Multi-omics profiling reveal cells with novel oncogenic cluster, TRAP1low/CAMSAP3low, emerge more aggressive behavior and poor-prognosis in early-stage endometrial cancer.

The clinical heterogeneity of early-stage endometrial cancer (EC) is worthy of further study to identify high-quality prognostic markers and their potential role in aggressive tumor behavior. Mutation of TP53 was considered as an important primary triage in modified molecular typing for EC, it still cannot precisely predict the prognosis of EC. After proteomic analysis of cancer and para-cancerous tissues from 24 early-stage endometrioid EC patients with different survival outcomes, 13 differentially expressed proteins were screen out while 2 proteins enriched in p53 signaling pathway were further identified by single-cell transcriptome (scRNA-seq). Interestingly, tumor necrosis factor type-1 receptor-associated protein (TRAP1) and calmodulin-regulated spectrin-associated protein family member 3 (CAMSAP3) were found to be significantly downregulated in the specific cell cluster. Expectedly, the signature genes of TRAP1low/CAMSAP3low cluster included classical oncogenes. Moreover, close cellular interactions were observed between myeloid cells and the TRAP1low/CAMSAP3low cluster after systematically elucidating their relationship with tumor microenvironment (TME). The expression of TRAP1 and CAMSAP3 was verified by immunohistochemistry. Thus, a novel prediction model combining TRAP1, CAMSAP3 and TP53 was construct by multi-omics. Compared with the area under the curve, it demonstrated a significantly improvemrnt in the diagnostic efficacy in EC patients from TCGA bank. In conclusion, this work improved the current knowledge regarding the prognosis of early-stage EC through proteomics and scRNA-seq. These findings may lead to improvements in precise risk stratification of early-stage EC patients.

Polystyrene nanoplastics distinctly impact cadmium uptake and toxicity in Arabidopsis thaliana.

The ubiquitous presence of micro- and nanoplastics (MNPs) in soil has raised concerns regarding their potential effects on terrestrial plants. The coexistence and interactions between MNPs and heavy metals altering their phytotoxicity deserves further investigation. In this study, we explored the impacts of various concentrations of polystyrene nanoplastics (PS-NPs) and cadmium (Cd) alone or in combination on the growth and development of Arabidopsis thaliana. Additionally, we examined the effects of combined stress on the uptake and translocation of Cd within Arabidopsis thaliana. Our findings revealed several key insights: PS-NPs exhibited the capability to internalize in the maturation zone of Arabidopsis roots; the presence of Cd changed the particle size and zeta potential of PS-NPs; the presence of PS-NPs heightened Cd accumulation in the underground parts of Arabidopsis seedlings, leading to a stronger oxidative stress response in these regions; the composite stress exerted a more pronounced effect on the growth and development of Arabidopsis compared to individual stresses. Interestingly, while higher PS-NPs concentrations hindered Cd migration from roots to leaves, they also acted as carriers for Cd uptake in Arabidopsis roots. These findings shed light on the combined impacts of MNPs and heavy metals on plant physiology, offering theoretical insights to guide risk assessment strategies for MNPs and heavy metals in terrestrial ecosystems.

Microalgae Film-Derived Water Evaporation-Induced Electricity Generator with Negative Carbon Emission.

Water evaporation-induced electricity generators (WEGs) are regarded as one of the most promising solutions for addressing the increasingly severe environmental pollution and energy crisis. Owing to the potential carbon emission in the preparation process of WEGs, whether WEG represents a clean electricity generation technology is open to question. Here, a brand-new strategy is proposed for manufacturing negative carbon emission WEG (CWEG). In this strategy, the microalgae film is used as the electricity generation interface of WEG, which achieves a stable open-circuit voltage (Voc) of 0.25 V and a short-circuit current (Isc) of 3.3 µA. Since microalgae can capture carbon dioxide during its growing process, CWEG holds great promise to generate electricity without carbon emissions in the full life cycle compared with other WEGs. To the best of the author's knowledge, this is the first work using microalgae films to fabricate WEG. Therefore, it is believed that this work not only provides a new direction for designing high-efficiency and eco-friendly WEG but also offers an innovative approach to the resource utilization of microalgae.

