ABSTRACT
The study explored the antimicrobial, antibiofilm, and hemostatic properties of chitosan microflowers (CMF) in sponge form. The main objective was to enhance the preparation of CMF by employing varying quantities of calcium chloride (CaCl2) and tripolyphosphate (TPP). CMF was then combined with gelatin (GE) in different proportions to produce three sponge samples: CMF0@GE, CMF1@GE, and CMF2@GE. The CMF had a morphology like that of a flower and produced surfaces with a porous sponge-like structure. The antibacterial activity, as determined by the zone of inhibition (ZOI), increased with greater doses of CMF. Among the tested samples, CMF2@GE had the greatest activity against Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, and Enterococcus faecium. CMF2@GE successfully suppressed biofilm formation, decreased clotting time to an average of 212.67 s, and exhibited excellent biocompatibility by preserving over 90 % viability of human skin fibroblast cells at dosages below 100 µg/mL. The results indicated that gelatin sponges filled with CMF have considerable promise as flexible medical instruments for wound healing and infection control.
ABSTRACT
BACKGROUND: It is necessary to develop advanced therapies utilizing natural ingredients with anti-inflammatory qualities in order to lessen the negative effects of chemotherapeutics. RESULTS: The bioactive N1-(5-methyl-5H-indolo[2,3-b]quinolin-11-yl)benzene-1,4-diamine hydrochloride (NIQBD) was synthesized. After that, soluble starch nanoparticles (StNPs) was used as a carrier for the synthesized NIQBD with different concentrations (50 mg, 100 mg, and 200 mg). The obtained StNPs loaded with different concentrations of NIQBD were coded as StNPs-1, StNPs-2, and StNPs-3. It was observed that, StNPs-1, StNPs-2, and StNPs-3 exhibited an average size of 246, 300, and 328 nm, respectively. Additionally, they also formed with homogeneity particles as depicted from polydispersity index values (PDI). The PDI values of StNPs-1, StNPs-2, and StNPs-3 are 0.298, 0.177, and 0.262, respectively. In vivo investigation of the potential properties of the different concentrations of StNPs loaded with NIQBD against MTX-induced inflammation in the lung and liver showed a statistically substantial increase in levels of reduced glutathione (GSH) accompanied by a significant decrease in levels of oxidants such as malondialdehyde (MDA), nitric oxide (NO), advanced oxidation protein product (AOPP), matrix metalloproteinase 9/Gelatinase B (MMP-9), and levels of inflammatory mediators including interleukin 1-beta (IL-1ß), nuclear factor kappa-B (NF-κB) in both lung and liver tissues, and a significant decrease in levels of plasma homocysteine (Hcy) compared to the MTX-induced inflammation group. The highly significant results were obtained by treatment with a concentration of 200 mg/mL. Histopathological examination supported these results, where treatment showed minimal inflammatory infiltration and congestion in lung tissue, a mildly congested central vein, and mild activation of Kupffer cells in liver tissues. CONCLUSION: Combining the treatment of MTX with natural antioxidant supplements may help reducing the associated oxidation and inflammation.
ABSTRACT
For drug delivery applications, silica nanoemulsion encapsulated with organic compounds are becoming increasingly more desirable. Therefore, the emphasis of this research was on the synthesis of a new potent antifungal drug-like candidate (1,1'-((sulfonylbis(4,1-phenylene)bis(5-methyl-1H-1,2,3-triazole-1,4-diyl))bis(3-(dimethylamino)prop-2-en-1-one), SBDMP), the chemical structure of which was confirmed on the basis of its spectral and microanalytical data. Then, silica nanoemulsion loaded with SBDMP was prepared using Pluronic F-68 as a potent surfactant. The particle shape, hydrodynamic size, and zeta potential of the produced silica nanoemulsion (with and without drug loading) were assessed. The antitumoral activity of the synthesized molecules showed the superiority of SBDMP and silica nanoemulsion with and without SBDMP loading against Rhizopus microsporous and Syncephalastrum racemosum. Subsequently, the laser-induced photodynamic inactivation (LIPDI) of Mucorales strains was determined using the tested samples. The optical properties of the samples were investigated using UV-vis optical absorption and the photoluminescence. The photosensitivity of the selected samples appeared to enhance the eradication of the tested pathogenic strains when exposed to a red (640 nm) laser light. The optical property results verified that the SBDMP-loaded silica nanoemulsion has a high depth of penetration into biological tissues due to a two-absorption photon (TAP) mechanism. Interestingly, the photosensitizing of the nanoemulsion loaded with a newly synthesized drug-like candidate, SBDMP, opens up a new route to apply new organic compounds as photosensitizers under laser-induced photodynamic therapy (LIPDT).
