Occurrence of antibiotics in Lettuce (Lactuca sativa L.) and Radish (Raphanus sativus L.) following organic soil fertilisation under plot-scale conditions: Crop and human health implications.

Recent studies have demonstrated the crop uptake of antibiotics (ABs) from soils treated with AB-carrying fertilisers. However, there is a lack of plot-scale studies linking their effects at the agronomic and metabolomic/transcriptomic level to their impact on human health. This paper assesses the plant uptake of 23 ABs following two productive cycles of lettuce and radish cropped with sewage sludge, pig slurry, the organic fraction of municipal solid waste, or chemical fertilisation under plot-scale conditions (32 plots spanning 3-10 m2 each). AB uptake by plants depended on both the vegetable and the AB class and was higher in radish than in lettuce edible parts. Levels ranged from undetectable to up to 76 ng/g (fresh weight). Repetitive organic fertilisation resulted in an increase in the concentration of ABs in lettuce leaves, but not in radish roots. Significant metabolomic and transcriptomic changes were observed following soil fertilisation. Nevertheless, a human health risk assessment indicates that the occurrence of ABs in lettuce or radish edible parts does not pose any risk. To our knowledge, this is the first holistic plot-scale study demonstrating that the use of organic fertilisers containing ABs is safe for crop security and human health.

What Is Going on With the Genus Dermacentor? Hybridizations, Introgressions, Oh My!

In the 1930s, R. A. Cooley noted that Dermacentor occidentalis (Acarina: Ixodidae) and Dermacentor andersoni were closely related and could hybridize. Decades later, James Oliver discovered that crosses of Dermacentor variabilis, D. andersoni, and D. occidentalis could, on occasion, produce hybrids. A recent molecular analysis (both mtDNA and nDNA) in our laboratory revealed that certain specimens of Dermacentor andersoni nested with Dermacentor parumapertus. Does this close relationship, along with the mito-nuclear discordance we have observed, mean D. andersoni and D. parumapertus are a single species? By contemporary taxonomic criteria, this seems improbable based on their distinctly different morphologies, host associations, and ecologies. This paper explores ideas related to mito-nuclear discordance, hybridization, and introgression (primarily) not only in these two species but also other members of the genus Dermacentor. Both D. andersoni and D. parumapertus can be found on the same hosts and have sympatric distributions, so introgression of genetic material by occasional cross-mating between these two species is possible. Further, the difficulty in applying specific species concepts in ticks has been recently pointed out and a unified agreement on an integrative species concepts could clearly be useful in this situation. With the discovery of D. parumapertus as a potential vector of Rickettsia parkeri and the historically recognized role of D. andersoni in transmission of Rickettsia rickettsii, understanding the specific status of each lineage of these species (and others in the genus) is extremely important from a public health perspective.

Molecular Phylogeny of Dermacentor parumapertus (Acari: Ixodidae) from Two Locations Within Its Geographical Range.

Dermacentor parumapertus Neumann (Acari: Ixodidae) is a rather obscure tick found on jackrabbits in the western United States and parts of Canada and Mexico. Specimens from the northern part of their range are consistently different morphologically from ones found in southern and eastern parts of their range (particularly west Texas), leading some researchers to declare the southern form a variety or subspecies. This study examined field-collected adult D. parumapertus from two main locations-Utah and Texas-within its geographic distribution to ascertain the degree of genetic divergence in the two populations based upon both nuclear and mitochondrial DNA sequences. In total, 30 D. parumapertus were analyzed by PCR using both mtDNA and nDNA genes, and one D. nitens was included for comparison. Trees were constructed for all mtDNA genes individually, as well as after concatenating mtDNA (COI, COII, 12S) and nDNA (2 ITS2 primers), respectively. All constructed trees were exported to FigTree v1.4.3 and TreeGraph v2.14.1-771 beta for visualization. The majority of the Utah and Texas populations of D. parumapertus separated molecularly in both mtDNA and nDNA trees; however, analysis with mtDNA genes showed that 3/13 (23%) of Utah tick specimens were removed molecularly from other specimens collected at the same location. Thus, there was not enough evidence to declare these two disparate and morphologically different populations as distinct and separate species.

Effect of Zirconia Pigmentation on Translucency.

