A Novel Intergenic Region (chr2: 30,316,870)-ALK Fusion in a Patient with Lung Adenocarcinoma Responding to Crizotinib Combined with Pemetrexed Treatment: A Case Report.

Background: Anaplastic lymphoma kinase (ALK) rearrangements have been reported as an important oncogenic driver in 5-7% non-small cell lung cancer (NSCLC) patients. Reports about the intergenic region (IGR) as an ALK fusion partner are rare. In this study, we report a novel IGR (chr2: 30,316,870)-ALK fusion in an advanced lung adenocarcinoma patient that responded effectively to crizotinib combined with pemetrexed. Case Presentation: A 68-year-old Chinese female was diagnosed with stage IV right lung adenocarcinoma (cT3N3M1c). The targeted next-generation sequencing (NGS) of 14 cancer-related genes identified an IGR (chr2: 30,316,870)-ALK fusion. Her lung lesions have been successfully converted from a partial response to a complete response after administrating crizotinib for 1 year combined with 6 cycles of chemotherapy with pemetrexed. So far, her progression-free-survival has reached 21 months. Conclusion: In this case, we firstly report a novel IGR (chr2: 30,316,870)-ALK fusion by using targeted NGS, and highlight the efficacy of crizotinib combined with pemetrexed to reduce unbearable gastrointestinal adverse reactions. It provides valuable clinical guidance for the treatment of similar cases in the future.

Efficient removal of tetracycline in water using modified eggplant straw biochar supported green nanoscale zerovalent iron: synthesis, removal performance, and mechanism.

A novel NaOH modified eggplant straw biochar supported green nanoscale zerovalent iron (P-nZVI/ESBC) composite was synthesized and its removal performance and reaction mechanism for tetracycline (TC) in water were investigated. Multiple characterizations showed that the prepared P-nZVI/ESBC composite contained oxygen-containing functional groups (hydroxyl, carbonyl, and carboxyl groups) and Fe species (nZVI and its oxides). The dosage of composite, temperature, and solution pH significantly affected the removal capacity of the P-nZVI/ESBC composite for TC. The Avrami fraction-order kinetic model and Sips adsorption isotherm model can fit well the removal process of TC by the P-nZVI/ESBC composite, indicating that the adsorption behavior of TC involved multiple adsorption mechanisms and chemical adsorption might occur. The maximum adsorption capacity of the P-nZVI/ESBC composite for TC was 304.62 mg g-1. The adsorption and reductive degradation were the dominant mechanisms of TC removal by the P-nZVI/ESBC composite. This work offers abundant information on the application of eggplant straw to manufacture biochar-based composites for the efficient removal of antibiotic contaminants from aquatic environments.

A fluorescent sensor based on sulfur nanodots encapsulated into zeolitic imidazolate framework-8 for ultrasensitive detection of tartrazine.

A new composite material (SDs@ZIF-8) was synthesized by integrating sulfur nanodots (SDs) into metal-organic frameworks (ZIF-8) through a facile one-step self-assembly strategy. The obtained SDs@ZIF-8 has not only the high adsorption performance of ZIF-8 but also the superior fluorescence characteristics of SDs. The composite featured good dispersibility, stable structure as well as excellent fluorescence in water solution, and can be used as an ideal fluorescent sensor for tartrazine detection. Due to the high specific surface area and adsorption performance of ZIF-8, the prepared composite material can significantly enrich tartrazine, further enhancing the sensitivity of analysis. The fluorescence of SDs @ZIF-8 composite can be effectively quenched by tartrazine through the inner filter effect. The sensing technique exhibited exceptional sensitivity, as evidenced by its impressive detection limit of 6.5 nM across a broad linear range spanning from 0.02 to 90 µM. In addition to its high sensitivity, the technique displayed rapid response times and excellent selectivity. Moreover, the fluorescent sensing technology we developed has been employed successfully for the detection of tartrazine in real samples, which is expected to promote the development of the food safety industry.

Convenient green synthesis of Cu/Fe nanoparticles using pomegranate peel extracts and their performance for tetrabromobisphenol A removal.

