Label-free highly multimodal nonlinear endoscope.

We demonstrate a 2 mm diameter highly multimodal nonlinear micro-endoscope allowing label-free imaging of biological tissues. The endoscope performs multiphoton fluorescence (3-photon, 2-photon), harmonic generation (second-SHG and third-THG) and coherent anti-Stokes Raman scattering (CARS) imaging over a field of view of 200 µm. The micro-endoscope is based on a double-clad antiresonant hollow core fiber featuring a high transmission window (850 nm to 1800 nm) that is functionalized with a short piece of graded-index (GRIN) fiber. When combined with a GRIN micro-objective, the micro-endoscope achieves a 1.1 µm point spread function (PSF). We demonstrate 3-photon, 2-photon, THG, SHG, and CARS high resolution images of unlabelled biological tissues.

Double clad tubular anti-resonant hollow core fiber for nonlinear microendoscopy.

We report the fabrication and characterization of the first double clad tubular anti-resonant hollow core fiber. It allows to deliver ultrashort pulses without temporal nor spectral distortions in the 700-1000 nm wavelength range and to efficiently collect scattered light in a high numerical aperture double clad. The output fiber mode is shaped with a silica microsphere generating a photonic nanojet, making it well suitable for nonlinear microendoscopy application. Additionally, we provide an open access software allowing to find optimal drawing parameters for the fabrication of tubular hollow core fibers.

Image-based adaptive optics for in vivo imaging in the hippocampus.

Adaptive optics is a promising technique for the improvement of microscopy in tissues. A large palette of indirect and direct wavefront sensing methods has been proposed for in vivo imaging in experimental animal models. Application of most of these methods to complex samples suffers from either intrinsic and/or practical difficulties. Here we show a theoretically optimized wavefront correction method for inhomogeneously labeled biological samples. We demonstrate its performance at a depth of 200 µm in brain tissue within a sparsely labeled region such as the pyramidal cell layer of the hippocampus, with cells expressing GCamP6. This method is designed to be sample-independent thanks to an automatic axial locking on objects of interest through the use of an image-based metric that we designed. Using this method, we show an increase of in vivo imaging quality in the hippocampus.

In vivo single human sweat gland activity monitoring using coherent anti-Stokes Raman scattering and two-photon excited autofluorescence microscopy.

BACKGROUND: Eccrine sweat secretion is of central importance for control of body temperature. Although the incidence of sweat gland dysfunction might appear of minor importance, it can be a real concern for people with either hypohidrosis or hyperhidrosis. However, sweat gland function remains relatively poorly explored. OBJECTIVES: To investigate the function of single human sweat glands. METHODS: We describe a new approach for noninvasive imaging of single sweat gland activity in human palms in vivo up to a depth of 100 µm, based on nonlinear two-photon excited autofluorescence (TPEF) and coherent anti-Stokes Raman scattering (CARS). RESULTS: These techniques appear to be useful compared with approaches already described for imaging single sweat gland activity, as they allow better three-dimensional spatial resolution of sweat pore inner morphology and real-time monitoring of individual sweat events. By filling the sweat pore with oil and tuning the CARS contrast at 2845 cm(-1) , we imaged the ejection of sweat droplets from a single sweat gland when oil is pushed out by sweat flow. On average, sweat events lasted for about 30 s every 3 min under the conditions studied. On the other hand, about 20% of sweat glands were found inactive. TPEF and CARS were also used to study, at the single pore level, the antiperspirant action of aluminium chlorohydrate (ACH) and to reveal, for the first time in vivo, the formation of a plug at the pore entrance, in agreement with reported ACH antiperspirant mechanisms. CONCLUSIONS: Although data were acquired on human palms, these techniques show great promise for a better understanding of sweat secretion physiology and should be helpful to improve the efficacy of antiperspirant formulations.

All-fiber spectral compression of picosecond pulses at telecommunication wavelength enhanced by amplitude shaping.

We demonstrate efficient spectral compression of picosecond pulses in an all-fiber configuration at telecommunication wavelengths. A spectral compression by a factor of 12 is achieved. Performing temporal shaping with a parabolic pulse significantly improves the spectral compression with much lower substructures and an enhanced Strehl ratio.

