Temporal variations of LH and testosterone in arabian oryx (Oryx leucoryx) from birth to adulthood.

To determine the onset of puberty in Arabian oryx (Oryx leucoryx), a longitudinal study was conducted in 5 captive males at the National Wildlife Research Centre, Taif (Saudi Arabia). From birth to 20 months of age, twice weekly, animals were weighed and blood was assayed for LH and testosterone. Four periods were defined by analysis of the hormonal profile. During the first 5 months (corresponding to prepuberty), mean LH concentrations increased significantly while testosterone remained basal. Testosterone increased significantly during the second period (from 6 to 7 months) while LH decreased significantly. The testosterone pattern suggests that the animals reached puberty at around 7 months old. Mean LH concentrations remained at a similar level before and after puberty and increased significantly between the 12th and 13th months of life. This increment correspond to the transition between two further periods. The third period (8-12 months) was characterized by testosterone peaks of 2.4 +/- 0.1 ng ml-1 which irregularly occurred over a period of 26.6 +/- 2.7 days and was followed by a fourth period, during which peaks were seen at 24.1 +/- 1.6-day intervals and reached 4.7 +/- 0.2 ng ml-1. This last period probably corresponds to the mature stage of puberty. The testosterone cyclicity in oryx, coinciding with the estrous cycle length of the female, would permit the male to be fertile throughout the year and could be an adaptive response to the opportunistic and aseasonal reproductive cycle of the female.

Length of estrous cycle and gestation in the Arabian oryx (Oryx leucoryx) and the importance of the male presence for induction of postpartum estrus.

The reproduction of the Arabian oryx (Oryx leucoryx) has been studied in a breeding center in Taif (Saudi Arabia). In the first experiment, three groups of females were assigned to groups immediately after calving. In group 1, from the day of calving the females had visual and olfactory but not physical contact with a male. The calves were removed on the day of birth and each female was placed with a male on Day 45. In group 2, females and their calves were placed individually with a male the day after calving. In group 3, females and their calves were kept with neither visual nor olfactory contact with a male and then placed with a male on Day 90. The estrous cycle was deduced from assays of plasma progesterone up to Day 120 after calving. All females exhibited a postpartum estrus a few days after parturition but the occurrence of the estrus was significantly delayed in females of group 3. The length of the estrous cycle was 23.7 +/- 1.3 days and the gestation length was 255.2 +/- 1.5 days. In the second experiment, a group of adult females was formed in 1987 and the intervals between calving recorded over a period of 5 years. The mean interval between calving was 285 +/- 2 days (n = 99 gestations). However, 66.6% of these intervals averaged 276 days whereas 19.2% had averaged 294 and 13% averaged 321 days.

Concurrent avian pox and Newcastle disease infection in a Houbara bustard (Chlamydotts undulatd).

A case of acute respiratory disease was observed on a 3-month-old Houbara bustard (Chlamydotis undulatd) at the National Wildlife Research Center (NWRC) breeding unit. Newcastle disease (ND) and avian pox viruses were isolated from the lungs, spleen and trachea of the affected bird. A heavy secondary bacterial infection was also noticed. The clinical and pathological findings observed in this case are described. It is the first description of the isolation of ND virus from a Houbara bustard. The epidemiology of the disease in the breeding flock is discussed.

Genetic study of Gazella gazella: chromosomal and allozymic data.

Some subspecies of Gazella gazella are studied by means of protein electrophoresis (gazella, cora, and erlangeri) and cytogenetics (gazella, cora, erlangeri and farasani), and are compared to G. subgutturosa. Variability parameters and genetic distances are computed from allozyme data at 24 loci. Percentages of polymorphic loci range between 0 and 20.8%, mean numbers of alleles per locus between 1 and 1.25 and mean heterozygosities between 0 and 0.088. Inside G. gazella genetic distances range between 0.011 and 0.074 (Rogers' distance) and between 0.017 and 0.075 (Nei's distance). Genetic distances between subspecies of G. gazella and G. subgutturosa are around 0.16. C, GTG, and RBG banded karyotypes are given. The results are congruent with the subspecific status of G. g. gazella, G. g. cora, G. g. erlangeri and G. g. farasani, showing no karyological and only slight allozymic differences between them. These results may be important from a conservation point of view, for these populations whose ranges have been significantly declining over the last decades.

