ABSTRACT
Objective: To explore the present situation and epidemiological characteristics of pesticide poisoning in Chengdu City from 2012 to 2021, and to provide scientific evidence for further prevention and control. Methods: In January 2022, the pesticide poisoning report cards of Chengdu City from 2012 to 2021 were collected from the China Disease Control and Prevention Information System. The data of the report card was reorganized and the distribution characteristics of pesticide poisoning such as time, region, gender, age and pesticide types were analyzed. Results: 14326 cases of pesticide poisoning were reported in Chengdu City from 2012 to 2021, 651 deaths, and the fatality rate was 4.54%. The cases of productive pesticide poisoning and unproductive pesticide poisoning were 504 and 13822, respectively. The fatality rates of productive and unproductive pesticide poisoning were 1.39% and 4.66%, which were significant different (χ(2)=11.99, P=0.001). The highest reported cases of pesticide poisoning was in 2013 (1779) and the lowest in 2021 (1047). The number of reported cases showed a downward trend year by year (t=-12.30, P<0.001), and the fatality rates also showed a downward trend year by year (χ(2)(trend)=25.48, P<0.001). The fluctuation range of unproductive pesticide poisoning cases in each month of the year was small, and the productive pesticide poisoning mainly occurred from May to August. The regions with the largest number of reported poisoning cases were Pengzhou (1620), Jianyang (1393), Jintang (1266) and Qionglai (1158). The high incidence of poisoning was among 25-54 years old (50.21%, 7193/14326). The fatality rate in the age group 75-96 years old was the highest (8.98%, 95/1058), and the fatality rates increased gradually with age (χ(2)(trend)=186.03, P<0.001). The pesticides causing poisoning were mainly insecticide (43.86%, 6284/14326) and herbicides (35.75%, 5121/14326). Herbicides paraquat had the highest fatality rate (9.54%, 286/2998) . Conclusion: Pesticide poisoning in Chengdu City is mainly unproductive poisoning. Health education should be carried out for key areas and people, and the control of highly toxic pesticides such as insecticide and herbicides should be strengthened.
Subject(s)
Herbicides , Insecticides , Pesticides , Poisoning , Humans , Aged , Aged, 80 and over , Adult , Middle Aged , Paraquat , Cities , Poisoning/epidemiologyABSTRACT
Objective: To explore theepidemiological characteristics and analyse the survival of pneumoconiosis in Chengdu from 2011 to 2021, providing scientific evidence for further prevention and control. Methods: In April 2022, the pneumoconiosis report card of Chengdu from 2012 to 2021 and survival data were collected from the China Disease Control and prevention information system and the occupational pneumoconiosis follow-up survey project.The data of the report card was reorganized and analyzed by R4.4.0 software. Chi-square test was used to compare the case composition ratio between groups. The annual trend of the number of new pneumo coniosis cases was analyzed by linear regression model Kaplan-Meier method was used for survival analysis and log-rank test was used for comparison of survival curves between groups. Results: 816 cases of pneumoconiosis were reported in Chengdu from 2011 to 2021, including 522 cases of stage â (63.97%, 522/816), 148 cases of stage â ¡ (18.14%, 148/816) and 146 cases of stage â ¢ pneumoconiosis (17.89146/816) ; There were 596 cases of silicosis (73.04%, 596/816), 143 cases of coal worker's pneumoconiosis (17.52%, 143/816). New onset pneumoconiosis was mainly male (810 cases, 99.26%). The median age of diagnosis and the 25th and 75th percentile were 63 (51-71) years old, the median length of dust exposure were 12.00 (5.92-28.00) years, and the types of work were mainly rock drillers (24.63%, 201/816), tunneling workers (19.36%, 158/816) and coal miners (13.60%, 111/816). Among 816 cases of new pneumoconiosis, 35 cases were lost to follow-up (4.29%, 35/816), 605 cases survived and 176 cases died. The 5-year survival rate was 78.6% and the 10-year survival rate was 65.8%. Conclusion: The number of pneumoconiosis reported in Chengdu was relatively stable in recent years. The main type of pneumoconiosis was silicosis, which was concentrated in regions, industries and types of work. We should continue to strengthen the prevention and treatment of pneumoconiosis.
