Effects of Soil Conditions on Root Rot of Soybean Caused by Fusarium graminearum.

Fusarium graminearum is an important soybean pathogen that causes seedling disease, root rot, and pre- and postemergence damping-off. However, effects of soil conditions on the disease are not well understood. The objective of this greenhouse study was to determine the impacts of soil texture, pH, and soil moisture on seedling root rot symptoms and detrimental effects on seedling development caused by F. graminearum. F. graminearum-infested millet was added (10%, vol/vol) to soil with four different textures (sand, loamy sand, sandy loam, and loam). Soil moisture was maintained at saturation, field capacity or permanent wilting point at soil pH levels of 6 or 8. Seedlings were evaluated 4 weeks after planting for root rot, root length, root and shoot dry weights, leaf area, and F. graminearum colonization (by qPCR). There was a significant interaction between soil moisture and soil texture for root rot assessed visually (P < 0.0001). Highest severity (67%) and amount of F. graminearum DNA were observed at pH 6 and permanent wilting point in sandy loam soils. Pot saturation resulted in the lowest levels of disease in sandy loam and loam soils (11.6 and 10.8%, respectively). Reductions in seedling growth parameters, including root length, foliar area, shoot and root dry weights, and root tips, relative to the noninfested control, were significantly greater in sandy loam soils. In contrast, there were no significant growth reductions in sand. This study showed that levels of root rot increased under moisture-limiting conditions, producing detrimental effects on plant development.

Dissoconiaceae associated with sooty blotch and flyspeck on fruits in China and the United States.

Zasmidium angulare, a novel species of Mycosphaerellaceae, and several novel taxa that reside in Dissoconiaceae, were identified from a collection of apples and Cucurbita maxima (cv. Blue Hubbard) from China and the USA that exhibited sooty blotch and flyspeck (SBFS) signs on their host substrata. Morphology on fruit surfaces and in culture, and phylogenetic analyses of the nuclear ribosomal DNAs 28S and internal transcribed spacer regions, as well as partial translation elongation factor 1-alpha gene sequences in some cases, were used to delineate seven previously unidentified species and three known species. Pseudoveronaea was established as a new genus of Dissoconiaceae, represented by two species, P. ellipsoidea and P. obclavata. Although Pseudoveronaea was morphologically similar to Veronaea, these fungi clustered with Dissoconiaceae (Capnodiales) rather than Chaetothyriales (Herpotrichiellaceae). Ramichloridium mali comb. nov., and three novel species, R. cucurbitae, R. luteum and R. punctatum were closely related with R. apiculatum, which together formed a distinct subclade in Dissoconiaceae. Species of Dissoconium s.lat. clustered in two well-supported clades supported by distinct morphological and cultural features. Subsequently Uwebraunia, a former synonym of Dissoconium, was resurrected for the one clade, with new combinations proposed for U. australiensis, U. commune, U. dekkeri and U. musae. Furthermore, we also reported that D. aciculare, Dissoconium sp., U. commune and U. dekkeri were associated with SBFS on apples.

Temporal patterns in appearance of sooty blotch and flyspeck fungi on apples.

Sooty blotch and flyspeck (SBFS) is a complex of about 80 fungal species that blemish the surface of apple fruit in humid regions worldwide. The dark colonies become visible in mid- to late summer, reducing the value of fresh fruit. Although many SBFS species can co-occur in the same orchard and even on the same apple, little is known about temporal patterns of these species, including the timing of colony appearance. To test the hypothesis that colonies of SBFS species appear on apples at characteristic times during the growing season, 50 apples were monitored weekly at three Iowa orchards in 2006 and six orchards in 2007 and 2008. However, a mean of 24.3 apples per orchard was assessed at harvest because of apple drop throughout the season. Colonies were marked with colored pens as they appeared. After harvest and after storage of apples at 2 °C for 3 months, SBFS colonies on each fruit were counted and classified by morphology, and a representative subset of colonies was excised from the fruit and preserved on dried peels for species identification using rDNA. Seventeen species were identified. Stomiopeltis spp. RS1 and RS2 appeared on apples 10 to 14 days before other SBFS taxa. Dissoconium aciculare was generally the last species to appear on apple fruit, and it continued to appear during postharvest storage. The most prevalent taxa in Iowa orchards were also the most abundant. Diversity of SBFS fungi in an orchard was positively correlated with cumulative hours of surface wetness hours due to rainfall or dew, which is believed to favor growth of SBFS fungi. Species-specific information about temporal patterns of appearance on apple fruit may lead to improved SBFS management strategies.