Best Host Age of Anastrepha obliqua (Diptera: Tephritidae) for Multiplication of Four Native Parasitoids from the Americas.

The success of the mass rearing of parasitoids is directly related to host quality, and it requires selecting the best biological host age to ensure the optimal performance of the parasitoids released into the field. The larval development of the parasitoids Utetes anastrephae (Viereck) (Hymenoptera: Braconidae) and Odontosema anastrephae Borgmeier (Hymenoptera: Eucoilidae) and the pupal development of the parasitoids Coptera haywardi (Ogloblin) (Hymenoptera: Diapriidae) and Dirhinus sp. (Hymenoptera: Chalcididae) on the native host Anastrepha obliqua (Macquart) (Diptera: Tephritidae) in different larvae and pupae ages were investigated under laboratory conditions. Not all parasitoid species developed with the same efficiency in immature individuals of A. obliqua; U. anastrephae and C. haywardi showed the higher parasitism rates. The emergence and parasitism of U. anastrephae were equal using larvae from 5 to 8 d, while C. haywardi reared in 1- to 8-d-old pupae showed higher averages of parasitism. These results suggest that native parasitoids can be used to strengthen the implementation of biological control projects against A. obliqua, a pest of economic importance in South America.

Strategies for Establishing a Rearing Technique for the Fruit Fly Parasitoid: Doryctobracon brasiliensis (Hymenoptera: Braconidae).

Anastrepha fraterculus (Wiedemann) (Diptera: Tephritidae) is the most important pest in South American orchards. When control measures are not adopted, this pest can cause losses of up to 100%. Doryctobracon brasiliensis (Szépligeti) (Hymenoptera: Braconidae) is a larval-pupal endoparasitoid that can be used as a native biological control agent against A. fraterculus. This study aimed to develop a rearing technique for D. brasiliensis in larvae of A. fraterculus. Trials were carried out to: 1) determine the optimal instar for parasitism, 2) define the exposure time of larvae to parasitoids, 3) determine the density requirements of A. fraterculus larvae offered to each parasitoid, and 4) evaluate the effect of diet on adults of D. brasiliensis. In all trials, we evaluated the number of offspring, parasitism rate, and sex ratio. Moreover, in the experiment to investigate the effects of diet, we determined the longevity of males and females. In both choice and nonchoice parasitism tests, the parasitoids preferred third-instar larvae of A. fraterculus over second- and first-instar larvae. An exposure time of 12 h of A. fraterculus larvae produced larger numbers of parasitoids and higher parasitism rates. The density of 15 larvae of A. fraterculus to each female of D. brasiliensis produced a larger number of offspring. A supply of honey solution (20 and 50%) to the parasitoids yielded the highest number offspring and resulted in greater longevity. Our findings can be used to support the development of a mass rearing protocol for D. brasiliensis.

Effect of temperature on immatures of Stiretrus decastigmus (Hemiptera: Pentatomidae) / Efeito da temperatura sobre imaturos de Stiretrus decastigmus (Hemiptera: Pentatomidae)

Abstract Stiretrus decastigmus (Herrich-Schaeffer) (Hemiptera: Pentatomidae) is an important predator of the insect pest Microtheca ochroloma Stal (Coleoptera: Chrysomelidae). The present study investigated the pre-imaginal development of S. decastigmus at different temperatures. The temperatures were: 20, 25, and 30 °C, with a relative humidity of 70 ± 10% and a photofase of 12 h, and the nymphs were fed larvae of M. ochroloma. We evaluated the duration and viability of the egg and nymphal stages, the duration of each instar, and the predation potential. The incubation time decreased with increasing temperature, and the viability was highest at 25 °C. The duration of the nymphal stage was inversely proportional to the temperature, ranging from 18 days at 30 °C to 40.6 days at 20 °C. The highest S. decastigmus predation rates were found at 20 °C (90.4 larvae) and 30 °C (72.5 larvae). S. decastigmus showed the highest viability and lowest consumption of larvae of M. ochroloma at 25 °C.(AU)

Resumo Stiretrus decastigmus (Herrich-Schaeffer) (Hemiptera: Pentatomidae) é um importante predador do inseto-praga Microtheca ochroloma Stal (Coleoptera: Chrysomelidae). O objetivo deste trabalho foi estudar o desenvolvimento pré-imaginal de S. decastigmus em diferentes temperaturas. Foram utilizadas as temperaturas de 20, 25, e 30 °C, umidade relativa do ar de 70 ± 10% e fotofase de 12 h, sendo as ninfas alimentadas com larvas de M. ochroloma. Foram avaliados a duração e viabilidade dos estágios de ovo e ninfa, a duração de cada instar e o potencial de predação. O período de incubação diminuiu com o aumento da temperatura e apresentou maior viabilidade a 25 °C. A duração do estágio ninfal foi inversamente proporcional a temperatura com 18 dias a 30 °C e 40,6 dias a 20 °C. A maior taxa de predação de S. decastigmus foi encontrada a 20 °C (90,4 larvas) e 30 °C (72,5 larvas). S. decastigmus teve maior viabilidade e menor consumo de larvas de M. ochroloma a 25 °C.(AU)

Effect of temperature on immatures of Stiretrus decastigmus (Hemiptera: Pentatomidae) / Efeito da temperatura sobre imaturos de Stiretrus decastigmus (Hemiptera: Pentatomidae)

Abstract Stiretrus decastigmus (Herrich-Schaeffer) (Hemiptera: Pentatomidae) is an important predator of the insect pest Microtheca ochroloma Stal (Coleoptera: Chrysomelidae). The present study investigated the pre-imaginal development of S. decastigmus at different temperatures. The temperatures were: 20, 25, and 30 °C, with a relative humidity of 70 ± 10% and a photofase of 12 h, and the nymphs were fed larvae of M. ochroloma. We evaluated the duration and viability of the egg and nymphal stages, the duration of each instar, and the predation potential. The incubation time decreased with increasing temperature, and the viability was highest at 25 °C. The duration of the nymphal stage was inversely proportional to the temperature, ranging from 18 days at 30 °C to 40.6 days at 20 °C. The highest S. decastigmus predation rates were found at 20 °C (90.4 larvae) and 30 °C (72.5 larvae). S. decastigmus showed the highest viability and lowest consumption of larvae of M. ochroloma at 25 °C.