Subject(s)
Anthracosis , Cardiology , Pneumoconiosis , Silicosis , Humans , Male , Middle Aged , Aged , Female , Pneumoconiosis/epidemiology , Silicosis/epidemiology , Anthracosis/epidemiology , Survival AnalysisABSTRACT
Atomic clocks, which lock the frequency of an oscillator to the extremely stable quantized energy levels of atoms, are essential for navigation applications such as deep space exploration1 and global navigation satellite systems2, and are useful tools with which to address questions in fundamental physics3-6. Such satellite systems use precise measurement of signal propagation times determined by atomic clocks, together with propagation speed, to calculate position. Although space atomic clocks with low instability are an enabling technology for global navigation, they have not yet been applied to deep space navigation and have seen only limited application to space-based fundamental physics, owing to performance constraints imposed by the rigours of space operation7. Methods of electromagnetically trapping and cooling ions have revolutionized atomic clock performance8-13. Terrestrial trapped-ion clocks operating in the optical domain have achieved orders-of-magnitude improvements in performance over their predecessors and have become a key component in national metrology laboratory research programmes13, but transporting this new technology into space has remained challenging. Here we show the results from a trapped-ion atomic clock operating in space. On the ground, NASA's Deep Space Atomic Clock demonstrated a short-term fractional frequency stability of 1.5 × 10-13/τ1/2 (where τ is the averaging time)14. Launched in 2019, the clock has operated for more than 12 months in space and demonstrated there a long-term stability of 3 × 10-15 at 23 days (no drift removal), and an estimated drift of 3.0(0.7) × 10-16 per day. Each of these exceeds current space clock performance by up to an order of magnitude15-17. The Deep Space Atomic Clock is particularly amenable to the space environment because of its low sensitivity to variations in radiation, temperature and magnetic fields. This level of space clock performance will enable one-way navigation in which signal delay times are measured in situ, making near-real-time navigation of deep space probes possible18.
ABSTRACT
To evaluate the clinical value of emergency endovascular embolization in the interventional treatment for oral hemorrhage caused by carcinoma, 32 patients with oral hemorrhage caused by carcinoma, who received emergency endovascular embolization due to unsatisfactory hemostatic effect of conventional conservative treatment in the First Affiliated Hospital of Zhengzhou University from January 2014 to December 2019, were included in this study and their clinical data, laboratory data and imaging information were retrospectively analyzed. There were 16 males and 16 females, aged (60.6±13.6) years (34-88 years). Technical successful rate of emergency endovascular embolization, immediate successful rate of controlling hemorrhage, blood pressure before and after operation, hemoglobin before and after operation, postoperative complications and recurrence rate of oral hemorrhage were statistically analyzed. Results showed that technical successful rate of operation and immediate successful rate of controlling oral hemorrhage are both 100% (32/32). Recurrent oral hemorrhage occurred in 4 patients (13%). The hemorrhagic shock symptoms of all patients were significantly improved after interventional therapy. After operation, local swelling happened in 34% (11/32) patients and intermittent local pain happened in 22% (7/32) within 24 hours; the swelling and the pain gradually disappeared from 2nd to 5th days. Mild complications of transient fever happened in 9% (3/32) patients and disappeared spontaneously in the short term. No serious complications such as blindness, cerebrovascular accident or central nervous system disturbance occurred in all patients after operations. During the whole follow-up period (1 to 12 months), a total of 8 patients died. The causes of death were progression and metastasis of carcinoma (n=4), heart failure (n=2), severe pneumonia (n=1) and respiratory failure caused by recurrent oral hemorrhage (n=1). Owing to the remarkable short-term curative effect, repeatable operation, low recurrence rate of oral hemorrhage and low incidence of complications, emergency endovascular embolization can be used in the clinical therapy and application of oral hemorrhage caused by carcinoma.
