[Hydrogen sulfide balneotherapy in comprehensive sanatorium-resort treatment of post-burn scars in children]. / Serovodorodnaya bal'neoterapiya v kompleksnom sanatorno-kurortnom lechenii posleozhogovykh rubtsov u detei.

Thermal lesions in children leave behind cicatricial contractions, contractures, deformations of the wrists, feet, face. Sanatorium-resort treatment using balneotherapy is an integral part of rehabilitation measures in such patients. OBJECTIVE: To analyze the results of hydrogen sulfide balneotherapy in children with consequences of thermal injury. MATERIAL AND METHODS: A single-center observational retrospective non-controlled study was carried out, in which sanatorium-resort treatment concerning post-burn scars in 812 children aged 5-17 years was analyzed. Hydrogen sulfide balneotherapy was prescribed to patients depending on the age in mild (5-6 years) or moderate-to-high (7-17 years) exposure modes. The imported hydrogen sulfide mineral water from the T-2000 well of the Matsesta field with the H2S total concentration of 410-420 mg/l was used for treatment. The applications were performed to children alternate days, 8 procedures of balneotherapy per course. RESULTS: Lightening of the affected areas of the skin, reduction of the sensation of contraction and tension of the scars, which became softer, more elastic and more mobile with regard to the subjacent tissues have been noted in patients after the course of balneotherapy. The head mobility increased after applications in the presence of scars. The large joints' range of motion grew up. In addition, an increase in the mobility of the fingers of wrists and feet, a decrease in the stiffness of movements, increase or recovery of the affected skin's tactile sensitivity have been observed. Children well tolerated procedures, adverse events were seen in 0.7% of cases in the form of mild reactions at the beginning of the applications' course, namely of balneological (0.6%) and toxico-allergic (0.1%) nature. CONCLUSION: Hydrogen sulfide balneotherapy in combination with rehabilitation exercises and other sanatorium-resort factors is an effective mean of post-burn scars correction in children.

In vitro study of the role of lenticular epithelial calcium channels and aquaporins in the development of cataract.

The role of calcium and water channels (aquaporins) in the pathogenesis of cataract was studied in vitro. Aquaporin blockade caused opacity of the lens sooner than changes in calcium ion concentration in culture medium.

Effect of low-intensity laser radiation on peripheral blood eosinophils.

The study was performed on peripheral blood eosinophils isolated on a Ficoll-Verografin continuous-density gradient. Low-intensity laser radiation (lambda=0.89 mu, 25-30 mW power, 8 min exposure) induced degranulation of peripheral blood eosinophils. The effect of low-intensity laser radiation was realized via activation of calcium channels.

[Pathophysiologic mechanisms of tissue revascularization using high intensity laser exposure]. / Patofiziologicheskii mekhanizm revaskuliarizatsii tkanei s pomoshch'iu vozdeistviia vysokointensivnogo lazernogo izlucheniia.

Satellite cellular populations (mast cells, blood platelets and fibroblasts) were found to be the promoters in forming the new vessels. When irradiated by laser, the mentioned cells act as the main source for the angiogenic growth factors and matrix metalloproteinases, which comprehensively stimulate the proliferation and migration of endothelial and smooth-muscle vascular cells. Neo-angiogenesis, in response to the laser impact, is a stereotype, adaptive reaction of tissues to laser damage irrespective of a tissue type.

Expression of vascular endothelial growth factor during neoangiogenesis stimulated by exposure to high-intensity laser radiation.

Expression of vascular endothelial growth factor was studied during neoangiogenesis stimulated by exposure of ischemic tissues in the limb muscles, liver, and myocardium and normal myocardial tissue to high-intensity laser radiation. Changes in expression were related to cell reactions during the inflammatory and reparative process and did not differ in the studied tissues.

[Mechanism of laser radiation action at tissue and cellular levels]. / Mekhanizm deistviia lazernogo oblucheniia na tkanevom i kletochnom urovniakh.

The paper describes the action of low-intensity laser radiation (LILR) and high-intensity one (HILR) on biological tissues. LILR produces its action on cells by changing the membranous formations-receptors and ion channels. At cardiac surgery, HILR stimulates uncontractile myocardial elements, by inducing the secretion of biologically active substances and the activation of enzyme systems that ensure the implementation of neoangiogenesis in the ischemic myocardium.

[The effect of heparin on the process of colony formation from bone marrow mononuclears in a diffusion chamber]. / Vliianie geparina na protsess obrazovaniia kolonii iz kostnomozgovykh mononuklearov v usloviiakh diffuzionnoi kamery.

In the experiments on Wistar rats to whom heparin was injected intravenously in the tail vein in doses of 50, 100 and 250 U, there were studied the colony-forming ability of the hemopoietic cells, the precursors of the bone marrow, and the colony-stimulating activity of the plasma of the heparinized animals 1, 3, 6, 12 and 24 hours after the drug administration. Heparin was found to increase the proliferative activity of the committed myelopoietic cells-precursors on a diffusion microchamber (GM-CFUdc) stimulating the mononuclears and elevating the level of the colony-stimulating activity of the plasma of the experimental animals.

