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1.
Animal ; 11(2): 295-305, 2017 Feb.
Article in English | MEDLINE | ID: mdl-27452785

ABSTRACT

An investigation of stillbirth and early neonatal lamb mortality was conducted in sheep flocks in Norway. Knowledge of actual causes of death are important to aid the interpretation of results obtained during studies assessing the risk factors for lamb mortality, and when tailoring preventive measures at the flock, ewe and individual lamb level. This paper reports on the postmortem findings in 270 liveborn lambs that died during the first 5 days after birth. The lambs were from 17 flocks in six counties. A total of 27% died within 3 h after birth, 41% within 24 h and 80% within 2 days. Most lambs (62%) were from triplet or higher order litters. In 81% of twin and larger litters, only one lamb died. The most frequently identified cause of neonatal death was infectious disease (n=97, 36%); 48% (n=47) of these died from septicaemia, 25% (n=24) from pneumonia, 22% (n=21) from gastrointestinal infections and 5% (n=5) from other infections. Escherichia coli accounted for 65% of the septicaemic cases, and were the most common causal agent obtained from all cases of infection (41%). In total, 14% of neonatal deaths resulted from infection by this bacterium. Traumatic lesions were the primary cause of death in 20% (n=53) of the lambs. A total of 46% of these died within 3 h after birth and 66% within 24 h. Severe congenital malformations were found in 10% (n=27) of the lambs, whereas starvation with no concurrent lesions was the cause of death in 6% (n=17). In 16% (n=43) of the lambs, no specific cause of death was identified, lambs from triplet and higher order litters being overrepresented among these cases. In this study, the main causes of neonatal lamb mortality were infection and traumatic lesions. Most neonatal deaths occurred shortly after birth, suggesting that events related to lambing and the immediate post-lambing period are critical for lamb survival.


Subject(s)
Animals, Newborn , Sheep Diseases/pathology , Animals , Congenital Abnormalities/pathology , Congenital Abnormalities/veterinary , Female , Norway , Pregnancy , Risk Factors , Sheep , Sheep Diseases/mortality , Starvation/veterinary , Stillbirth/veterinary
2.
J Appl Microbiol ; 113(6): 1389-95, 2012 Dec.
Article in English | MEDLINE | ID: mdl-22984812

ABSTRACT

AIMS: To isolate Bacillus anthracis from cattle carcass burial sites from high-risk districts in Zimbabwe. METHODS AND RESULTS: Soil samples were collected from carcass burial sites from seven areas, including two national game parks. Samples were collected from top 5-10 cm, and for spore extraction, 25 g of soil was suspended in sterile distilled water overnight. Supernatants were filtered through 0.45-µm pore cellulose nitrate, deposits suspended in 5 ml phosphate-buffered saline, aliquoted and heated at temperature regimen of 65, 70, 75 and 80 °C for 15 min. Samples were plated onto PLET agar. B. anthracis isolates were identified using growth morphology and PCR detecting pXO1 and pXO2 virulence plasmids. From samples heated at 75 °C for 15 min, B. anthracis were isolated from 9 of 81 (11.1%) soil samples representing five of the seven sampled areas. CONCLUSIONS: We isolated B. anthracis from soil collected from carcass burial sites. PCR targeting virulence plasmids provided a rapid confirmation of B. anthracis. SIGNIFICANCE AND IMPACT OF THE STUDY: The positive isolation indicated that some carcass burial sites may retain viable spores for at least 12 months after the previous outbreak, which suggests that they may be important sources of B. anthracis and new disease outbreaks.


