Tuning surface functionalization and collagen gel thickness to regulate cancer cell migration.

Cancer cells have a tremendous ability to sense and respond to extracellular matrix (ECM) stiffness, modulating invasion. The magnitude of the sensed stiffness can either promote or inhibit the migration of cancer cells out of the primary tumor into surrounding tissue. Work has been done on examining the role of stiffness in tuning cancer cell migration by controlling elastic modulus in the bulk. However, a powerful and complementary approach for controlling stiffness is to leverage interactions between stiff-soft (e.g. glass-hydrogel) interfaces. Unfortunately, most work in this area probes cells in 2D environments. Of the reports that probe 3D environments, none have assessed the role of mechanical linkage to the interface as a potential handle in controlling local stiffness and cell behavior. In this paper, we examine the migration of cancer cells embedded in a collagen fiber network between two flat plates. We examine the role of both surface attachment of the collagen network to the stiff interface as well as thickness (50-540 µm) of the collagen gel in driving collagen organization, cell morphology and cell migration. We find that surface attachment and thickness do not operate overlapping mechanisms, because they elicit different cell responses. While thickness and surface chemistry appear to control morphology, only thickness regulates collagen organization and cell migration speed. This suggests that surface attachment and thickness of the collagen gel control cell behavior through both collagen structure and local stiffness in confined fiber-forming networks.

Tunable Mechanical Metamaterials through Hybrid Kirigami Structures.

Inspired by the art of paper cutting, kirigami provides intriguing tools to create materials with unconventional mechanical and morphological responses. This behavior is appealing in multiple applications such as stretchable electronics and soft robotics and presents a tractable platform to study structure-property relationships in material systems. However, mechanical response is typically controlled through a single or fractal cut type patterned across an entire kirigami sheet, limiting deformation modes and tunability. Here we show how hybrid patterns of major and minor cuts creates new opportunities to introduce boundary conditions and non-prismatic beams to enable highly tunable mechanical responses. This hybrid approach reduces stiffness by a factor of ~30 while increasing ultimate strain by a factor of 2 (up to 750% strain) relative to single incision patterns. We present analytical models and generate general design criteria that is in excellent agreement with experimental data from nanoscopic to macroscopic systems. These hybrid kirigami materials create new opportunities for multifunctional materials and structures, which we demonstrate with stretchable kirigami conductors with nearly constant electrical resistance up to >400% strain and magnetoactive actuators with extremely rapid response (>10,000% strain s-1) and high, repeatable elongation (>300% strain).

Kirigami-Inspired Structures for Smart Adhesion.

Spatially controlled layouts of elasticity can provide enhanced adhesion over homogeneous systems. Emerging techniques in kirigami, where designed cuts in materials impart highly tunable stiffness and geometry, offer an intriguing approach to create well-defined layouts of prescribed elastic regions. Here, we show that kirigami-inspired structures at interfaces provide a new mechanism to spatially control and enhance adhesion strength while providing directional characteristics for smart interfaces. We use kirigami-inspired cuts to define stiff and compliant regions, where above a critical, material-defined length scale, bending rigidity and contact width can be tuned to enhance adhesive force capacity by a factor of ∼100 across a spatially patterned adhesive sheet. The directional nature of these designs also imparts anisotropic responses, where peeling in different directions results in anisotropic adhesion ratios of ∼10. Experimental results are well-supported by theoretical predictions in which the bending rigidity and contact width of kirigami-inspired structures and interconnects control the adhesive capacity. These new interfacial structures and design criteria provide diverse routes for advanced adhesive functionality, including spatially controlled systems, wearable kirigami-inspired electronics, and anisotropic kirigami-inspired bandages that enable strong adhesive capacity while maintaining easy release.