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Mol Cell Endocrinol ; 53(3): 221-6, 1987 Oct.
Article in English | MEDLINE | ID: mdl-2822514

ABSTRACT

The action of luteinizing hormone on topoisomerase I activity from rat Leydig cells was studied. Stimulation of the enzyme was observed after long-term (24 and 48 h) gonadotrophin treatment in in vivo experiments. No change could be detected for shorter times than 12 h using two different experimental approaches. Topoisomerase I was stimulated by cAMP in a whole cell extract in a phosphorylation-dependent manner. These results suggest that topoisomerase I could be a target for nuclear events induced by peptide hormone action.


Subject(s)
Cyclic AMP/physiology , DNA Topoisomerases, Type I/metabolism , Intracellular Signaling Peptides and Proteins , Leydig Cells/enzymology , Luteinizing Hormone/physiology , Animals , Carrier Proteins/pharmacology , Gonadotropins/pharmacology , In Vitro Techniques , Leydig Cells/drug effects , Male , Rats , Rats, Inbred Strains , Testosterone/blood
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