Subject(s)
Gram-Negative Bacteria/genetics , Gram-Positive Bacteria/genetics , Polymerase Chain Reaction/methods , Adult , Cystic Fibrosis/microbiology , Databases, Nucleic Acid , Hematologic Neoplasms/microbiology , Humans , Nontuberculous Mycobacteria/genetics , Phenotype , Pseudomonas aeruginosa/genetics , Sequence Analysis, DNA/methods , Sequence Analysis, RNA/methods , Sputum/microbiologySubject(s)
Bacteria/isolation & purification , Bacterial Infections/veterinary , Camelus , Feces/microbiology , Animals , Animals, Suckling , Bacteria/drug effects , Bacterial Infections/epidemiology , Campylobacter Infections/epidemiology , Campylobacter Infections/veterinary , Dysentery, Bacillary/epidemiology , Dysentery, Bacillary/veterinary , Escherichia coli Infections/epidemiology , Escherichia coli Infections/veterinary , Female , Male , Microbial Sensitivity Tests/veterinary , Prevalence , Salmonella Infections, Animal/epidemiology , United Arab Emirates/epidemiology , Yersinia Infections/epidemiology , Yersinia Infections/veterinaryABSTRACT
BACKGROUND: Cryptosporidium parvum is the most common of the protozoal pathogens associated with gastrointestinal disease in Northern Ireland. Genotyping techniques are valuable in helping to elucidate sources and modes of transmission of this parasite. There have been no reports on the prevalence of genotypes in Northern Ireland, mainly due to a lack of discriminatory genotyping techniques, which recently have become available. AIM: To investigate the genotype of C. parvum oocysts isolated from human faeces in sporadic cases of cryptosporidiosis in Northern Ireland. METHODS: Thirty-nine isolates of C. parvum, representing 79.6% of the total 1998 laboratory reports for the Eastern Health and Social Services Board, were investigated. Following DNA extraction from oocysts the thrombospondin-related adhesive protein 2 (TRAP-C2) locus was amplified by polymerase chain reaction (PCR) and subsequently sequenced. RESULTS: The majority of isolates (87.2%) were classified as bovine genotype II with the remainder (12.8%) being the human genotype I. CONCLUSIONS: There is a high prevalence of the bovine genotype II parasite in sporadic cases around the greater Belfast area. Epidemiologically, this suggests that the most frequent mode of transmission may be from animals to humans, but does not suggest a high proportion of human to human spread.
Subject(s)
Cryptosporidiosis/epidemiology , Cryptosporidium parvum/genetics , Polymorphism, Genetic , Adolescent , Adult , Animals , Child , Child, Preschool , Feces/parasitology , Female , Genotype , Humans , Infant , Male , Middle Aged , Molecular Epidemiology , Northern Ireland/epidemiology , Plasmodium/genetics , Polymerase Chain Reaction , Protozoan Proteins/geneticsABSTRACT
Terms such as race, sex and age are assumed to reflect biological characteristics and distinctions. In psychological research, these terms are often treated as if they were a reflection of a meaningful set of psychological constructs. A review of articles in 3 prominent journals over a 30-year period reveals that these supposed biological identifiers are not used consistently and lack empirical and conceptual validity. An analysis of those articles shows that, over time, the term race has given way to the use of the more general and psychologically relevant term ethnicity, sex and gender have been used interchangeably, and the psychological constructs underlying or supposedly reflected in age are seldom discussed. It is proposed that psychosocial researchers and editors adopt a consistent definition of these terms and that research include an effort to identify the underlying concepts that the investigators assume to be reflected in these distinctions whenever these labels are used to report research findings.
