ABSTRACT
Medicinal plants contain a large variety of chemical compounds in highly variable concentrations, so the quality control of these materials is especially complex. With this purpose, regulatory institutions have accepted chromatographic fingerprints as a valid tool to perform the analyses. In order to improve the results, separation conditions that maximise the number of detected peaks in these chromatograms are needed. This work reports the extension of a simulation strategy, based on global retention models previously developed for selected compounds, to all detected peaks in the full chromatogram. Global models contain characteristic parameters for each component in the sample, while other parameters are common to all components and describe the combined effects of column and solvent. The approach begins by detecting and measuring automatically the position of all peaks in a chromatogram, obtained preferably with the slowest gradient. Then, the retention time for each detected component is fitted to find the corresponding solute parameter in the global model, which leads to the best agreement with the measured experimental value. The process is completed by developing bandwidth models for the selected compounds used to build the global retention model based on gradient data, which are applied to all peaks in the chromatogram. The usefulness of the simulation approach is demonstrated by predicting chromatographic fingerprints for three medicinal plants with specific separation problems (green tea, lemon balm and linden), using several multi-linear gradients that lead to problematic predictions.
Subject(s)
Melissa , Plants, Medicinal , Tea/chemistry , Tilia , Plants, Medicinal/chemistry , Plant Extracts/chemistry , Chromatography, High Pressure Liquid/methodsABSTRACT
In liquid chromatography, the reliability of predictions carried out with retention models depends critically on the quality of the training experimental design. The search of the best design is more complex when gradient runs are used instead of isocratic experiments. In Part I of this work (JCA 1624 (2020) 461180), a general methodology based on the error propagation theory was developed and validated for assessing the quality of training designs involving gradients. The treatment relates the mathematical properties of a retention model with the geometry of the training designs and their subsequent predictions. In that work, only five usual designs were considered. Part II investigates in detail the effects on predictions when the features of the training design (number and distribution of the experiments, initial and final modifier content, gradient slope(s), and location of gradient nodes and pulses) are varied systematically. Several groups of related designs containing one or more isocratic steps, linear or multi-linear gradients, or mixed isocratic/gradient runs, among others (in total 38 designs) were evaluated. Box and whiskers and triple plots of expected relative uncertainties were used to evidence the differences in prediction performance. The purpose was to give recommendations to construct designs with good prediction performance. The best designs sample (considering all runs) concentrations as diverse as possible, at any gradient time.
Subject(s)
Chromatography, Liquid , Models, Chemical , Reproducibility of Results , Research DesignABSTRACT
The resolution of samples containing unknown compounds of different nature, or without standards available, as is the case of chromatographic fingerprints, is still a challenge. Possibly, the most problematic aspect that prevents systematic method development is finding models that describe without bias the retention behaviour of the compounds in the samples. In this work, the use of global models (able to describe the whole sample) is proposed as an alternative to the use of individual models for each solute. Global models contain parameters that are specific for each solute, while other parameters ârelated to the column and solventâ are common for all solutes. A special regression procedure is presented for the construction of global models, which are applied to predict highly complex chromatograms, such as chromatographic fingerprints, for diverse experimental conditions in isocratic and gradient elution. Another interesting application is the prediction of molecular properties, such as log Po/w, from the specific solute parameters of the global models. The examined adapted models are based on the equations proposed by Snyder, Schoenmakers, Neue and Kuss, Jandera, and Bosch Rosés to describe the retention. In all cases, the predictive capability was very satisfactory. Two cases of study were considered: chromatograms of camomile extracts analysed using acetonitrile gradients, and a set of 145 known compounds in a wide range of structures and functionalities, eluted isocratically with acetonitrile/water mobile phases.
Subject(s)
Chromatography/methods , Models, Theoretical , Algorithms , Chamomile/chemistry , Computer Simulation , Plant Extracts/chemistry , Reference Standards , Regression Analysis , Sulfonamides/chemistry , Time Factors , Water/chemistryABSTRACT
The basis of interpretive optimisation in liquid chromatography is the prediction of resolution, from appropriate solute retention models. The reliability of the process depends critically on the quality of the experimental design. This work develops, validates and applies a general methodology aimed to evaluate the quality of any training experimental design, which will be applied in Part II to design optimisation. The methodology is based on the systematic evaluation of the uncertainties associated to the prediction of retention times in comprehensive scans of both isocratic and gradient experimental conditions. It is able to evaluate comprehensively experimental designs of arbitrary complexity. Five common training experimental designs were used to model the retention, according to the Linear Solvent Strength (LSS) and the Neue-Kuss (NK) equations, using a set of 14 sulphonamides of different polarity. The results are presented in terms of relative uncertainties in predictions, which provide significant and interpretable results. The magnitude of such uncertainties, together with the systematic, coherent and logical changes observed at increasing solute hydrophobicity, give support to the results. The NK model gave smaller errors and unbiased predictions, whereas the LSS model gave rise to lack of fit. Isocratic training designs, which are widely accepted as the most informative, are confirmed as the best. As a general conclusion, gradients are predicted with intrinsically smaller uncertainties, independently of the training experimental design. In addition, gradients are more insensitive than isocratic predictions with regard to the type of training design used. Isocratic predictions deteriorate quickly with mobile phase composition. This explains the better performance of gradient predictions, even with biased models.
