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1.
Opt Express ; 31(3): 4423-4443, 2023 Jan 30.
Article in English | MEDLINE | ID: mdl-36785411

ABSTRACT

Efficient and refined three-dimensional (3D) reconstruction of industrial parts has become an urgent need in the field of advanced manufacturing, and it's a great challenge when facing in-motion and online inspection requirements of high dynamic range (HDR) surfaces that have large reflectivity variations. This paper proposes a method using RGB line-scan cameras to realize in-motion multiple-shot 3D shape measurements with RGB channel fusion to increase the measurement dynamic range. First, multi-channel one-dimensional background-normalized Fourier transform profilometry (MC-1DBNFTP) is proposed as an effective in-motion HDR method. Second, for HDR surfaces with strongly overexposed areas, we propose a solution that obtains 6 results of different dynamic ranges for fusion with only 5 projected patterns, which further extends the measurement dynamic range while ensuring the small projection period. Third, we develop a fusion method based on reliability evaluation, which is more reliable than the existing methods in fringe projection systems. In addition, colored textures can be mapped to the reconstructed surfaces. Experimental results prove that the proposed method realizes accurate and reliable in-motion 3D reconstruction of HDR surfaces.

2.
Opt Express ; 30(12): 21544-21567, 2022 Jun 06.
Article in English | MEDLINE | ID: mdl-36224872

ABSTRACT

The point cloud continuous measurements in some in-motion circumstances, such as quality inspection of products on assembly lines or rail traffic, have been requiring higher measurement speed, accuracy, and point cloud density. With the advantages of high acquisition rates and ultrahigh resolution, line-scan cameras have been developing gradually for dynamic measurements. However, because of non-coplanar installation and unidimensional images, the measurement based on line-scan cameras is affected by movement. In this article, a dynamic scanning point cloud measurement based on triple line-scan images is present. The point cloud optimization is based on bundle adjustment fused with motion information. The epipolar constraint of line-scan images in dynamic conditions is researched for matching. The effect of motion on matching error is analyzed. A triple line-scan cameras experimental setup validates the proposed method.

3.
BMC Plant Biol ; 22(1): 212, 2022 Apr 25.
Article in English | MEDLINE | ID: mdl-35468732

ABSTRACT

BACKGROUND: The wheat-rye 1BL.1RS translocations have played an important role in common wheat breeding programs. Subtelomeric tandem repeats have been often used to investigate polymorphisms of 1RS arms, but further research about their organizations on the 1RS chromosome is needed. RESULTS: 162 1RS arms from a wild rye species (Secale strictum) and six cultivated rye accessions (Secale cereale L.) (81 plants), 102 1BL.1RS and one 1AL.1RS translocations were investigated using oligo probes Oligo-TaiI, Oligo-pSc119.2-1, Oligo-pTa71A-2, Oligo-pSc200 and Oligo-pSc250, which were derived from tandem repeats TaiI, pSc119.2, pTa71, pSc200 and pSc250, respectively. The variations of 1RS arms were revealed by signal intensity of probes Oligo-pSc119.2-1, Oligo-pTa71A-2, Oligo-pSc200 and Oligo-pSc250. Proliferation of rDNA sequences on the 1RS chromosomes was observed. According to the presence of probe signals, 34, 127 and 144 of the 162 1RS arms contained TaiI, pSc200 and pSc250, respectively, and all of them contained pSc119.2 and pTa71. Most of the 1RS arms in rye contained three kinds of subtelomeric tandem repeats, the combination of pSc119.2, pSc200 and pSc250 was most common, and only eight of them contained TaiI, pSc119.2, pSc200 and pSc250. All of the 1RS arms in 1BL.1RS and 1AL.1RS translocations contained pSc119.2, pTa71, pSc200 and pSc250, but the presence of the TaiI family was not observed. CONCLUSION: New organizations of subtelomeric tandem repeats on 1RS were found, and they reflected new genetic variations of 1RS arms. These 1RS arms might contain abundant allelic diversity for agricultural traits. The narrow genetic base of 1RS arms in 1BL.1RS and 1AL.1RS translocations currently used in agriculture is seriously restricting their use in wheat breeding programs. This research has found new 1RS sources for the future restructuring of 1BL.1RS translocations. The allelic variations of these 1RS arms should be studied more intensely as they may enrich the genetic diversity of 1BL.1RS translocations.


Subject(s)
Chromosomes, Plant , Secale , Chromosomes, Plant/genetics , DNA, Ribosomal , Heterochromatin , In Situ Hybridization, Fluorescence , Plant Breeding , Secale/genetics , Tandem Repeat Sequences/genetics , Translocation, Genetic , Triticum/genetics
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