The role of advanced training in family planning and reproductive health in a low-income country; the experience of Ethiopia.

Low- and middle-income countries continue to experience high fertility rates and unsafe abortion. Increased access to safe abortion services and family planning are cost-effective ways to reduce maternal morbidity and mortality. With a vision of improving the reproductive health workforce of the country, St. Paul's Hospital Millennium Medical College, in partnership with a university in the United States (U.S.), launched the first family planning and reproductive health fellowship program in Ethiopia. As the premier program in the country, the fellowship has introduced several new initiatives and skills to the existing reproductive health care training options. This program is a stirring example of successful collaboration between a U.S. university and a college in a low- or middle-income country. We have summarized the process of establishing the fellowship program as the first experience in Ethiopia and East Africa.

WITHDRAWN: Fighting for access: Research at the National Abortion Federation's 43rd Annual Meeting.

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Examining the Potential Role of Foliar Chemistry in Imparting Potato Germplasm Tolerance to Potato Psyllid, Green Peach Aphid, and Zebra Chip Disease.

Long-term, sustainable management of zebra chip disease of potato, caused by 'Candidatus Liberibacter solanacearum' (Lso) and vectored by potato psyllids (Bactericera cockerelli Sulc [Hemiptera: Triozidae]), requires development of cultivars resistant or tolerant to infection or capable of reducing spread or both. We examined the influence that five experimental breeding clones of potato had on potato psyllids and their ability to vector Lso. The ability of these potato clones to resist aphids (green peach aphids, Myzus persicae Sulzer [Hemiptera: Aphididae]) also was examined. Due to the importance of host chemistry on plant-insect interactions, levels of primary metabolites of amino acids and sugars, as well as secondary metabolites including polyphenolics, terpenoids, and alkaloids were compared between breeding clones and a commercial cultivar. Findings for compound levels then were associated with observed changes in host susceptibility to psyllids or aphids. Psyllids oviposited less on three breeding clones than Atlantic, but no significant effects of breeding clones on psyllid feeding or choice were observed. Aphid reproduction was reduced on two clones relative to Atlantic. A05379-211 had greater sugar levels and postpsyllid amino acid levels than Atlantic. Total alkaloid and phenolic levels were greater in all breeding clones than Atlantic. Total terpenoid levels were greater in PALB03016-3 and PALB03016-6 than Atlantic, which might explain, in part, the observed resistance to psyllid oviposition and aphid reproduction. Overall, these results suggest that increased levels of certain metabolites in breeding clones could affect psyllid and aphid reproduction.

Tumor necrosis factor-inducible gene 6 protein: A novel neuroprotective factor against inflammation-induced developmental brain injury.

Inflammation is an important factor contributing to developmental brain injury in preterm infants. Although tumor necrosis factor-inducible gene 6 protein (TSG-6) has immunomodulatory effects in several inflammatory conditions of adult animals, nothing is currently known about the role of TSG-6 in the developing brain, its impact on perinatal inflammation and its therapeutic potential. The aim of the current work was 1) to characterize the developmental expression of TSG-6 in the newborn rat brain, 2) to evaluate the impact of LPS exposure on TSG-6 expression and 3) to assess the therapeutic potential of exogenous TSG-6 administration. Brain hemispheres of healthy Wistar rats (postnatal day 1-postnatal day 15 (P1-P15)) were evaluated with regard to the physiological expression of TSG-6. LPS-treated rats (0.25mg/kg LPS i.p. on P3) were analyzed for inflammation-induced changes in TSG-6 and cytokine expression. To evaluate whether exogenous recombinant human (rh)TSG-6 affects inflammation-induced brain injury, newborn Wistar rats, exposed to LPS on P3, were treated with rhTSG-6 i.p. (four repetitive doses of 2.25mg/kg every 12h, first dose 3h before LPS injection). PCR, Western blotting and multiplex ELISA were performed according to standard protocols. TSG-6 is physiologically expressed in the developing brain with a linear increase in expression from P1 to P15 at the mRNA level. At P6, regional differences in TSG-6 expression in the cortex, thalamus and striatum were detected at mRNA and protein level. Furthermore, TSG-6 gene expression was significantly increased by inflammation (induced by LPS treatment). Combined treatment with LPS and TSG-6 vs. LPS exposure alone, resulted in significant down-regulation of cleaved caspase-3, a marker of apoptosis and neuronal plasticity. In addition, several inflammatory serum markers were decreased after TSG-6 treatment. Finally, TSG-6 is physiologically expressed in the developing brain. Changes of TSG-6 expression associated with inflammation suggest a role of TSG-6 in neuroinflammation. Reduction of cleaved caspase-3 by TSG-6 treatment demonstrates the putative neuroprotective potential of exogenous TSG-6 administration in inflammation-induced developmental brain injury.