Microfluidic sensors for the detection of emerging contaminants in water: A review.

In recent years, numerous emerging contaminants have been identified in surface water, groundwater, and drinking water. Developing novel sensing methods for detecting diverse emerging pollutants in water is urgently needed, as even at low concentrations, these pollutants can pose a serious threat to human health and environmental safety. Traditional testing methods are based on laboratory equipment, which is highly sensitive but complex to operate, costly, and not suitable for on-site monitoring. Microfluidic sensors offer several benefits, including rapid evaluation, minimal sample usage, accurate liquid manipulation, compact size, automation, and in-situ detection capabilities. They provide promising and efficient analytical tools for high-performance sensing platforms in monitoring emerging contaminants in water. In this paper, recent research advances in microfluidic sensors for the detection of emerging contaminants in water are reviewed. Initially, a concise overview is provided about the various substrate materials, corresponding microfabrication techniques, different driving forces, and commonly used detection techniques for microfluidic devices. Subsequently, a comprehensive analysis is conducted on microfluidic detection methods for endocrine-disrupting chemicals, pharmaceuticals and personal care products, microplastics, and perfluorinated compounds. Finally, the prospects and future challenges of microfluidic sensors in this field are discussed.

Immobilisation remediation of arsenic-contaminated soils with promising CaAl-layered double hydroxide and bioavailability, bioaccessibility, and speciation-based health risk assessment.

Arsenic (As)-contaminated soil poses great health risk to human mostly through inadvertent oral exposure. We investigated CaAl-layered double hydroxide (CaAl-LDH), a promising immobilising agent, for the remediation of As-contaminated Chinese soils. The effects on specific soil properties and As fractionation were analyzed, and changes in the health risk of soil As were accurately assessed by means of advanced in vivo mice model and in vitro PBET-SHIME model. Results showed that the application of CaAl-LDH significantly increased soil pH and concentration of Fe and Al oxides, and effectively converted active As fractions into the most stable residual fraction, guaranteeing long-term remediation stability. Based on in vivo test, As relative bioavailability was significantly reduced by 37.75%. Based on in vitro test, As bioaccessibility in small intestinal and colon phases was significantly reduced by 25.65% and 28.57%, respectively. Furthermore, As metabolism (reduction and methylation) by the gut microbiota inhabiting colon was clearly observed. After immobilisation with CaAl-LDH, the concentration of bioaccessible As(Ⅴ) in the colon fluid was significantly reduced by 61.91%, and organic As (least toxic MMA(V) and DMA(V)) became the main species, which further reduced the health risk of soil As. In summary, CaAl-LDH proved to be a feasible option for immobilisation remediation of As-contaminated soils, and considerable progress was made in relevant health risk assessment.

The effect of life events on NSSI: the chain mediating effect of sleep disturbances and PLEs among Chinese college students.

This study aimed to explore the relationship between life events and non-suicidal self-injury (NSSI) in college students, as well as the mediating effect of sleep disturbances and psychotic-like experiences (PLEs). After excluding invalid questionnaires, 5,754 were retained, and the valid efficiency was 75.94%. The subjects were aged 16 to 29 years (M = 19.166; SD = 1.392), with 1,969 males (34.22%) and 3,785 females (65.78%). Life events, sleep disturbances, PLEs, and NSSI were assessed using standard scales. Data were analyzed by Pearson Correlation Analysis and bias-correction percentile Bootstrap method. The results show that (1) life events were significant positive predictors of NSSI, sleep disturbances, and PLEs; (2) sleep disturbances, PLEs, and the chain mediation between the two, were mediators between life events and NSSI. Life events are thus shown to be an important external factor influencing NSSI in university students, and this process is mediated through sleep disturbances, PLEs, and the chain between the two. Interventions for NSSI can therefore be made by improving college students' sleep quality and reducing PLEs.

Influences of coexisting aged polystyrene microplastics on the ecological and health risks of cadmium in soils: A leachability and oral bioaccessibility based study.