Subject(s)
Carcinoma , Embolization, Therapeutic , Aged , Female , Humans , Male , Middle Aged , Neoplasm Recurrence, Local , Oral Hemorrhage , Retrospective Studies , Treatment OutcomeABSTRACT
Objective: A Meta-analysis was used to evaluate the high frequency hearing loss detection rate, its area and time distributions in occupational noise exposed workers in China to provide evidence for the prevention and control of occupational noise-induced deafness. Methods: From February to April 2020, the number of hearing tests and the number of binaural high frequency average hearing threshold ≥40 dB of workers exposed to noise in key occupational disease monitoring and occupational health risk assessment project in China was collected from China National Knowledge Infrastructure (CNKI) , VIP database and PubMed database. The retrieval time was set from the establishment of the database to February 28, 2020. The R 3.6.2 software was used to calculate the pooled high frequency hearing loss detection rate. Results: A total of 28 studies, included 34 data and 1259571 occupational noise exposed workers, were enrolled. The articles covered 16 provinces in 2015-2018. The pooled high frequency hearing loss detection rate in occupational noise exposed workers was 9.45% (95%CI: 7.64%-11.64%) . The high frequency hearing loss detection rate (10.54%) was higher in 2018 than other years (2017: 10.17%, 2016: 9.21%, 2015: 7.88%) . The high frequency hearing loss detection rate (12.23%) was higher in central China than in eastern (10.21%) , western (7.91%) and north-eastern (5.21%) China. Conclusion: The overall high frequency hearing loss detection rate in occupational noise exposed workers is generally high in China. It is important to strengthen the hearing protection in occupational noise exposed workers to facilitate the occupational noise-induced deafness prevention and control in China.
Subject(s)
Hearing Loss, Noise-Induced , Noise, Occupational , Occupational Diseases , Occupational Exposure , China/epidemiology , Hearing Loss, High-Frequency , Hearing Loss, Noise-Induced/diagnosis , Hearing Loss, Noise-Induced/epidemiology , Humans , Noise, Occupational/adverse effects , Occupational Diseases/diagnosis , Occupational Diseases/epidemiologyABSTRACT
Objective: To evaluate the effect of smoking cessation project run by the Central Subsidy Smoking Cessation Clinic and to explore the related influencing factors on smoking cessation, in order to improve related services and provide better guidance to these smoking cessation clinics. Methods: Practitioners who had been trained to run smoking cessation projects were recruited to conduct face-to-face interview with the smokers. Questionnaires were completed to provide information on related psychological, social and behavioral issues. In these clinics, medications were provided to the patients by the health care takers in the clinic. One month after the first visit, smoking cessation rate (self-reported, 7-day point prevalence abstinence rate at 30-day follow-up) was counted. Results: The overall smoking cessation rate (self-reported, 7-day point prevalence abstinence rate at 30-day follow-up) appeared as 34.1%. Results from the multivariate logistic regression showed that patients over the age of 60 were the ones most likely to quit smoking. Smokers who showed higher possibility of quitting would include those: not on the daily base, intend to quit within 30 days, with other diseases, or taking varenicline and bupropion. Factors as unemployment, longer history of smoking, bigger quantity of cigarettes consumption per day, dependence on nicotine and urgency on taking up the first cigarette in the early morning etc., were related to the less likelihood of giving up smoking. However, histories of cessation did not seem to affect the possibility of quitting. Conclusions: Data from self-reported 7-day point prevalence abstinence at 30-day follow-up study showed that the smoking cessation intervention programs run by the central subsidy smoking cessation clinic project had been effectively implemented. Advocacy on quit smoking at early stage seemed to have better outcomes, thus should be called for. Since medications as varenicline tartrate and bupropion hydrochloride can increase the possibility of stop smoking, we would suggest that all the hospitals which are with smoking cessation clinics be equipped with these medicines. Professional assistance provided by practitioners is of key importance to help overcome the withdrawal symptoms during the periods of cessation, on these smokers.