PURPOSE: the aim of this study was to investigate the effect of shading on translucency and mean grain size of three Zirconia systems (Lava Frame, Ice Zirkon Translucent and Prettau Zirkon). MATERIALS AND METHODS: specimens pigmented in A1, A4 and a non-pigmented specimen was evaluated with Scanning Electron Microscopy and spectrophotometery. RESULTS: the transmittance ranged between 0.18 and 0.52, in Ice Zirkon Translucent A4 and Prettau Zircon non-pigmented, respectively. The pigmentation A1 and A4 significantly reduced the light transmission in all the systems evaluated (p⟨0.05), with the exception of Lava A1. The light transmittance of each ceramic system is different from each other in the three pigmentations, except for the Prettau Zirkon A1, which did not show a significant difference compared with Lava A1 (p⟩0.05). The mean grain size increased with pigmentation. The mean grain size ranged between 0.43 µm, for Prettau Zirkon non-pigmented, and 0.82 µm for Prettau Zirkon A4. CONCLUSION: Light transmission and mean grain size was affected by pigmentation of dental zirconia ceramics.

A Statewide Survey for Container-Breeding Mosquitoes in Mississippi.

Container-breeding mosquitoes are important in public health due to outbreaks of Zika, chikungunya, and dengue viruses. This paper documents the distribution of container-breeding mosquito species in Mississippi, with special emphasis on the genus Aedes. Five sites in each of the 82 Mississippi counties were sampled monthly between May 1 and August 31, 2016, and 50,109 mosquitoes in 14 species were collected. The most prevalent and widely distributed species found was Ae. albopictus, being found in all 82 counties, especially during July. A recent invasive, Ae. japonicus, seems to be spreading rapidly in Mississippi since first being discovered in the state in 2011. The most abundant Culex species collected were Cx. quinquefasciatus (found statewide), Cx. salinarius (almost exclusively in the southern portion of the state), and Cx. restuans (mostly central and southern Mississippi). Another relatively recent invasive species, Cx. coronator, was found in 20 counties, predominantly in the southern one-third of the state during late summer. Co-occurrence data of mosquito species found in the artificial containers were also documented and analyzed. Lastly, even though we sampled extensively in 410 sites across Mississippi, no larval Ae. aegypti were found. These data represent the first modern statewide survey of container species in Mississippi, and as such, allows for better public health readiness for emerging diseases and design of more effective vector control programs.

Controlling Bacillus cereus adherence to stainless steel with different cleaning and sanitizing procedures used in dairy plants / Controle do processo de adesão de Bacillus cereus ao aço inoxidável após diferentes procedimentos de limpeza e sanitização usados na indústria de laticínios

Bacillus cereus adherence to stainless steel used to milk contact surfaces was observed, depending on the cleaning and sanitizing procedures applied and the physicochemical properties of the surfaces. Numbers of surviving B. cereus after hygiene procedures were affected by temperature, the concentrations of both alkaline and acid washes, and the pH of the chlorine solution. The adhesion of B. cereus to the stainless steel was not thermodynamically favorable, and the adherence of this microorganism occurred in lower number, in accordance to the thermodynamic aspect of adhesion.

Foi observada a adesão de Bacillus cereus em superfície de aço inoxidável em contato com o leite, segundo o procedimento de limpeza, a sanitização e as propriedades físico-químicas da superfície. O número de B. cereus viáveis, após os procedimentos de higienização, foi afetado pela temperatura, pela concentração das soluções alcalinas e ácidas e pelo pH da solução clorada. A adesão de B. cereus em aço inoxidável não foi termodinamicamente favorável, e ocorreu pouca adesão desse microrganismo, de acordo com os aspectos termodinâmicos da adesão.

Digestibilidade da matéria seca de silagens de milho e de suplementos concentrados determinada por procedimentos in vitro / Dry matter digestibility of corn silages and concentrates determined by in vitro procedures

Compararam-se os valores de digestibilidade in vitro da matéria seca (DIVMS) de quatro silagens de milho e de seis concentrados (três proteicos e três energéticos), determinados pelo método de dois estádios realizados em tubos individuais ou em equipamento automatizado de fermentação, e avaliou-se a ocorrência de efeito associativo na digestão de alimentos destas três classes incubadas em um mesmo jarro de fermentação da incubadora in vitro, ou em jarros diferentes contendo a mesma classe de alimentos. Utilizou-se delineamento inteiramente ao acaso, em esquema fatorial 3 x 3 (procedimentos in vitro x classes de alimentos). Os valores de DIVMS determinados pelo método dos tubos foram mais próximos dos relatados na literatura e menores (P<0,02) que os obtidos pelos procedimentos automatizados. Não houve diferença (P>0,05) nos valores de DIVMS dos concentrados e das silagens de milho incubados no mesmo jarro de fermentação da incubadora ou, separadamente por alimento, em jarros diferentes.