In this work, pomegranate peel extracts were used as the green reducing agent to synthesize Cu/Fe nanoparticles (P-Cu/Fe nanoparticles) and removed tetrabromobisphenol A (TBBPA) in aqueous solution. P-Cu/Fe nanoparticles were amorphous and irregularly spherical. The surfaces of nanoparticles contained Fe0, Fe3+ oxides (hydroxides), and Cu0. The bioactive molecules from pomegranate peel were extremely important for the synthesis of nanoparticles. P-Cu/Fe nanoparticles had excellent removal performance for TBBPA, and 98.6% of TBBPA (5 mg L-1) was removed within 60 min. The removal reaction of TBBPA by P-Cu/Fe nanoparticles was well-fitted with the pseudo-first-order kinetic model. The Cu loading was critical for TBBPA removal with an optimum value of 1.0 wt%. A weakly acidic condition (pH 5) was more favorable for the removal of TBBPA. The removal efficiency of TBBPA increased with the rise of temperature and decreased with increasing initial TBBPA concentration. The activation energy (Ea) was 54.09 kJ mol-1, indicating that the removal of TBBPA by P-Cu/Fe nanoparticles was mainly surface-controlled. Reductive degradation was the main mechanism of TBBPA removal by P-Cu/Fe nanoparticles. In conclusion, green synthesized P-Cu/Fe nanoparticles using pomegranate peel waste show great potential for the remediation of TBBPA in aqueous solution.

EEF1A2 accelerates the protein translation of chemokine in rat myocardial cells induced by ischemia-reperfusion.

How to reduce the damage caused by myocardial ischemia-reperfusion (IR) in a timely manner to save patients' lives is still a great clinical challenge. Although dexmedetomidine (DEX) has been reported to protect the myocardium, the regulatory mechanism of gene translation responding to IR injury and DEX protection is poorly understood. In this study, IR rat model with DEX and the antagonist yohimbine (YOH) pretreatment were established, and RNA sequencing was carried out to seek the important regulators in differential expressed genes. A series of cytokines and chemokine as well as eukaryotic translation elongation factor 1 alpha 2 (EEF1A2) were induced by IR compared to control and compromised by DEX pretreatment compared to IR, then reversed by YOH. Immunoprecipitation was conducted to identify that peroxiredoxin 1 (PRDX1) interacted with EEF1A2 and contributed to the recruitment of EEF1A2 on mRNA molecules of cytokines and chemokine. Knockdown of PRDX1 could weaken the enhancive effect of EEF1A2 for gene translation of IL6, CXCL2 and CXCL11 under the IR condition, and indeed reduce cell apoptosis of cardiomyocytes. We also determined that the RNA motif "USCAGDCU" at 5' UTR could be particularly recognized by PRDX1. Destruction of this motif at the 5' UTR of IL6, CXCL2 and CXCL11 by CRISPR-CAS9 could result in the loss occupancies of EEF1A2 and PRDX1 on the mRNA of these three genes. Our observations showed the importance of PRDX1 in the reasonable control of cytokine and chemokine expression to prevent excessive inflammatory response to cell damage.

The relationship between perceived social support with anxiety, depression, and insomnia among Chinese college students during the COVID-19 pandemic: The mediating role of self-control.

Object: We aimed to investigate the associations between perceived social support and anxiety, depression, and sleep disturbance via self-control among Chinese college students during the COVID-19 pandemic. Materials and methods: The Perceived Social Support Scale, Self-control Scale, Self-rating Anxiety Scale, Self-rating Depression Scale, and Insomnia Severity Index Scale were used to survey 1,997 college students during the COVID-19 pandemic, who submitted valid questionnaires (M age = 19.93, SD age = 1.47, Range = 18-24 years, 62% female). Results: The perceived social support and self-control were significantly positively correlated, and they were significantly and negatively associated with anxiety, depression, and insomnia. Further analysis found that self-control partially mediated the relationships between perceived social support with anxiety, depression, and insomnia. Conclusion: During the COVID-19 pandemic, Chinese college students' self-control played a partial mediating effect in the relationships between perceived social support and anxiety, depression, and insomnia. This study provides new insights and inspiration for improving college students' mental health in the context of the pandemic.

Cucumber (Cucumis sativus L.) Leaf Extract as a Green Corrosion Inhibitor for Carbon Steel in Acidic Solution: Electrochemical, Functional and Molecular Analysis.

