High altitude arterialised capillary earlobe blood gas measurement using the Abbott i-STAT.

INTRODUCTION: Measurement of physiological parameters in extreme environments is essential to advancing knowledge, prophylaxis and treatment of altitude sickness. Point-of-care testing facilitates investigation in non-specialist and remote settings, as well as becoming increasingly popular at the bedside for real-time results in the clinical environment. Arterialised capillary earlobe blood gases are recommended as a valid alternative to arterial sampling in research. This study aimed to test the feasibility of obtaining and analysing daily earlobe samples at high altitude. METHODS: From 17 to 24 January 2016, 24 participants on a research expedition to Ecuador underwent daily earlobe blood gas measurements including pH, partial pressure of oxygen and partial pressure of carbon dioxide to 5043 m. Samples were analysed using an Abbott i-STAT blood gas analyser and G3+ cartridges. RESULTS: Daily measurements were successfully obtained and analysed at the point of care in 23/24 participants and were well tolerated with no adverse events. 12% (27/220) cartridges failed and required repeat sampling. CONCLUSIONS: Daily earlobe blood gas analysis using the Abbott i-STAT is feasible in a protected environment at high altitude. Participants and equipment should be kept warm before and during testing. Spare cartridges should be available. This methodology may be useful for both research and therapeutic measurements in remote, rural and wilderness medicine.

Temporal patterns of Deepwater Horizon impacts on the benthic infauna of the northern Gulf of Mexico continental slope.

The Deepwater Horizon oil spill occurred in spring and summer 2010 in the northern Gulf of Mexico. Research cruises in 2010 (approximately 2-3 months after the well had been capped), 2011, and 2014 were conducted to determine the initial and subsequent effects of the oil spill on deep-sea soft-bottom infauna. A total of 34 stations were sampled from two zones: 20 stations in the "impact" zone versus 14 stations in the "non-impact" zone. Chemical contaminants were significantly different between the two zones. Polycyclic aromatic hydrocarbons averaged 218 ppb in the impact zone compared to 14 ppb in the non-impact zone. Total petroleum hydrocarbons averaged 1166 ppm in the impact zone compared to 102 ppm in the non-impact zone. While there was no difference between zones for meiofauna and macrofauna abundance, community diversity was significantly lower in the impact zone. Meiofauna taxa richness over the three sampling periods averaged 8 taxa/sample in the impact zone, compared to 10 taxa/sample in the non-impact zone; and macrofauna richness averaged 25 taxa/sample in the impact zone compared to 30 taxa/sample in the non-impact zone. Oil originating from the Deepwater Horizon oil spill reached the seafloor and had a persistent negative impact on diversity of soft-bottom, deep-sea benthic communities. While there are signs of recovery for some benthic community variables, full recovery has not yet occurred four years after the spill.

Draft Genome Sequences for Canadian Isolates of Pectobacterium carotovorum subsp. brasiliense with Weak Virulence on Potato.

Pectobacterium carotovurum subsp. brasiliense causes soft rot and blackleg diseases on potato. Here, we report the draft genome sequences of three weakly virulent P. carotovurum subsp. brasiliense strains isolated in Canada. Analysis of these genome sequences will help to pinpoint differences in virulence among P. carotovurum subsp. brasiliense strains from tropical/subtropical and temperate regions, such as Canada and United States. A small number of key factors for adaptation to this bacterium's specific environmental niche were also evaluated.

On positive psychological outcomes: what helps groups with a history of conflict to forgive and reconcile with each other?

Three studies examined the roles of traditional and novel social psychological variables involved in intergroup forgiveness. Study 1 (N = 480) revealed that among the pro-Pinochet and the anti-Pinochet groups in Chile, forgiveness was predicted by ingroup identity (negatively), common ingroup identity (positively), empathy and trust (positively), and competitive victimhood (the subjective sense of having suffered more than the outgroup, negatively). Political ideology (Right vs. Left) moderated the relationship between empathy and forgiveness, trust and forgiveness, and between the latter and competitive victimhood. Study 2 (N = 309), set in the Northern Irish conflict between Protestants and Catholics, provided a replication and extension of Study 1. Finally, Study 3 (N = 155/108) examined the longitudinal relationship between forgiveness and reconciliation in Northern Ireland, revealing that forgiveness predicted reconciliation intentions. The reverse direction of this relationship was also marginally significant. Results are discussed in terms of their theoretical and practical implications.