ABSTRACT
In the present study, we report the synthesis, electrical and dynamic properties of a new generation bio-based nanocomposite, that is a proton-exchange membrane based on nanocrystalline cellulose (CNC) and imidazole (Im). CNC serves as supporting material and imidazole acts as a proton donor and proton acceptor at the same time. The nanocomposite (1.3 CNC-Im) was synthesized as a film and shows proton conductivity equal to 4.0â¯×â¯10-1â¯S/m at 160⯰C in anhydrous conditions. Analysis of impedance measurements and NMR spectra provided some insight into the macroscopic and microscopic processes involved in proton transport in 1.3 CNC-Im. Local processes such as reorientation of imidazole rings and breaking of hydrogen bonds are identified and their activation energies are calculated. The energies of the macroscopic and microscopic proton transport in CNC-Im film are correlated. The percolation model used confirmed the percolation nature of conductivity in cellulose composites with imidazole.
ABSTRACT
The complex architecture of bone has been investigated for several decades. Some pioneer works proved an existing link between microstructure and external mechanical loading applied on bone. Due to sinuous network of canals and limitations of experimental acquisition technique, there has been little quantitative analysis of three-dimensional description of cortical network. The aim of this study is to provide an algorithmic process, using Python 3.5, in order to identify 3D geometrical characteristics of voids considered as canals. This script is based on micro-computed tomographic slices of two bone samples harvested from the humerus and femur of male cadaveric subject. Slice images are obtained from 2.94⯵m isotropic resolution. This study provides a generic method of image processing which considers beam hardening artefact so as to avoid heuristic choice of global threshold value. The novelty of this work is the quantification of numerous three-dimensional canals features, such as orientation or canal length, but also connectivity features, such as opening angle, and the accurate definition of canals as voids which ranges from connectivity to possibly another intersection. The script was applied to one humeral and one femoral samples in order to analyse the difference in architecture between bearing and non-bearing cortical bones. This preliminary study reveals that the femoral specimen is more porous than the humeral one whereas the canal network is denser and more connected.
Subject(s)
Cortical Bone/ultrastructure , Femur/anatomy & histology , Humerus/anatomy & histology , Imaging, Three-Dimensional/methods , X-Ray Microtomography/methods , Cortical Bone/anatomy & histology , Cortical Bone/diagnostic imaging , Femur/diagnostic imaging , Humans , Humerus/diagnostic imaging , Image Processing, Computer-Assisted/methods , Male , Porosity , Stress, MechanicalABSTRACT
The recommendation from the International Commission on Radiological Protection that the occupational equivalent dose limit for the lens of the eye should be reduced to 20 mSv year(-1), averaged over 5 years with no year exceeding 50 mSv, has stimulated a discussion on the practicalities of implementation of this revised dose limit, and the most appropriate risk and protection framework to adopt. This brief paper provides an overview of some of the drivers behind the move to a lower recommended dose limit. The issue of implementation in the medical sector in the UK has been addressed through a small-scale survey of doses to the lens of the eye amongst interventional cardiologists and radiologists. In addition, a mechanistic study of early and late post-irradiation changes in the lens of the eye in in-vivo-exposed mice is outlined. Surveys and studies such as those described can contribute to a deeper understanding of fundamental and practical issues, and therefore contribute to a robust evidence base for ensuring adequate protection of the eye while avoiding undesirable restrictions to working practices.
