereinto, CTSZ reduction may accelerate the senescence in PMSCs in vitro. DISCUSSION: Combined with bioinformatic analysis and lab experiments, our study emphatically revealed the abnormal cellular senescence in PE PMSCs, in which CTSZ, one of the PE characteristic genes, regulated the cellular senescence levels in PMSCs. These findings might help to deepen the understanding of the senescence mechanism of PMSCs in PE.
Subject(s)
Cellular Senescence , Mesenchymal Stem Cells , Placenta , Pre-Eclampsia , Humans , Female , Cellular Senescence/genetics , Mesenchymal Stem Cells/metabolism , Pre-Eclampsia/genetics , Pre-Eclampsia/metabolism , Pregnancy , Placenta/metabolism , Placenta/pathology , Single-Cell Analysis , Sequence Analysis, RNA , AdultABSTRACT
A new indolizinium alkaloid, named as cyclizidine J (1), was identified from Gause's liquid fermentation of marine-derived Streptomyces sp. HNA39. Its structure was elucidated by extensive NMR spectroscopic methods, HRESIMS data, and ECD calculations. To our best knowledge, compound 1 was a unique cyclizidine-type alkaloid that contain a chlorine atom substituted at position C-8. Unfortunately, biological evaluation of 1 exhibited no active against PC-3 cancer cell line, BRD4, and ROCK2 protein kinase.
Subject(s)
Alkaloids , Streptomyces , Alkaloids/pharmacology , Cell Line, Tumor , Molecular Structure , Nuclear Proteins , Transcription FactorsABSTRACT
BACKGROUND: Adequate caring behaviours provided by nurses can increase patients' satisfaction of care. Yet few researchers have examined women's satisfaction in nurses' caring behaviours in China and then published the study in English. AIM: The aim of this study was to identify the level of women's satisfaction with nurses' caring behaviours during the antenatal, childbirth and postnatal periods. METHODS: A descriptive, cross-sectional study was performed using a sample of 422 postpartum women of two teaching hospitals in Wuhan, China. A structured questionnaire on socio-demographic information was completed, and a satisfaction scale of 60 items concerning nurses' caring behaviours was measured. RESULTS: The findings showed that participants reported a good satisfaction score for nurses' caring behaviours during all three periods. Behaviours on the 'Monitor' subscale obtained the highest score (a mean score of 4.46 for the antenatal period, 4.64 for the childbirth period and 4.31 for the postnatal period). The subscale with the second highest score was 'Human respect' (4.53, 4.27 and 4.56, respectively), while the subscale with the lowest score was 'Trust' (3.78 and 3.98). The statistical significances of the demographics of the women were shown concerning their perceived satisfaction of five subscales of caring behaviours. CONCLUSION: The study revealed no statistically significant differences in the perceived satisfaction in nurses' caring behaviours between the antenatal, childbirth and postnatal periods. However, nursing administration development projects that focus on caring are still needed. Additionally, more studies that reflect Watson's theory of human caring on the maternity population should be conducted with a larger sample size.
Subject(s)
Hospitals, Teaching/organization & administration , Nursing Staff, Hospital/standards , Patient Satisfaction , Adolescent , Adult , China , Cross-Sectional Studies , Female , Humans , Middle Aged , Nursing Staff, Hospital/psychology , Pregnancy , Surveys and Questionnaires , Young AdultABSTRACT
The present paper reported a hybrid structure for the optical recognition of PA (picric acid). This dye-MOF structure, named as R6h@EuBTC, consisted of a supporting matrix based on rare earth MOF and a sensing probe based on rhodamine dye, which was confirmed using XRD, IR, thermal and photophysical analysis. R6h@EuBTC's rhodamine absorption in visible region was enhanced by increasing PA concentrations, showing obvious color change and consequently colorimetric sensing. R6h@EuBTC's rhodamine emission component was increased by increasing PA concentrations, while its Eu emission component was slightly quenched by increasing PA concentrations, which offered self-calibrated sensing signals for ratiometric fluorescent sensing. Linear response and good selectivity were observed for both sensing channels with LOD of 3.9⯵M. R6h@EuBTC's sensing mechanism towards PA was the combination of two procedures, which were the emission turn on effect of rhodamine component triggered by PA-released protons and the emission turn off effect of Eu component caused by its electron transfer procedure to PA, respectively. R6h@EuBTC's novelty was its two sensing channels and the practicability of naked eye detection.