ABSTRACT
In the last decades, bio-based active food packaging materials have received much attention. It is known that the utilization of traditional materials for food packaging applications lack some critical characteristics such as resistance to the harmful microbes that cause a damage to the preserved foods. Therefore, the current study aimed to find an alternative packaging films comprises an efficient biopolymers. This research work was designed to prepare film mats using hydroxypropyl starch (HPS), polyvinyl alcohol (PVA), palmitic acid (PA) and biosynthesized zinc oxide nanoparticles (ZnONPs). The fabricated films were coded as 1H, 2H, 3H and 4H based on the utilized concentration of ZnONPs. The biosynthesized ZnONPs and the bio-based films loaded with ZnONPs were characterized. The results revealed that ZnONPs exhibited nearly spherical shape and size â¼40 nm. The surface structure of the produced bioactive packaging films exhibited smooth with homogeneous features, excellent mechanical and thermal stability properties. The prepared bioactive packaging film loaded with ZnONPs (4H) exhibited superior antibacterial activity among other films against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923 with inhibition zones 15.1 ± 0.76 and 12.1 ± 0.71 mm respectively. Correspondingly, packing film 4H exhibited potential antifungal activity toward Aspergillus niger RCMB 02724, A. flavus RCMB 02782, Penicillium expansum IMI 89372 and Fusarium oxysporum RCMB 001004 with inhibition zones (16 ± 1.0, 22 ± 0.90, 18.0 ± 1.1 and12.3 ± 0.57 mm respectively). Moreover, all prepared films did not show cytotoxicity on the normal cell line (Wi38) and recorded biodegradability properties that reached around 85 % after four weeks in soil. Based on these results, the antimicrobial films comprising HPS/PVA and loaded with the biosynthesized ZnONPs can be considered as a suitable film for food packaging purposes.
Subject(s)
Anti-Infective Agents , Nanoparticles , Zinc Oxide , Polyvinyl Alcohol/chemistry , Food Packaging/methods , Zinc Oxide/chemistry , Starch/chemistry , Anti-Infective Agents/pharmacology , Anti-Infective Agents/chemistry , Anti-Bacterial Agents/pharmacology , Anti-Bacterial Agents/chemistry , Nanoparticles/chemistryABSTRACT
As estrogen production decreases during menopause; the brain's metabolism tends to stall and become less effective. Estrogen most likely protects against neurodegeneration. Consequently, a comprehensive study of the benefits of hormone replacement therapy as a neuroprotective effect is urgently required. This study was designed to fabricate pumpkin seed oil nanoparticles (PSO) in nanoemulsion form (PSO-NE) and investigate their potential role in attenuating the neural-immune interactions in an experimental postmenopausal model.Sixty female white albino rats were divided into six groups: control, sham, ovariectomized (OVX), and three OVX groups treated with 17ß-estradiol, PSO, and PSO-NE respectively. Transmission Electron Microscopy (TEM), and particle size analyzer were performed for nanoemulsion evaluation. Serum levels of estrogen, brain amyloid precursor protein (APP), serum levels of nuclear factor kappa B (NF-κß), interleukin 6 (IL-6), transthyretin (TTR), and synaptophysin (SYP) were evaluated. The expression of estrogen receptors (ER-α, ß) in the brain tissue was estimated. The findings revealed that the approached PSO-NE system was able to reduce the interfacial tension, enhance the dispersion entropy, lower the system free energy to an extremely small value, and augment the interfacial area. PSO-NE, showed a significant increase in the levels of estrogen, brain APP, SYP, and TTR accompanied with a significant increased in the expression of brain ER-α, ß compared to the OVX group. In conclusion, the phytoestrogen content of PSO exhibited a significant prophylactic effect on neuro-inflammatory interactions, ameliorating both estrogen levels and the inflammatory cascades.