Resumo Stiretrus decastigmus (Herrich-Schaeffer) (Hemiptera: Pentatomidae) é um importante predador do inseto-praga Microtheca ochroloma Stal (Coleoptera: Chrysomelidae). O objetivo deste trabalho foi estudar o desenvolvimento pré-imaginal de S. decastigmus em diferentes temperaturas. Foram utilizadas as temperaturas de 20, 25, e 30 °C, umidade relativa do ar de 70 ± 10% e fotofase de 12 h, sendo as ninfas alimentadas com larvas de M. ochroloma. Foram avaliados a duração e viabilidade dos estágios de ovo e ninfa, a duração de cada instar e o potencial de predação. O período de incubação diminuiu com o aumento da temperatura e apresentou maior viabilidade a 25 °C. A duração do estágio ninfal foi inversamente proporcional a temperatura com 18 dias a 30 °C e 40,6 dias a 20 °C. A maior taxa de predação de S. decastigmus foi encontrada a 20 °C (90,4 larvas) e 30 °C (72,5 larvas). S. decastigmus teve maior viabilidade e menor consumo de larvas de M. ochroloma a 25 °C.

Effect of temperature on immatures of Stiretrus decastigmus (Hemiptera: Pentatomidae).

Stiretrus decastigmus (Herrich-Schaeffer) (Hemiptera: Pentatomidae) is an important predator of the insect pest Microtheca ochroloma Stal (Coleoptera: Chrysomelidae). The present study investigated the pre-imaginal development of S. decastigmus at different temperatures. The temperatures were: 20, 25, and 30 °C, with a relative humidity of 70 ± 10% and a photofase of 12 h, and the nymphs were fed larvae of M. ochroloma. We evaluated the duration and viability of the egg and nymphal stages, the duration of each instar, and the predation potential. The incubation time decreased with increasing temperature, and the viability was highest at 25 °C. The duration of the nymphal stage was inversely proportional to the temperature, ranging from 18 days at 30 °C to 40.6 days at 20 °C. The highest S. decastigmus predation rates were found at 20 °C (90.4 larvae) and 30 °C (72.5 larvae). S. decastigmus showed the highest viability and lowest consumption of larvae of M. ochroloma at 25 °C.

Effect of temperature on immatures of Stiretrus decastigmus (Hemiptera: Pentatomidae)

Abstract Stiretrus decastigmus (Herrich-Schaeffer) (Hemiptera: Pentatomidae) is an important predator of the insect pest Microtheca ochroloma Stal (Coleoptera: Chrysomelidae). The present study investigated the pre-imaginal development of S. decastigmus at different temperatures. The temperatures were: 20, 25, and 30 °C, with a relative humidity of 70 ± 10% and a photofase of 12 h, and the nymphs were fed larvae of M. ochroloma. We evaluated the duration and viability of the egg and nymphal stages, the duration of each instar, and the predation potential. The incubation time decreased with increasing temperature, and the viability was highest at 25 °C. The duration of the nymphal stage was inversely proportional to the temperature, ranging from 18 days at 30 °C to 40.6 days at 20 °C. The highest S. decastigmus predation rates were found at 20 °C (90.4 larvae) and 30 °C (72.5 larvae). S. decastigmus showed the highest viability and lowest consumption of larvae of M. ochroloma at 25 °C.

Resumo Stiretrus decastigmus (Herrich-Schaeffer) (Hemiptera: Pentatomidae) é um importante predador do inseto-praga Microtheca ochroloma Stal (Coleoptera: Chrysomelidae). O objetivo deste trabalho foi estudar o desenvolvimento pré-imaginal de S. decastigmus em diferentes temperaturas. Foram utilizadas as temperaturas de 20, 25, e 30 °C, umidade relativa do ar de 70 ± 10% e fotofase de 12 h, sendo as ninfas alimentadas com larvas de M. ochroloma. Foram avaliados a duração e viabilidade dos estágios de ovo e ninfa, a duração de cada instar e o potencial de predação. O período de incubação diminuiu com o aumento da temperatura e apresentou maior viabilidade a 25 °C. A duração do estágio ninfal foi inversamente proporcional a temperatura com 18 dias a 30 °C e 40,6 dias a 20 °C. A maior taxa de predação de S. decastigmus foi encontrada a 20 °C (90,4 larvas) e 30 °C (72,5 larvas). S. decastigmus teve maior viabilidade e menor consumo de larvas de M. ochroloma a 25 °C.

Occurrence of Stiretrus decastigmus (Herrich-Schaeffer) (Hemiptera: Pentatomidae) preying on Microtheca semilaevis Stal (Coleoptera: Chrysomelidae).

Microtheca semilaevis Stal is a phythophagous insect associated to Brassicaceae, and frequently occurs with other species of beetles, such as Microtheca ochroloma Stal. Despite its importance to organic farmers, there is no information in the Brazilian literature about its natural enemies. Stiretrus decastigmus (Herrich-Schaeffer) was first found and here reported preying an adult of M. semilaevis in Chinese cabbage in Santa Maria, RS, Brazil (29°43'28″S, 53°43'19″W).