Whether coexisting microplastics (MPs) affect the ecological and health risks of cadmium (Cd) in soils is a cutting-edge scientific issue. In this study, four typical Chinese soils were prepared as artificially Cd-contaminated soils with/without aged polystyrene (PS). TCLP and in vitro PBET model were used to determine the leachability (ecological risk) and oral bioaccessibility (human health risk) of soil Cd. The mechanisms by which MPs influence soil Cd were discussed from direct and indirect perspectives. Results showed that there was no significant difference in the leachability of soil Cd with/without aged PS. Additionally, aged PS led to a significant decrease in the bioaccessibility of soil Cd in gastric phase, but not in small intestinal phase. The increase in surface roughness and the new characteristic peaks (e.g., Si-O-Si) of aged PS directly accounted for the change in Cd bioaccessibility. The change in organic matter content indirectly accounted for the exceptional increase in Cd bioaccessibility of black soil with aged PS in small intestinal phase. Furthermore, the changes in cation exchange capacity and Cd mobility factor caused by aged PS explained the change in Cd leachability. These results contribute to a deeper understanding about environmental and public health in complicated emerging scenarios.

Differential response of Hg-methylating and MeHg-demethylating microbiomes to dissolved organic matter components in eutrophic lake water.

Methylmercury (MeHg) production in aquatic ecosystems is a global concern because of its neurotoxic effect. Dissolved organic matter (DOM) plays a crucial role in biogeochemical cycling of Hg. However, owing to its complex composition, the effects of DOM on net MeHg production have not been fully understood. Here, the Hg isotope tracer technique combined with different DOM treatments was employed to explore the influences of DOM with divergent compositions on Hg methylation/demethylation and its microbial mechanisms in eutrophic lake waters. Our results showed that algae-derived DOM treatments enhanced MeHg concentrations by 1.42-1.53 times compared with terrestrial-derived DOM. Algae-derived DOM had largely increased the methylation rate constants by approximately 1-2 orders of magnitude compared to terrestrial-derived DOM, but its effects on demethylation rate constants were less pronounced, resulting in the enhancement of net MeHg formation. The abundance of hgcA and merB genes suggested that Hg-methylating and MeHg-demethylating microbiomes responded differently to DOM treatments. Specific DOM components (e.g., aromatic proteins and soluble microbial byproducts) were positively correlated with both methylation rate constants and the abundance of Hg-methylating microbiomes. Our results highlight that the DOM composition influences the Hg methylation and MeHg demethylation differently and should be incorporated into future Hg risk assessments in aquatic ecosystems.

Evaporation Driven Hydrovoltaic Generator Based on Nano-Alumina-Coated Polyethylene Terephthalate Film.

Collecting energy from the ambient environment through green and sustainable methods is highly expected to alleviate pollution and energy problems worldwide. Here, we report a facile and flexible hydrovoltaic generator capable of utilizing natural water evaporation for sustainable electricity production. The generator was fabricated by coating nano-Al2O3 on a twistable polyethylene terephthalate film. An open circuit voltage of 1.7 V was obtained on a piece of centimeter-sized hydrovoltaic generator under ambient conditions. The supercapacitor charged by the hydrovoltaic device can power a mini-motor efficiently. Moreover, by expanding the size or connecting it in series/parallel, the energy output of the generator can be further improved. Finally, the influence factors and the mechanism for power generation were primarily investigated. Electrical energy is produced by the migration of water through charged capillary channels. The environmental conditions, the properties of the solution and the morphology of the film have important effects on the electrical performance. This study is anticipated to offer enlightenment into designing novel hydrovoltaic devices, providing diverse energy sources for various self-powered devices and systems.

Microplastic aging alters the adsorption-desorption behaviors of sulfamethoxazole in marine animals: A study in simulated biological liquids.