Subject(s)
Outpatients/psychology , Smoking Cessation/statistics & numerical data , Bupropion/therapeutic use , China , Follow-Up Studies , Humans , Outpatients/statistics & numerical data , Program Evaluation , Surveys and Questionnaires , Varenicline/therapeutic useABSTRACT
Objective: To assess the safety and efficacy of transjugular intrahepatic portosystemic shunt (TIPS) combined with AngioJet mechanical thrombectomy for liver cirrhosis with extensive portal vein thrombosis. Methods: From March 2018 to April 2019, a total of 11 patients with liver cirrhosis and extensive portal vein thrombosis were treated by TIPS combined with AngioJet mechanical thrombectomy, including 6 males and 5 females, with the age of 37-71 (46±9) years old, 3 cases of Child-Pugh grade A, 8 cases of grade B and 0 cases of grade C. The intraoperative immediate thrombus clearance rate, perioperative complication rate, postoperative thrombus recurrence rate, rebleeding rate, the incidence of hepatic encephalopathy and the rate of stent patency of all cases were collected and analyzed. Results: All the patients were treated successfully. The immediate complete thrombus clearance (grade â ¢) rate of portal vein trunk was 9/11, and grade â ¡ was 2/11, The average dose of urokinase was 30-60 (40±5) ten thousand U, slight puncture point bleeding occurred in 3 cases, and recurrence of PVT in portal vein trunk occurred in 1 case with â ¡ grade clearance rate after operation, rebleeding occurred in 1 case, hepatic encephalopathy occurred in 2 cases, the primary patency rate of stents was 9 cases. Conclusion: TIPS combined with AngioJet mechanical thrombectomy can treat the liver cirrhosis with extensive portal vein thrombosis effectively and safely, and postoperative portal vein patency rate and intrahepatic shunt patency rate are high.
Subject(s)
Portasystemic Shunt, Transjugular Intrahepatic , Thrombosis , Adult , Aged , Female , Humans , Liver Cirrhosis , Male , Middle Aged , Portal Vein , Thrombectomy , Treatment OutcomeSubject(s)
Coronavirus Infections , Lung Neoplasms , Lung , Pandemics , Pneumonia, Viral , Betacoronavirus , COVID-19 , Coronavirus Infections/pathology , Humans , Lung/pathology , Lung/virology , Lung Neoplasms/pathology , Lung Neoplasms/surgery , Lung Neoplasms/virology , Pneumonia, Viral/pathology , SARS-CoV-2ABSTRACT
Objective: To assess the feasibility and value of real-time image fusion technique guiding the procedure of transjugular intrahepatic portosystemic shunt(TIPS). Methods: From July 2017 to May 2018,a total of 48 consecutive patients complicated by portal venous hypertension due to cirrhosis who underwent TIPS were prospectively allocated into two groups that 27 cases underwent normal TIPS and 21 cases underwent image fusion guided TIPS. There were 25 males and 23 females with a mean age of 29-74(51±10) years. The differences of portal vein(PV) between image fusion angiographyand digital subtraction angiography(DSA), and the times of puncture PV, X-ray exposure dose and exposure time and contrast agent amount of all cases were collected and analyzed. Results: The longitudinal and traverse difference of PV between image fusion angiography and DSA were 1.7-2.5(2.1±0.2) mm and 0.9-1.8(1.4±0.3) mm, respectively.The times of puncture PV, X-ray exposure time and dose, and contrast agent amount between normal TIPS group and image fusion guided TIPS group were 1-7(3.8±0.6) times vs 1-3(2.0±0.6) times, 41-63(53±8)min vs 27-42(35±5) min, 513-787(644±96) mGy vs 357-524(423±59) mGy,102-196(151±23) ml vs 87-145(105±14) ml(all P<0.05), respectively. Conclusions: There are minor differences between image fusion angiography of PV and DSA. Real-time image fusion guided TIPS is feasible and valuable to reduce intraprocedural X-ray exposure time and dose and contrast agent amount of TIPS.