De Novo Genome Sequence of "Candidatus Liberibacter solanacearum" from a Single Potato Psyllid in California.

The draft genome sequence of "Candidatus Liberibacter solanacearum" strain RSTM from a potato psyllid (Bactericera cockerelli) in California is reported here. The RSTM strain has a genome size of 1,286,787 bp, a G+C content of 35.1%, 1,211 predicted open reading frames (ORFs), and 43 RNA genes.

Intravitreal bevacizumab (Avastin) versus triamcinolone (Volon A) for treatment of diabetic macular edema: one-year results.

PURPOSE: The objective was to compare retinal morphology and function following intravitreal injections of bevacizumab (Avastin) or triamcinolone (Volon A) in patients with early diabetic macular edema (DME). PATIENTS AND METHODS: The study was planned as a randomized, prospective, interventional clinical trial. A total of 30 diabetic patients with treatment-naïve, clinically significant macular edema were included in this study and randomized to two equal groups. One group initially received three injections of 2.5 mg bevacizumab in monthly intervals. The second group received a single injection of 8 mg triamcinolone, followed by two sham interventions. Functional and anatomic results were evaluated monthly using ETDRS vision charts and spectral-domain optical coherence tomography. According to the study protocol, retreatment after 3 months was dependent on functional and anatomic outcome in a PRN regimen. RESULTS: Baseline best corrected visual acuity (BCVA) was 0.30 logMAR and central retinal subfield thickness (CSRT) was 505 µm in the bevacizumab group and 0.32 logMAR and 490 µm CSRT in the triamcinolone group. After 3 months, BCVA improved to 0.23 logMAR (bevacizumab) and 358 µm CRST and 0.26 logMAR (triamcinolone) and 308 µm CSRT. After 12 months, BCVA further recovered in the bevacizumab group (0.18 logMAR) but slightly decreased in the triamcinolone group (0.36 logMAR). CONCLUSION: Intravitreal bevacizumab and triamcinolone are both equally effective in reducing CSRT in early DME. After 6 months, rehabilitation of vision was comparable in both treatment arms, whereas at the final follow-up at month 12, BCVA was superior in the bevacizumab than in the triamcinolone sample. This may be related to cataract development following steroid treatment, as well as to substance-specific mechanisms within the angiogenic versus the inflammatory cascade.

Prospective assessment of fetal-maternal cell transfer in miscarriage and pregnancy termination.