Subject(s)
Cucurbita , Estrogen Replacement Therapy , Female , Estradiol/pharmacology , Estrogens/pharmacology , Postmenopause , Animals , RatsABSTRACT
This study aims to fully exploit the natural compound; bee venom (BV) as a substance that can kill and inhibit the growth of microbes and viruses. For this target, BV was loaded onto a safe, natural, and economically inexpensive polymer; chitosan (Ch) in its nano-size form prepared using ionic gelation method in the presence of chemical crosslinking agent (sodium tripolyphosphate; TPP). The findings illustrated that chitosan nanoparticles (ChNPs) were prepared thru this method and exhibited spherical shape and average hydrodynamic size of 202 nm with a polydispersity index (PDI = 0.44). However, the size was increased to 221 nm with PDI (0.37) when chitosan nanoparticles were loaded with BV (ChNC). In addition, the particles of BV appeared as a core and chitosan nanoparticles as a shell implying the successful preparation of nanocomposite (ChNC). Encapsulation of BV into ChNPs with significantly small size distribution and good stability that protect these formed nanocomposites from agglomeration. The cytopathic effect (CPE) inhibition assay was used to identify potential antivirals for Middle East respiratory syndrome coronavirus (MERS-CoV). The response of the dose study was designed to influence the range of effectiveness for the chosen antiviral, i.e., the 50 % inhibitory concentration (IC50), as well as the range of cytotoxicity (CC50). However, our results indicated that crude BV had mild anti-MERS-COV with selective index (SI = 4.6), followed by ChNPs that exhibited moderate anti-MERS-COV with SI = 8.6. Meanwhile. The nanocomposite of ChNC displayed a promising anti-MERS-COV with SI = 12.1. Additionally, the synthesized nanocomposite (ChNC) had greater antimicrobial activity against both Gram-positive and Gram-negative bacteria when compared with ChNPs, BV or the utilized model drug.
Subject(s)
Bee Venoms , Chitosan , Middle East Respiratory Syndrome Coronavirus , Nanoparticles , Chitosan/chemistry , Anti-Bacterial Agents , Gram-Negative Bacteria , Gram-Positive Bacteria , Nanoparticles/chemistry , Drug Resistance, Multiple , Particle SizeABSTRACT
To examine the utilization of metal oxide nanoparticles (NPs) in different commercial products, this work focuses on the determination of cost-effective and scalable synthesis protocols. The solvothermal protocol is well-known as a scalable method but has recently been shown to lack economic feasibility. The mechanochemical method has recently been recognized to be a more economic and environmentally friendly substitute for the solvothermal method. In this study, zinc oxide nanoparticles (ZnO NPs) and copper oxide nanoparticles (CuO NPs) were synthesized using two (aqueous and organic) solvothermal (wet) methods and two (manual and automated) mechanochemical (dry) methods. The four methods were evaluated and compared. The automated mechanochemical method generated a significantly higher yield of ZnO NPs (82%) and CuO NPs (84%) using the least energy and time. However, the prepared ZnO NPs displayed higher cytotoxicity against Vero E6 cells when compared to that of CuO NPs. Because of their low cytotoxicity, CuO NPs synthesized via the automated mechanochemical method were selected for application onto cotton fabrics. Lower cytotoxicity was observed for CuO NPs treated fabrics with an IC50 of 562 mg/mL and ZnO treated fabrics with an IC50 at 23.93 mg/mL when the treated fabrics were tested against L929 fibroblast cells. Additionally, the cotton fabrics retained bactericidal and virucidal effects after four washes. Thus, the current study recommends the automated mechanochemical method as a cost-effective scalable approach for the synthesis of CuO NPs. The application of CuO NPs onto cotton fabrics generated washable antimicrobial face masks.