Subject(s)
Hypertension, Portal , Portasystemic Shunt, Transjugular Intrahepatic , Adult , Aged , Female , Humans , Liver Cirrhosis , Male , Middle Aged , Portal Vein , Retrospective Studies , Treatment Outcome , Young AdultABSTRACT
Objective: To explore the related influencing factors of high frequency hearing loss (HFHL) in workers exposed to noise and establish a prediction nomogram for HFHL. Methods: A total of 822 workers exposed to noise from 46 enterprises were included. A questionnaire survey and a pure-tone hearing test were conducted for the workers. The data of noise level of the workers exposed was also collected. After single factor analysis of related influencing factors, the multivariate Logistic regression analysis was performed to identify the final independent influencing factors of HFHL. Finally, a nomogram model was established by R software to achieve individual prediction of HFHL. Results: Among the 822 workers exposed to noise, 166 (20.2%) workers had HFHL. In multivariate Logistic regression analysis, increasing age, men, increasing wearing earphone time, less wearing earplugs, and high noise level were the independent risk factors for HFHL. The C-index of the nomogram model for predicting HFHL was 0.834 (95%CI: 0.748~0.903) . The area under the predictive power curve of nomogram model was 0.834 (95%CI: 0.799~0.869, P<0.001) . Conclusion: Age, sex, wearing earphone time, wearing earplugs, and noise level are independent influence factors for HFHL. The nomogram model is successfully established as a accurate and visible tool for individually predicting the HFHL risk in workers exposed to noise.
Subject(s)
Hearing Loss, High-Frequency , Hearing Loss, Noise-Induced , Noise, Occupational/adverse effects , Nomograms , Occupational Exposure , Ear Protective Devices , Humans , Male , Occupational DiseasesABSTRACT
WHAT IS KNOWN AND OBJECTIVE: Essential hypertension (EH) is a common disease exhibiting large individual difference in occurrence, development and treatment response. Genetic factors are implicated in the development and progression of EH. This study aimed to explore the association between NPR3 single nucleotide polymorphism rs2270915 (A/G, Asn521Asp) and the risk of EH in a Chinese Han population by a case-control study. METHODS: The study was a single-centre, case-control trial, in which a total of 287 EH patients and 289 age- and sex-matched healthy controls were enrolled. The inclusion criteria were as follows: Han Chinese origin, male or female patients, systolic blood pressure (SBP) ≥140 mm Hg and/or diastolic blood pressure (DBP) ≥90 mm Hg. The healthy controls were subjects without histories of cardiovascular or cerebrovascular diseases. NPR3 rs2270915 polymorphism was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). In addition, primary human umbilical vein endothelial cells (HUVECs) were isolated from 19 fresh human umbilical cords and cultured. Atrial natriuretic peptide (ANP) concentration in cell medium was determined by enzyme-linked immunosorbent assay (ELISA). NPR3 mRNA expression was determined by real-time semi-quantitative PCR. RESULTS AND DISCUSSION: No significant difference in genotype distribution of NPR3 rs2270915 polymorphism was observed between cases and controls (P>.05). Patients carrying the rs2270915 G allele showed decreased SBP, and the difference was marginal. As compared with cells carrying the rs2270915 AA genotype, those with the AG genotype showed significantly lower NPR3 mRNA expression levels (P<.05) and lower medium ANP concentration (P<.001). WHAT IS NEW AND CONCLUSION: This study suggested that NPR3 rs2270915 polymorphism was associated with decreased SBP level marginally in EH patients in a Chinese Han population, and the polymorphism may function through decreasing NPR3 mRNA expression and ANP level.
Subject(s)
Atrial Natriuretic Factor/metabolism , Blood Pressure/genetics , Essential Hypertension/genetics , Receptors, Atrial Natriuretic Factor/genetics , Aged , Aged, 80 and over , Alleles , Asian People/genetics , Case-Control Studies , Enzyme-Linked Immunosorbent Assay , Female , Genetic Predisposition to Disease , Genotype , Human Umbilical Vein Endothelial Cells , Humans , Male , Middle Aged , Polymorphism, Single Nucleotide , RNA, Messenger/metabolism , Real-Time Polymerase Chain ReactionABSTRACT
Appropriate antimicrobial therapy poses one of the greatest challenges during the management of a septic patient in the intensive care unit (ICU). Acute renal failure (ARF) is a common complication of sepsis and often occurs as a component of multiple organ dysfunction syndrome. Continuous renal replacement therapy (CRRT) is increasingly used as an effective extracorporeal blood purification therapy in this critically ill patient population. Available data demonstrate that sepsis, ARF and different modalities of CRRT may have profound effects on the pharmacokinetics and pharmacodynamics of various antimicrobial agents used in the ICU. Guidelines for antimicrobial prescription which will fit the individual patient undergoing a particular method of treatment are still unavailable. Understanding the principles of drug removal by CRRT and pharmacokinetics of various agents can help to modify the drug dosage and dosing intervals for individualized therapy. Meanwhile, monitoring the drug serum concentration is still mandatory whenever clinically feasible.
