Settling and Ovipositional Behavior of Bactericera cockerelli (Hemiptera: Triozidae) on Solanaceous Hosts Under Field and Laboratory Conditions.

Potato psyllid, Bactericera cockerelli (Sulc), is a seasonal insect pest in the Lower Rio Grande Valley of Texas, where it transmits the bacterial pathogen "Candidatus Liberibacter solanacearum" that causes zebra chip disease of potato. Studies were conducted to evaluate host preference of B. cockerelli adults for different plant species, and plant size and density. Settling and oviposition behavior of B. cockerelli was studied on its wild and cultivated solanaceous hosts, including potato, tomato, pepper, eggplant, and silverleaf nightshade, under both field and laboratory conditions. Naturally occurring B. cockerelli were used to evaluate host preference under open field conditions throughout the growing season. Settling and oviposition preference studies in the laboratory were conducted as cage-release experiments using pairs of plants, and observations were recorded over a 72-h period. Results of field trials indicated that naturally occurring B. cockerelli preferred potato and tomato equally for settling and oviposition, but settled on pepper, eggplant, and silverleaf nightshade only in the absence of potato and tomato. Under laboratory conditions, B. cockerelli adults preferred larger host plants, regardless of the species tested. Results also showed that movement of B. cockerelli was minimal after initial landing and settling behavior was influenced by host plant density. Lone plants attracted the most psyllids and can be used as sentinel plants to monitor B. cockerelli activity. Information from both field and laboratory studies demonstrated that not only host plant species determined host selection behavior of B. cockerelli adults, but also plant size and density.

Characterization of management and environmental factors associated with regional variations in potato zebra chip occurrence.

Potato zebra chip (ZC), caused by the bacterial pathogen 'Candidatus Liberibacter solanacearum', which is vectored by the potato psyllid (Bactericera cockerelli), has caused widespread damage to U.S. potato production ever since its first discovery in south Texas in 2000. To determine the influence of environmental factors and management practices on ZC occurrence, data on management and meteorological variables, field locations, and psyllid counts were collected over a 3-year period (2010 to 2012) from six locations across the central United States (south Texas to Nebraska). At these locations, ZC-symptomatic plants were counted in 26 fields from systematically established 20 m × 30 m plots around the field edges and field interiors. Mean numbers of symptomatic plants per plot were classified into two intensity classes (ZC ≤ 3 or ZC > 3) and subjected to discriminant function and logistic regression analyses to determine which factors best distinguish between the two ZC intensity classes. Of all the variables, location, planting date, and maximum temperature were found to be the most important in distinguishing between ZC intensity classes. These variables correctly classified 88.5% of the fields into either of the two ZC-intensity classes. Logistic regression analysis of the individual variables showed that location accounted for 90% of the variations, followed by planting date (86%) and maximum temperature (70%). There was a low but significant (r = -0.44983, P = 0.0211) negative correlation between counts of psyllids testing positive for pathogen and latitudinal locations, indicating a south-to-north declining trend in counts of psyllids testing positive for the pathogen. A similar declining trend also was observed in ZC occurrence (r = -0.499, P = 0.0094).

Seasonal population dynamics of the potato psyllid (Hemiptera: Triozidae) and its associated pathogen "Candidatus Liberibacter solanacearum" in potatoes in the southern great plains of North America.

The potato psyllid, Bactericera cockerelli (Sulc) (Hemiptera: Triozidae), and its associated pathogen "Candidatus Liberibacter solanacearum" (Ca. L. solanacearum), the putative causal agent of zebra chip (ZC) disease in potatoes (Solanum tuberosum L.), were sampled in commercial potato fields and untreated control plots for 3 yr in multiple locations in Texas, Kansas, Nebraska, and Colorado. Populations of the potato psyllid varied across years and across potato growing regions. However, the percentage of potato psyllids infected with Ca. L. solanacearum although variable across years, was consistently highest in the Lower Rio Grande Valley of Texas (LRGV), the reported overwintering location for this pest. The numbers of Ca. L. solanacearum-infected psyllids collected on field traps and large nymphs counted on leaf samples were both positively correlated with the final percentage of ZC in tubers. In the LRGV, where vector and disease pressure is the highest, population levels of immature life stages of the psyllid and percentage of ZC differed greatly between commercial and untreated fields. These results show that the pest management program that was used can be effective at controlling development of the psyllid and ultimately reducing the incidence of ZC.

Assessments of the Edge Effect in Intensity of Potato Zebra Chip Disease.