Subject(s)
Eye Diseases/etiology , Lens, Crystalline/radiation effects , Occupational Exposure , Ophthalmology , Optometry , Radiation Injuries/etiology , Animals , Eye Diseases/pathology , Eye Diseases/physiopathology , Humans , Mice , Radiation Dosage , Radiation Injuries/pathology , Radiation Injuries/physiopathology , Risk , United KingdomABSTRACT
Sorafenib is a multi-kinase inhibitor that blocks cell proliferation and angiogenesis. It is currently approved for advanced hepatocellular and renal cell carcinomas in humans, where its major mechanism of action is thought to be through inhibition of vascular endothelial growth factor and platelet-derived growth factor receptors. The purpose of this study was to determine whether pixel-by-pixel analysis of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) is better able to capture the heterogeneous response of Sorafenib in a murine model of colorectal tumor xenografts (as compared with region of interest analysis). MRI was performed on a 9.4 T pre-clinical scanner on the initial treatment day. Then either vehicle or drug were gavaged daily (3 days) up to the final image. Four days later, the mice were again imaged. The two-compartment model and reference tissue method of DCE-MRI were used to analyze the data. The results demonstrated that the contrast agent distribution rate constant (K(trans)) were significantly reduced (p <â 0.005) at day-4 of Sorafenib treatment. In addition, the K(trans) of nearby muscle was also reduced after Sorafenib treatment. The pixel-by-pixel analysis (compared to region of interest analysis) was better able to capture the heterogeneity of the tumor and the decrease in K(trans) four days after treatment. For both methods, the volume of the extravascular extracellular space did not change significantly after treatment. These results confirm that parameters such as K(trans), could provide a non-invasive biomarker to assess the response to anti-angiogenic therapies such as Sorafenib, but that the heterogeneity of response across a tumor requires a more detailed analysis than has typically been undertaken.
Subject(s)
Angiogenesis Inhibitors/therapeutic use , Colorectal Neoplasms/diagnosis , Contrast Media , Magnetic Resonance Imaging , Niacinamide/analogs & derivatives , Phenylurea Compounds/therapeutic use , Protein Kinase Inhibitors/therapeutic use , Angiogenesis Inhibitors/administration & dosage , Animals , Colorectal Neoplasms/drug therapy , Disease Models, Animal , Humans , Image Processing, Computer-Assisted , Mice , Neovascularization, Pathologic/drug therapy , Niacinamide/administration & dosage , Niacinamide/therapeutic use , Phenylurea Compounds/administration & dosage , Protein Kinase Inhibitors/administration & dosage , Sorafenib , Transplantation, HeterologousABSTRACT
The present systematic review was performed under the auspices of the European Lung Cancer Working Party (ELCWP) in order to determine the role of early intermediate criteria (surrogate markers), instead of survival, in determining treatment efficacy in patients with lung cancer. Initially, the level of evidence for the use of overall survival to evaluate treatment efficacy was reviewed. Nine questions were then formulated by the ELCWP. After reviewing the literature with experts on these questions, it can be concluded that overall survival is still the best criterion for predicting treatment efficacy in lung cancer. Some intermediate criteria can be early predictors, if not surrogates, for survival, despite limitations in their potential application: these include time to progression, progression-free survival, objective response, local control after radiotherapy, downstaging in locally advanced nonsmall cell lung cancer (NSCLC), complete resection and pathological TNM in resected NSCLC, and a few circulating markers. Other criteria assessed in these recommendations are not currently adequate surrogates of survival in lung cancer.
Subject(s)
Biomarkers, Tumor/metabolism , Biomarkers/metabolism , Carcinoma, Non-Small-Cell Lung/blood , Carcinoma, Non-Small-Cell Lung/diagnosis , Lung Neoplasms/blood , Lung Neoplasms/diagnosis , Medical Oncology/standards , Pulmonary Medicine/methods , Carcinoma, Non-Small-Cell Lung/surgery , Carcinoma, Non-Small-Cell Lung/therapy , Disease-Free Survival , Europe , Evidence-Based Medicine , Guidelines as Topic , Humans , Lung Neoplasms/surgery , Lung Neoplasms/therapy , Medical Oncology/methods , Treatment OutcomeABSTRACT
The present paper focuses on the mechanical behavior analysis of bones at mesoscopic scale, paying a special attention to the trabecular bone and the bone marrow filling the porosities. Uni-axial quasi-static compression tests under unconfined conditions have been performed to identify the mechanical behavior of 46 trabecular bone samples. The bone marrow for 22 samples has been preserved to analyze the fluid flow effects on the crushing response. Although deformation patterns do not differ significantly, the average crush behavior of the trabecular bone shows an unexpected decrease of the mechanical properties when the marrow is kept in the sample (26% for the elastic modulus (E(a)), 38% for the maximum compressive stress (σ(max)) and 33% for the average stress (σ(mean))). An explanation is given by analyzing the contribution of the bone marrow viscosity which smooths the mechanical response. A numerical analysis on an idealized trabecula confirms that the marrow induces transverse pressure and extra local stress on trabeculae during its flow, causing the premature collapse of the trabecular network.
