ABSTRACT
This review focuses on changes in nutrition and functional properties of protein-rich foods, primarily attributed to alterations in protein structures. We provide a comprehensive overview and comparison of commonly used laboratory methods for protein structure identification, aiming to offer readers a convenient understanding of these techniques. The review covers a range of detection technologies employed in food protein analysis and conducts an extensive comparison to identify the most suitable method for various proteins. While these techniques offer distinct advantages for protein structure determination, the inherent complexity of food matrices presents ongoing challenges. Further research is necessary to develop and enhance more robust detection methods to improve accuracy in protein conformation and structure analysis.
ABSTRACT
The use of soy protein isolate (SPI) nanoparticles as a stabilizer in nano-emulsion systems has garnered significant interest. While metal-phenolic networks (MPNs) have been explored for their multifunctional surface modification capabilities, their integration with food protein-based delivery systems remains less explored. In this study, we attempt to develop a novel strategy to encapsulate cinnamaldehyde using MPNs (EGCG-Fe3+) with self-assembling soy protein nanoparticles (SE-Fe NPs) as a stabilizer for nano-emulsions. UV, Raman, and X-ray photoelectron spectroscopy analyses demonstrated that SE-Fe NPs were generated through metal-phenolic coordination and covalent interactions. SE-Fe NPs had a narrower particle size distribution and enhanced radical scavenging (up to 3.35-fold), as well as thermal stability. Furthermore, the smaller droplet size, higher modulus, higher cinnamaldehyde encapsulation efficiency (from 63.5% to 84.2%), and improved bio-accessibility of SE-Fe NPs stabilized nano-emulsions delivery system demonstrated in this study shows promising future applications in the food industry.
ABSTRACT
Electrospun films (ESF) are gaining attention for active delivery due to their biocompatibility and biodegradability. This study investigated the impact of adding soy protein amyloid fibrils (SAFs) to ESF. Functional ESF based on SAFs/pullulan were successfully fabricated, with SAFs clearly observed entangled in the electrospun fibers using fluorescence microscopy. The addition of SAFs improved the mechanical strength of the ESF threefold and increased its surface hydrophobicity from 24.8° to 49.9°. Moreover, the ESF demonstrated antibacterial properties against Escherichia coli and Staphylococcus aureus. In simulated oral disintegration tests, almost 100% of epigallocatechin gallate (EGCG) dissolved within 4 min from the ESF. In summary, the incorporation of SAFs into ESF improved their mechanical strength, hydrophobicity, and enabled them to exhibit antibacterial properties, making them promising candidates for active delivery applications in food systems. Additionally, the ESF showed efficient release of EGCG, indicating their potential for controlled release of bioactive compounds.
ABSTRACT
The hierarchically porous property of CaCO3 has attracted considerable attention in the field of active delivery ingredients due to its high adsorption capacity. Here, a facile and high-efficient approach to control the calcification processes of CaCO3 ending with calcite microparticles with superior porosity and stability is reported and evaluated. In this work, a series of quercetin promoted CaCO3 microparticles, using soy protein isolate (SPI) as entrapment agent, was synthesized, characterized, and their digestive behavior and antibacterial activity were evaluated. Results obtained indicated that quercetin showed good ability to direct the calcification pathway of amorphous calcium carbonate (ACC) with the formation of flower- and petal-like structures. The quercetin-loaded CaCO3 microparticles (QCM) had a macro-meso-micropore structure, which was identified to be the calcite form. The macro-meso-micropore structure provided QCM with the largest surface area of 78.984 m2g-1. The loading ratio of SPI to QCM was up to 200.94 µg per mg of QCM. The protein and quercetin composite microparticles (PQM) were produced by simply dissolving the CaCO3 core, and the obtained PQM was used for the delivery of quercetin and protein. Thermogravimetric analysis showed PQM presented with good thermal stability without the CaCO3 core. Furthermore, minor discrepancy was noted in protein conformational structures after removing the CaCO3 core. In vitro digestion revealed that approximately 80% of the loaded quercetin was released from PQM during intestinal digestion, and the released quercetin exhibited efficient transportation across the Caco-2 cell monolayer. More importantly, the PQM digesta retained enhanced antibacterial activities to inhibit growth of Escherichia coli and Staphylococcus aureus. Porous calcites show a high potential as a delivery system for food applications.
Subject(s)
Calcium Carbonate , Quercetin , Humans , Porosity , Quercetin/pharmacology , Calcium Carbonate/chemistry , Caco-2 Cells , Anti-Bacterial Agents/pharmacology , Proteins , Escherichia coliABSTRACT
Effective training is crucial for patients who need rehabilitation for achieving optimal recovery and reducing complications. Herein, a wireless rehabilitation training monitoring band with a highly sensitive pressure sensor is proposed and designed. It utilizes polyaniline@waterborne polyurethane (PANI@WPU) as a piezoresistive composite material, which is prepared via the in situ grafting polymerization of PANI on the WPU surface. WPU is designed and synthesized with tunable glass transition temperatures ranging from -60 to 0 °C. Dipentaerythritol (Di-PE) and ureidopyrimidinone (UPy) groups are introduced, endowing the material with good tensile strength (14.2 MPa), toughness (62 MJ-1 m-3 ), and great elasticity (low permanent deformation: 2%). Di-PE and UPy enhance the mechanical properties of WPU by increasing the cross-linking density and crystallinity. Combining the toughness of WPU and the high-density microstructure derived by hot embossing technology, the pressure sensor exhibits high sensitivity (168.1 kPa-1 ), fast response time (32 ms), and excellent stability (10 000 cycles with 3.5% decay). In addition, the rehabilitation training monitoring band is equipped with a wireless Bluetooth module, which can be easily applied to monitor the rehabilitation training effect of patients using an applet. Therefore, this work has the potential to significantly broaden the application of WPU-based pressure sensors for rehabilitation monitoring.