ABSTRACT
BACKGROUND: Liver inflammation is a multistep process that is linked with cell membrane fatty acids composition. The effectiveness of eicosapentaenoic acid (EPA) undergoes an irreversible change during processing due to their unsaturated nature; so the formation of nanocarrier for EPA is crucial for improving EPA's bioavailability and pharmacological properties. OBJECTIVE: In this study we aimed to evaluate the efficiency of EPA alone or loaded silica nanoemulsion on the management of hepatic inflammation induced by diethyl nitrosamine (DEN) through the enhancement of the cell membrane structure and functions. METHODS: The new formula of EPA was prepared to modify the properties of EPA. Forty-eight male Wistar albino rats were classified into: control, EPA, EPA loaded silica nanoemulsion (EPA-NE), DEN induced hepatic inflammation; DEN induced hepatic inflammation treated with EPA or EPA -NE groups. Plasma tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1ß), liver hydroxyproline (Hyp) content, and liver oxidant and anti-oxidants were estimated. Urinary 8- hydroxyguanozine (8- OHdG) and erythrocyte membrane fatty acids fractions were estimated by High-performance liquid chromatography (HPLC). Also, histopathology studies were done to verify our hypothesis. RESULTS: It was appeared that administration of EPA, in particular EPA loaded silica nanoemulsion, ameliorated the inflammatory response, increased the activity of the anti-oxidants, reduced levels of oxidants, and improved cell membrane structure compared to hepatic inflammation induced by DEN group. Histopathological examination confirmed these results. CONCLUSION: EPA and notably EPA loaded silica nanoemulsion strongly recommended as a promising supplement in the management of hepatic inflammation.
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Bionanocomposites (BNC1,2) of binary (PPCH-Ag) and ternary (PPCH-TiO2-Ag) (PPCH = polyphosphonium chitosan-hydrazone) have been synthesized and immobilized on cellulosic fabrics (CFs) using an environmentally friendly single-step in situ methodology. The results of FTIR, TGA, EDX, SEM, and TEM investigations showed that PPCH and its BNCs were successfully formed on the surface layer of fabrics. Moreover, the BNC2-coated cloth exhibited a superhydrophobic behavior as revealed from the values of water contact angle (WCA) 152.1° and slide angle (SA) 8.7°. The cytotoxicity experiments on epithelial cells confirmed the safety of treated fabrics for human cells. The antimicrobial capabilities of the BNCs-treated textiles were greatly enhanced, with a small preference for BNC1-coated fabric, as compared to the native or other treated fabrics. In contrast, the BNC2-coated fabric demonstrated the highest anti-UV protection capabilities as indicated by its great capacity to reduce the UV transmission (UV-A, 2.1 %; UV-B, 1.8 %) as well as its UPF value (49.2). The durability tests revealed the high resistance of BNC2-CF against harsh washing conditions and their acquired functions sustainability up to 20 washing cycles.