Zebra chip is a newly emerging potato disease which imparts dark colorations on fried chips, rendering them unmarketable. The disease is associated with the phloem-limited proteobacterium 'Candidatus Liberibacter solancearum', vectored by the potato psyllid Bactericera cockerelli. First reported from Mexico in the mid-1990s, the disease was observed for the first time in Texas in 2000 and is now prevalent in several potato-producing regions of the United States. In this study, we were interested in investigating whether there are edge effects in zebra chip intensity that can be assessed as a "foot print" of the associated insect vector. In 2009, we conducted studies in three fields in the Texas Panhandle in paired plots of 10 by 20 m around the field edges and 100 m infield in which symptomatic plants were counted just before harvest. The number of plot pairs (edge and infield) ranged from 15 to 18 depending on the size of the fields. In a separate study, temporal disease progress was assessed in two fields around the edges of the center-pivot circle in approximately 10-by-450-m areas. In 2010, the paired plot studies were repeated in 10 potato fields in Texas, Kansas, and Nebraska. Zebra chip intensity data from the paired-plot studies for both years were analyzed using the Wilcoxon's signed-rank test, a nonparametric equivalent of the classical (parametric) paired t test. In the 2009 study in all three fields, the edge plots had significantly greater zebra chip intensity than the infield plots (P < 0.05). Edge plots in the 2010 study also had greater zebra chip intensity in all fields and the differences were significant in the majority of fields (P < 0.05). In the diseases progress study in both fields, weekly zebra chip intensity on the edges reached its maximum after the third week of its first detection, and the disease progress curves were best fitted with the second-degree polynomial (quadratic) for both fields. The 2-year study clearly demonstrated that zebra chip intensity in potato fields was greater on the edges than in the infields. This finding has significant implications for psyllid management because greater emphasis in psyllid control strategy can be directed toward the edges for better results.

Spatial Patterns and Spread of Potato Zebra Chip Disease in the Texas Panhandle.

Zebra chip (ZC) is a disease that is affecting potato production in the southwestern United States and in other countries, and which has been linked to potato psyllids (Bactericera cockerelli) that harbor the bacterial plant pathogen 'Candidatus Liberibacter solanacearum'. Until recently, the epidemiology of ZC was unknown, motivating research to elucidate the spatial and temporal patterns of ZC infections in potato fields. Studies were performed in multiple commercial potato fields located in the Texas Panhandle, wherein locations of ZC-affected potato plants were georeferenced or counted within large plots and along belt transects consisting of contiguous 10-by-10-m quadrats. By employing distance- and area-based spatial statistical methods, it was determined that locations of ZC infections in potato fields departed from a completely spatially random pattern, instead appearing as clusters comprising infected plants situated in close proximity to one another, with clusters interspersed with numerous solitary infections. Disease progress curves of ZC clusters were generally well described by exponential growth and quadratic polynomial models. Numbers of ZC infections within disease clusters gradually increased over multiple weeks, with foliar disease symptoms first appearing during the tuber bulking stage. ZC infections were not found to be continuously present across fields, because many quadrats along belt transects contained zero or only a few infections while others had numerous infections. Consequently, the frequency of ZC infections within belt transect quadrats was well described by negative binomial and zero-inflated negative binomial distributions, in agreement with observed clustering of infections and distance-based spatial statistical results.

Movement of Bactericera cockerelli (Heteroptera: Psyllidae) in relation to potato canopy structure, and effects on potato tuber weights.

With the threat of new plant diseases on the increase, plant disease epidemiology requires research on pathogen vector movement. Here, releases were performed in planted potato fields of different ages and canopy structures, located in the Texas Panhandle, to evaluate the range of movement of the potato psyllid Bactericera cockerelli (Sulc.). This insect is a known causative agent of psyllid yellows disease, and is a vector of the reported etiological agents of zebra chip disease of potato, 'Candidatus Liberibacter solanacearum/psyllaurous'. Based on collections of B. cockerelli immatures along transects 9 m long radiating in four cardinal directions from release points, adult females dispersed considerable distances, regardless of plant age or canopy structure. Immature abundance declined along transects and were well described by linear and nonlinear models, but abundance patterns did not differ among the different planting dates and canopy structures. However, unequal immature abundance was detected among the four cardinal directions, with more immatures generally collected along transects to the north and west of release points, opposite of prevailing winds in the area at the time of release. Plots where B. cockerelli were released had significantly lower mean potato tuber weights than control plots with no B. cockerelli. However, few plots with B. cockerelli released in them had declining trends in tuber weights with increasing distance from release points.

Characterization and Epidemiological Significance of Potato Plants Grown from Seed Tubers Affected by Zebra Chip Disease.

An emerging disease of potato in the United States, known as "Zebra Chip" or "Zebra Complex" (ZC), is increasing in scope and threatens to spread further. Here, we report on studies performed to understand the role of tuberborne ZC in the epidemiology of this disease. Depending on variety, up to 44% of ZC-affected seed tubers (ZCST) were viable, producing hair sprouts and weak plants. Chip discoloration in progeny tubers of ZCST was more severe than those from ZC-asymptomatic seed tubers but varied depending on whether progeny tubers or foliage were positive or negative for 'Candidatus Liberibacter solanacearum'. A low percentage of greenhouse-grown plants produced by ZCST tested positive for 'Ca. Liberibacter'. No adult potato psyllids became infective after feeding upon these plants but they did acquire 'Ca. Liberibacter' from field-grown plants produced by ZCST. Plants with new ZC infections near plants produced by ZCST were not significantly different from healthy plants, whereas plants affected with ZC from infectious potato psyllids had significantly more ZC infections near either plants produced by ZCST or healthy plants. We conclude that, in areas where ZC is currently established, plants produced by ZCST do not significantly contribute to ZC incidence and spread within potato fields.