Subject(s)
Bacillus anthracis/isolation & purification , Soil Microbiology , Spores, Bacterial/isolation & purification , Agar , Animals , Bacillus anthracis/genetics , Bacillus anthracis/growth & development , Cattle , Plasmids , Polymerase Chain Reaction , Zimbabwe
3.
Vet Rec ; 153(8): 231-5, 2003 Aug 23.
Article in English | MEDLINE | ID: mdl-12967146

ABSTRACT

Mycoplasmas identified as Mycoplasma canis were isolated from nine dogs with clinical signs of urogenital disease in Norway over a period of 20 months. Some of the dogs had been treated unsuccessfully with antibiotics, and three were euthanased as a result of severe persistent disease. Seven of the dogs had a urinary tract infection, one had chronic purulent epididymitis and one had chronic prostatitis. Overt haematuria was frequently observed among the dogs with cystitis. M canis was isolated in pure culture from seven of the dogs and in mixed culture from the other two. In three cases the mycoplasma was cultivated only from urinary sediment, and it was typically obtained in smaller numbers than would be considered indicative of a urinary tract infection. In contrast with most mycoplasmas, the M canis isolated from all the dogs grew on ordinary blood agar plates used for routine bacteriological cultivation. Specific mycoplasma media were not used and the presence of other Mycoplasma or Ureaplasma species cannot be excluded.


Subject(s)
Dog Diseases/microbiology , Female Urogenital Diseases/veterinary , Male Urogenital Diseases , Mycoplasma Infections/veterinary , Mycoplasma/isolation & purification , Animals , Dogs , Female , Female Urogenital Diseases/microbiology , Female Urogenital Diseases/physiopathology , Male , Mycoplasma/pathogenicity , Mycoplasma Infections/physiopathology , Norway
4.
Microb Drug Resist ; 7(3): 263-72, 2001.
Article in English | MEDLINE | ID: mdl-11759088

ABSTRACT

The presence of class 1 integrons was investigated in 38 sulfonamide-resistant strains of Aeromonas salmonicida subsp. salmonicida, atypical A. salmonicida and Escherichia coli conjugants with R plasmids originating from A. salmonicida. The strains originated from Finland, France, Japan, Norway, Scotland, Switzerland, and the United States. Additional resistance determinants in strains with class 1 integrons were also determined. Of 21 strains containing a class 1 integron, 19 had a single gene cassette, 1 strain had two cassettes, and 1 strain was found to lack an integrated gene cassette. In the integrons with single cassettes, aadA2 was present in eight strains, dfr16 in five strains, and aadA1 and dfrIIc in three strains each. In the integron with two cassettes, qacG and orfD were present. Tetracycline resistance was observed in 20 of the integron-positive strains, caused by the determinants Tet A and Tet E, in which Tet A frequently was associated with Tn1721. Class 1 integrons seem to be important in mediating antibiotic resistance also in the marine environment. The gene cassettes reported in this study are all described in bacteria associated with humans, and this demonstrates once more how the common gene pool is shared between organisms belonging to different environments.


Subject(s)
Aeromonas/drug effects , Aeromonas/genetics , Fish Diseases/microbiology , Fishes/microbiology , Gram-Negative Bacterial Infections/microbiology , Gram-Negative Bacterial Infections/veterinary , Integrins/genetics , Animals , Anti-Bacterial Agents/pharmacology , Blotting, Southern , Conjugation, Genetic , DNA Primers , Drug Resistance , Escherichia coli/drug effects , Gram-Negative Bacterial Infections/epidemiology , In Situ Hybridization , Microbial Sensitivity Tests , Phenotype , Plasmids/genetics , Reverse Transcriptase Polymerase Chain Reaction , Tetracycline/pharmacology
5.
Appl Environ Microbiol ; 66(12): 5533-5, 2000 Dec.
Article in English | MEDLINE | ID: mdl-11097945

ABSTRACT

Tn5393c containing strA-strB was identified as part of R plasmid pRAS2 from the fish pathogen Aeromonas salmonicida subsp. salmonicida. This is the first time an intact and active transposon in the Tn5393 family has been reported in an ecological niche other than an agricultural habitat.


Subject(s)
Aeromonas/genetics , DNA Transposable Elements/genetics , Fishes/microbiology , R Factors/genetics , Aeromonas/isolation & purification , Aeromonas/pathogenicity , Animals , Base Sequence , Chromosome Mapping , DNA, Bacterial/genetics , Drug Resistance, Microbial/genetics , Ecosystem , Genes, Bacterial , Molecular Sequence Data , Norway
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