Seasonal variations in integumental glycoconjugates of Rana rugosa.

BACKGROUND: Although environmental conditions can influence the expression of glycoconjugates (GCs) in the epidermis and cutaneous gland of amphibians, seasonal features regarding GCs in frogs have rarely been studied. In the present study, we report the seasonal variations of the integumental GCs in Rana rugosa. METHODS: GCs were investigated at the light microscopic level using conventional and lectin histochemistry. RESULTS: During the pre- through posthibernating periods, stronger periodic acid-Schiff (PAS)-positive GCs were observed in the stratum corneum and transitional layer of the epidermis. At the same time, alcian blue and PAS-positive GCs filled the lumen of the mucous gland. The affinity for PNA in the transitional layer, UEA-1 in stratum germinativum, and SBA in the mucous glands increased significantly during the same periods. In addition, new GCs, which are specific for SBA in the stratum corneum, BSL-1, RCA-1, sWGA, and LCA in some cells of the transitional layer, were detected. CONCLUSIONS: Elevated GCs and a diversity of lectin affinities may be a result of seasonal adaptations related to hibernation. An affinity for PNA, UEA-1, and SBA in the frog skin may be useful as a marker of seasonal changes.

Simulated hibernation of sea turtles in the laboratory: I. Feeding, breathing frequency, blood pH, and blood gases.

Captive immature green (Chelonia mydas) and Kemp's ridley (Lepidochelys kempi) sea turtles were examined to determine if a hibernation-like state could be induced under controlled conditions. Both species demonstrated that they are able to acclimate to cold temperatures behaviorally. However, the two species appeared to respond differently to decreasing temperature. Whereas the green turtles tolerated the onset of cold water temperatures by reducing swimming activity, the ridleys became very agitated and active as they were exposed to temperatures below 20 degrees C. Nevertheless, both species displayed semi-dormant behavior at temperatures below 15 degrees C, coming to the surface to breathe periodically at intervals of up to three hours. At low temperatures, venous blood pO2 and pCO2 decreased, whereas venous blood pH increased. Feeding also decreased as either species was exposed to cold temperature: greens (at 15 degrees C) and ridleys (at 20 degrees C) decreased food consumption to 50% of control levels, and ceased feeding below 15 degrees C. Thus, these species tolerated temperature drops and the associated hypophagia. They did not exhibit cold-stunning behavior, as has been observed in wild sea turtles exposed to rapid temperature drops, or prolonged periods of hibernation-like dormancy, as has been proposed for wild sea turtles during cold winter months.