Molecular genetic map construction and QTL analysis of fruit maturation period in grapevine.

In this study, we aimed at finding the genetic regularity of grape maturation period. Early-maturing grapevine, "87-1", was used as the female parent and late-maturing, "9-22", as the male parent, to create an F1 hybrid population. A total of 149 individual plants and their parents were selected as the mapping population. Sequence-related amplified polymorphism and simple-sequence repeat analyses were performed. We performed a linkage analysis and constructed a molecular genetic map. In the obtained map, the female and male parents each covered 19 linkage groups containing 188 and 175 maker loci, respectively. The total map distances for the female and male parents were 1074.5 and 1100.2 cM, respectively, whereas the average genetic distances between each two loci were 5.7 and 7.8 cM, respectively. The interval-mapping method was used in a quantitative trait locus (QTL) analysis for fruit maturation period. A total of 12 QTLs associated with fruit maturation period were detected. These included four QTLs in the male parent genetic map that were located in linkage groups M5, M11, M14-1, and M16, with a 62.6-75.7% rate of contribution of each QTL. Another three QTLs were found in the female parent genetic map, located in linkage groups F6, F14-1, and F18, with a 72.7-77.7% rate of contribution of each QTL. Five more QTLs were detected in the consensus map, located in linkage groups LG11, LG14-1, LG16, LG17, and LG18, with 8.9-75.7% phenotypic variance explained by each QTL.

Quantitative trait locus analysis of grape weight and soluble solid content.

A grapevine hybrid population was derived from a crossing of the early-maturing female parent cultivar '87-1' and the late-maturing male parent cultivar '9-22'. A total of 149 plants were selected from the hybrid population as the mapping population, and after sequence-related amplified polymorphism and simple-sequence repeat marker analysis were conducted we constructed molecular genetic maps of the parents. The molecular linkage map of '87-1' had 19 linkage groups that contained 188 markers, with an average interval of 5.7 cM and a total distance of 1074.5 cM; the '9-22' map had 19 linkage groups that contained 175 markers, with an average interval of 7.8 cM and a total distance of 1100.2 cM. The molecular linkage map of both parents had 19 linkage groups that contained 251 markers, with an average interval of 5.0 cM and a total distance of 1264.2 cM. We used the interval mapping method to conduct a quantitative trait locus (QTL) analysis of grape weight and soluble solid content of the mapping population. Six QTLs were related to grape weight, and the average contribution to the phenotypic variance was between 11.3 and 33.0%. Seven QTLs were related to soluble solid content, and the average contribution to the phenotypic variance was between 15.7 and 55.8%.

Association between GSTM1 polymorphisms and lung cancer: an updated meta-analysis.

The relationship between glutathione S-transferase M1 (GSTM1) genetic polymorphisms and lung cancer has been reported previously. However, the results are not consistent. Therefore, to clarify the association between GSTM1 polymorphisms and lung cancer, we performed a meta-analysis based on published studies. We used the Revman 5.0 software to perform literature retrieval, article selection, data collection, and statistical analysis. We utilized a random-effect model to pool the odds ratios (ORs) and 95% confidence intervals (CIs). A total of 38 eligible studies including 5737 lung cancer patients and 6843 cancer-free control subjects were analyzed. We found no association between GSTM1 genetic polymorphisms and lung cancer risk (OR = 1.15, 95%CI = 0.98-1.36, P = 0.08). Including only Chinese individuals, we found no association between GSTM1 genetic polymorphisms and lung cancer risk (OR = 1.13, 95%CI = 0.97-1.32, P = 0.13). In conclusion, we found that GSTM1 polymorphisms are not associated with lung cancer risk.