Serum chemistry values for Arabian sand gazelles (Gazella subgutturosa marica).

Values for urea, creatinine, glucose, total bilirubin, sodium, potassium, chloride, calcium, phosphates, magnesium, alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase, lactate deshydrogenase, and creatine-kinase are reported for the first time for 32 sand gazelles (Gazella subgutturosa marica) in Saudi Arabia. Comparisons were made between two groups: one sampled before a trip and the other sampled after a 14 hr trip. Only aspartate aminotransferase was higher in the second group; magnesium and phosphates were lower in that group.

An outbreak of tuberculosis in a captive herd of Arabian oryx (Oryx leucoryx): management.

An outbreak of tuberculosis due to Mycobacterium bovis in a herd of Arabian oryx (Oryx leucoryx) was managed by sanitary and medical measures. The sanitary measures included the isolation of infected animals, the monitoring of animals with a range of diagnostic tests and the hand-rearing of the progeny of the infected herd. All the infected oryx were treated with a mixture of isoniazid at 10 mg/kg bodyweight, ethambutol hydrochloride at 15 mg/kg bodyweight and rifampicin at 10 mg/kg bodyweight administered daily in the drinking water. These measures resulted in the cessation of mortalities, a decrease in the numbers of moderate and high risk animals and the production of tuberculosis-free animals for reintroduction into the wild.

An outbreak of tuberculosis in a captive herd of Arabian oryx (Oryx leucoryx): diagnosis and monitoring.

An outbreak of tuberculosis induced a mortality of 25 per cent in a captive herd of Arabian oryx (Oryx leucoryx). The diagnostic screening tests used on live animals included the comparative skin test, indirect and comparative ELISA tests and lymphocyte transformation tests. Difficulties in the interpretation of these tests stemmed principally from the facts that false negatives and false positives were encountered and that the threshold of positivity was difficult to establish with the ELISA test. The presence of other mycobacterial infections in the environment was almost certainly a complicating factor.

Chromosomal polymorphism in sand gazelles (Gazella subgutturosa marica).

A total of 84 Gazella subgutturosa from three captive populations (two in Saudi Arabia, one in Qatar) were karyotyped. The number of chromosomes is 33, 32, or 31 for the males and 32, 31, or 30 for the females because of the X-autosome translocation that is common in the genus and a centric fusion between the two pairs of acrocentric chromosomes. The G- and R-banded karyotypes of gazelles translocated show that this fusion is the same as that previously reported for gazelles from Jordan. The precise origin of these populations is not known, but in every case the first animals are said to come from the wild in Saudi Arabia. This chromosomal translocation appears to be a populational polymorphism and not the result of hybridization between two different subspecies of G. subgutturosa.

Chlamydiosis in a captive group of Houbara bustards (Chlamydotis undulata).

A chlamydiosis outbreak occurred in a Houbara bustard (Chlamydotis undulata) captive breeding group in Saudi Arabia, inducing peracute deaths, highly variable clinical signs, and pathological and histological lesions. Typical inclusion bodies in stained impression smears of spleen and prevalence (80%) of antibodies against Chlamydia, detected by a competitive enzyme immunoassay test, provided the bases for the diagnosis. This is the first report on a chlamydiosis outbreak in birds of the family Otididae.

An unusual Gazella dama karyotype.

The chromosomes of a female gazelle (Gazella dama) were investigated using GTG, RBG and C banding techniques. Their banding patterns showed that autosomes are involved in a complex of four Robertsonian translocations.

Pharmacokinetics of doxycycline after parenteral administration in the Houbara bustard (Chlamydotis undulata).

