Long non-coding RNA CCAT2 promotes gastric cancer proliferation and invasion by regulating the E-cadherin and LATS2.

Dysregulation of long non-coding RNAs (lncRNAs) play important roles in tumor development and progression. The long non-coding RNA CCAT2 has been identified to be up-regulated in gastric cancer (GC). However, the detailed molecular mechanism of CCAT2 involved in GC progression is still unknown. The aim of this study was to explore the expression and role of CCAT2 in GC progression. In the study, the expression levels of CCAT2 were significantly up-regulated in 108 cases GC tissues compared with adjacent non-tumor tissues by qRT-PCR analysis. Higher CCAT2 expression was correlated with tumor size, lymph node metastasis and Tumor Node Metastasis (TNM) stage in GC patients. Multivariate analysis showed that lymph node metastasis, TNM stage and the expression of CCAT2 were independent prognostic indicator for disease-free survival (DFS) and the over survival time (OS) for GC patients. Further function analysis demonstrated that knockdown of CCAT2 inhibited the cell migration and invasion, whereas, the overexpression of CCAT2 showed the opposite results in GC cells. Our results also demonstrated that CCAT2 promoted the GC cells epithelial-mesenchymal transition (EMT) by downregulated the E-cadherin expression and upregulated the ZEB2, Vimentin and N-cadherin expression. Moreover, RNA immunoprecipitation (RIP) and Chromatin immunoprecipitation (ChIP) revealed that CCAT2 interacted with EZH2 and regulated the E-cadherin and LATS2 expression. Thus, our results demonstrated that CCAT2 functioned as an oncogene in GC and was involved in gastric cancer progression. Targeting CCAT2 might be a potential therapeutic target for GC.

[Effects of Na-FA on gastrointestinal movement and gastric ulcer in mice].

OBJECTIVE: To research the effects of Na-FA on gastrointestinal movement and gastriculcer in mice. METHODS: Using charcoal powder as an indicator, the effects of FA-Na on bowel movement of mice was evaluated by determining the propulsive percent-age of charcoal powder in normal mice, inhibition of small intestinal propulsion model induced by Compound Diphenoxylate and spleen asthenia and diarrhea model caused by Rheum officinale Baill; The acute gastric ulcer model was induced by intragastric alcohol given orally, the protective effect of FA-Na given ahead on gastric injury was evaluated, the ulcer index and the inhibition ratio of ulcer was calculated. RESULTS: FA-Na (intragastric administration) had inhibit effect on normal mice and diarrhea model caused by Rheum officinale, significantly synergetic effect on gastrointestinal asynersis induced by Compound Diphenoxylate, and protective effect on mice gastric mucosal injury cause by dehydrated alcohol. Compared with the positive control drug Ranitidine, the ulcer index and ulcer inhibition ratio large doses of FA-Na had no significant difference. CONCLUSION: The acute toxicity of FA-Na is very low, it has the effects of antidiarrheal and anti-gastric ulcer.

[Determination of two main components in bark of Paeonia suffruticosa by HPLC].

OBJECTIVE: To establish a HPLC method for determination two constituents in bark of Paeonia Suffuticosa. METHOD: The reversed phase HPLC system consisting of an Alltima ODS column (4.6 mm x 150 mm, 5 microm) and a mixture of water-THF-methanol-HAc (60:20:20:0.05) as the mobile phase was used. The flow rate was 0.8 mL x min(-1) and UV detection was set at 274 nm. RESULT: The assay displayed good linearity over the concentration ranges of 0.06-1.0 microg (r = 0.999 9, gallic acid) and 0.16-2.58 microg (r = 0.999 9, paeonol) respectively. The average recoveries (n = 9) of gallic acid and paeonol were 98.6% (RSD = 3.0%), 98.2% (RSD = 2.5%), respectively. The samples were extracted with methanol for 24 h bu maceration. CONCLUSION: The method is simple, accurate and can be used for the quality study of bark of P. suffruticosa.