[Bone marrow erythroblast islets in burns]. / Eritroblasticheskie ostrovki kostnogo mozga pri ozhoge.

The character of changes of rat bone marrow (BM) erythroblastic islets (EI) was studied in different periods after III degree burn of 15% body surface. Marked differences in the qualitative EI composition were revealed in the cortical generative zone and the central maturation zone of control animals. Proliferative EI forms predominated in the generative zone and involute EI in the maturation zone. The absolute number of EI in BM reduced in the early period of burn anaemia, probably due to proteolysis mainly through a decrease of involute EI in the maturation zone. The number of EI in BM reached minimal level in the late period of burn anaemia. Inhibition of erythropoiesis in the EI was manifested by a decrease in the content of proliferating forms of EI predominantly in the BM generative zone, probably due to slower involvement of erythroid CFU cells in erythropoiesis and inhibition of specific functions of EI macrophages.

[The effect of exogenous heparin on the processes of colony formation from myeloid precursors and the formation of erythroblastic islets]. / Vliianie ékzogennogo geparina na protsessy kolonieobrazovaniia iz mieloidnykh predshestvennikov i obrazovanie éritroblasticheskikh ostrovkov.

Experiments were conducted on Wistar rats to study the comparative effect of exogenous administration of heparin (250 U) on the colony forming ability of committed myeloid precursors and the formation of erythroblast islets (EI). The results were recorded for 5 days. On the basis of the obtained data a primary selective effect of heparin on the granulocytic-macrophagal colony forming units in the diffusion chamber was revealed. The process of EI formation is affected only in the late periods (3rd-5th day) against the background of sharply stimulated myelopoiesis. A significant reduction of the number of EI is noted in this period.

[The quantitative and qualitative characteristics of the reaction of committed bone marrow cell precursors in rats to burn trauma]. / Kolichestvennaia i kachestvennaia kharakteristika reaktsii kommitirovannykh kletok-predshestvennikov kostnogo mozga krys na ozhogovuiu travmu.

The article describes the dynamics of colony-forming units in diffusion chamber of tubular bone marrow compartments 1 and 7 days after a burn. It was found that under normal conditions the activity of the axial part of the bone marrow is twice that of the cortical part. After a burn the inductive capacity of the cortical part becomes equal to that of the axial part. A wider qualitative spectrum of colonies occurs due to the effect of short-distance humoral factors (eosinophils, mast cells).

[Lipoxin B, an enhancing factor of spontaneous platelet aggregation in whole blood]. / Lipoksin B--faktor usileniia spontannoi agregatsii trombotsitov v tsel'noi krovi.

The arachidonic acid metabolites, lipoxin B (5,14,15-trihydroxy-6,8,10,12-eicosatetraenoate) and 5,15-dihydroxy-eicosatetraenoate were shown to possess a marked effect on platelet aggregation and procoagulant activity. Lipoxin B increases and 5,15-dihydroxy-eicosatetraenoate decreases spontaneous platelet aggregation and procoagulant activity when added at various concentrations. The drugs tested were found to have no direct action on blood coagulation.

[Direct atomic absorption determination of the 3d elements (Fe, Cu, Mn, Co, Ni) and zinc in biological material]. / Priamoe atomno-absorbtsionnoe opredelenie 3d-élementov (Fe, Cu, Mn, Co, Ni) i Zn v biologicheskom materiale.

The authors' findings evidence the possibility of the blood serum, liver, spleen, and bone marrow Fe, Cu, Mn, Co, Ni, and Zn measurements by the direct atomic absorption method without their separation and extraction. The technique is simple, meets the requirements to physicochemical analyses, and may be recommended for investigations in theoretical and practical medicine. The method is not devoid of a number of drawbacks, that limit the range of its applications; this concerns, among other things, Co measurements. The studies have demonstrated significant differences in the levels of trace elements in the tested biological material, which is explained by the species and organic characteristics of their metabolism.

[Effects of lipoxin B on colony-forming capacity of human peripheral blood mononuclear cells in diffusion chambers]. / Vliianie lipoksina B na kolonieobrazuiushchuiu sposobnost' mononuklearov perifericheskoi krovi cheloveka v usloviiakh diffuzionnoi kamery.

The lipoxin B (5, 14, 15-trihydroxy-6, 8, 10, 12-eicosatetraenoate) obtained by the enzymatic peroxidation of 5, 15-dihydroxy-6, 8, 11, 13-eicosatetraenoate (5, 15-DHETE) with the homogenous rabbit reticulocytes lipoxygenase and purified by HPLC stimulates the proliferation and differentiation of granulocyte-monocyte colony formation units (CFU-GMdc) of human peripheral blood at 3.6 X 10(-9) 3.6 X 10(-8)M in diffusion chamber placed in mouse abdominal cavity. The lipoxin B precursors namely 5,15-DHETE and arachidonic acid had stimulating influence at about 10(-6) and 10(-3)M respectively. The formation of polynuclear cells under the action of the studied lipoxygenase metabolites of the arachidonic acid was shown.