Antibiotics and microplastics (MPs) coexisting as unique environmental contaminants may cause unintended environmental issues. In this study, the adsorption-desorption behaviors of sulfamethoxazole (SMX) on both original and UV-aged MPs were examined. Polyhydroxyalkanoates (PHA) and polyethylene (PE), which represent degradable and refractory MPs, respectively, were chosen as two distinct types of MPs. Furthermore, simulated fish intestinal fluids (SFIF) and simulated mammalian stomach fluids (SMGF) were employed to evaluate the desorption behaviors of SMX from aged MPs. Our findings demonstrate that UV-aging altered the polarity, hydrophilicity, and structure of the MPs. Aged MPs showed a higher adsorption capacity than the original MPs and they have a higher desorption capacity than original MPs in simulated body fluids. PE has a higher SMX desorption capacity in SFIF and the opposite happened in SMGF. Our results highlight the importance of considering the different adsorption-desorption behaviors of antibiotics on MPs when evaluating their environmental impact.

Differential effects of nitrate and ammonium on the growth of algae and microcystin production by nitrogen-fixing Nostoc sp. and non-nitrogen-fixing Microcystis aeruginosa.

Cyanotoxins produced by cyanobacteria are a significant threat to human health. However, their responses to nitrogen (N) supplies could differ between N-fixing and non-N-fixing species, which has been poorly understood. This study aimed to compare the responses of the non-N-fixing Microcystis aeruginosa and N-fixing Nostoc sp. to varying concentrations of nitrate and ammonium. This comparison had been conducted by analyzing chlorophyll-a contents, maximum quantum efficiencies of photosystem II, microcystin production, and related gene expressions. Our findings revealed that nitrate substantially stimulated the growth of both M. aeruginosa and Nostoc sp. with biomass increase by 366.2 ± 56.5 and 93.0 ± 14.0%, respectively, at 16 mg-N/L. In contrast, high ammonium concentrations suppressed their growth. Furthermore, the intracellular concentration of microcystins produced by M. aeruginosa was higher under high nitrate. Extracellular microcystins showed an opposite trend to increases in nitrate and ammonium. Ammonium increases the production and releases microcystin from Nostoc sp. N metabolism genes showed a similar trend with toxin formation genes, which were up-regulated under the high N treatments. This study provides valuable insights into the impacts of N supplies on growths of N- and non-N-fixing cyanobacteria, as well as microcystin production, which helps to develop effective strategies for managing cyanobacterial blooms.

A MOF-on-MOF composite encapsulating sensitized Tb(III) as a built-in self-calibrating fluorescent platform for selective sensing of F ions.

The establishment of simple and sensitive detection methods for fluoride ion (F-) is of great importance for its effective prevention and control, and metal-organic framework (MOF) has attracted much attention for sensing applications due to its high surface areas and tunable structures. Herein, we successfully synthesized a fluorescent probe for ratiometric sensing of F- by encapsulating sensitized Tb3+ in a MOF-on-MOF material (UIO66/MOF801, with the formula of C48H28O32Zr6 and C24H2O32Zr6, respectively). We found that Tb3+@UIO66/MOF801 can be used as a built-in fluorescent probe for fluorescence-enhanced sensing of F-. Interestingly, the two fluorescence emission peaks of Tb3+@UIO66/MOF801 at 375 nm and 544 nm exhibit different fluorescence responses to F- under excitation at 300 nm. The 544 nm peak is sensitive to F-, while the 375 nm peak is insensitive to it. Photophysical analysis indicated that the photosensitive substance was formed, which promotes the absorption of 300 nm excitation light by the system. Self-calibrating fluorescent detection of F- was achieved due to the unequal energy transfer toward the two different emission centers. The detection limit of Tb3+@UIO66/MOF801 for F- was 4.029 µM, which is far lower than the WHO guideline for drinking water. Moreover, the ratiometric fluorescence strategy showed a high concentration tolerance of interference, because of its inner-reference effect. This work highlights the high potential of lanthanide ion encapsulated MOF-on-MOF as environmental sensors, and offers a scalable way for construction of the ratiometric fluorescence sensing systems.

Derivation of copper water quality criteria in Bohai Bay for the protection of local aquatic life and the ecological risk assessment.