Subject(s)
Chromatography, Liquid/methods , Hydrophobic and Hydrophilic Interactions , Research Design , Solvents/chemistry , Sulfonamides/chemistryABSTRACT
The development of a new comprehensive two-dimensional liquid chromatographic method is described, to obtain the profiles of polyphenolic compounds present in olive (Olea europaea L.) leaves and pulps from different genetic origin. Optimisation of the stationary phase nature, particle size, column length and internal diameter, as well as other separation conditions, was performed. Along the study, three stationary phases (C18, PFP and phenyl) in the first dimension (1D), and five (C18, amide, cyano, phenyl and PFP) in the second dimension (2D) were combined to obtain the maximal number of resolved peaks. The optimised method successfully characterised the presence of 26and 29 common polyphenols in olive leaves and pulp extracts, respectively. Peak volume ratios were used to develop linear discriminant analysis models able to distinguish olive leaves and pulp extracts among seven cultivars from several Spanish regions. The results demonstrate that polyphenolic profiles were characteristic of each cultivar.
Subject(s)
Olea/chemistry , Plant Extracts/chemistry , Polyphenols/chemistry , Chromatography, High Pressure Liquid/methods , Plant Leaves/chemistryABSTRACT
Micellar liquid chromatography (MLC) is a reversed-phase mode with mobile phases containing an organic solvent and a micellised surfactant. Most procedures developed in MLC are implemented in the isocratic mode, since the general elution problem in chromatography is less troublesome. However, gradient elution may be still useful in MLC to analyse mixtures of compounds within a wide range of polarities, in shorter times. MLC using gradients is attractive to determine by direct injection moderate to low polar compounds in physiological samples. In these analyses, the use of initial micellar conditions (isocratic or gradient) with a fixed amount of surfactant above the critical micellar concentration, keeping the organic solvent content low, will provide better protection of the column against the precipitation of the proteins in the physiological fluid. Once the proteins are swept away, the elution strength can be increased using a positive gradient of organic solvent to reduce the analysis time. This may give rise to the transition from the micellar to the submicellar mode, since micelles are destroyed at sufficiently high concentration of organic solvent. In this work, several retention models covering extended solvent domains in MLC are developed and tested, and applied to investigate the performance in isocratic, linear and multi-linear gradient separations. The study was applied to the screening of ß-adrenoceptor antagonists in urine samples, using mobile phases prepared with sodium dodecyl sulphate and 1-propanol. Predicted chromatograms were highly accurate in all situations, although suffered of baseline problems and minor shifts for peaks eluting close to a steep gradient segment. Two columns (C18 and C8) were investigated, with the C8 column being preferable owing to the smaller amount of adsorbed surfactant.
Subject(s)
Chemistry Techniques, Analytical/methods , Chromatography, Liquid , Micelles , Solvents/chemistry , 1-Propanol/chemistry , Adrenergic beta-Antagonists/analysis , Adrenergic beta-Antagonists/urine , Adsorption , Humans , Indicators and Reagents , Sodium Dodecyl Sulfate/chemistry , Surface-Active Agents/chemistryABSTRACT
The development of methacrylate-based monolithic columns was studied for the separation of pharmaceutical hydrophilic compounds in nano-liquid chromatography. The selected polymerisation mixture consisted of 7.5% hexyl methacrylate, 4.5% methacrylic acid and 18.0% ethylene dimethacrylate (w/w), in a binary porogenic solvent (35:35 w/w 1-propanol/1,4-butanediol). The polymer synthesised with this mixture has a good permeability, not excessive back-pressure, and reasonable retention times for polar and non-polar solutes. Monolithic columns (12â¯cm total capillary length, 100⯵m i.d.), prepared with this mixture, were able to produce hydrogen bonding and electrostatic interactions, giving rise to promising separations. To evaluate the chromatographic system, alkylbenzenes (neutral and hydrophobic compounds) and sulphonamides (hydrophilic drugs) were assayed. To optimise the chromatographic mobile phase in isocratic elution and characterise the retention mechanism for a mixture of eight sulphonamides, the performance of several mathematic models was checked in the description of retention. The behaviour of the monolithic capillary column was compared, in terms of selectivity and peak shape, to that obtained with a C18 column (9â¯cmâ¯×â¯4.6â¯mm i.d., 5⯵m particle size) using a conventional HPLC equipment. The results revealed substantial differences in the interactions established for sulphonamides between the monolithic and C18 columns.
