ABSTRACT
We examined: 1 group--16 children with chronic gastritis (CG) and autoimmune thyroiditis (AT); 2 group--41 children with functional thyroidal pathologies; 3 group--23 children with CG only. The average age in all groups was 11.17 +/- 2.98. HP (68%) was revealed equally frequently in all the groups. Antibodies to H+/K(+)-ATPase of parietal cells were revealed in the first and second groups only (31-36%), p < 0.05 as compared to the third group. CG of the stomach body was more frequently revealed in the first group using endoscopic and histological tests. The second group had more frequent and more apparent lesions of the antral part. Thus, in case of concomitant diseases of the thyroid gland CG has an autoimmune nature of an inflammation of the mucous coat of the stomach.
Subject(s)
Gastric Mucosa/pathology , Gastritis/complications , Gastritis/pathology , Thyroid Diseases/complications , Thyroid Diseases/pathology , Adolescent , Autoantibodies/analysis , Autoimmune Diseases/immunology , Autoimmune Diseases/pathology , Child , Child, Preschool , Chronic Disease , Female , Gastric Mucosa/immunology , Gastritis/immunology , Gastritis/microbiology , H(+)-K(+)-Exchanging ATPase/immunology , Helicobacter Infections/immunology , Helicobacter Infections/pathology , Helicobacter pylori/isolation & purification , Humans , MaleABSTRACT
54 children at the age of 3-15 suffering from HP-associated CG were examined. Antibodies to H+/K(+)-ATPase of gastric parietal cells were determined with the help of the immune-enzyme analysis method. It was discovered that antibodies to H+/K(+)-ATPase are found in 27.7% of children. Concomitant autoimmune diseases, pangastritis, morphologic symptoms of the pre-atrophic stage of autoimmune gastritis as well as the drop of secretion at the fasting phase were discovered more often in antibody-positive patients. The successful eradication of HP in two children resulted in the disappearance of anti-parietal autoantibodies.
Subject(s)
Autoantibodies/blood , H(+)-K(+)-Exchanging ATPase , Helicobacter Infections/immunology , Helicobacter pylori , Parietal Cells, Gastric/immunology , Adolescent , Autoimmune Diseases/immunology , Child , Child, Preschool , Gastritis, Atrophic/immunology , Gastritis, Atrophic/microbiology , Humans , MaleABSTRACT
Russian and foreign methods used in forensic medicine for detection of the semen in stains on material evidences are compared. The potentialities of quantitative immunofluorescence test for detection of the semen in stains on material evidences, developed at Bureau for Forensic Medical Expert Evaluations of the Leningrad region, are described. Unlike other methods used in Russia, this method detects the semen in stains in the absence of spermatozoa and in stains with very low amount of the semen. Our modification allows objective recording of the results with computer processing. The method is cheaper than its foreign analogs and its sensitivity is similar to them.
Subject(s)
Fluorescent Antibody Technique , Forensic Medicine/methods , Semen/chemistry , Humans , Male , Sensitivity and SpecificityABSTRACT
The efficiency of detection of blood in stains on material evidence by spectrofluorometry and fluorescent hemotest developed at Bureau for Forensic Medical Expert Evaluations of the Leningrad region and by traditional methods used in Russia and abroad is compared. The proposed methods are 1000 times more effective than the methods routinely used in Russia; moreover, they allow objective computer recording and processing. These methods are 50-70 times cheaper than the methods used in foreign countries and are virtually as sensitive. Fluorescent hemotest persuasively proves the presence of blood in stains on material evidence under laboratory conditions, at the site of accident, and even under field conditions (express analysis).