Developing effective marine water quality criteria (WQC) is crucial for controlling marine contamination and protecting marine life. The WQC for copper is urgently needed due to the toxicity and widespread of copper contamination. In this work, both short-term water quality criteria (SWQC) and long-term water quality criteria (LWQC) under 10 % effect endpoints were derived by using the model averaging of species sensitivity distribution (SSD10) method for Bohai Bay. The WQC values were obtained directly from the hazardous concentration for 5 % of species (HC5) values, which removes the influence of arbitrary assessment factor (AF). Modifications to the acute-chronic ratio (ACR) strategies and the inclusion of the test toxicity data of local species also improved the accuracy and applicability of the WQC values. The derived SWQC and LWQC were 2.21 and 0.45 µg/L, respectively. Furthermore, the overall risk level of copper in Bohai Bay was evaluated by using the risk quotient (RQ) method, and the results showed it was at a moderate-low level. This study provides a new approach for the derivation of the WQC for Cu and the risk assessment of Bohai Bay, which is essential for the protection of local aquatic life and provides guidance to the establishment of the national WQC.

The global research trend on cadmium in freshwater: a bibliometric review.

Cadmium pollution turns out to be a global environmental problem. This study conducted a quantitative and qualitative bibliometric analysis based on 9188 research items from the Web of Science Core Collection published in the last 20 years (2000-2020), presenting an in-depth statistical investigation of global freshwater cadmium research progress and developing trend. Our results demonstrated that the researchers from China, the USA, and India contribute the most to this field. The primary sources of cadmium are mining, industry, wastewater, sedimentation, and agricultural activities. In developing countries, cadmium exposure occurs mainly through the air, freshwater, and food. Fish and vegetables are the main food sources of cadmium for humans because of their high accumulation capability. Source evaluation, detection, and remediation represent the main technologies used to clean up cadmium-contaminated sites. To mitigate the risk of cadmium contamination in freshwater, biomarker-based cadmium monitoring methods and integrated policies/strategies to reduce cadmium exposure merit further concern.

Insight into muscle quality of white shrimp (Litopenaeus vannamei) frozen with static magnetic-assisted freezing at different intensities.

The effects of magnetic field-assisted immersion freezing (MF) with different intensities (20, 40, 60, and 80 mT) on the freezing process and muscle quality of white shrimp (Litopenaeus vannamei) were studied in the present study. The results showed that, compared with immersion freezing (IF), 60 mT MF (MF-60) shortened the total freezing time, reduced thawing loss and cooking loss, and helped to maintain the water holding capacity and texture properties of frozen shrimp samples. In addition, the increase in the L* value of frozen shrimp samples was also inhibited by MF-60. The result of water distribution revealed that MF-60 reduced the mobility and loss of immobilized water and free water. The microstructure of MF-60 was characterized by smaller pores, indicating that MF-60 promoted the generation of fine ice crystals. Overall, MF-60 was beneficial in reducing ice crystal size and inhibiting the loss of shrimp muscle quality loss during the freezing process.

Risks of applying mobilising agents for remediation of arsenic-contaminated soils: Effects of dithionite-EDTA and citric acid on arsenic fractionation, leachability, oral bioavailability/bioaccessibility and speciation.

Arsenic (As) mobilisation assists in remediating As-contaminated soils but might increase ecological and health risks. In this study, risks of applying two mobilising agents were assessed, i.e. an emerging reducing-chelating composite agent [dithionite (Na2S2O4)-EDTA] and a classical low-molecular-weight organic acid (LMWOA) [citric acid (C6H8O7)]. Results showed that both agents induced sharp increase in leachability-based ecological risk of As. Interestingly, the two agents had opposite performances regarding health risks. Na2S2O4-EDTA significantly increased As relative bioavailability (RBA) to 1.83 times that in controls based on in vivo mouse model, and As bioaccessibility to 1.96, 1.65 and 1.20 times in gastric, small intestinal and colon phases based on in vitro PBET-SHIME model. Besides, it caused significant increase of highly toxic As(Ⅲ) in colon fluid. In contrast, C6H8O7 significantly reduced RBA and bioaccessibility of soil As in colon by 44.44% and 14.65%, respectively. Importantly, C6H8O7 restrained bioaccessible As(V) reduction and promoted bioaccessible As(Ⅲ) methylation, further reducing health risk. The phenomena could mainly be attributed to excessive metal components release from soil by C6H8O7 and gut microbiota metabolism of C6H8O7. In summary, C6H8O7 and similar LMWOAs are recommended. The study contributes to mobilising agent selection and development and provides a reference for managing remediation sites.