ABSTRACT
The advantages and disadvantages of the use of isocratic experimental designs including transient increments of organic solvent (i.e., pulses) in the mobile phase(s) of lowest elution strength are explored with modelling purposes. For retained solutes, this type of mixed design offers similar or better predictive capability than gradient designs, shorter measurement time than pure isocratic designs, and retention model parameters that agree with those derived from pure isocratic experiments, with similar uncertainties. The predicted retention times are comparable to those offered by models adjusted from pure isocratic designs, and the solvent waste is appreciably lower. Under a practical standpoint, mixed designs including pulse(s) can be easily constructed by replacing the slowest isocratic runs with runs containing a pulse of short duration at an intermediate time. This allows the elution of the fastest solutes with appreciable retention in the initial sector of the elution program, previous to the pulse, and the elution of the slow solutes after the pulse, also in acceptable times. The fitting of the retention data obtained with pulses is simpler compared to gradient elution, and involves solving the integral equation of gradient elution, simplified by the presence of isocratic sectors. Experiments involving pulses reveal the existence of discrepancies in the predictions for solutes eluting in the nearby of the pulse, offered by the fundamental equation of gradient elution when this is solved using numerical integration. The correction of such discrepancies implies the inclusion of intra-column delays, in the arrival of changes in the concentration of organic modifier in the gradient to the instantaneous position of the solute, along the whole migration.
Subject(s)
Chromatography, Liquid/methods , Models, Chemical , Solvents/chemistry , Time FactorsABSTRACT
Peak capacity (PC) is a key concept in chromatographic analysis, nowadays of great importance for characterising complex separations as a criterion to find the most promising conditions. A theoretical expression for PC estimation can be easily deduced in isocratic elution, provided that the column plate count is assumed constant for all analytes. In gradient elution, the complex dependence of peak width with the gradient program implies that an integral equation has to be solved, which is only possible in a limited number of situations. In 2005, Uwe Neue developed a comprehensive theory for the calculation of PC in gradient elution, which is only valid for certain situations: single linear gradients, absence of delays and extra-column effects, Gaussian peaks and constant plate count. Going beyond these limitations implies resolving algebraic expressions that unfortunately cannot be integrated. In this work, PC is predicted for multiple situations based on peak simulation. The approach is more general and can be applied for situations out of the scope of the Neue outline, such as complex multi-linear gradients, including asymmetrical peaks. The plots of PC versus retention time of the last eluted solute give rise to Pareto fronts, and can be useful for the probabilistic enhancement of peak resolution in situations where complex multi-analyte samples are processed.
Subject(s)
Chromatography/methods , Models, Chemical , Computer SimulationABSTRACT
The data processing step of complex signals in high-performance liquid chromatography may constitute a bottleneck to obtain significant information from chromatograms. Data pre-processing should be preferably done with little (or no) user supervision, for a maximal benefit and highest speed. In this work, a tool for the configuration of a state-of-the-art baseline subtraction algorithm, called BEADS (Baseline Estimation And Denoising using Sparsity) is developed and verified. A quality criterion based on the measurement of the autocorrelation level was designed to select the most suitable working parameters to obtain the best baseline. The use of a log transformation of the signal attenuated artifacts associated to a large disparity in signal size between sample constituents. Conventional BEADS makes use of trial-and-error strategies to set up the working parameters, which makes the process slow and inconsistent. This constitutes a major drawback in its successful application. In contrast, the assisted BEADS simplifies the setup, shortens the processing time and makes the baseline subtraction more reliable. The assisted algorithm was tested on several complex chromatograms corresponding to extracts of medicinal herbs analysed with acetonitrile-water gradients, and a mixture of sulphonamides eluted with acetonitrile gradients in the presence of the non-ionic surfactant Brij-35 under micellar conditions.