The values of in vitro dry matter digestibility (IVDMD) of four corn silages and six concentrates (three proteic and three energetics) were determined by the two-stage technique that utilizes individual digestion tubes and by an automatic equipment of fermentation (filter bag technique). It was also evaluated the associative effect on digestion of these three food classes when incubated in the same fermentation jar of the "in vitro incubator" and when in different vessels containing the same class of foods. The experimental design was completely randomized in a 3 x 3 factorial arrangement (in vitro procedures x food classes). The IVDMD values determined in tubes were closer to those found in the literature and lower (P<0.02) than those obtained by the automatic procedure. No differences were found (P>0.05) in IVDMD of concentrates and silages when incubated together in the same fermentation jar or separated by food classes in separated jars.

A socioenvironmental composite index as a tool for identifying urban areas at risk of lymphatic filariasis.

OBJECTIVE: To describe the spatial distribution of lymphatic filariasis and its relationship with the socioenvironmental risk indicator, thus identifying priority localities for interventions in endemic urban areas. METHODS: The study area was the municipality of Jaboatão dos Guararapes, State of Pernambuco, Brazil. The data sources were a parasitological survey and the 2000 demographic census. From these data, a socioenvironmental composite risk indicator was constructed using the 484 census tracts (CT) as the analysis units, based on the score-formation technique. Census tracts with higher indicator values presented higher risk of occurrences of filariasis. RESULTS: Six thousand five hundred and seven households were surveyed and 23 673 individuals were examined, among whom 323 cases of microfilaremia were identified. The mean prevalence rate for the municipality was 1.4%. The indicator showed that 73% (237/323) of the cases of microfilaremia were in high-risk areas (third and fourth quartiles) with worse socioenvironmental conditions (RR = 4.86, CI = 3.09-7.73, P < 0.05). CONCLUSIONS: The socioenvironmental composite risk indicator demonstrated sensitivity, since it was able to identify the localities with greater occurrence of infection. Because it can stratify spaces by using official and available data, it constitutes an important tool for use in the worldwide program for eliminating lymphatic filariasis.

The socio-demographic, environmental and reservoir factors associated with leptospirosis in an urban area of north-eastern Brazil.

In an ecological study based on the 18 microregions that form the city of Recife, the capital of the Brazilian state of Pernambuco, associations between socio-demographic, environmental and reservoir factors and the incidence of leptospirosis in the city were investigated. Incidence over a 5-year period (2001-2005) and 14 variables were analysed, using central trend and dispersion measurements, Pearson's correlation and multiple linear regression. Variables relating to education, income, housing type, sewage system, rubbish collection and hydrographic factors were found to be significantly correlated with leptospirosis incidence (P<0.05 for each). Just two variables - the proportion of heads of households with incomes less than or equal to the legal minimum (U.S.$83.55/month), and the proportion of households from which rubbish was dumped in skips, lakes, rivers or the sea or on vacant land - explained 60% (P=0.017) of the differences in disease risk observed between the various areas of the city.

[Life-saving total gastrectomy in abdominal sepsis after bariatric surgery of gastric sleeve]. / Degastrectomía total salvadora de vida en sepsis abdominal post cirugía bariatrica de manga gástrica.

Three obese patients underwent sleeve gastrectomy. They developed gastric dehiscence with generalized peritonitis, sepsis and shock, being operated as total gastrectomy of the remanent stomach with Roux in Y esophago-jejunostomy reconstruction as a last chance of life saving procedure.

Degastrectomía total salvadora de vida en sepsis abdominal post cirugía bariatrica de manga gástrica / Life-saving total gastrectomy in abdominal sepsis after bariatric surgery of gastric sleeve

Presentamos la experiencia de tres pacientes obesos que fueron sometidos a tratamiento quirúrgico de manga gástrica y que desarrollaron dehiscencia de sutura gástrica con peritonitis generalizada, sepsis y shock por lo que fueron sometidos a degastrectomía total (resección del remanente gástrico) y reconstrucción inmediata de esófago yeyuno anastomosis en Y de Roux como última medida de tratamiento salvador de vida.