Subject(s)
Acute Kidney Injury/therapy , Anti-Infective Agents/administration & dosage , Anti-Infective Agents/pharmacokinetics , Renal Replacement Therapy , Acute Kidney Injury/epidemiology , Acute Kidney Injury/etiology , Anti-Infective Agents/analysis , Critical Illness , Dose-Response Relationship, Drug , Humans , Intensive Care Units , Multiple Organ Failure/epidemiology , Multiple Organ Failure/etiology , Multiple Organ Failure/therapy , Renal Dialysis , Renal Replacement Therapy/methods , Renal Replacement Therapy/trends , Sepsis/complications , Sepsis/drug therapy , Sepsis/epidemiology , Sepsis/physiopathologyABSTRACT
Dislocation of peritoneal dialysis catheters is one of the major causes of technique failure. We evaluated 701 Vicenza catheters, implanted since 1985 in 365 males, mean age 53 +/- 16 yrs, range 24 - 87, and 336 females, mean age 51 +/- 17 yrs, range 21 - 82. The Vicenza catheter is defined "short" since it consists of a classic straight double cuff PD catheter having however an inner segment (the portion located in the peritoneal cavity) much shorter than any other type of catheter. It is implanted in the lower abdomen, just a few centimeters above the pubis. The analysis of our results obtained in a large PD population displayed good device survival at 2 and 5 years (94.3% and 91.5% respectively), a low dislocation rate (4%) and an exit-site infection rate similar to other double cuffed catheters. There was no selection of patients receiving this catheter since from 1985 we have used this catheter in every incident patient. Due to its lower implantation site this catheter demonstrates excellent wearability and good body image acceptance.
Subject(s)
Catheters, Indwelling , Peritoneal Dialysis/instrumentation , Adult , Aged , Aged, 80 and over , Catheters, Indwelling/adverse effects , Device Removal , Equipment Design , Equipment Failure , Female , Humans , Male , Middle Aged , Patient Satisfaction , Survival AnalysisABSTRACT
A yeast (Saccharomyces cerevisiae) secretory expression vector containing PRO3 gene was constructed (pCBy310). beta HCG(Human choriogonadotropin beta subunit)-cDNA was inserted into pCBy310 to form a recombinant plasmid pCBy314. Since yeast proline auxotroph will not survive in rich medium (YPD), YPD could be used as a selection pressure, and pCBy314 could be maintained mitotically stable in transformants of yeast Pro3- auxotroph (MB299-7A) in rich medium. At an improved, yet not optimized cultural condition, the expression of beta HCG in culture medium was 650 micrograms/L. Our results showed not only that YPD could be used as a selection medium, but also that yeast grew better in YPD so as to increase the gene expression product, and that the component of YPD was simple and cheap. Our data suggested that PRO genes might be used widely in constructing vectors to clone and express foreign genes in yeast so that rich medium can be used as a selection pressure for direct selection.
Subject(s)
DNA, Complementary/genetics , DNA, Fungal/genetics , Genetic Vectors , Saccharomyces cerevisiae/genetics , Chorionic Gonadotropin, beta Subunit, Human/genetics , Transformation, GeneticABSTRACT
OBJECTIVE: To establish a new technique for three-dimensional surface reconstruction of teeth (dental surface reconstruction), and apply it to investigate impacted teeth in bone before operation. METHODS: On the basis of three-dimensional surface reconstruction of teeth and jaws, the dental surface reconstruction is obtained with the technique of shaded surface display (SSD), making transparent bone around. This technique is applied to investigate 9 cases of supernumerary and impacted teeth. RESULTS: Dental surface reconstruction image can present the dental surface image including crown, root, neck and root bifurcation in three-dimensional way, without presenting the image of other tissue around. In 4 cases, the abnormal configuration, labial or palatal location, and relation with dentition in anterior maxilla of 5 supernumerary were shown. In 1 case, the shape, location, eruption orientation and the angle of orthodontic distraction of 1 impacted permanent canine were displayed. In 3 cases, the shape, location, and extraction resistance of 6 impacted wisdom teeth were revealed. In 1 case, the configuration, distance to adjacent molar of 1 impacted wisdom tooth, which is a transposition tooth in maxillary sinus, were also demonstrated. CONCLUSION: The dental surface reconstruction is a new technique to display three-dimensional surface configuration of teeth. This technique can be applied to examine impacted and supernumerary teeth before surgical and orthodontic treatment.