BACKGROUND: Fetal cells (microchimerism) are acquired by women during pregnancy. Fetal microchimerism persists decades later and includes cells with pluripotent capacity. Persistent microchimerism has the capacity for both beneficial and detrimental maternal health consequences. Both miscarriage and termination of pregnancy can result in fetal microchimerism. We sought to determine whether cellular fetal microchimerism is acquired during management of pregnancy loss and further explored factors that could influence fetal cell transfer, including viability of fetal tissue, surgical versus medical management and gestational age. METHODS: Pregnant women (n= 150 samples from 75 women) with singleton pregnancies undergoing a TOP (n= 63) or treatment for embryonic or fetal demise (miscarriage, n= 12) were enrolled. Mononuclear cells were isolated from blood samples drawn before, and 30 min after, treatment. Fetal cellular microchimerism concentrations were determined using quantitative PCR for a Y chromosome-specific sequence, expressed as genome equivalents of fetal DNA per 100 000 maternal cell equivalents (gEq/10(5)). Detection rate ratios were determined according to clinical characteristics. RESULTS: Cellular fetal microchimerism was found more often in post- compared with pretreatment samples, 24 versus 5% (P= 0.004) and at higher concentrations, 0-36 versus 0-0.7 gEq/10(5) (P< 0.001). Likelihood of microchimerism was higher in surgical than medical management, detection rate ratio 24.7 (P= 0.02). The detection rate ratio for TOP versus miscarriage was 16.7 for known male fetuses (P= 0.02). Microchimerism did not vary with gestational age. CONCLUSIONS: Significant fetal cell transfer occurs during miscarriage and TOP. Exploratory analyses support relationships between obstetric clinical factors and acquisition of fetal cellular microchimerism; however, our limited sample size precludes definitive analysis of these relationships, and confirmation is needed. In addition, the long-term persistence and potential consequences of fetal microchimerism on maternal health merit further investigation.

Prevention of neonatal oxygen-induced brain damage by reduction of intrinsic apoptosis.

Within the last decade, it became clear that oxygen contributes to the pathogenesis of neonatal brain damage, leading to neurocognitive impairment of prematurely born infants in later life. Recently, we have identified a critical role for receptor-mediated neuronal apoptosis in the immature rodent brain. However, the contribution of the intrinsic apoptotic pathway accompanied by activation of caspase-2 under hyperoxic conditions in the neonatal brain still remains elusive. Inhibition of caspases appears a promising strategy for neuroprotection. In order to assess the influence of specific caspases on the developing brain, we applied a recently developed pentapeptide-based group II caspase inhibitor (5-(2,6-difluoro-phenoxy)-3(R,S)-(2(S)-(2(S)-(3-methoxycarbonyl-2(S)-(3-methyl-2(S)-((quinoline-2-carbonyl)-amino)-butyrylamino)propionylamino)3-methylbutyrylamino)propionylamino)-4-oxo-pentanoic acid methyl ester; TRP601). Here, we report that elevated oxygen (hyperoxia) triggers a marked increase in active caspase-2 expression, resulting in an initiation of the intrinsic apoptotic pathway with upregulation of key proteins, namely, cytochrome c, apoptosis protease-activating factor-1, and the caspase-independent protein apoptosis-inducing factor, whereas BH3-interacting domain death agonist and the anti-apoptotic protein B-cell lymphoma-2 are downregulated. These results coincide with an upregulation of caspase-3 activity and marked neurodegeneration. However, single treatment with TRP601 at the beginning of hyperoxia reversed the detrimental effects in this model. Hyperoxia-mediated neurodegeneration is supported by intrinsic apoptosis, suggesting that the development of highly selective caspase inhibitors will represent a potential useful therapeutic strategy in prematurely born infants.

Experimental observation of anisotropic magnetic turbulence in a reversed field pinch plasma.

In this Letter we report an experimental study of fully developed anisotropic magnetic turbulence in a laboratory plasma. The turbulence has broad (narrow) spectral power in the perpendicular (parallel) direction to the local mean magnetic field extending beyond the ion cyclotron frequency. Its k[see symbol] spectrum is asymmetric in the ion and electron diamagnetic directions. The wave number scaling for the short wavelength fluctuations shows exponential falloff indicative of dissipation. A standing wave structure is found for the turbulence in the minor radial direction of the toroidal plasma.

Magnetic-fluctuation-induced particle transport and density relaxation in a high-temperature plasma.

The first direct measurement of magnetic-fluctuation-induced particle flux in the core of a high-temperature plasma is reported. Transport occurs due to magnetic field fluctuations associated with global tearing instabilities. The electron particle flux, resulting from the correlated product of electron density and radial magnetic fluctuations, accounts for density profile relaxation during a magnetic reconnection event. The measured particle transport is much larger than that expected for ambipolar particle diffusion in a stochastic magnetic field.