Three obese patients underwent sleeve gastrectomy. They developed gastric dehiscence with generalized peritonitis, sepsis and shock, being operated as total gastrectomy of the remanent stomach with Roux in Y esophago-jejunostomy reconstruction as a lastchance of life saving procedure.

Heterogeneous DNA binding modes of berenil.

Isothermal titration calorimetry (ITC) profiles of berenil bound to different DNAs show that, despite the strong preference of berenil for AT-rich regions in DNA, it can bind to other DNA sequences significantly. The ITC results were used to quantify the binding of berenil, and the thermodynamic profiles were obtained using natural DNAs as well as synthetic polynucleotides. ITC binding isotherms cannot be simply described when a single set of identical binding sites is considered, except for poly[d(A-T)2]. Ultraviolet melting of DNA and differential scanning calorimetry were also used to quantify several aspects of the binding of berenil to salmon testes DNA. We present evidence for secondary binding sites for berenil in DNA, corresponding to G+C rich sites. Berenil binding to poly[d(G-C)2] is also observed. Circular dichroism experiments showed that binding to GC-rich sites involves drug intercalation. Using a molecular modeling approach we demonstrate that intercalation of berenil into CpG steps is sterically feasible.

Analysis of the effects of daunorubicin and WP631 on transcription.

The proficiency with which anthracyclines and other DNA-binding drugs target certain sequences in eukaryotic promoters offers a potential approach to interfere with the mechanisms that regulate gene expression in tumor cells. An in vitro transcription assay has been used to compare the ability of the bisintercalating anthracycline WP631 and the monointercalating anthracycline daunorubicin in terms of their ability to inhibit initiation of transcription of the adenovirus major late promoter linked to a G-less transcribed DNA template. Both drugs inhibit basal transcription by RNA polymerase II. However, WP631 is approximately 15 times more efficient at inhibiting transcription initiation from an adenovirus promoter containing an upstream Sp1-protein binding site. The differences in the ability of each drug to inhibit transcription initiation appear to be related to the competition between Sp1 and the anthracyclines for binding to the same site. To see whether WP631's strong effect on transcription can also be observed in cells, we compared the effects of WP631 and other anthracyclines on the transcription of the c-myc gene, which promoter contains Sp1 binding sites. The resulting data suggest that WP631 might circumvent some kinds of tumor resistance at rather low drug concentrations, inhibit c-myc expression in some cell lines, and exert its antitumoral effect by inducing apoptosis.

Efeito da temperatura ambiente e da umidade relativa do ar sobre a freqüência de ingestäo de alimentos e de água e de ruminaçäo em vacas da raça Holandesa / Effect of air temperature and humidity on frequency of feeding, water ingestion and rumination in Holstein cows

Estudaram-se a ingestäo de alimentos e de água e a ruminaçäo em vacas primíparas e multíparas da raça Holandesa, com alta produçäo de leite, criadas em sistema de confinamento do tipo free stall, em diferentes combinaçöes de temperatura ambiente e umidade relativa do ar. Foram realizadas três coletas de dados nos meses de veräo e três nos de inverno, todas elas com duraçäo de 24 horas. As atividades de alimentaçäo e ruminaçäo foram monitoradas em intervalos de 15 minutos e a de ingestäo de água, continuamente ao longo das 24 horas. A temperatura ambiente e a umidade relativa do ar foram observadas em intervalos de uma hora. O binômio temperatura ambiente/umidade do ar alteraram significativamente (P<0,05) o padräo de alimentaçäo de vacas e novilhas nas coletas de veräo, período de alimentaçäo esperado de 6h-18h, para o intervalo de 18h-24h. A ruminaçäo foi afetada principalmente pelo padräo diário de alimentaçäo. O padräo de ingestäo de água, da mesma forma, variou significativamente (P<0,05) em funçäo do comportamento de alimentaçäo e das condiçöes ambientais (temperatura ambiente e umidade do ar), marcadamente no lote de vacas

Efeito da temperatura ambiente e da umidade relativa do ar sobre a freqüência de ingestão de alimentos e de água e de ruminação em vacas da raça Holandesa