ABSTRACT
Soy protein-derived amyloid fibrils (SAFs) held desirable features, and with rational tailoring of physical structures, their techno-functions could be further improved. Here, we report a strategy for tailoring SAFs to form hydrogels with appealing mechanical properties as mediated by (-)-epigallocatechin-3-gallate (EGCG). The SAFs-EGCG complexes are characterized by measuring changes in gelling properties, identifying interfacing residues, and understanding the molecular geometry of complexes. EGCG is found to cleave rigid SAFs and induce the formation of large branched chains, which are essential for forming gel-like structures. Results in this study show that SAFs-EGCG complexes and their digesta are non-toxic in human cell lines, and these complexes are superior in inhibiting the growth of Escherichia coli and Staphylococcus aureus. This study provides new insights into remodeling structures and steering techno-functions of SAFs through interaction with EGCG, and will serve as a basis for EGCG as a potent remodeling agent of food protein-derived fibrils.
Subject(s)
Amyloid , Catechin , Amyloid/chemistry , Catechin/analogs & derivatives , Catechin/chemistry , Humans , Soybean Proteins/pharmacologyABSTRACT
Herein, we propose a highly sensitive wireless rehabilitation training ball with a piezoresistive sensor array for patients with Parkinson's disease (PD). The piezoresistive material is a low percolation threshold conductive hydrogel which is formed with polypyrrole (PPy) nanofibers (NFs) as a conductive filler derived from a polydopamine (PDA) template. The proton acid doping effect and molecular template of PDA are essential for endowing PPy NFs with a high aspect ratio, leading to a low percolation threshold (â¼0.78 vol %) and a low Young's 004Dodulus of 37.69 kPa and hence easy deformation. The piezoresistive sensor exhibited a static and dynamic stability of 10,000 s and 15,000 cycle times, respectively. This stability could be attributed to the increased hydrophilicity of conductive fillers, enhancing the interfacial strength between the conductive filler and the matrix. The interaction between the PDA-PPy NFs and the hydrogel matrix endows the hydrogel with toughness and ensures the stability of the device. Additionally, the microdome structure of the conductive hydrogel, produced by hot screen-imprinting, dramatically improves the sensitivity of the piezoresistive sensor (â¼856.14 kPa-1). The microdome conductive hydrogel can distinguish a subtle pressure of 15.40 Pa compared to the control hydrogel without a microstructure. The highly sensitive piezoresistive sensor has the potential to monitor the hand-grip force, which is not well controlled by patients with PD. The rehabilitation training ball assembled with a sensor array on the surface and a wireless chip for communication inside is built and used to monitor the pressure in real time through the WeChat applet. Thus, this work has significantly broadened the application of hydrogel-based flexible piezoresistive sensors for human activity monitoring, which provides a promising strategy to realize next-generation electronics.
Subject(s)
Nanofibers , Polymers , Electric Conductivity , Humans , Hydrogels/chemistry , PyrrolesABSTRACT
Traditional supervised time series classification (TSC) tasks assume that all training data are labeled. However, in practice, manually labelling all unlabeled data could be very time-consuming and often requires the participation of skilled domain experts. In this paper, we concern with the positive unlabeled time series classification problem (PUTSC), which refers to automatically labelling the large unlabeled set U based on a small positive labeled set PL. The self-training (ST) is the most widely used method for solving the PUTSC problem and has attracted increased attention due to its simplicity and effectiveness. The existing ST methods simply employ the one-nearest-neighbor (1NN) formula to determine which unlabeled time-series should be labeled. Nevertheless, we note that the 1NN formula might not be optimal for PUTSC tasks because it may be sensitive to the initial labeled data located near the boundary between the positive and negative classes. To overcome this issue, in this paper we propose an exploratory methodology called ST-average. Unlike conventional ST-based approaches, ST-average utilizes the average sequence calculated by DTW barycenter averaging technique to label the data. Compared with any individuals in PL set, the average sequence is more representative. Our proposal is insensitive to the initial labeled data and is more reliable than existing ST-based methods. Besides, we demonstrate that ST-average can naturally be implemented along with many existing techniques used in original ST. Experimental results on public datasets show that ST-average performs better than related popular methods.