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Long non-coding RNAs are dysregulated in human hepatocellular carcinoma (HCC). We tested the potential effect of long non-coding RNA 00312 ("Lnc00312") on human HCC cell behavior in vitro and in vivo. Forced-expression of Lnc00312 by a lentiviral vector induced proliferation inhibition and apoptosis in HepG2 cells and primary human HCC cells. Lnc00312 downregulated cyclin B1 and induced G2-M cell cycle arrest in HCC cells. Restoring cyclin B1 expression by a cyclin B1 cDNA construct inhibited Lnc00312-induced cytotoxicity against HCC cells. Conversely, siRNA-mediated knockdown of Lnc00312 increased cyclin B1 expression and promoted HepG2 cell proliferation. In vivo, the growth of HepG2 xenograft tumors in severe combined immunodeficient (SCID) mice was largely inhibited after expression of Lnc00312. Significantly, Lnc00312 is downregulated in human HCC tissues, which is negatively correlated with the tumor grade. Overall, Lnc00312 inhibits human HCC cell proliferation in vitro and in vivo. Cyclin B1 could be a key target protein of Lnc00312 in human HCC cells.
Subject(s)
Carcinoma, Hepatocellular/metabolism , Carcinoma, Hepatocellular/pathology , Cell Proliferation , Cyclin B/metabolism , Liver Neoplasms/metabolism , Liver Neoplasms/pathology , RNA, Long Noncoding/metabolism , Animals , Down-Regulation , Female , Gene Expression Regulation, Neoplastic , Hep G2 Cells , Humans , Male , Mice , Mice, SCID , Neoplasm Invasiveness , Tumor Cells, CulturedABSTRACT
Tuberculosis (TB) remains one of the most widespread and leading deadliest diseases, around one-third of the world's population harbor a latent infection by Mycobacterium tuberculosis (MTB), and 5-10% eventually develop an active TB. The emergency of MTB new virulent forms as well as the co-infection between MTB and HIV alarming the serious problem in TB control and demanding the need for new drugs more potent than earlier with safe ADME profile. Fluoroquinolones are emerged as a large family of synthetic broad spectrum antibiotics, and some of them were recommended as the second-line agents for the treatment of TB mainly in cases involving resistance or intolerance to first-line anti-TB therapy by WHO. Numerous of FQs derivatives have been synthesized for seeking for new anti-TB agents, and some of them exhibited promising potency. This review aims to summarize the recent advances made towards the discovery of FQs derivatives as anti-TB agents and the structure-activity relationship of these derivatives.
Subject(s)
Antitubercular Agents/pharmacology , Fluoroquinolones/pharmacology , Mycobacterium tuberculosis/drug effects , Tuberculosis/drug therapy , Animals , Antitubercular Agents/chemical synthesis , Antitubercular Agents/chemistry , Dose-Response Relationship, Drug , Fluoroquinolones/chemical synthesis , Fluoroquinolones/chemistry , Humans , Molecular Structure , Structure-Activity RelationshipABSTRACT
Malaria remains one of the most deadly infectious diseases globally. Considering the growing spread of resistance, development of new and effective antimalarials remains an urgent priority. Quinolones, which are emerged as one of the most important class of antibiotics in the treatment of various bacterial infections, showed potential in vitro antiplasmodial and in vivo antimalarial activities, making them promising candidates for the chemoprophylaxis and treatment of malaria. This review presents the current progresses and applications of quinolone-based derivatives as potential antimalarials to pave the way for the development of new antimalarials.
Subject(s)
Antimalarials/pharmacology , Plasmodium/drug effects , Quinolones/pharmacology , Animals , Antimalarials/chemistry , Humans , Molecular Structure , Parasitic Sensitivity Tests , Quinolones/chemistryABSTRACT
One new indolocarbazole alkaloid, 12-N-methyl-k252c, together with eight known indolocarbazoles were isolated from the rice solid fermentation of the marine-derived Streptomyces sp. A22. Their structures were elucidated on the basis of spectroscopic methods (UV, IR, HRESITOF MS, 1D NMR and 2D NMR). All of these compounds were evaluated for bromodomain-containing protein 4 (BRD4) inhibitory activities and cytotoxic activity assay, respectively. Compounds 4 and 5 showed moderate cytotoxic activity with an IC50 value of 3.52 and 3.93 µM, respectively. Additionally, compound 1 also was tested for enzyme inhibition activities of protein kinases and showed moderate activity with IC50 values of 0.91-1.84 µM.