An extract of cucumber leaves (ECSL) was prepared as a green corrosion inhibitor for carbon steel. Its carbon steel corrosion inhibition performance against 0.5 mol L-1 H2SO4 was investigated using electrochemical methods and scanning electron microscopy (SEM). Its composition was analyzed by gas chromatography and mass spectroscopy (GC-MS). Quantum chemical calculations and molecular dynamics simulations (MDS) were conducted to elucidate the adsorption mechanism of the inhibitor molecules on the carbon steel surface. The results indicated that the inhibition efficiency increases with its increasing concentration. The extract acted as a mixed type corrosion inhibitor, and its inhibition properties were ascribed to the geometric coverage effect induced by its adsorption on the metal surface in accordance with Langmuir's law. The active components in the extract were identified as mainly organic compounds with functional groups such as aromatic moieties and heteroatoms. The inhibition activities of ECSL are delivered through the ability of the active components to adsorb on the metal surface through their functional groups to form a protective layer which hinders the contact of aggressive substances with carbon steel and thus suppresses its corrosion. This research provides an important reference for the design of green corrosion inhibitors based on plant waste materials.

Comparative Study on Recognition Models of Black-Odorous Water in Hangzhou Based on GF-2 Satellite Data.

To improve the ability of remote sensing technology in recognizing black-odorous water bodies in Hangzhou, this study analyzed the typical spectral characteristics of black-odorous water in Hangzhou based on measured spectral data and water quality parameters, including the transparency, dissolved oxygen, oxidation reduction potential, and ammonia nitrogen. The single-band threshold method, the normalized difference black-odorous water index (NDBWI) model, the black-odorous water index (BOI) model, and the color purity on a Commission Internationale de L'Eclairage (CIE) model were compared to analyze the spatial and temporal distribution characteristics of the black-odorous water in Hangzhou. The results showed that: (1) The remote sensing reflectance of black-odorous water was lower than that of ordinary water, the spectral curve was gentle, and the wave peak shifted toward the near-infrared direction in the wavelength range of 650-850 nm; (2) Among the aforementioned models, the normalized and improved normalized black-odorous water index methods had a higher accuracy, reaching 87.5%, and the threshold values for black-odorous water identification were 0.14 and 0.1, respectively; (3) From 2015 to 2018, the quantity of black-odorous water in the main urban area of Hangzhou showed a decreasing trend, and black-odorous water was mainly distributed in the Gongshu District and tended to appear in narrow rivers, densely populated areas, and factory construction sites. This study is expected to be of great practical value for the rapid tracking and monitoring of urban black-odorous water by using remote sensing technology for future work.

The relationship between schizotypal personality features and mind wandering among college students during COVID-19 pandemic: A moderator of depression.

Introduction: Although the impact of the COVID-19 pandemic on people's mental health has been well documented in many studies, the schizotypal personality features in the general population have not received sufficient attention. Methods: Study 1 is a longitudinal study tracking changes in schizotypal personality features among college students during the COVID-19 pandemic. A total of 153 Chinese college students were assessed using the Schizotypal Personality Questionnaire. Study 2 explored the relationship between schizotypal personality features, mind wandering, and depression. A total of 557 college students completed the Schizotypal Personality Questionnaire, the Beck Depression Inventory, and the Mind-Wandering Questionnaire during the COVID-19 pandemic. Results: Study 1 results showed that the scores from later stages in the pandemic were significantly higher than those from the initial stages on each dimension of schizotypal personality, which means that the schizotypal personality features became more obvious during the COVID-19 pandemic. Study 2 results showed that there was a positive correlation between schizotypal personality features, depression, and mind wandering. Discussion: Depression played a moderating role in the relationship between schizotypal personality features and mind wandering. The schizotypal personality features of college students increase during COVID-19; it has a positive relationship with mind wandering; depression moderates the relationship. We discussed these findings and provided some suggestions about future research.

A new method for cartap detection with high sensitivity and selectivity based on the inner filter effect between GSH-Cu NCs and Au NPs.

Herein, a novel and sensitive fluorescence method for cartap determination is established on the basis of the inner filter effect (IFE) of gold nanoparticles (Au NPs) on the fluorescence of glutathione protected Cu NCs (GSH-Cu NCs). In the presence of Au NPs, the fluorescence of GSH-Cu NCs was strongly quenched by the IFE because the absorption spectra of Au NPs overlap well with the emission spectra of GSH-Cu NCs. Upon addition of cartap, cartap could induce the aggregation of Au NPs whose absorption spectrum does not overlap with the emission spectrum of GSH-Cu NCs. Then, with the increase in cartap concentration, the IFE-decreased fluorescence was gradually recovered, realizing the fluorescence sensing of cartap. Under optimal conditions, the proposed method has a good linear relationship with cartap concentration in the range of 7-100 nM, and the detection limit is 3.34 nM. In addition, satisfactory results were obtained for cartap analysis using tap water and cabbage as real samples, which demonstrated that the method as-developed would have great practical application prospects.