Subject(s)
Chitosan , Chitosan/chemistry , Humans , Hydrazones , Textiles , Titanium , Ultraviolet Rays , WaterABSTRACT
This study reports the preparation of a new material that can remove synthetic dyes and trace metals simultaneously. A new coumarin derivative was synthesized and its chemical structure was inferred from spectral data (FT-IR, 1H-NMR, 13C-NMR). Meanwhile, chitosan nanoparticles (CsNPs) were prepared then used as a carrier for two different concentrations of the coumarin derivative (C1@CsNPs and C2@CsNPs). The TEM, SEM and DLS findings illustrated that the prepared nanocomposites exhibited spherical shape and small size (less than 200 nm). The performance of the prepared material for the removal of an anionic dye (direct red 31, DR31) and cationic trace metal (Pb2+) was evaluated in unary and binary systems. The results revealed that complete removal of 10 mg L-1 of DR31 and Pb2+ in unary system was achieved at pHo 3.0 and 5.5 using 0.5 and 2.0 g L-1, respectively, of C2@CsNPs. The adsorption of DR31 and Pb2+ followed different mechanisms as deduced from the effect of pHo, kinetic, isotherm and binary adsorption studies. The adsorption of DR31 followed the Langmuir isotherm model and the pseudo-first-order kinetic model. While, the adsorption of Pb2+ followed Freundlich isotherm model and Elovich kinetic model. In the binary system, the co-presence of DR31 and Pb2+ did not affect the adsorption of each other's. Overall, the prepared material showed promising results for the removal of anionic dyes and cations trace metals from contaminated water.
ABSTRACT
Plastic products are widely used in different applications. Thus, exposure of human and other organisms to these products may affect their biological system. The current study was conducted to investigate the potential deleterious effect of Polysterene nanoparticles (PS-NPs) on the liver and to state the cellular and molecular mechanisms associated with exposure to PS-NPs.30 male rats were divided randomly and equally into 3 groups; control (distilled water), low dose (3 mg/kg/day) and high dose (10 mg/kg/day) exposed group via oral gavage for 5 successive weeks. PS-NPs caused elevation in ALT, AST and MDA, upregulation of apoptosis-related genes and significant decrease in GSH and mRNA expression for antioxidant-related genes (Nrf-2 and GPx). Moreover, alterations in hepatic tissue architecture and positive caspase-3 expression was noticed in a dose- dependent manner. Collectively, PS-NPs can induce hepatoxicity in rats in a dose dependent manner, so the health risk of PS-NPs should not be ignored.
Subject(s)
Liver Diseases , Nanoparticles , Animals , Apoptosis , Hepatocytes/metabolism , Humans , Liver , Liver Diseases/metabolism , Male , Nanoparticles/toxicity , Oxidative Stress , Polystyrenes/toxicity , RatsABSTRACT
Water decontamination from toxic dyes and pathogenic microorganisms is critical for life on Earth. Herein, we report the synthesis of sulfone biscompound containing 1,2,3-triazole moiety and evaluation of its dye decolorization and biocidal and disinfection efficiencies. The decolorization efficiency was tested under different experimental conditions, while the biocidal action was examined against various types of waterborne pathogens, and the disinfection of some pathogenic microbes was executed in artificially contaminated water. The findindgs illustrated that the solution initial pH (pHi) affected the decolorization efficiency significantly. About complete removal of 10 mg/L malachite green (MG) dye was achieved after 10 min using 3 g/L of the sulfone biscompound at pHi 6. The pseudo-second-order equation suited the adsorption kinetics accurately, while the equilibrium data was suited by Langmuir isotherm model. Electrostatic, n-π, and π-π interactions brought about the adsorption of MG onto the sulfone biscompound. The biocidal results indicated that the sulfone biscompound had a powerful antibacterial potential against the tested bacterial species. Likewise, the distinction trail revealed that after 70-90 min of direct contact with an effective dose, the tested pathogens could be completely eliminated (6-log reduction). Overall, the newly synthesized sulfone biscompound can efficiently remove cationic dyes and disinfect contaminated water.