Subject(s)
Bone Marrow/physiology , Compressive Strength , Materials Testing , Ribs/physiology , Biomechanical Phenomena , Finite Element Analysis , Stress, MechanicalABSTRACT
PURPOSE: Prior non invasive ventilation (NIV) is associated with an increased mortality in patients with haematological malignancies and acute respiratory failure treated by invasive mechanical ventilation (IMV). We have assessed whether NIV failure is an independent prognostic factor for hospital discharge in a general cancer population treated by IMV. METHODS: 106 patients with solid tumors and 58 patients with haematological malignancies were eligible for this retrospective study; 41 were treated by NIV before IMV. RESULTS: The main indications for mechanical ventilation were sepsis/shock (35%), acute respiratory failure (33%), cardiopulmonary resuscitation (16%) and neurologic disease (10%). Respectively, 35%, 28% and 24% of the patients were extubated, discharged from the intensive care unit (ICU) and from the hospital. For patients treated with NIV prior to IMV, the rates were 22%, 17% and 10%, respectively. In multivariate analysis, 3 variables were independently associated with a decreased probability of being discharged from the hospital: NIV use before IMV (odds ratio/OR=0.30, 95% confidence interval/CI: 0.09-0.95; p=0.04); leukopenia (OR=0.21, 95% CI: 0.06-0.77; p=0.02) and serum bilirubin >1.1 mg/dl (OR=0.38, 95% CI: 0.16-0.94; p=0.04). CONCLUSION: NIV failure before IMV is an independent poor prognostic factor in cancer patients treated by IMV.
Subject(s)
Neoplasms/mortality , Respiration, Artificial/adverse effects , Adult , Aged , Aged, 80 and over , Bilirubin/blood , Female , Humans , Leukopenia/complications , Male , Middle Aged , Prognosis , Retrospective StudiesABSTRACT
OBJECTIVE: To assess the effect of continuous venovenous hemodiafiltration (CVVHDF) in cancer patients with acute renal failure. PATIENTS AND METHODS: Retrospective study of all patients with acute renal failure requiring dialysis and treated with CVVHDF in a medical intensive care unit (ICU) from a cancer hospital. RESULTS: From January 1997 until December 2002, 32 cancer patients were treated with CVVHDF for acute renal failure. Their characteristics were: male/female 23/9, median age 61 years, haematological/solid tumours 16/16, and median APACHE II and IGS II scores 31/67. The number of organ failures was 1/2/3/4 in respectively 10/6/13/2 patients. Complete, partial or absence of resolution of acute renal failure was noted in 13, 8 and 11 patients. Sixteen patients (50%) died in the ICU and 15 (47%) were discharged alive from the hospital. In univariate analysis, variables statistically significantly adversely associated with hospital mortality were renal failure of renal origin, bone marrow transplant, increasing number of organ failures, reduced lymphocyte count, elevated bilirubin and lower creatinine levels, increased thromboplastin time, younger age, increased APACHE II and IGS II, ARDS and mechanical ventilation. In multivariate analysis, two models were used including either APACHE II or IGS II. The number of organ failures was found as the only significant prognostic factor in both models ( p=0.01). Elevated phosphate level was a poor prognostic factor for hospital mortality ( p=0.04) in the model including APACHE II. CONCLUSIONS: In the experience of a single centre, CVVHDF is effective in the treatment of acute renal failure in cancer patients. The increasing number of organ failures was the single independent poor predictive factor for hospital mortality. Cancer characteristics and general gravity scores were not predictive factors.