The feeding behaviour, rumination and water consumption of primiparous and multiparous Holstein cows, housed in a free-stall system were studied, in response to different combinations of temperature and relative air humidity. Data were collected during three days (24 hours) in the summer and winter months. Feeding behaviour and rumination were monitored in each 15 minute intervals, and the water ingestion, continuously all day long. Environmental temperature and relative humidity were also monitored in each hour interval. During summer time, the environmental temperature and relative humidity significantly affected (P 0.05) feeding behaviour of the cows with an increasing time of feeding intervals, from 6am - 6pm to 6pm - 12pm. Daily pattern of rumination was defined by the daily pattern of feeding. The water ingestion varied significantly (P 0.05) as a function of the feeding behavior due to environmental temperature and air humidity, mainly in the cow group.

Estudaram-se a ingestão de alimentos e de água e a ruminação em vacas primíparas e multíparas da raça Holandesa, com alta produção de leite, criadas em sistema de confinamento do tipo free stall, em diferentes combinações de temperatura ambiente e umidade relativa do ar. Foram realizadas três coletas de dados nos meses de verão e três nos de inverno, todas elas com duração de 24 horas. As atividades de alimentação e ruminação foram monitoradas em intervalos de 15 minutos e a de ingestão de água, continuamente ao longo das 24 horas. A temperatura ambiente e a umidade relativa do ar foram observadas em intervalos de uma hora. O binômio temperatura ambiente/umidade do ar alteraram significativamente (P 0,05) o padrão de alimentação de vacas e novilhas nas coletas de verão, período de alimentação esperado de 6h-18h, para o intervalo de 18h-24h. A ruminação foi afetada principalmente pelo padrão diário de alimentação. O padrão de ingestão de água, da mesma forma, variou significativamente (P 0,05) em função do comportamento de alimentação e das condições ambientais (temperatura ambiente e umidade do ar), marcadamente no lote de vacas.

The use of socioeconomic factors in mapping tuberculosis risk areas in a city of northeastern Brazil.

In Brazil the challenge of meeting the needs of those living in deprived areas has generated discussions on replacing the existing approach to epidemiological surveillance with an integrated public health surveillance system. This new approach would supplant the traditional focus on high-risk individuals with a method for identifying high-risk populations and the areas where these persons live. Given the magnitude of the problem that tuberculosis (TB) poses for Brazil, we chose that disease as an example of how such a new, integrated public health surveillance system could be constructed. We integrated data from several sources with geographic information to create an indicator of tuberculosis risk for Olinda, a city in the Brazilian state of Pernambuco. In order to stratify the urban space in Olinda and to check for an association between the resulting TB risk gradient and the mean incidence of the disease between 1991 and 1996, we applied two different methods: 1) a "social deprivation index" and 2) principal component analysis followed by cluster analysis. Our results showed an association between social deprivation and the occurrence of TB. The results also highlighted priority groups and areas requiring intervention. We recommend follow-up that would include treating acid-fast bacilli smear-positive pulmonary TB cases, tracing of these persons' contacts, and monitoring of multidrug-resistant cases, all in coordination with local health services.

Bisanthracycline WP631 inhibits basal and Sp1-activated transcription initiation in vitro.

An in vitro transcription assay was used to compare the capacity of the bisintercalating anthracycline WP631 (which displays a remarkably high DNA-binding affinity) and the monointercalating anthracycline daunomycin to inhibit transcription initiation of the adenovirus major late promoter linked to a G-less transcribed DNA template. Both drugs inhibit basal RNA synthesis in a concentration-dependent way, and the drug concentrations required to inhibit transcription initiation are similar. However, in this study WP631 was around 15 times more efficient at inhibiting transcription initiation when used with an adenovirus promoter containing an upstream Sp1-protein binding site under experimental conditions in which the Sp1 protein acted as a transactivator in vitro. The differences in the ability of each drug to inhibit transcription initiation were related to the competition between Sp1 and the drugs for the same binding site. Concentrations of WP631 as low as 60 nM could inhibit the Sp1-activated transcription initiation in vitro. In contrast, the concentration of daunomycin required to inhibit Sp1-activated transcription by 50% was almost the same as the concentration required to inhibit basal transcription. The efficiency of WP631 at displacing Sp1 from its putative binding site was confirmed using gel retardation and footprinting assays. These results are the first unequivocal example of a direct effect of an intercalator on activated transcription initiation.