Subject(s)
Alkaloids/pharmacology , Carbazoles/pharmacology , Seawater/microbiology , Streptomyces/chemistry , Alkaloids/isolation & purification , Antineoplastic Agents/isolation & purification , Antineoplastic Agents/pharmacology , Carbazoles/isolation & purification , Cell Cycle Proteins , Cell Line, Tumor , China , Fermentation , Humans , Magnetic Resonance Spectroscopy , Molecular Structure , Nuclear Proteins/antagonists & inhibitors , Transcription Factors/antagonists & inhibitorsABSTRACT
A fermentation of marine fungus Penicillium janthinellum DT-F29 on solid rice medium led to the isolation of three new compounds, brefeldin D (1) and penialidins D-E (5-6), along with other five known brefeldins and penialidins. The structures of above compounds were determined on the basis of MS and NMR analysis.
Subject(s)
Penicillium/chemistry , Polyketides/chemistry , Anti-HIV Agents/chemistry , Anti-HIV Agents/pharmacology , Aquatic Organisms , Culture Media , Fermentation , Macrolides/chemistry , Magnetic Resonance Spectroscopy , Molecular Structure , Oryza , Penicillium/growth & developmentABSTRACT
OBJECTIVE: Effective treatments for preeclampsia are currently unavailable. As endothelial progenitor cell-transplantation may improve ischemia, it is an important undertaking to study the role of endothelial progenitor cells for improving the symptom of preeclampsia. METHOD: Physiological and pathological changes in foetal rats and pregnant rats were monitored. Endothelial progenitor cells were isolated from the peripheral blood of normal rats and labelled by DiI. Endothelial progenitor cells were transplanted into the placenta of preeclampsia-like rats. Fluorescence microscopy was used to observe the differentiation of transplanted endothelial progenitor cells. Western blotting was used to observe the expression of nestin, an index of brain hypoxia in foetal rats. RESULT: The rats suffered from abdominal aortic constriction and NG-nitro-L-arginine methyl ester injection (group F). The proteinuria and blood pressure of pregnant rats in group F increased on the 13th day of pregnancy. The proteinuria and blood pressure of group F was higher than in other groups of rats. The weight of foetal rats and foetal heads significantly decreased in group F compared with other groups. Typical pathological changes of preeclampsia were observed in the placental tissue of group F. In preeclampsia-like rats, transplantation of endothelial progenitor cells led to an increase in placenta angiogenesis. The expression of nestin weakened in endothelial progenitor cell-transplanted rats compared with the non-transplantation group. After EPCs transplantation, physiological parameters in the preeclampsia-like rats were significantly decreased. CONCLUSION: Endothelial progenitor cells transplantation could improve preeclampsia-like symptom in rats and endothelial progenitor cell-transplantation relieves intrauterine hypoxia in brain tissues of foetal rats to a certain extent.
Subject(s)
Endothelial Progenitor Cells/transplantation , Pre-Eclampsia/surgery , Stem Cell Transplantation , Animals , Blood Pressure/physiology , Cell Differentiation , Female , Fetus/pathology , Humans , Male , Placenta/metabolism , Pre-Eclampsia/physiopathology , Pregnancy , Proteinuria , RatsABSTRACT
OBJECTIVE: Based on a retrospective analysis of the drunk driving cases, to explore the drunk drivers' personnel composition, occurrence time and psychology. METHODS: As a result of punishment of the drunk driving by criminal law for one year from May 1st, 2011 to April 30th, 2012, 91 drunk driving cases were statistically analyzed the easy-happening time of drunk driving, the drunk drivers' age, gender, occupational characteristics, domicile and psychological factors. RESULTS: In 97 drunk driving cases, 26-40 years old, non-local domiciled and non-professional male drivers were prone to drunk driving at night from 22:00 to 5:00. CONCLUSION: The behavior of drunk driving is relevant to time, age, genders and occupation. The psychological characteristics of most drivers are fluky, making-life-easy, competitive and peacockish.
Subject(s)
Accidents, Traffic/statistics & numerical data , Alcohol Drinking , Alcoholic Intoxication/epidemiology , Automobile Driving/legislation & jurisprudence , Adolescent , Adult , Age Distribution , Alcoholic Intoxication/psychology , Automobile Driving/psychology , China/epidemiology , Female , Forensic Medicine , Humans , Male , Middle Aged , Risk Factors , Sex Distribution , Social Behavior , Time Factors , Young AdultABSTRACT
Abstract: Suxamethonium chloride is a depolarizing muscle relaxant used in general anesthesia. In overdose, it causes adverse reactions such as bradycardia, arrhythmia, cardiac arrest, and death. The article reviews the progress on testing methods of suxamethonium chloride such as infrared spectroscopy, chemical color reaction, chemical titration, enzyme electrode, chromatography and mass spectrometry.