Subject(s)
Hemofiltration/methods , Neoplasms/complications , Renal Insufficiency/therapy , Adult , Aged , Aged, 80 and over , Belgium/epidemiology , Female , Hospital Mortality , Humans , Intensive Care Units , Male , Middle Aged , Prognosis , Prospective Studies , Renal Insufficiency/complicationsABSTRACT
The objective of this retrospective study of prospectively registered patients was to determine the usefulness and efficacy of noninvasive ventilation (NIV) in cancer patients admitted to the medical intensive care unit of an European cancer hospital for a medical complication, as reflected in discharges from the intensive care unit (ICU) and from hospital. The subjects were a total of 40 consecutive cancer patients (28 with solid tumours and 12 with haematological malignancies) who required immediate or delayed NIV. Variables relating to demographic parameters, severity scores, cancer characteristics, intensive care data and hospital discharge were recorded. The complications making NIV necessary were hypoxaemic pneumonia in 32.5%, hypercapnic ventilatory failure in 30%, multifactorial respiratory failure in 17.5%, acute haemodynamic oedema in 10%, acute respiratory distress syndrome in 2.5%, alveolar haemorrhage in 2.5%, pulmonary embolism in 2.5% and lysis pneumopathy in 2.5%. Most of the patients, 57.5% and 42.5%, respectively, were discharged from the ICU and from the hospital. Among the 10 patients (25%) who required salvage invasive mechanical ventilation, only 1 was discharged from hospital. Sixty-four per cent of the solid tumour patients and 42% of those with haematological malignancies were discharged from the ICU and 50% and 25%, respectively, from the hospital. NIV thus appears to be an effective form of ventilatory support for cancer patients, including those with solid tumours.
Subject(s)
Neoplasms/complications , Respiration, Artificial , Adult , Aged , Aged, 80 and over , Female , Humans , Intensive Care Units , Male , Middle Aged , Patient Discharge/statistics & numerical data , Respiration, Artificial/methods , Respiration, Artificial/statistics & numerical data , Retrospective Studies , Severity of Illness IndexABSTRACT
OBJECTIVE: To validate and compare two severity scoring systems, the Acute Physiology and Chronic Health Evaluation (APACHE) II and Simplified Acute Physiology Score (SAPS) II and to determine their prognostic value for mortality during the hospital stay and after discharge in a specific group of cancer patients admitted to intensive care unit (ICU) for an acute medical complication. DESIGN: Prospective cohort study. SETTING: The medical ICU of a European cancer hospital. SUBJECTS: A total of 261 consecutive cancer patients admitted to ICU for an acute medical complication. MEASUREMENTS: Variables included into the APACHE II and SAPS II scores, as well as characteristics of the cancer, were collected during the first 24 hrs of the ICU stay. Hospital and in-ICU mortalities, overall survival, and survival after day 30 were measured. RESULTS: Observed hospital and ICU mortalities were 33% and 23%. Median survival time was 94 days and 1-yr survival rate was 23%. The mean predicted risk of death was 26.5% with APACHE II and 26.1% with SAPS II. Correlation between both systems was excellent. Calibration for mortality prediction ability of both scoring systems was similar. Discrimination between survivors and nonsurvivors was superior with SAPS II according to the area under the receiver operating characteristic curve but was better with APACHE II for survivors using thresholds minimizing the overall misclassification rates. Multivariate prognostic analysis showed that the scoring systems were the only significant factors for hospital and in-ICU mortalities, whereas characteristics related to the cancer (extent, phase) were the factors predicting survival after discharge. CONCLUSION: The prognosis of cancer patients admitted to ICU for a medical problem is first determined by the acute physiologic changes induced by the complication, as evaluated by the severity scores. There is no major difference between the two assessed scoring systems. They are, however, not accurate enough to be used in the routine management of these patients. After recovery from complications, characteristics related to the neoplastic disease, however, retrieve their independent influence on the further survival.
