ABSTRACT
Inflammation is the most common disease in humans. Alcohol has been part of human culture throughout history. To avoid alcohol prompting inflammation to develop into a more serious disease, it is important for human health to explore the effects of alcohol on the development of inflammation.Endogenous sulfur dioxide (SO2) is considered an important regulator of the development of inflammation and is involved in the entire development process of inflammation. Taken together, it is of great significance to explore the impact of alcohol on the development process of inflammation through changes in SO2 concentration in the inflammatory microenvironment. Herein, we report the development of a molecular tool (Nu-SO2) with rapid (5 s) response to the important inflammatory modulator sulfur dioxide (SO2) for the diagnosis of inflammation, assessment of therapeutic effects, and evaluation of the development process of alcohol-induced inflammation. The rationality of Nu-SO2 was confirmed through molecular docking calculations, density functional theory (DFT) theoretical calculations, DNA/RNA titration experiments and co-localization experiments. Furthermore, Nu-SO2 was effectively applied for specific response and highly sensitive visualization imaging of SO2 in solution, cells and mice. Importantly, Nu-SO2 was successfully used to diagnose lipopolysaccharide-induced inflammation in cells and mice and evaluate the efficacy of dexamethasone in treating inflammation. More significantly, based on the excellent performance of Nu-SO2 in dynamically reporting the further development of inflammation in mice triggered by alcohol, we successfully elucidated the "anti-inflammatory and pro-inflammatory" trend in the development of inflammation caused by alcohol stimulation. Thus, this work not only advances the research on the relationship between alcohol, inflammation and SO2, but also provides a new non-invasive assessment method for the development mechanism of inflammation induced by external stimuli and the precise diagnosis and treatment of drug efficacy evaluation.