Fourteen adult Houbara bustards (Chlamydotis undulata) infected with Chlamydia psittaci were administered doxycycline at 100 mg/kg body weight in seven intramuscular or subcutaneous injections at intervals of 7, 7, 7, 6, 6, and 5 days. Blood levels of doxycycline were measured after the first and seventh injections at the following intervals: 0, 12, 24, 36, 48, 72, 96, 120, 144, 168, and 180 hours. During these two periods, most of the birds showed plasma doxycycline levels in excess of 1 micrograms/ml, demonstrating effective doxycycline levels for 45 days. A multifactorial analysis of variance revealed no significant differences between males and females, between the two routes of administration, or among all four factors.

Biochemical parameters following capture myopathy in one Arabian oryx (Oryx leucoryx).

Twenty-three biochemistry parameters and hematocrit were followed during 10 days in a 13 months old Arabian Oryx (Oryx leucoryx) during capture myopathy. An increase was found in bilirubin, creatine-kinase, alanine aminotransferase, and aspartate aminotransferase levels, but not in potassium level. Most of the parameters analyzed were the first given for this species.

Serological survey for bovine bacterial and viral pathogens in captive Arabian oryx (Oryx leucoryx Pallas, 1776).

Tests for antibodies to bovine bacterial and viral pathogens were conducted on 239 sera from 128 Arabian oryx (Oryx leucoryx) from seven locations (Taif, Riyadh and Mahazat as Said, Saudi Arabia; San Diego, United States of America [USA]; Shaumari, Jordan; Qatar; and Bahrain). No antibodies to Pasteurella multocida type E or epizootic haemorrhagic disease 1 virus were found. Antibodies to Brucella abortus, P. multocida type B, P. multocida type D, lumpy skin disease virus and Akabane virus were detected in 2, 1, 5, 2 and 1 animals, respectively. Evidence of P. multocida type A, Coxiella burnetti, Chlamydia psittaci and parainfluenza 3 virus was found in 3 herds (prevalence in the main herd [n = 78]: 8%), 3 herds (8%), 6 herds (7%) and 5 herds (15%), respectively. Evidence of antibodies against bluetongue virus was found in five oryx from the USA and in one oryx from the Taif herd. Antibody vaccinal titres against rinderpest virus (and the virus of peste des petits ruminants, due to cross-reactions) were found in almost all the herds. This is the first report of antibodies against B. abortus, C. burnetti, C. psittaci, parainfluenza 3 virus and Akabane virus in the genus Oryx.

Capripoxvirus disease in an Arabian oryx (Oryx leucoryx) from Saudi Arabia.

Lumpy skin disease caused by a capripoxvirus was observed in a captive-bred female Arabian oryx (Oryx leucoryx) at the National Wildlife Research Center, Taif, Saudi Arabia. Clinical signs included severe general depression with fever, anorexia, greater than 1,000 nodular cutaneous lesions and gradual recovery over 2 mo. The virus was found by electron microscopy and paired sera showed an increasing virus neutralization antibody titer against capripoxvirus. A serologic survey of the herd of 90 oryx showed a low prevalence (2%) of this infection. This report describes the first case of lumpy skin disease in an Arabian oryx.

Hematological and serum chemistry values for Arabian oryx (Oryx leucoryx).

Blood samples obtained from 73 captive Arabian oryx (Oryx leucoryx) were analyzed for hematology and serum chemistry values. Compared with other data from this animal RBC, WBC, bilirubin and ASAT values are lower, but glucose, urea and uric acid values are higher.

Robertsonian chromosome polymorphism in the Arabian oryx (Oryx leucoryx).

A Robertsonian translocation was found in a herd of Arabian oryx (Oryx leucoryx). The translocated chromosome, when analyzed by G-banding, seemed to involve the fusion of chromosomes 17 and 19. The results of C-banding suggested that the fused chromosome is dicentric. The translocation was traced back through two generations and occurred in a total of 8 of 62 animals in the herd.

The G- and C-banding karyotype of the Arabian oryx (Oryx leucoryx).

The chromosomes of the Arabian oryx (Oryx leucoryx) were investigated using GTG and CBG banding technique. Their banding patterns were compared to those of goat and cattle.