Subject(s)
APACHE , Neoplasms/complications , Neoplasms/diagnosis , Severity of Illness Index , Acute Disease , Adolescent , Adult , Aged , Aged, 80 and over , Follow-Up Studies , Humans , Intensive Care Units , Middle Aged , Prognosis , Prospective StudiesABSTRACT
Gemcitabine has been demonstrated active in non-small cell lung cancer (NSCLC). The objective of this trial was to evaluate the feasibility of combinations of gemcitabine (1 g/m2 dl,8,15) with cisplatin (60 mg/m2 dl) and carboplatin (200 mg/m2 dl) (CCG; n = 12) or ifosfamide (4.5 g/m2 dl) (IG;n = 4) in patients with advanced NSCLC, in order to prepare a phase III randomised trial. Toxicity, mainly haematological, was tolerable. It consisted in neutropenia (IG) and both thrombopenia and neutropenia (CCG). The administration of carboplatin according to the AUC (AUC = 3) resulted in a significant reduction of haematological toxicity. A good number of responses were documented. These acceptable results urged our group to compare these regimens to the combination of cisplatin, carboplatin and if osfamide.
Subject(s)
Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Carcinoma, Non-Small-Cell Lung/drug therapy , Lung Neoplasms/drug therapy , Antineoplastic Combined Chemotherapy Protocols/adverse effects , Carboplatin/administration & dosage , Carboplatin/adverse effects , Cisplatin/administration & dosage , Cisplatin/adverse effects , Deoxycytidine/administration & dosage , Deoxycytidine/adverse effects , Deoxycytidine/analogs & derivatives , Female , Glomerular Filtration Rate/drug effects , Humans , Ifosfamide/administration & dosage , Ifosfamide/adverse effects , Male , Pilot Projects , GemcitabineSubject(s)
Catheterization, Central Venous/instrumentation , Catheters, Indwelling/standards , Blood Specimen Collection , Catheterization, Central Venous/adverse effects , Catheterization, Central Venous/methods , Catheters, Indwelling/adverse effects , Humans , Infection Control , Neoplasms/drug therapy , Patient SelectionABSTRACT
A prospective collection of positive antimicrobial cultures was performed over 12 consecutive months in the medical intensive care unit of a cancer hospital. In all, 144 infections and 163 pathogens were documented during 87 of the 528 admissions. Lung, urinary, ENT (ear, nose and throat) infections and bacteraemia were the most frequently documented. Staphylococcus species, Streptococcus species, Escherichia coli, Klebsiella species and Pseudomonas species were the most common pathogens. Gram-positive strains were observed predominantly during monomicrobial bacteraemia (48.9%). Methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus epidermidis (MRSE) were found in 58% and 92% of the isolated strains respectively. No particular outbreak was identified. A further prospective study will be necessary to evaluate the impact of the antibiotic use on the selection of resistant strains in our ICU.
Subject(s)
Cancer Care Facilities , Cross Infection/epidemiology , Intensive Care Units/statistics & numerical data , Adult , Bacterial Infections/epidemiology , Bacterial Infections/microbiology , Candidiasis/epidemiology , Cross Infection/microbiology , Herpesviridae Infections/epidemiology , Humans , Methicillin Resistance , Prevalence , Prospective Studies , Staphylococcal Infections/epidemiologyABSTRACT
Overexpression of even non-toxic proteins in bacteria causes a starvation-like response: the arrest of bacterial proliferation and apoptotic-like suicidal cell death. We have shown here that, as in the cells of higher organisms, these effects are accompanied by DNA degradation. The fusion with the bacterial MBP of a polypeptide, belonging to the 14-3-3 family and normally expressed in pumpkin (C. pepo), modifies the apoptotic-like effects of overexpression of this protein in E. coli. Fusion of the full length 14-3-3 protein with the MBP considerably slows down the DNA degradation caused by overexpression of the unmodified MBP. Overexpression of the construct containing a truncated version of the 14-3-3 polypeptide causes immediate arrest of bacterial growth and rapid degradation of the chromosomal DNA. This result suggests that the DNA degradation in bacteria is an active process which can be modified to some extent by an endogenous protein.