Subject(s)
Anesthesia, General , Arrhythmias, Cardiac/chemically induced , Bradycardia/chemically induced , Heart Arrest/chemically induced , Neuromuscular Depolarizing Agents/adverse effects , Succinylcholine/adverse effects , Biosensing Techniques , Chromatography , Drug Overdose , Humans , Mass Spectrometry , Neuromuscular Depolarizing Agents/analysis , Spectrophotometry, Infrared , Succinylcholine/analysisABSTRACT
Micellar liquid chromatography (MLC) is a reversed phase liquid chromatography with mobile phases containing surfactant above its critical micellar concentration (CMC). The basic mechanism and advantages of MLC in physicochemical analysis were reviewed, and its applications in analysis of drugs, barbiturates, benzodiazepines were chiefly introduced in this paper. MLC is a potential method to toxicological analysis due to strong selectivity, wide application scope and easy biological samples, etc.
Subject(s)
Analgesics, Opioid/analysis , Barbiturates/analysis , Benzodiazepines/analysis , Chromatography, Liquid/methods , Hypnotics and Sedatives/analysis , Barbiturates/chemistry , Benzodiazepines/chemistry , Humans , Hypnotics and Sedatives/chemistry , Micelles , Reproducibility of Results , Sensitivity and Specificity , Solvents/chemistry , Surface-Active Agents/chemistryABSTRACT
The mol-ecule of the title compound, C(10)H(12)N(2)O(3), adopts a trans configuration with respect to the C=N bond. The dihedral angle between the benzene ring and the hydrazinecarboxyl-ate plane is 8.98â (7)°. Intra-molecular O-Hâ¯N and C-Hâ¯N hydrogen bonds are observed. Mol-ecules are linked into chains along the c axis by N-Hâ¯O hydrogen bonds. In addition, C-Hâ¯π inter-actions are observed.
ABSTRACT
In the title compound, C(15)H(20)ClNO(3), the seven-membered ring adopts a distorted boat-sofa conformation; the methyl-ene C atoms of this ring are coplanar with the benzene ring. Both meth-oxy groups are almost coplanar with the attached benzene ring [C-C-O-C = 6.5â (2) and -13.5â (3)°]. An intra-molecular C-Hâ¯O hydrogen bond is observed in the mol-ecular structure. In the crystal structure, a C-Hâ¯π inter-action involving the benzene ring is observed.
ABSTRACT
In the title compound, C(15)H(18)ClNO(3), the seven-membered ring has a mirror plane passing through the methyl-ene C atom and bis-ecting the C=C bond. It adopts a bent conformation, inter-mediate between the boat and chair forms. Both meth-oxy groups are coplanar with the attached benzene ring [C-C-O-C = -0.5â (3) and 2.2â (3)°]. In the crystal structure, inversion-related mol-ecules are linked via C-Hâ¯O hydrogen bonds and π-π inter-actions involving the benzene ring [centroid-centroid distance = 3.6393â (12)Å].
ABSTRACT
IN THE TITLE COMPOUND [SYSTEMATIC NAME: 2-(1-adamantyl)-4-bromo-1--methoxy-benzene], C(17)H(21)BrO, two weak intra-molecular C-Hâ¯O hydrogen bonds influence the mol-ecular conformation. The crystal packing exhibits C-Hâ¯π inter-actions, with a relatively short inter-molecular Câ¯Cg contact of 3.568â (5)â Å, where Cg is the centroid of the benzene ring. The crystal studied exhibited inversion twinning.
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In the title compound, C(9)H(10)N(2)O(3), the hydroxy group and the C=N-N unit are coplanar with the benzene ring. The benzene rings of inversion-related mol-ecules are stacked with their centroids separated by a distance of 3.7703â (9)â Å, indicating weak π-π inter-actions. In the crystal structure, C-Hâ¯O, O-Hâ¯O, N-Hâ¯O and C-Hâ¯O hydrogen bonds link molecules into a infinite two-dimensional network along the a axis.
ABSTRACT
In the title compound, C(12)H(15)NO(3), all C, N and O atoms lie in a mirror plane. An intramolecular C-Hâ¯O hydrogen bond is present.