A fluorescence sensing method for brilliant blue with gold nanoclusters based on the inner filter effect.

Due to the inner filter effect (IFE) between Brilliant Blue (BB) and gold nanoclusters (AuNCs), a simple and rapid approach for BB detection was developed. Since the absorption spectrum of BB has a good overlap with the emission spectrum of the AuNCs, the fluorescence of the AuNC solution was quenched after adding BB. Under optimal conditions, a good linear relationship for detection of BB was obtained from 0.05 to 7.5 µM, and the detection limit was 0.0167 µM. More importantly, the assay was successfully applied to assess BB in food samples such as hard candy, chocolate and dried blueberries, showing its potential in food quality control in the future.

Ratiometric fluorescent sensor for visual determination of copper ions and alkaline phosphatase based on carbon quantum dots and gold nanoclusters.

In this work, a novel ratiometric fluorescent sensor, based on carbon dots (CDs) and gold nanoclusters (AuNCs), is developed for highly sensitive and selective visual colorimetric detection of Cu2+ and alkaline phosphatase (ALP). The ratiometric fluorescent sensor was synthesized by covalently linking 11-mercaptoundecanoic acid (11-MUA)-stabilized AuNCs to the surface of amino-functionalized CD/SiO2 nanoparticles. The red fluorescence of the AuNCs can be quenched by Cu2+ owing to coordination between Cu2+ and 11-MUA; however, the blue emission of the CDs was insensitive to Cu2+ owing to the protective silica shell. The quenching of the AuNCs' fluorescence returned when PPi was added because of the higher affinity between Cu2+ and PPi than that between Cu2+ and 11-MUA. In the presence of ALP, PPi was catalytically hydrolyzed into phosphate (Pi), which showed a much weaker affinity for Cu2+. Thus, Cu2+ ions were released, and the fluorescence of the AuNCs was quenched once more. Based on this principle, Cu2+ and ALP could be simultaneously detected. The developed ratiometric fluorescent sensor could detect Cu2+ over a range from 0.025 to 4 µM with a detection limit of 0.013 µM and ALP over a range from 0.12 to 15 U/L with a detection limit of 0.05 U/L. The present method was successfully applied for the detection of Cu2+ and ALP in real water samples and in human serum samples, respectively. This ratiometric fluorescent approach may provide a highly sensitive and accurate platform for visual Cu2+ and ALP sensing in environmental monitoring and medical diagnosis.

Pretreatment albumin/fibrinogen ratio as a promising predictor for the survival of advanced non small-cell lung cancer patients undergoing first-line platinum-based chemotherapy.

BACKGROUND: This study aimed to identify potential predictive factors for the survival of advanced non small-cell lung cancer (NSCLC) patients undergoing first-line platinum-based chemotherapy. METHODS: A total of 270 advanced NSCLC patients who underwent first-line platinum-based chemotherapy from June, 2011 to June, 2015 were enrolled. A receiver operating characteristic (ROC) curve analysis was used to evaluate the predictive value of the albumin-to-fibrinogen ratio (AFR) for overall survival (OS). The predictive factors for survival were evaluated by univariate and multivariate analyses via the Cox proportional hazards regression model. The OS and progression free survival (PFS) results were determined via the Kaplan-Meier method using the log-rank analysis. RESULTS: Based on the results of the ROC curve analysis, 8.02 was accepted as the cut-off AFR value for OS. The metastasis stage (M0 vs M1a/b, HR: 1.73, 95% CI: 1.15-2.59, P = 0.020) and AFR (≤8.02 vs > 8.02, HR: 1.80, 95% CI: 1.09-2.78, P = 0.025) were two independent risk factors for PFS by multivariate Cox regression analysis. The AFR (≤8.02 vs > 8.02, HR: 1.79, 95% CI: 1.11-2.59, P = 0.029) was a significant predictive factor for OS in advanced NSCLC patients. The PFS (P = 0.008) and OS (P = 0.003) in the high AFR group were significantly improved compared with those in the low AFR group via the Kaplan-Meier method using the log-rank analysis. CONCLUSIONS: The AFR could be a potential effective predictive factor for the survival in advanced NSCLC patients undergoing first-line platinum-based chemotherapy.

