Development of a Modified Friedewald's Formula to Calculate Low-Density Lipoprotein in an Iranian Population.

Background: Elevated low-density lipoprotein cholesterol (LDL-C) is a significant risk factor for cardiovascular diseases. LDL-C can be directly measured using various methods, but this requires expensive equipment. Currently, clinical laboratories estimate LDL-C based on Friedewald's formula (FF). We aimed to develop a modified formula based on directly measured LDL-C (D-LDL-C) values in a large population in Southern Iran and compare the results with various other estimation formulas. Methods: The participants of this cross-sectional study were adults aged >18 years living in Southern Iran. Blood samples from 15,200 individuals were collected, and the measured lipid parameters were randomly divided into training (n=10,184) and validation (n=5,016) datasets. A new formula was developed using a linear regression model, and its accuracy was validated. Pearson's correlation and Cohen's kappa were used to determin the relationship between D-LDL-C and calculated LDL-C (C-LDL-C). Results: The developed formula for the estimation of LDL-C was 0.857 total cholesterol (TC)-0.915 high-density lipoprotein cholesterol (HDL-C)-0.115 triglycerides (TG). Based on our proposed formula, for TG<150 and TG≥150 mg/dL, there was a significant correlation between mean values of D-LDL-C and C-LDL-C (r=0.985 and r=0.974, respectively). Compared to other formulas, C-LDL-C obtained from the proposed formula had the highest correlation with D-LDL-C. The agreement between D-LDL-C and C-LDL-C for TC<200, 200-239, and ≥240 mg/dL was 80.8%, 63.2%, and 67.4%, respectively, indicating a higher level of agreement than other formulas. Conclusion: The new formula appears to be more accurate than FF when applied to the population of Southern Iran.

Climatic and topographic controls on glacial changes (1973-2020) in Shigar Basin, Central Karakoram, Northern Pakistan.

The Hindukush, Karakorum, and Himalaya (HKH) mountains are often referred to as the "Third Pole" because of high snow, being a major freshwater resource and early indicator of climate change. Therefore, research on the dynamics of glacier changes and their relationship with climate and topographic variability is essential for sustainable water resource management and adaptation strategies in Pakistan. In this contribution, we delineated 187 glaciers and examined these glacier changes in the Shigar Basin from 1973 to 2020 using Corona, Landsat Operational Land Imager/Enhanced Thematic Mapper Plus/Thematic Mapper/Multispectral Scanner System (OLI/ETM/TM/MSS), Alaska Satellite Facility (ASF), and Shuttle Radar Topography Mission Digital Elevation Model (SRTM DEM) imageries. The total glacier area decreased from 2796.31 ± 132 km2 in 1973 to 2756.27 ± 63 km2 in 2020 at an average rate of - 0.83 ± 0.03 km2yr-1. Specifically, during the period of 1990-2000, these glaciers shrank most heavily at an average rate of - 2.372 ± 0.08 km2yr-1. In contrast, an increased rate of 0.57 ± 0.02 km2yr-1 in total glacier area was observed during the recent decade (2010-2020). Moreover, the glaciers with gentle slopes retreated less heavily than the steep ones. There was reduction in glacier coverage and length for all slope classes, and a small reduction was observed with gentle slopes, while higher losses were observed on steep slope gradients. The transition of glaciers in the Shigar Basin may be attributed by the direct influence of glacier size and topographical characteristics. By comparing with climate records, our findings suggest that the overall reduction in glacier area from 1973 to 2020 was associated with declining precipitation (- 0.78 mmm/year) and rising temperature (0.045 °C/year) trends in the region, and glacier advances in recent decade (2010-2020) were likely to be driven by increased winter and autumn precipitation.

The effect of topical olive oil dressing on the healing of grade 1 and 2 diabetic foot ulcers: An assessor-blind randomized controlled trial in type 2 diabetes patients.

BACKGROUND AND AIMS: We aimed to compare the effect of topical olive oil dressing plus standard care with standard care alone on the treatment of grade 1 and 2 diabetic foot ulcers (DFUs) in type 2 diabetes mellitus (T2DM) patients. METHODS: This assessor-blind randomized controlled trial included 60 T2DM patients with DFU referred to the Diabetes Clinic of Shahid Mohammadi Hospital, Bandar Abbas, Iran, from February 21 to August 22, 2017. Patients were randomly assigned to intervention (n = 30) and control (n = 30) groups. The intervention group received standard care, including wound irrigation with normal saline and oral antibiotics plus daily topical olive oil dressing for four weeks, and the control group only received standard care. The wound healing assessment scale (wound degree, color, drainage, and surrounding tissue healing) was recorded weekly and the total wound status was determined at the end of the study. RESULTS: Treatment with olive oil led to significantly higher scores of ulcer degree, color, drainage, and surrounding tissue healing at weeks one, two, three and four in the olive oil group than in the control group (P < 0.001). Also, the total wound status score was higher in the olive oil group compared to the control group (P < 0.001). The proportions of completely healed, partially healed, and unhealed wounds were 76.6%, 23.3% and 0% in the intervention group, and 0%, 93.3% and 6.7% in the control group, respectively. CONCLUSIONS: Topical olive oil dressing promoted the healing of DFU and it can be recommended as a safe and effective treatment in this regard. TRIAL REGISTRATION: Iranian Registry of Clinical Trials (IRCT), IRCT20150607022585N4. Registered 05/12/2018. Retrospectively registered, https://www.irct.ir/trial/19460.