ABSTRACT
Achieving full and stable employment is not only one of the goals of macro-control by governments but also a key issue that labor migrants must address. To understand the impact of relocation adaptation on the employment stability of Chinese-style labor migrants, members of the group visited the labor migrant settlement sites in Yinchuan City and used questionnaires to investigate the adaptation and employment status of farmers after relocation. The article attempts to analyze the impact of relocation adaptability, embodied by social adaptability, economic adaptability, and cultural adaptability, on the employment stability of Chinese-style labor migrants using structural equation modeling with the highly representative field research data from the labor migrant community in Yinchuan City as an example. The results of the study show that the social, economic, and cultural adaptability dimensions of relocation adaptability all have a significant positive effect on employment stability. Therefore, to promote the stable employment of Chinese-style labor migrants, it is necessary to enhance economic adaptability to stabilize employment and increase income, enhance social adaptability to proactively adapt and actively participate, and strengthen cultural adaptability to proactively seek change and actively adapt to better improve the employment situation of labor migrants in an orderly manner.
Subject(s)
Emigration and Immigration , Employment , Transients and Migrants , China , Humans , Transients and Migrants/psychology , Female , Male , Adult , Surveys and Questionnaires , Job Security , East Asian PeopleABSTRACT
The cotton bollworm, Helicoverpa armigera, is one of the most destructive pests in the world. A combination of molecular genetics, physiology, functional genomics, and behavioral studies has made H. armigera a model species in Lepidoptera Noctuidae. To study the in vivo functions of and interactions between different genes, clustered regularly interspaced short palindromic repeats (CRISPR)/ associated protein 9 (Cas9) genome editing technology is a convenient and effective method used for performing functional genomic studies. In this study, we provide a step-by-step systematic method to complete gene knockout in H. armigera using the CRISPR/Cas9 system. The design and synthesis of guide RNA (gRNA) are described in detail. Then, the subsequent steps consisting of gene-specific primer design for guide RNA (gRNA) creation, embryo collection, microinjection, insect rearing, and mutant detection are summarized. Finally, troubleshooting advice and notes are provided to improve the efficiency of gene editing. Our method will serve as a reference for the application of CRISPR/Cas9 genome editing in H. armigera as well as other Lepidopteran moths.