Subject(s)
Cluster Analysis , HumansABSTRACT
Natural soy oleosomes are known to have a remarkable stability, given the advantage of their sophisticated membrane. The aim of the present study is to examine the concept of fabricating a ß-carotene emulsion stabilized by soy oleosin (OLE) and lecithin (LEC) mixtures mimicking the membrane composition of soy oleosomes while providing preferable stability and bioaccessibility. For this, the fabricated emulsion was characterized in terms of droplet size distribution, and emulsion structure, stability and digestion (release and absorption of lipophilic ß-carotene). Compared to SPI/LEC (10 : 1) stabilized emulsions, the OLE/LEC (10 : 1) mixture stabilized emulsion exhibited the highest emulsifying activity index (EAI) and emulsifying stability index (ESI) values, and higher encapsulation efficiency. Results show that the ß-carotene emulsion stabilized by OLE and LEC mixtures at the ratio of 10 : 1 (w/w) has the most uniform droplet distribution and highest stability. The in vitro gastrointestinal digestion test revealed that the ß-carotene emulsion stabilized by OLE and LEC mixtures was digested more rapidly than the emulsion stabilized by soy protein isolate (SPI) and LEC mixtures. In turn, the bioaccessibility and cellular uptake of ß-carotene were enhanced, resulting in a higher absorption, a desirable feature of nutrition delivery systems. Our results demonstrated a promising way to fabricate emulsions mimicking natural soy oleosomes.
Subject(s)
Lecithins/chemistry , Soybean Proteins/chemistry , beta Carotene/pharmacology , Caco-2 Cells , Cell Survival/drug effects , Drug Compounding , Emulsions/chemistry , Humans , beta Carotene/metabolismABSTRACT
In modern cars, the Passive Keyless Entry and Start system (PKES) has been extensively installed. The PKES enables drivers to unlock and start their cars without user interaction. However, it is vulnerable to relay attacks. In this paper, we propose a secure smartphone-type PKES system model based on user context detection. The proposed system uses the barometer and accelerometer embedded in smartphones to detect user context, including human activity and door closing event. These two types of events detection can be used by the PKES to determine the car owner's position when the car receives an unlocking or a start command. We evaluated the performance of the proposed method using a dataset collected from user activity and 1526 door closing events. The results reveal that the proposed method can accurately and effectively detect user activities and door closing events. Therefore, smartphone-type PKES can prevent relay attacks. Furthermore, we tested the detection of door closing event under multiple environmental settings to demonstrate the robustness of the proposed method.
ABSTRACT
Peroxpolytungstic acid (PPTA) is one of the important precursors to synthesize nanostructured tungsten oxides with the chemical routes. In this paperï¼PPTA sol, being prepared with H2O2, W and C2H5OH, was placed at room temperature for a long time till it was jellified naturally and then was dried at 120 â for 3 hours for this investigation. Structures, thermal stability and optical UV-Vis absorption of the samples have been investigated by XRD, SEM, Raman, TG/DSC and UV-Vis spectrum, respectively. The wide peaks from XRD indicate that the sample is amorphous. The Gaussian fitting for these XRD peaks indicates that the sample is the composite of tungsten oxides and tungsten oxide hydrates. SEM indicates that the sample possesses the morphologies of the nano-particles with the sizes of about 50~100 nm and the nano-flakes with the thickness of about 50 nm. The Gaussian fitting of the wide Raman peaks illustrates that the sample possesses the obvious the modes of symmetrical OWO stretching, asymmetrical OWO stretching and WO vibration accompanied with the modes of symmetrical OWO bending, asymmetrical OWO bending and the adsorptive water vibration. This fact further indicates that the sample is composed of the amorphous tungsten oxides and tungsten oxide hydrates. Analysis on the TG/DSC curves indicates that PPTA gels possess four different thermal dynamic processes with increasing the treatment temperature from 120 to 500 â: (1) the slow crystallization of PPTA (120~165 â), (2) the dissociation of adsorptive H2O2 and the desorption of adsorptive H2O (165~236 â), (3) the quick decomposition of tungsten oxide hydrate (236~287 â) and (4) the crystallization and phase transformation of the final products WO3 (287~500 â). Optical absorption of PPTA gels happens in the range of 350~600 nm, where the intensity of the optical absorption gradually increases and finally reaches the saturation with the increase of photon energy. The optical band gap was estimated to be about 2.25 eV, being obviously lower than the known values for WO3 and H2WO4 (2.48~3.50 eV). The key factors for the low gap value of the composite can be attributed to the molecular water, the oxygen defects and the structural distortion.
ABSTRACT
The power consumption and latency of existing MAC protocols for wireless sensor networks (WSNs) are high in heterogeneous convergecast, where each sensor node generates different amounts of data in one convergecast operation. To solve this problem, we present W-MAC, a workload-aware MAC protocol for heterogeneous convergecast in WSNs. A subtree-based iterative cascading scheduling mechanism and a workload-aware time slice allocation mechanism are proposed to minimize the power consumption of nodes, while offering a low data latency. In addition, an efficient schedule adjustment mechanism is provided for adapting to data traffic variation and network topology change. Analytical and simulation results show that the proposed protocol provides a significant energy saving and latency reduction in heterogeneous convergecast, and can effectively support data aggregation to further improve the performance.