Subject(s)
Disinfection , Water Pollutants, Chemical , Triazoles/pharmacology , Coloring Agents/chemistry , Adsorption , Kinetics , Water Pollutants, Chemical/chemistry , Water/chemistry , Sulfones , Anti-Bacterial Agents , Hydrogen-Ion Concentration , ThermodynamicsABSTRACT
The target of our current work was designed to prepare titanium oxide doped silver nanoparticles (Ag/TiO2 NPs) and their impact on the functionalization of cotton fabrics. Additionally, the effect of Ag/TiO2 NPs was compared with the individually prepared silver nanoparticles (AgNPs) and titanium oxide nanoparticles (TiO2 NPs). In this work, AgNPs were prepared in the solid state using arabic gum as efficient stabilizing and reducing agent. Then, two concentrations of the as-synthesized nanoparticles were used to functionalize the cotton fabrics by pad-dry-cure treatment in the presence of fixing agent to increase the durability of treated cotton fabrics against vigorous washing cycles. The findings implied that the as-prepared nanoparticles were successfully synthesized in nano-size with spherical shape and homogeneity. The efficacy of the functionalized cotton fabrics with those nanoparticles were evaluated in terms of multifunctional properties including antimicrobial and ultraviolet protection factor (UPF) and the mechanical features before and after many washing cycles; 10, 15 and 20 times. The resultant also proved that Ag/TiO2 NPs-treated cotton fabrics exhibited the greater values of both antimicrobial and UPF properties with enhancement in the tensile strength and elongation features. Thus, the combination between these two nanoparticles through doping reaction is suitable for imparting superior antimicrobial properties against the four tested microbial species (Staphylococcus aureus, Escherichia coli, Candida albicans, and Aspergillus niger) and good UPF properties. Depending on the promising obtained results of the multi-finishing fabrics, these nanoparticles of Ag/TiO2 NPs can be applied for the production of an efficient medical clothes for doctors, nurses and bed sheets for patients in order to kill and prevent the spread of bacteria and then, reduce the transmission of infection to others.
Subject(s)
Anti-Infective Agents , Metal Nanoparticles , Anti-Bacterial Agents/pharmacology , Anti-Infective Agents/pharmacology , Cotton Fiber , Escherichia coli , Humans , Silver/pharmacology , TitaniumABSTRACT
Current research targets innovative medical textiles of nanofibrous nature and antibacterial activity to prevent diaper dermatitis. The work is based on electrospun nanofibers from cellulose acetate (CA) and lignin (Lig) polymers. A series of new copper complexes were synthesized and loaded to the CA/Lig solution mix then subjected to electrospinning, giving rise to the tricomponent bioactive mats CA/Lig/Cu-complex. The surface morphology of electrospun nanofiber mats was smooth and homogenous as the concentration of lignin increased in the mixture. The incorporation of lignin improved the electrospinnability of the cellulose acetate; however, it increased the fiber diameter. The water contact angle, absorption underload were significantly improved as lignin content increased. The incorporation of Cu-complex in electrospun CA and CA/Lig fiber mats occurred without any substantial change in the surface morphology, indicating well encapsulation of the complex. The electrospun mats were active against Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus epidermidis, and Streptococcus faecalis. The cytotoxicity, protein leakage, and biological results, together with the above studies, would advocate copper complex loaded CA/Lig nanofibers as a potential candidate for hygienic applications.
Subject(s)
Dermatitis , Nanofibers , Cellulose/analogs & derivatives , Copper , Humans , Lignin/pharmacologyABSTRACT
The naturally occurring neocryptolepine (5-Methylindolo [2,3-b]quinoline) and its analogs exhibited prominent anticancer and antimalarial activity. However, the main problem of this class of compounds is their poor aqueous solubility, hampering their bioavailability and preventing their clinical development. To overcome the problem of insolubility and to improve the physicochemical and the pharmacological properties of 5-Methylindolo [2,3-b]quinoline compounds, this work was designed to encapsulate such efficient medical compounds into mesoporous silica oxide nanoemulsion (SiO2NPs). Thus, in this study, SiO2NPs was loaded with three different concentrations (0.2 g, 0.3, and 0.6 g) of 7b (denoted as NPA). The findings illustrated that the nanoparticles were formed with a spherical shape and exhibited small size (less than 500 nm) using a high concentration of the synthesized chemical compound (NPA, 0.6 g) and good stabilization against agglomeration (more than -30 mv). In addition, NPA-loaded SiO2NPs had no phase separation as observed by our naked eyes even after 30 days. The findings also revealed that the fabricated SiO2NPs could sustain the release of NPA at two different pH levels, 4.5 and 7.4. Additionally, the cell viability of the produced nanoemulsion system loaded with different concentrations of NPA was greater than SiO2NPs without loading, affirming that NPA had a positive impact on increasing the safety and cell viability of the whole nanoemulsion. Based on these obtained promising data, it can be considered that the prepared NPA-loaded SiO2NPs seem to have the potential for use as an effective anticancer drug nanosystem.