ABSTRACT
The goal of this study was to determine the effectiveness and potential indications of cardiopulmonary resuscitation (CPR) in medical cancer patients. A retrospective analysis of the records of patients admitted between November 1985 and January 1992 in the medical ICU of a cancer hospital and having had cardiac arrest was performed. Cardiac arrest occurred in 49 cancer patients. CPR was successful in 19 (39%), but only 5 (10%) were discharged alive from the hospital. CPR was successful in all eight patients in which cardiac arrest was the consequence of an acute cardiovascular drug toxicity, even if cancer was metastatic and treatment intent not curative, while it was effective in only 25% of those in which cardiac arrest was an ultimate complication of various problems, like septic shock or respiratory failure complicating the neoplastic disease. In cancer as in other types of disease, CPR is mainly indicated when cardiac arrest is the consequence of and acute insult.
Subject(s)
Heart Arrest/therapy , Neoplasms/complications , Resuscitation , Adult , Aged , Antineoplastic Agents/adverse effects , Cancer Care Facilities , Female , Follow-Up Studies , Heart Arrest/etiology , Heart Arrest/mortality , Humans , Intensive Care Units , Male , Middle Aged , Neoplasms/mortality , Retrospective Studies , Survival Analysis , Treatment OutcomeABSTRACT
Intensive care is increasingly frequently used in the management of cancer patients. In order to determine whether and how critical-care facilities are used specifically for these patients, we performed a world-wide inquiry in anticancer centres. We mailed a questionnaire to 141 centres and received 84 responses (57.5%). There was at least one oncological intensive care-unit (ICU) in 59 hospitals (70%). The majority of the ICU were found in the European institutions. Data were obtained concerning the ICU organisation, the medical team, the nursing staff, the critical-care techniques and the anticancer treatments performed. Medical ICU appeared to be managed by physicians and nurses often qualified in both intensive care and oncology. These data suggest that oncological intensive care should be part of the training of oncologists and intensivists.
Subject(s)
Cancer Care Facilities , Critical Care , Medical Oncology , Humans , International Cooperation , Medical Staff , Neoplasms/therapy , Surveys and QuestionnairesABSTRACT
We have isolated the nuclear matrices from Pisum sativum cell nuclei using three methods: i. standard procedure involving extraction of cell nuclei with 2 M NaCl and 1% Triton X-100; ii. the same with pretreatment of cell nuclei with 0.5 mM CuSO4 (stabilisation step); and iii. method including lithium diiodosalicylate extraction. We compared the polypeptide pattern and residual DNA content of the nuclear matrices isolated. The nuclear matrices displayed a specific endonuclease activity which was due to the presence of a 32 kDa protein. The isolated nuclear matrices bound specifically the scaffold-attached (SAR) DNA derived from human beta interferon gene, in the exogenous SAR binding assay. Using the DNA-protein binding blot assay we demonstrated the presence of two nuclear matrix proteins of 66 kDa and 62 kDa which bound specifically SAR DNA.
Subject(s)
DNA/isolation & purification , DNA/metabolism , Nuclear Proteins/isolation & purification , Nuclear Proteins/metabolism , Pisum sativum/metabolism , Antigens, Nuclear , Cell Nucleus/chemistry , Consensus Sequence , DNA-Binding Proteins/metabolism , Deoxyribonuclease I/chemistry , Electrophoresis, Agar Gel , Electrophoresis, Polyacrylamide Gel , Humans , Interferon-beta/genetics , Nuclear Matrix/chemistry , Plasmids/metabolismABSTRACT
The nuclear matrices from White bush (Cucurbita pepo var. patisonina) cell nuclei have been isolated using three methods: I, standard procedure involving extraction of cell nuclei with 2 M NaCl and 1% Triton X-100; II, the same with pre-treatment of cell nuclei with 0.5 mM CuSO4 (stabilisation step); and III, method with extraction by lithium diiodosalicylate (LIS), and compared the polypeptide pattern. The isolated matrices specifically bind SAR DNA derived from human beta-interferon gene in the exogenous SAR binding assay and in the gel mobility shift assay. Using IgG against the 32 kDa endonuclease we have found in the DNA-protein blot assay that this protein is one of the proteins binding SAR DNA. We have identified three proteins with molecular mass of 65 kDa, 60 kDa and 32 kDa which are responsible for SAR DNA binding in the gel mobility shift assay experiments.