ABSTRACT
Soft materials such as biological tissues are prone to deformation and generate different stable structures under external stimulation. This property is widely used to create tunable patterns, and the tuning of the wrinkling patterns can be applied to the control of elastic waves. In this paper, the wrinkling modes of film/substrate systems with different geometric dimensions and material parameters were studied. It is verified by numerical simulation that the elastic wave band gaps corresponding to the two wrinkling modes can be effectively superposed in one system, and the experimental samples with two wrinkling modes coexisting in one system were prepared by parameter optimization and a moisture-curing process. A vibration test showed that the hybrid system could effectively suppress the propagation of elastic waves. Combined with engineering needs, the wrinkling system under different loading conditions was studied, which provides a design guide for widening and regulating the elastic wave band gap.
ABSTRACT
KEY MESSAGE: Down-regulation of starch branching enzymes alters fine structure and starch properties, especially the B-type crystalline pattern and extremely high amylose content identified in the BEIIb-deficiency mutant in the indica rice. The relative importance of the starch branching enzymes in determining the molecular fine structure and starch functional properties were uncovered in this study. An indica rice, Guangluai 4 with high amylose content (AC) and high gelatinization temperature (GT) was used to generate the clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein-9 (Cas9) knockout lines. Five mutant lines were identified including be1-1, be1-2, be2a-1, be2a-2 and be2b-1, and analysis of western blot showed the CRISPR/Cas9 system was successful in inducing mutations in the targeted genes. AC of be2b-1 (34.1%) was greater than that of wild type (WT) (27.4%) and other mutants. Mutations of either BEI or BEIIa did not alter the starch crystallite pattern (A-type). The BEIIb deficiency caused an opaque endosperm phenotype, changed the crystallite pattern from A- to B-type, and dramatically increased the degree of ordered structure, the relative proportion of amylose chains and intermediate to long amylopectin chains, average chain length of amylopectin molecules as well as GT. The BEIIa deficiency had no effect on the proportion of amylose chains, the length of amylopectin intermediate-long chains, conclusion temperature and enthalpy of gelatinization. Down-regulation of BEI increased the proportion of shortest amylopectin chains (fa) but decreased the proportion of long amylopectin chains (fb2 and fb3), leading to a lower GT. It is concluded that the relative importance in determining starch fine structures and functionality was in the order of BEIIb > BEI > BEIIa. Our results provide new information for utilizations of BE-deficient mutants in rice quality breeding.