Fungi and pollen exposure in the first months of life and risk of early childhood wheezing.

BACKGROUND: Many studies have found that the risk of childhood asthma varies by month of birth, but few have examined ambient aeroallergens as an explanatory factor. A study was undertaken to examine whether birth during seasons of elevated ambient fungal spore or pollen concentrations is associated with risk of early wheezing or blood levels of Th1 and Th2 type cells at 24 months of age. METHODS: 514 children were enrolled before birth and followed to 24 months of age. Early wheezing was determined from medical records, and Th1 and Th2 type cells were measured in peripheral blood using flow cytometry. Ambient aeroallergen concentrations were measured throughout the study period and discrete seasons of high spore and pollen concentrations were defined. RESULTS: A seasonal pattern was observed, with birth in autumn to winter (the spore season) associated with increased odds of early wheezing (adjusted odds ratio 3.1; 95% confidence interval 1.3 to 7.4). Increasing mean daily concentrations of basidiospores and ascospores in the first 3 months of life were associated with increased odds of wheeze, as were increasing mean daily concentrations of total and specific pollen types. Levels of Th1 cells at age 24 months were positively associated with mean spore concentrations and negatively associated with mean pollen concentrations in the first 3 months of life. CONCLUSIONS: Children with higher exposure to spores and pollen in the first 3 months of life are at increased risk of early wheezing. This association is independent of other seasonal factors including ambient levels of particulate matter of aerodynamic diameter

Nonambipolar magnetic-fluctuation-induced particle transport and plasma flow in the MST reversed-field pinch.

First direct measurements of nonambipolar magnetic fluctuation-induced charge transport in the interior of a high-temperature plasma are reported. Global resistive tearing modes drive the charge transport which is measured in the vicinity of the resonant surface for the dominant core resonant mode. Finite charge transport has two important consequences. First, it generates a potential well along with locally strong electric field and electric field shear at the resonant surface. Second, this electric field induces a spontaneous E x B driven zonal flow.

Momentum transport from current-driven reconnection in the reversed field pinch.

We calculate momentum transport from tearing fluctuations in a reversed field pinch with sheared flow, considering both the effect of a single tearing mode (through quasilinear theory and MHD computation) and multiple tearing modes (through nonlinear MHD computation). A single tearing mode transports momentum, via Maxwell and Reynolds stresses, more rapidly than classical viscous forces. Moreover, the transport is enhanced by nonlinear coupling of multiple modes.

Spatially resolved measurements of ion heating during impulsive reconnection in the Madison Symmetric Torus.

The impurity ion temperature evolution has been measured during three types of impulsive reconnection events in the Madison Symmetric Torus reversed field pinch. During an edge reconnection event, the drop in stored magnetic energy is small and ion heating is observed to be limited to the outer half of the plasma. Conversely, during a global reconnection event the drop in stored magnetic energy is large, and significant heating is observed at all radii. For both kinds of events, the drop in magnetic energy is sufficient to explain the increase in ion thermal energy. However, not all types of reconnection lead to ion heating. During a core reconnection event, both the stored magnetic energy and impurity ion temperature remain constant. The results suggest that a drop in magnetic energy is required for ions to be heated during reconnection, and that when this occurs heating is localized near the reconnection layer.

Cause of sudden magnetic reconnection in a laboratory plasma.

The cause for sudden reconnection in reversed field pinch plasmas is determined experimentally for two cases: large reconnection events (the sawtooth crash) and small reconnection events during improved confinement. We measure the term in the MHD equations which represents the driving (or damping) of edge tearing modes due to the axisymmetric magnetic field. The term is negative for large reconnection events (the modes are stable, implying that reconnection may be driven by nonlinear coupling to other modes) and positive for small reconnection events (modes are unstable, reconnection is spontaneous).