Distinct responses of Chlorella vulgaris upon combined exposure to microplastics and bivalent zinc.

Microplastics (MPs) and heavy metals are ubiquitous pollutants in the aquatic environment. In this study, the sorption behavior of two typical MPs (PVC and PE) to bivalent zinc ions (Zn(II)) and their combined toxic effects on Chlorella vulgaris were systemically studied. The growth inhibition rate, the activities of photosynthesis and antioxidant enzymes (SOD and CAT), the cell membrane integrity and the cell apoptosis rate were employed to evaluate the toxicity. Our result showed that PVC and PE have different adsorption capacities for Zn(II), and the combined exposure to Zn(II) and MPs had distinct patterns on the inhibition of the cell growth and induction of oxidative stress. Under our experimental concentrations, PE and Zn(II) showed a synergistic effect, while PVC and Zn(II) exhibited an antagonistic effect. Finally, an action mechanism was proposed to explain the experimental phenomena. This study demonstrated that flow cytometry can be a powerful tool to study the toxic effect of MP composites, and MPs can not only allow a free ride for the water contaminants, but also remarkably alter their toxic effects on phytoplankton. These effects deserve further consideration during evaluation of ecological risks of MPs in the water environment.

Dye-encapsulated Zr-based MOFs composites as a sensitive platform for ratiometric luminescent sensing of antibiotics in water.

Overuse of antibiotics posed a global threat to human health and the ecological environment. Efficient detection and control of antibiotic pollution demand novel sensory materials. Here, a dual-luminescent material FS@UIO66 was successfully synthesized by encapsulating fluorescent molecules (fluorescein sodium, FS) in UIO66 based on the in-situ encapsulation method. We found that the dual emission peaks of FS@UIO66 at 369 and 515 nm can be sensitively and synchronously quenched by tetracycline (TET). Interestingly, these two peak intensities were switched anisotropically by levofloxacin (LEV), in which the signal at 515 nm was enhanced. Photophysical analysis revealed that there may exist a competition and replenishment mechanism in the sensing processes. The ratiometric fluorescent feature was employed for rapid detection of TET and LEV, with detection limits of 0.2444 µM and 0.2808 µM for TET and LEV, respectively. The superior sensitivity, high selectivity, and excellent recyclability of FS@UIO66 in sensing TET and LEV were demonstrated in this work. In addition, TET and LEV were also successfully detected by FS@UIO66 in water from real water environment. The results indicate that FS@UIO66 composites are favorable for TET and LEV detection, presenting a great sensing platform for antibiotic detection.

Graphene-Based Membranes for Water Desalination: A Literature Review and Content Analysis.

Graphene-based membranes have unique nanochannels and can offer advantageous properties for the water desalination process. Although tremendous efforts have been devoted to heightening membrane performance and broadening their application, there is still lack of a systematic literature review on the development and future directions of graphene-based membranes for desalination. In this mini-review, literature published between 2011 and 2022 were analyzed by using the bibliometric method. We found that the major contributors to these publications and the highest citations were from China and the USA. Nearly 80% of author keywords in this analysis were used less than twice, showing the broad interest and great dispersion in this field. The recent advances, remaining gaps, and strategies for future research, were discussed. The development of new multifunctional nanocomposite materials, heat-driven/solar-driven seawater desalination, and large-scale industrial applications, will be important research directions in the future. This literature analysis summarized the recent development of the graphene-based membranes for desalination application, and will be useful for researchers in gaining new insights into this field.