Subject(s)
Alkaloids/pharmacology , Antineoplastic Agents/pharmacology , Delayed-Action Preparations/chemistry , Nanoparticles/chemistry , Quinolines/pharmacology , Alkaloids/administration & dosage , Alkaloids/chemical synthesis , Alkaloids/chemistry , Antineoplastic Agents/administration & dosage , Antineoplastic Agents/chemical synthesis , Antineoplastic Agents/chemistry , Cell Line, Tumor , Cell Survival/drug effects , Drug Liberation , Humans , Hydrogen-Ion Concentration , Neoplasms/drug therapy , Quinolines/administration & dosage , Quinolines/chemical synthesis , Quinolines/chemistry , Silicon Dioxide/chemistryABSTRACT
Environmentally-friendly, cyanidin(Cy)-based anthocyanin isolated from red-cabbage served as a spectroscopic probe imprinted onto chitosan nanoparticles (CsNPs), which were in turn integrated onto cellulose paper strip (CPS) as a host matrix to develop a metallochromic solid state sensor for real-time selective determination of ferric ions in an aqueous medium. The ferric transition metal ions in aqueous environments were detected using a novel, simple, portable, fast responsive, low-cost, real-time, environmentally safe, reversible and colorimetric sensor based on chitosan nanoparticles as a hosting biopolymer and cyanidin phenol chromophore as a biomolecular probe. In order to use the cyanidin biomolecule as a pH indicator and chelating agent, it was purified from red-cabbage and added into the CsNPs biosensor film. The colorimetric shift increased in direct proportion to the ferric ion concentration. As a result, the current research that was both qualitative and quantitative was carried out. While the Cy-CsNPs-CPS sensor showed high selectivity for ferric ions, no color change was detected for other metal cations. It was discovered that the detection process occurred as a result of a coordination complex formed between the active sites of phenolic cyanidin and Fe(III) ions.
Subject(s)
Brassica , Chitosan , Nanoparticles , Anthocyanins/chemistry , Brassica/chemistry , Cellulose/chemistry , Ferric Compounds , Plant ExtractsABSTRACT
In this work, a comparative study was made of different magnesium ion content incorporated into hydroxyapatite (HAP) and modified with selenite ions, with the aim to develop the degradation performance of methylene blue. Although the dopant metal (Mg2+ ) was present at a relatively low ratio, it induced a change in the microstructure, morphology, surface area, external surface charge, particle size, and degradation performance. The effect of magnesium and selenium binary doping on microstructure and degradation of methylene blue was evaluated. The external surface charge measured by zeta potential clarified that the highest negativity was -11.8 mV and this was accomplished in 1.0 Mg/Se-HAP. Furthermore, the roughness average increased from 36.8 nm, reaching 59.2 nm upon the addition of Mg(II). Moreover, transmission electron microscope micrographs showed that compositions were formed as rod shapes. The process of degradation was optimized, showing effectiveness in methylene blue degradation of 62.4% after 150 min of exposure to visible light. Electrostatic attraction and H-bonding, and coordination played vital roles in the adsorption process. The recyclability of the as-prepared compositions demonstrated that the effectiveness had been reduced to ~54.2% after five times of re-use.