Subject(s)
Tomography, Spiral Computed/methods , Tooth, Impacted/diagnostic imaging , Tooth, Supernumerary/diagnostic imaging , Adult , Child , Female , Humans , Imaging, Three-Dimensional , MaleABSTRACT
Electrophoretic karyotype of yeast strain A364a was obtained by pulsed field gel electrophoresis and the position of chromosome V on such karyotype was determined by means of dot hybridization with chromosome V-specific probe URA3. By cloning partially digested BamHI fragments of this chromosome DNA into integrative vector Yip5, a gene library specific to this chromosome was constructed. The number of the recombinants was much more than theoretically required. After screening probe-homologous fragments from this library and analysing such fragments with restriction enzymes BamHI, EcoRI, HindIII, PstI, and SalI, a fine physical map covering about 9.4% of A364a chromosome V (which was estimated as 620kb) was constructed. Further colony hybridization with boundary clones will enable us to "walk" throughout the whole chromosome.
Subject(s)
Chromosome Mapping , Chromosomes, Fungal , Saccharomyces cerevisiae/genetics , Chromosomes, Artificial, YeastABSTRACT
The title complexes were synthesized via reaction of iron nitrate, cobalt acetate and copper acetate with the didentate O,N-donor Schiff bases in methanol solution. The electronic and infrared spectra of these complexes were measured and the major IR bands were assigned.
Subject(s)
Cobalt/chemistry , Copper/chemistry , Iron/chemistry , Organometallic Compounds/chemistry , Organometallic Compounds/chemical synthesis , Electrons , Spectrophotometry, InfraredABSTRACT
The donor yeast strain YAC23 containing a 340 kb human genomic DNA fragment in YAC (Yeast Artificial Chromosome) was mated with recipient strain YLB504 by means of improved kar cross and the condidate YACductants were assayed by PCR and the amplification band of 404 bp indicated that they were the same as those of recipient strains (i.e. MAT alpha). Further analysis of the electrokaryotype of the candidate YACductants by PFGE (Pulsed Field Gel Electrophoresis) confirmed that the YACs had succeeded in entering into the recipient cells. The YACs had thus completed the process of transferring from one host to the other.
Subject(s)
Chromosomes, Artificial, Yeast/genetics , Gene Transfer Techniques , Crossing Over, Genetic , Genomic Library , HumansABSTRACT
We introduce a channel selection method for atmospheric remote-sensing problems described by a Fredholm integral equation of the first kind. Whether one set of channels (CH) is more suitable than another (CH') can be judged by whether (1) the degree of predominance (DP) value of CH is larger than that of CH', i.e., if the number of channels is the same and (2) the number of channels of CH is more than that of CH', if the DP values of both are acceptable. One can calculate the DP of the unknown function f (y) for a set of remote-sensing channels by DP = [1 + (RfÌ(a)(2) - 1) R(d)(2)](-1/2), RfÌ(a)(2) = R(c)(2)[ R(b)(2) + R(a)(2)(1 + R(b)(2))], where R(a), R(b), R(c), and (1 - R(d)(2))(1/2) of this channel set represent the influences on the ability to recover the unknown function caused by various measurement errors, the noise parameter, the relativity of the kernel functions, and the blindness of remote sensing means, respectively. Our channel selection method can be simplified to a conventional method when there are no differences in the relative measurement errors, no blind components of the unknown function and no noise parameters in the kernel function.