Low-Temperature Superplasticity and Deformation Mechanism of Ti-6Al-4V Alloy.

The low-temperature superplastic tensile behavior and the deformation mechanisms of Ti-6Al-4V alloy are investigated in this paper. Through the experiments carried out, elongation to failure (δ) is calculated and a set of values are derived that subsequently includes the strain rate sensitivity exponent (m), deformation activation energy (Q) at low-temperature superplastic deformation, and the variation of δ, m and Q at different strain rates and temperatures. Microstructures are observed before and after superplastic deformation. The deformation mechanism maps incorporating the density of dislocations inside grains at temperatures of 973 and 1123 K are drawn respectively. By applying the elevated temperature deformation mechanism maps based on Burgers vector compensated grain size and modulus compensated stress, the dislocation quantities and low-temperature superplastic deformation mechanisms of Ti-6Al-4V alloy at different temperatures within appropriate processing regime are elucidated.

Inhibition of MicroRNA-23 Contributes to the Isoflurane-Mediated Cardioprotection Against Oxidative Stress.

Isoflurane is one of the most frequently used volatile anesthetics in clinical practice for inhalational anesthesia. It is widely studied that isoflurane mediates cardioprotection during multiple pathological processes. However, the precise mechanisms have not been fully elucidated. Neonatal cardiomyocytes were isolated and cultured, followed by treatments with isoflurane at 0, 50, 100 or 200 µM. Rat cardiomyoblast cell line, H9c2, was treated with H2O2. Expression of miR-23 was measured by qRT-PCR. The cell survival rate of H9c2 in response to H2O2 treatments was evaluated by MTT assay. The ROS and GSH/GSSG levels were measured using Superoxide Detection Kit and GSH/GSSG Ratio Detection Assay Kit. In this study, we report an isoflurane-miR-23-antioxidant axis in cardiomyocyte. We observed that miR-23 was suppressed by isoflurane treatments at 50, 100 or 200 µM. Moreover, cardiomyocyte with isoflurane exposure was insensitive to H2O2 treatment in vitro. Inhibition of miR-23 protected cardiomyocyte against oxidative stress induced by H2O2 treatments at 30, 60, 90 or 120 µM. In addition, overexpression of miR-23 induced ROS generation over twofolds and rendered cardiomyocyte sensitive to H2O2 treatments. We demonstrate that miR-23 inhibited intracellular GSH, an antioxidant against oxidative stress. Our results reveal that with isoflurane exposure, overexpression of miR-23 rendered cardiomyocyte sensitive to H2O2 treatments at 20, 30, 40, 50 µM. Pretreatments with GSH in miR-23 overexpressing cells rescued the cell death under oxidative stress. In summary, our results illustrate that the isoflurane-mediated protection of cardiomyocytes under oxidative stress is through inhibition of miR-23. This study provides an aspect for the miRNAs-modulated cardiomyocyte sensitivity to oxidative stress, contributing to the development of therapeutic agents.

Chronotropic Incompetence and its Relation to Exercise Intolerance in Chronic Obstructive Pulmonary Disease.

BACKGROUND: To study the relationship between chronotropic incompetence (CI) and disease severity and to assess the effect of CI on exercise capacity in patients with chronic obstructive pulmonary disease (COPD). MATERIALS AND METHODS: Arterial blood gas analysis, pulmonary function test and cardiopulmonary exercise testing were conducted in 60 patients with stable COPD and 45 healthy volunteers. CI was defined using the chronotropic response index (CRI = (peak heart rate-resting heart rate) / (220-age-resting heart rate). Based on CRI, patients with COPD were divided into the normal chronotropic group (n = 23) and CI group (n = 37). RESULTS: CI was present in 61.7% of the patients with COPD. Exercise capacity (peak oxygen uptake as percentage of predicted value, peak VO2%pred), peak heart rate and CRI were significantly lower in patients with COPD than in controls. However, resting heart rate was significantly higher than in controls. FEV1%pred and exercise capacity were significantly decreased in the CI group when compared with those in the normotropic group. There was significant association between CRI with FEV1%pred and peak VO2%pred. Multivariate regression analysis showed that CRI and FEV1%pred were independent predictors of exercise capacity in patients with COPD. A cutoff of 0.74 for the CRI showed a specificity of 94.1% in predicting patients with a peak VO2%pred < 60%. CONCLUSIONS: CRI was associated with disease severity in patients with COPD. CI may be an important parameter to reflect exercise capacity in patients with COPD.