Coherent anti-Stokes Raman scattering (CARS) microscopy imaging at interfaces: evidence of interference effects.

We show in this paper that the contrast of the interface between resonant and nonresonant media imaged in Coherent anti-Stokes Raman scattering (CARS) microscopy strongly depends on the pump and Stokes fields spectral detuning. More specifically, when this detuning drives the vibrational resonance with the maximum phase difference, a spatial dip appears at the interface in the CARS image. This effect is studied both theoretically and experimentally and is an evidence of the coherent and resonant nature of the CARS contrast mechanism.

Specific docking of apolipoprotein A-I at the cell surface requires a functional ABCA1 transporter.

The identification of defects in ABCA1 as the molecular basis of Tangier disease has highlighted its crucial role in the loading with phospholipids and cholesterol of nascent apolipoprotein particles. Indeed the expression of ABCA1 affects apolipoprotein A-I (apoA-I)-mediated removal of lipids from cell membranes, and the possible role of ABCA1 as an apoA-I surface receptor has been recently suggested. In the present study, we have investigated the role of the ABCA1 transporter as an apoA-I receptor with the analysis of a panel of transfectants expressing functional or mutant forms of ABCA1. We provide experimental evidence that the forced expression of a functional ABCA1 transporter confers surface competence for apoA-I binding. This, however, appears to be dependent on ABCA1 function. Structurally intact but ATPase-deficient forms of the transporter fail to elicit a specific cell association of the ligand. In addition the diffusion parameters of membrane-associated apoA-I indicate an interaction with membrane lipids rather than proteins. These results do not support a direct molecular interaction between ABCA1 and apoA-I, but rather suggest that the ABCA1-induced modification of the lipid distribution in the membrane, evidenced by the phosphatidylserine exofacial flopping, generates a biophysical microenvironment required for the docking of apoA-I at the cell surface.

Far-field radiation from quantum boxes located in pillar microcavities.

Far-field radiation for quantum boxes located in pillar microcavities was investigated spatially and spectrally at room temperature. We have found that small-diameter pillars show directional emission for the fundamental cavity mode together with a spectral behavior dominated by the pillar's discrete modal structure. These results may be important in the context of single-photon emitters for quantum communications.

Fluorescence imaging of submicrometric lattices of colour centres in LiF by an apertureless scanning near-field optical microscope.

We report fluorescence imaging of colour centres in Lithium Fluoride (LiF) using an apertureless Scanning Near Field Optical Microscope (SNOM). The sample consists of periodically spaced submicrometric coloured areas F2 laser-active colour centres produced by low-energy electron beam lithography on the surface of a LiF thin film. A silicon Atomic Force Microscope (AFM) tip is used as an apertureless optical probe. AFM images show a uniform surface roughness with a RMS of 7.2 nm. The SNOM images of the red fluorescence of colour centres excited at lambda = 458 nm with an argon ion laser show that the local photon emission is unambiguously related to the coloured areas and that topographic artefacts can be excluded.

Dipole radiation into grating structures

We present a detailed electromagnetic analysis for the radiation of an electric source located inside grating structures. Our analysis is based on the differential method and uses the scattering-matrix algorithm. We show that gratings that exhibit periodic modulations along two spatial directions (crossed gratings) enable one to couple out the totality of the light emitted by the source into the guided modes of the structure. This property is investigated through the computation of the far-field radiation patterns for crossed gratings with various etching depths. One key result is the possibility to confine the emitted light in a direction about the sample normal, a property that is of interest in the context of spontaneous emission control by microcavity structures.

Extraction of light from sources located inside waveguide grating structures.

A crossed waveguide grating is presented that can extract the total guided-mode power emitted by a pointsource dipole located in the structure. Results obtained with rigorous numerical simulations are compared with a simple graphic analysis to facilitate an understanding of the far-field radiation pattern of such a luminescent device.

Light emission from sources located within metallodielectric planar microcavities.

A simple, rigorous electromagnetic formula is derived for predicting the electromagnetic power provided by sources located in transparent or dissipative planar microcavities. With this simple approach, we compare numerically and experimentally the electromagnetic power that escapes the microcavity when the source is located in a metallodielectric or in an all-dielectric resonant planar structure. Although a strong light-extraction coefficient might be expected for metallodielectric microcavities, we show that these attractive structures suffer from metal absorption even when thin metallic layers are used. Experiments implemented with europium chelates located in metallodielectric or in all-dielectric microcavities confirm this result.

