ABSTRACT
The novel HLA-C*01:273 allele, first described in a potential bone marrow donor from Brazil.
Subject(s)
Alleles , Exons , HLA-C Antigens , Humans , HLA-C Antigens/genetics , Brazil , Histocompatibility Testing , Base Sequence , Tissue Donors , Sequence Analysis, DNA/methods , Sequence Alignment , CodonABSTRACT
The novel HLA-A*33:01:21 allele, first described in a potential bone marrow donor from Brazil.
Subject(s)
Alleles , Exons , HLA-A Antigens , Humans , Base Sequence , Bone Marrow Transplantation , Brazil , Histocompatibility Testing , HLA-A Antigens/genetics , Sequence Analysis, DNA/methods , Tissue DonorsABSTRACT
The novel HLA-DRB1*03:215 allele, first described in a potential bone marrow donor from Brazil.
Subject(s)
Alleles , HLA-DRB1 Chains , Histocompatibility Testing , Humans , HLA-DRB1 Chains/genetics , Histocompatibility Testing/methods , Exons , Sequence Analysis, DNA/methods , Tissue Donors , Brazil , High-Throughput Nucleotide Sequencing/methodsABSTRACT
The novel HLA-DQB1*03:01:61 allele, first described in a potential bone marrow donor from Brazil.
Subject(s)
Alleles , Exons , HLA-DQ beta-Chains , Histocompatibility Testing , Humans , Base Sequence , Brazil , Codon , HLA-DQ beta-Chains/genetics , Sequence Analysis, DNA , Tissue DonorsABSTRACT
The novel HLA-B*18:243 allele, first described in a potential bone marrow donor from Brazil.
Subject(s)
HLA-B18 Antigen , Humans , Alleles , Brazil , Exons , High-Throughput Nucleotide Sequencing/methods , Histocompatibility Testing/methods , HLA-B18 Antigen/genetics , HLA-B18 Antigen/immunology , Sequence Analysis, DNA/methods , Tissue DonorsABSTRACT
The novel HLA-A*02:1140 allele, first described in a potential bone marrow donor from Brazil.
Subject(s)
Alleles , HLA-A2 Antigen , Humans , Brazil , HLA-A2 Antigen/genetics , HLA-A2 Antigen/immunology , Histocompatibility Testing , Exons , Tissue Donors , Base Sequence , Sequence Analysis, DNA/methods , Sequence AlignmentABSTRACT
The novel HLA-C*15:274 allele, first described in a potential bone marrow donor from Brazil.
Subject(s)
Alleles , Exons , HLA-C Antigens , Histocompatibility Testing , Humans , HLA-C Antigens/genetics , Histocompatibility Testing/methods , Sequence Analysis, DNA/methods , Tissue Donors , Brazil , High-Throughput Nucleotide Sequencing/methods , Base SequenceABSTRACT
The novel HLA-B*35:593 allele, first described in a potential bone marrow donor from Brazil.
Subject(s)
Exons , HLA-B Antigens , Humans , Alleles , Base Sequence , Brazil , High-Throughput Nucleotide Sequencing/methods , Histocompatibility Testing/methods , HLA-B Antigens/genetics , HLA-B35 Antigen/genetics , Sequence Analysis, DNA/methods , Tissue DonorsABSTRACT
The novel HLA-C*08:275 allele, first described in a potential bone marrow donor from Brazil.
Subject(s)
Alleles , Exons , HLA-C Antigens , Humans , HLA-C Antigens/genetics , Brazil , Histocompatibility Testing , Tissue Donors , Base Sequence , Sequence Analysis, DNA/methods , Sequence Alignment , CodonABSTRACT
The novel HLA-A*30:218 allele, first described in a potential bone marrow donor from Brazil.
Subject(s)
Alleles , Exons , HLA-A Antigens , Histocompatibility Testing , Sequence Analysis, DNA , Humans , HLA-A Antigens/genetics , Sequence Analysis, DNA/methods , Histocompatibility Testing/methods , Tissue Donors , Base Sequence , Brazil , Sequence AlignmentABSTRACT
To control immune responses, regulatory CD4+CD25+Foxp3+ T cells (Treg) maintain their wide and diverse repertoire through continuous arrival of recent thymic emigrants (RTE). However, during puberty, the activity of RTE starts to decline as a natural process of thymic involution, introducing consequences, not completely described, to the repertoire. Type 1 diabetes (T1D) patients show quantitative and qualitative impairments on the Treg cells. Our aim was to evaluate peripheral Treg and RTE cell frequencies, in T1D patients from two distinct age groups (young and adults) and verify if HLA phenotypes are concomitant associated. To this, blood samples from Brazilian twenty established T1D patients (12 young and 8 adults) and twenty-one healthy controls (11 young and 10 adults) were analyzed, by flow cytometry, to verify the percentages of CD4, Treg (CD4+CD25+Foxp3+) and the subsets of CD45RA+ (naive) and CD31+(RTE) within then. Furthermore, the HLA typing was also set. We observed that the young established T1D patients feature decreased frequencies in total Treg cells and naive RTE within Treg cells. Significant prevalence of HLA alleles, associated with risk, in T1D patients, was also identified. Performing a multivariate analysis, we confirmed that the cellular changes described offers significant variables that distinct T1D patients from the controls. Our data collectively highlight relevant aspects about homeostasis imbalances in the Treg cells of T1D patients, especially in young, and disease prognosis; that might contribute for future therapeutic strategies involving Treg cells manipulation.
Subject(s)
Diabetes Mellitus, Type 1 , Forkhead Transcription Factors , Interleukin-2 Receptor alpha Subunit , T-Lymphocytes, Regulatory , Thymus Gland , Humans , Diabetes Mellitus, Type 1/immunology , T-Lymphocytes, Regulatory/immunology , T-Lymphocytes, Regulatory/metabolism , Adult , Brazil , Male , Female , Forkhead Transcription Factors/metabolism , Thymus Gland/immunology , Interleukin-2 Receptor alpha Subunit/metabolism , Young Adult , Adolescent , Immunophenotyping , T-Lymphocyte Subsets/immunology , T-Lymphocyte Subsets/metabolism , ChildABSTRACT
Abstract INTRODUCTION Rio de Janeiro has hardly experienced coronavirus disease. METHODS Here, 87,442 reverse transcription polymerase chain reaction (RT-PCR) test results for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were reported among Rio de Janeiro residents (March to September 2020). RESULTS Overall, RT-PCR positivity of 44.6% decreased over time towards 20%. Positivity was greater among males (OR=1.22; 95%CI:1.19-1.26); Black (OR=1.10; 95%CI:1.02-1.19), Brown (OR=1.16; 95%CI:1.10-1.22), and indigenous people (OR=2.11; 95%CI:0.88-5.03) compared to Whites and increased with age; with epidemic spread from the capital to inland regions. CONCLUSIONS SARS-CoV-2 keeps spreading in Rio de Janeiro, and reopening of activities may fuel the epidemic.