ABSTRACT
Live chicken egg embryos offer new opportunities for evaluation and continuous monitoring of tumour growth for in vivo studies compared to traditional rodent models. Here, we report the first use of surface enhanced Raman scattering (SERS) mapping and surface enhanced spatially offset Raman scattering (SESORS) for the detection and localisation of targeted gold nanoparticles in live chicken egg embryos bearing a glioblastoma tumour.
Subject(s)
Gold , Metal Nanoparticles , Spectrum Analysis, Raman , Animals , Spectrum Analysis, Raman/methods , Gold/chemistry , Chick Embryo , Metal Nanoparticles/chemistry , Glioblastoma/pathology , Glioblastoma/diagnostic imaging , Humans , Surface Properties , Disease Models, Animal , Cell Line, TumorABSTRACT
Driven by the longer lifespans of humans, particularly in Westernised societies, and the need to know more about 'healthy ageing', ageing mice are being used increasingly in scientific research. Many departments and institutes involved with ageing research have developed their own systems to determine intervention points for potential refinements and to identify humane end points. Several good systems are in use, but variations between them could contribute to poor reproducibility of the science achieved. Working with scientific and regulatory communities in the UK, we have reviewed the clinical signs observed in ageing mice and developed recommendations for enhanced monitoring, behaviour assessment, husbandry and veterinary interventions. We advocate that the default time point for enhanced monitoring should be 15 months of age, unless prior information is available. Importantly, the enhanced monitoring should cause no additional harms to the animals. Where a mouse strain is well characterised, the onset of age-related enhanced monitoring may be modified based on knowledge of the onset of an expected age-related clinical sign. In progeroid models where ageing is accelerated, enhanced monitoring may need to be brought forward. Information on the background strain must be considered, as it influences the onset of age-related clinical signs. The range of ageing models currently used means that there will be no 'one-size fits all' solution. Increased awareness of the issues will lead to more refined and consistent husbandry of ageing mice, and application of humane end points will help to reduce the numbers of animals maintained for longer than is scientifically justified.
Subject(s)
Aging , Animal Husbandry/standards , Animal Welfare/standards , Animals, Laboratory/physiology , Mice/physiology , Animals , United KingdomABSTRACT
Este é um relato de experiência profissional onde foi realizada uma revisão bibliográfica sobre instrumentação cirúrgica e a descrição da atuação de enfermeiros como instrumentadores cirúrgicos em um hospital de reabilitação em Brasília. Além disso, foi caracterizado o método 5S da Qualidade Total e descrito o seu uso para a realização da instrumentação cirúrgica, com a finalidade de criar mecanismos educativos para o treinamento dos enfermeiros...
Subject(s)
Humans , Operating Room Nursing , Surgical Instruments , SafetyABSTRACT
Estudo desenvolvido em um hospital de reabilitação para elaborar um sistema informatizado de materiais cirúrgicos. O sistema criado tem o objetivo de orientar e direcionar o preparo das cirurgias e possibilitar a troca de informações entre Centro Cirúrgico (CC), Central de Material e Esterilização (CME) e os cirurgiões e a equipe de enfermagem. Método: os materiais utilizados pelas equipes cirúrgicas foram revisados e listados pelos enfermeiros da CME e do CC e alimentaram um programa de software específico, denominado Sistema de Suporte para Procedimentos Cirúrgicos. Este trabalho contribuiu para organização de carrinhos cirúrgicos, salas de indução anestésica e operatória, planejamento e autonomia das condutas, educação continuada, treinamento e melhor operacionalização do serviço .