Thin-film luminescence under ultraviolet irradiation.

The features of visible dielectric thin-film luminescence under UV irradiation are discussed for single layers with a particular high-index/low-index couple, HfO(2)/SiO(2). We exploit those results in an attempt to understand the proper luminescence of three different mirror stacks in terms of both luminescence efficiency and angular emission.

Fluorescence of Ta(2)O(5) thin films doped by kilo-electron-volt Er implantation: application to microcavities.

Luminescent layers are prepared by the implantation of kilo-electron-volt Er ions into tantalum pentoxide (Ta(2)O(5)) thin films made by ion plating. The implantation fluences range from 3.3 × 10(14) to 2 × 10(15) ions/cm(2), and the energies range from 190 to 380 keV. Refractive index, extinction coefficient, and losses on guided propagation are investigated. We show that these Er-implanted layers present an absorption as low as that of the nonimplanted films. When optically pumped with an Ar(+) laser (λ = 0.488 µm) beam, implanted films show peaked fluorescence spectra centered near 1.53 and 0.532 µm. We show that the fluorescence intensity is correlated with the intensity of the pump beam in the region where Er ions are implanted. Radiation patterns of Er ions located inside a single layer or inside a Ta(2)O(5)/SiO(2) dielectric stack made by ion plating are also investigated. We show that, in any case, spontaneous emission of Er ions can be spatially controlled.

Light-induced refractive-index modifications in dielectric thin films: experimental determination of relaxation time and amplitude.

A two-beam setup based on the totally reflecting prism coupler is shown to be a powerful means of characterizing light-induced refractive-index modifications in dielectric thin films. Rise and relaxation times and amplitudes of thin-film refractive-index variations can be measured. Some developments of the electromagnetic theory of prism coupling are presented for Gaussian incident beams. Measurements made on a single Ta(2)O(5) layer deposited on a silica glass are presented. Relaxation times of a few milliseconds reveal the thermal origin of the phenomena. The thermal nonlinear coefficient of this Ta(2)O(5) layer is nearly 10(-15) m(2)/W.

Consequences of Ti-, Li-, and Er-ion implantations on the optical properties of single layers of Ta(2)O(5).

Tantalum pentoxide (Ta(2)O(5)) layers made by ion plating are implanted with a high fluence of keV Ti, Li, and Er ions. The resulting refractive-index profiles are given from the analysis of guided-wave propagation conditions. A comparison with spectrophotometric measurements is presented. All the implanted layers present low losses (extinction coefficient of some 10(-6)) after thermal annealing in air. Ti-implanted layers exhibit an increase in refractive index, whereas Li- and Er-implanted layers present a slight decrease in refractive index. Er-implanted layers present photoluminescent properties.

Nonlinear totally reflecting prism coupler: thermomechanic effects and intensity-dependent refractive index of thin films.

Starting with an accurate linear electromagnetic theory of a totally reflecting prism coupled to a dielectric waveguide, we implement a numerical technique to take into account optogeometric perturbations in stratified media. We calculate both the reflected fields in intensity on the prism base (near field) and in infinity (far field) for an incident Gaussian beam. The study of the variations of the intensity in the reflected beam (near and far fields) versus light power shows thermoinduced dilation of the prism and an intensity-dependent refractive index of thin films composed of tantalium pentoxyde and titanium dioxide.

Characterization by guided wave of instabilities of optical coatings submitted to high-power flux: thermal and third-order nonlinear properties of dielectric thin films.

We study the reversible refractive index variations of optical thin films submitted to a high-power light flux. As a preliminary step, we study the thermorefractive coefficient ?n/?T and the laser damage threshold of our materials. From the hypothesis of a localized optical Kerr-type effect, we use the m-line technique to estimate the nonlinear refractive-index coefficients n(2) of TiO(2), Ta(2)O(5), and ZnS films with continuous illumination. Very large values of n(2) are found for the films obtained by conventional evaporation condensation. On the other hand, the study of the change in the decoupling direction of a low-power light guided in a layer disturbed by a pulsed YAG laser gives a first analysis of the phenomena versus time.