ABSTRACT
The use of nanotechnology products with supermagnetic properties for targeted delivery of drugs has gained attention recently. Due to the anticancer features of Gingerol, the major phenolic compound from Ginger, this study aims to prepare Fe3O4@Glucose-Gingerol nanoparticles (NPs) and investigate their anticancer potential in a lung adenocarcinoma cell line. The physical and chemical features of the nanoparticles were investigated by FT-IR, XRD, zeta potential, DLS, EDS mapping, VSM, and electron microscope imaging. Cytotoxic effects of the nanoparticles for the A549 (lung adenocarcinoma) and MRC-5 (normal) cell lines was investigated by MTT assay. Furthermore, the effects of Fe3O4@Glucose-Gingerol nanoparticles on the expression of the CASP8, BAX, and BCL2 genes and the activity of Caspase 3 were characterized. The flow cytometry assay (annexin V/PI) was employed to find out the percentage of apoptotic cells. The Fe3O4@Glu-Gingerol NPs were spherical (42-67 nm), without elemental impurity, and with surface charge, DLS size, and magnetic saturation of -47.7 mV, 154 nm, and 35 emu/g, respectively. Fe3O4@Glu-Gingerol NPs showed a remarkable greater toxicity in the A549 cells than normal cell line with the 50 % inhibition concentration (IC50) of 190 and 554 µg/mL, respectively. Treatment of lung adenocarcinoma cells with the Fe3O4@Glu-Gingerol NPs led to an increase in cell apoptosis from 4.6 to 39.48 %. Also, the CASP8 and BAX genes were upregulated by 2.49 and 2.8 folds, respectively, while a downregulation by 0.75 folds was noticed for the BCL2. Moreover, apoptotic features were observed in Fe3O4@Glu-Gingerol NPs treated cells by Hoechst staining, and activation of Caspase 3 by 2.8 folds. This study revealed that the Fe3O4@Glu-Gingerol NPs have antiproliferative effects on the lung adenocarcinoma cell line by activation of intrinsic and extrinsic apoptosis that is a promising feature in cancer treatment.
ABSTRACT
Background: Stabilizing exercises reduce pain intensity, improves shoulder position and scapular function, and provides an appropriate strategy for the improvement of scapular dyskinesia. The purpose of this study was to investigate the effect of six weeks of stability exercises (stretching-strengthening) on joint proprioception, strength, and range of motion of the glenohumeral joint in female tennis players with scapular dyskinesia. Methods: Thirty-six female elite tennis players with scapular dyskinesia in both experimental and control groups participated in this study. Goniometer, Isokinetic and Biodex devices were used to evaluate the range of motion, internal and external rotation strength in 60° and 180°, and joint proprioception at 45° and 60°, respectively. Also, the lateral scapular slide test (LSST) was used to evaluate the scapulohumeral rhythm. For analyzing dependent variables and determining statistical significance the ANCOVA and an alpha of 5% was used. Results: The results of this study indicated the effect of the stability exercise program on the range of motion of internal (p = 0.016) and external (p = 0.023) rotation of the shoulder. Also, significant differences were observed between the control and training groups for internal rotation strength 60° (p = 0.013), 180° (p = 0.017) and external rotation strength 60° (p = 0.005), 180° (p = 0.045) and strength ratio 60° (p = 0.001) and 180° (p = 0.023). However, there were no significant differences for proprioception. Conclusion: In general, the findings of this study support the effectiveness of exercise therapy as a safe intervention for improving scapular function in tennis players with scapular dyskinesia.
ABSTRACT
Background: Several interventions have been used to relieve chronic low back pain (CLBP). This study aimed to compare the effects of pain neuroscience education (PNE) followed by motor control exercises (MCEs) with core stability training (CST) on pain, disability, and balance in women with CLBP. Methods: Thirty-seven women with CLBP were randomly divided into two groups of PNE/MCE (n = 18, 55.2 ± 2.6 years) or CST (n = 19, 54.6 ± 2.4 years). Eight weeks of PNE/MCE or CST were prescribed for each group, independently. Pain intensity (VAS scale), disability (Roland Morris Disability Questionnaire), unipodal static balance, and dynamic balance (time up and go test) were measured at the beginning and 8 weeks after the intervention. Two-way mixed ANOVA was used to analyze the results with alpha of 5%. Results: After 8 weeks, there was a significant difference in VAS scale between groups (p = 0.024), with both PNE/MCE and CST showing 58% and 42% reductions, respectively. There were no differences for all other variables between groups. Regarding pre- to post-comparisons, both groups showed improvements in all dependent variables (p < 0.001). Conclusion: The treatment with PNE/MCE was more effective in improving pain disability and unipodal static and dynamic balance than treatment with CST. Even so, both treatments were shown to be valid and safe in improving all dependent variables analyzed in women with CLBP.