ABSTRACT
This study aimed to assess the protective effects of purslane polysaccharide (PP) on colonic impairments in mice exposed to cadmium (Cd). C57BL/6 mice were administered with PP (200-800 mg/kg/day) by gavage for 4 weeks after treatment with 100 mg·L-1 CdCl2. PP significantly reduced Cd accumulation in the colon tissue and promoted the excretion of Cd in the feces. PP could reduce the expression levels of inflammatory factors (tumor necrosis factor-α (TNF-α), interleukin-1ß (IL-1ß), and IL-6) and inhibit the activation of the TLR4/MyD88/NF-κB signaling pathway. In addition, the results of 16S rRNA analysis revealed that PP significantly increased the abundance of probiotics (Lactobacillus), while decreased the abundance of pathogenic bacteria (Lachnospiraceae_NK4A136_group). Following the augmentation of beneficial intestinal bacteria, the treatment with PP led to an increase in the levels of intestinal microbial metabolites, specifically short-chain fatty acids (SCFAs). The SCFAs are known for their anti-inflammatory properties, immune-regulatory effects, and promotion of intestinal barrier function. Additionally, the results suggested that PP effectively impeded the enterohepatic circulation by inhibiting the FXR-FGF15 axis in the intestines of Cd-exposed mice. In summary, PP mitigated the toxic effects of Cd by limiting its accumulation and suppressing inflammatory responses in colon.