Subject(s)
Methylene Blue , Water Pollutants, Chemical , Adsorption , Durapatite , Ions , Magnesium , Methylene Blue/chemistry , Water Pollutants, Chemical/analysisABSTRACT
Polystyrene Nanoparticles (PS-NPs) used for packaging foam, disposable cups, and food containers. Therefore, this study aimed to evaluate PS- NPs toxic effects on kidney of adult male albino rats. A total of 30 rats divided into three groups (n = 10): group I negative control group; group II orally administered 3% PS-NPs (3 mg/kg body weight/day) and group III orally administered 3% PS-NPs (10 mg/kg body weight/day) for 35 days. Blood and kidney samples collected and processed for biochemical, histopathological, and immunohistochemical examinations. Results showed that low and high doses PS-NPs had significantly increased serum blood urea nitrogen (BUN), creatinine, malondialdehyde, significantly further reduced glutathione, downregulation of nuclear factor erythroid 2-related factor 2 and glutathione peroxidase, upregulation of caspase-3 and Cytochrome-c. Histopathological examination revealed several alterations. Low dose of PS-NPs exhibited dilated glomerular capillaries, hypotrophy of some renal corpuscles significantly decreases their diameter to 62 µm. Some proximal convoluted tubules and distal convoluted tubules showed loss of cellular architecture with pyknotic nuclei. Hyalinization and vacuolation in renal medulla. In high dose PS-NPs, alterations increased in severity. A significant increase in percentage area of cyclooxygenase-2 in low and high-doses. In conclusion, PS-NPs are a nephrotoxic causing renal dysfunction.
Subject(s)
Kidney Diseases , Nanoparticles , Animals , Kidney/metabolism , Kidney Diseases/metabolism , Male , Malondialdehyde/metabolism , Nanoparticles/toxicity , Oxidative Stress , Polystyrenes/analysis , Polystyrenes/metabolism , Polystyrenes/toxicity , RatsABSTRACT
There have been strong demands for nanofibrous scaffolds fabricated by electrospinning for various fields due to their various advantages. Electrospun poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) fibre mats were prepared. The effects of processing variables as well as the inclusion of poly(ethylene glycol) (PEG) on the morphologies of generated fibres were investigated using Fourier-transform infrared spectroscopy and scanning electron microscopy. The average fibrous diameter was monitored in the range 400-3000 nm relying on the total content of PEG. The fluorescence cell imaging of electrospun mats was also explored. The results of cell viability demonstrated that skin fibroblast BJ-1 cells showed different adhesions and growth rates for the three kinds of PHBV fibres. Electrospun PHBV mats with low amount of PEG offer a high-quality medium for cell growth. Therefore, those mats exhibited high potential for soft tissue engineering, in particular wound healing.
Subject(s)
Nanofibers , Tissue Engineering , 3-Hydroxybutyric Acid , Cell Proliferation , Hydroxybutyrates , Polyesters , Polyethylene GlycolsABSTRACT
Designing a nanocomposite that accumulates biocompatibility and antimicrobial behaviour is an essential requirement for biomedical applications. Hydroxyapatite (HAP), graphene oxide, and vivianite in one ternary nanocomposite with three phases and shapes led to an increase in cell viability to 97.6% ± 4 for the osteoblast cells in vitro. The obtained nanocomposites were investigated for their structural features using X-ray diffraction, while the microstructure features were analyzed using a scanning electron microscope (SEM) and a transmission electron microscope. The analysis showed a decrease in the crystal size to 13 nm, while the HAP grains reached 30 nm. The elongated shape of vivianite reached 200 nm on SEM micrographs. The monoclinic and hexagonal crystal systems of HAP and vivianite were presented in the ternary nanocomposite. The maximum roughness peak height reached 236.1 nm for the ternary nanocomposite from 203.3 nm, while the maximum height of the roughness parameter reached 440.7 nm for the di-nanocomposite of HAP/graphene oxide from 419.7 nm. The corrosion current density reached 0.004 µA/cm2 . The ferrous (Fe2+ ) and calcium (Ca2+ ) ions released were measured and confirmed. Therefore, the morphology of the nanocomposites affected bacterial activity. This was estimated as an inhibition zone and reached 14.5 ± 0.9 and 13.4 ± 1.1 mm for Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) after 24 h. The increase in viability and the antibacterial activity refer to the compatibility of the nanocomposite in different medical applications.