Exogenous N-acyl-homoserine lactones accelerate resuscitation of starved anaerobic granular sludge after long-term stagnation.

So as to accelerate the resuscitation of starved anaerobic granular sludge after long-term stagnation, an innovative method was tried derived from the regulation of N-acyl-homoserine lactones (AHLs)-mediated quorum sensing (QS). The mixture of four AHLs was added to the starved anaerobic granular sludge system in this research. The results confirmed that the exogenous AHLs shortened the recovery time of the granular sludge, and improved the treatment performance and methanogenic capacity of the recovered anaerobic sludge to the level before stagnation. At the same time, exogenous AHLs enhanced the synthesis of extracellular polymeric substances (EPS) during the resuscitation period of starved anaerobic granular sludge. The outcomes of microbial composition detection showed that the change of bacterial and methanogenic bacteria communities towards accelerated performance recovery was significantly correlated with exogenous AHLs. This exploration provided a new technical idea for speeding up the recovery of starved anaerobic granular sludge.

Exogenous N-acyl-homoserine lactones promote the degradation of refractory organics in oligotrophic anaerobic granular sludge.

For refractory industrial wastewaters, anaerobic granular sludge technology cannot be widely used because of its limited treatment capacity, so strengthening the anaerobic degradation of refractory organics should be discussed. In this paper, the feasibility of adding exogenous N-acyl-homoserine lactones (AHLs) to promote the degradation of refractory organics in oligotrophic anaerobic granular sludge was addressed. The results showed that, after easily-degradable organics were completely metabolized, exogenous AHLs strengthened the further degradation of refractory organics and improved the methanogenic activity of anaerobic granular sludge. In addition, adding AHLs could promote the secretion of more extracellular polysaccharides and proteins by anaerobic microorganisms to resist the oligotrophic environment. Microbiological analysis showed that adding AHLs significantly optimized the microbial community in oligotrophic anaerobic granular sludge. With the regulation of AHLs, the abundance proportion of hydrolytic acidifying bacteria for refractory organics in bacterial community and the abundance proportion of acetotrophic methanogens in methanogens community increased obviously. Exogenous AHLs showed concentration-related effects on the optimization of bacteria and methanogens, and AHLs of higher concentration were beneficial to the succession of community structure in a better direction. Exogenous regulation of AHLs-mediated QS provided an attractive strategy for enhancing the anaerobic degradation of refractory organics, and proposed a technical idea for the application of anaerobic granular sludge technology in refractory industrial wastewaters.