A Turn-On Fluorescent Sensor for Selective and Sensitive Detection of Alkaline Phosphatase Activity with Gold Nanoclusters Based on Inner Filter Effect.

In this work, a novel approach for simple and sensitive determination of alkaline phosphatase (ALP) is developed on the basis of an inner filter effect of p-nitrophenylphosphate (PNPP) on the fluorescence of gold nanoclusters (AuNCs). AuNCs with a high quantum yield of 12% were synthesized by one-pot strategy and were directly applied as fluorescent substance. When AuNCs were mixed with PNPP, the fluorescence of the AuNCs was remarkably quenched or was decreased via the inner filter effect since the absorption spectrum of PNPP overlaps well with the excitation spectrum of the AuNCs. While in the presence of ALP, PNPP was catalytically hydrolyzed into p-nitrophenol, which has different absorption characteristics from those of PNPP, resulting in the recovery of the AuNCs fluorescence. Thus, a novel "turn-on" fluorescent sensor for detecting ALP was established with a detection limit as low as 0.002 U/L (signal-to-noise ratio of 3). The turn-on fluorescent sensor exhibits many merits such as high sensitivity, excellent selectivity, and high signal output because of the low background signals. In addition, the developed sensing method was successfully applied to investigate ALP inhibitors and ALP determination in serum samples. A good linear relationship was obtained in the range from 0.02 to 50 U/L, and satisfactory recoveries at four spiking levels of ALP ranged from 95% to 106% with precision below 5%. The very simple sensing approach proposed here should promote the development of fluorescence turn-on chemosensors for chemo/biodetection.

Ratiometric fluorescence and mesoporous structured imprinting nanoparticles for rapid and sensitive detection 2,4,6-trinitrophenol.

The present study reports the fabrication of mesoporous-structured ratiometric molecularly imprinted sensors using a combined surface-imprinted and ratiometric fluorescence method. The sensors were subsequently examined in the selective and sensitive determination of 2,4,6-trinitrophenol (TNP). In the preparation of the ratiometric system, the reference dye CdTe quantum dots were embedded in silica core particles via the Stöber method; the functional target sensitive dye AAMBT&SiO2, which was obtained via polymerization of 2-acrylamide-6-methoxybenzothiazole (AAMBT) with allyltriethoxysilane, was embedded in the mesoporous silica shell. In the surface imprinting process, cetyltrimethylammonium bromide was employed to create mesoporous-structured silica to promote quenching of AAMBT by TNP via resonance energy transfer, thereby enhancing the sensitivity of the sensor. Under optimum conditions, the ratiometric fluorescence molecularly imprinted polymer sensors achieved a detection limit of 43nM within 3min. The practical application of the developed sensor in real water samples was successfully demonstrated through analysis of TNP in water samples, achieving satisfactory recoveries of 92-104%. Thus, a convenient and practical method for preparing highly selective and sensitive ratiometric fluorescence sensors is presented herein, providing a prospective method for rapid trace pollutants analysis in complex water samples.

Sensitive arginine sensing based on inner filter effect of Au nanoparticles on the fluorescence of CdTe quantum dots.

Arginine plays an important role in many biological functions, whose detection is very significant. Herein, a sensitive, simple and cost-effective fluorescent method for the detection of arginine has been developed based on the inner filter effect (IFE) of citrate-stabilized gold nanoparticles (AuNPs) on the fluorescence of thioglycolic acid-capped CdTe quantum dots (QDs). When citrate-stabilized AuNPs were mixed with thioglycolic acid-capped CdTe QDs, the fluorescence of CdTe QDs was significantly quenched by AuNPs via the IFE. With the presence of arginine, arginine could induce the aggregation and corresponding absorption spectra change of AuNPs, which then IFE-decreased fluorescence could gradually recover with increasing amounts of arginine, achieving fluorescence "turn on" sensing for arginine. The detection mechanism is clearly illustrated and various experimental conditions were also optimized. Under the optimum conditions, a decent linear relationship was obtained in the range from 16 to 121µgL-1 and the limit of detection was 5.6µgL-1. And satisfactory results were achieved in arginine analysis using arginine injection, compound amino acid injection, even blood plasma as samples. Therefore, the present assay showed various merits, such as simplicity, low cost, high sensitivity and selectivity, making it promising for sensing arginine in biological samples.