Application of surgical removal and setons abscess drainage management in patients with moderate and severe acne inverse at gluteal and perianal area / 中华医学美学美容杂志

Objective:To investigate the application value and effect of surgical removal and setons abscess drainage in moderate and severe acne inverse.Methods:A retrospective case study of 4 patients (3 males and 1 female with an average of onset of 28 years) with acne inverse at gluteal and perianal area in the Qixia Traditioal Chinese Medical Hospital of Shandong province between June 2016 and August 2020, The operative technique was based on the complete excision of the entire diseased skin and subcutaneous fatty tissue, down to the muscular fascia. The cavity was explored using a probe, two setons were placed in each of the fistula tracts, and the wound were covered with absorptive dressing.Results:All the wounds in 4 patients healed primarily and showed no recurrence after surgery, execpt for one patient who did not take supine position in time after operation which resulted in more bleeding in a short time. The other patients recovered well. The patients were followed up for 6 months without affecting the anal skin function, skin lesions and recurrence.Conclusions:The technique of wide surgical excision and setons abscess drainage is an effective method for moderate and severe acne inverse.

[Study on drug-target binding kinetics profiles of flavonoids in Chrysanthemum morifolium and xanthine oxidase].

Based on the target occupancy mathematical model, the binding kinetic process of potential active ingredients of lowering uric acid in Chrysanthemum morifolium with xanthine oxidase(XOD) was evaluated. The potential active ingredients of lowering uric acid in Ch. morifolium were screened by UPLC-Q-Exactivems MS technology, reference substance identification and in vitro enzymatic kinetics experiments. The binding kinetic parameters of xanthine oxidase and potential inhibitor in Ch. morifolium were determined by surface plasma resonance(SPR). The verified mathematical model of the XOD target occupancy evaluated the kinetic binding process of inhibitors and xanthine oxidase in vivo. According to UPLC-Q-Exactive MS and reference substance identification, 39 potential uric acid-lowering active ingredients in Ch. morifolium extracts were identified and the inhibitory activities of 23 compounds were determined. Three potential xanthine oxidase inhibitors were screened, namely genistein, luteolin, and apigenin. whose IC_(50 )were 1.23, 1.47 and 1.59 µmol·L~(-1), respectively. And the binding rate constants(K_(on)) were 1.26×10~6, 5.23×10~5 and 6.36×10~5 mol·L~(-1)·s~(-1), respectively. The dissociation rate constants(K_(off)) were 10.93×10~(-2), 1.59×10~(-2), and 5.3×10~(-2 )s~(-1), respectively. After evaluation by different administration methods, the three selected compounds can perform rapid and sustained inhibition of xanthine oxidase in vivo under combined administration. This study comprehensively evaluated the target occupancy process of three effective components in different ways of administration in vivo by UPLC-MS, concentration-response method, SPR technology and xanthine oxidase target occupancy model, which would provide a new research idea and method for screening active ingredients in traditional Chinese medicine.

Clinical effects of excision and setons drainage management in treatment of early and medium-term perifolliculitis capitis abscedenset suffodiens / 中华医学美学美容杂志

Objective:To investigate the surgical management of perifolliculitis capitis abscedenset (PCAS).Methods:From June 2015 to October 2018, 8 patients with PCAS were treated, aged 13-26 years, with an average age of 23 years. Hypepigmented plaques in size were from 0.5-4.0 cm. The operative technique was based on the complete excision of the entire diseased skin and subcutaneous fatty tissue, the fistula was explored using a probe, the seton was inserted through the remaining tract in a double-strand fashion, this double-strand elastic seton was then tied over itself without excessive tension. The wound was covered with absorptive dressing.Results:All the wounds in 8 patients healed primarily. All patients were followed up for 6 months and showed no recurrence after surgery.Conclusions:Perifolliculitis capitis abscedens et suffodiens scalp is a rare chronic suppurative skin disease, its etiology and pathogenesis are unclear, but related with follicular atresia, bacterial infection, immune factors, sex hormones, smoking and obesity. The treatment is difficult, including antibiotics, vitamin A acid, biological agents, photodynamic, laser and surgery. The disease is stubborn and refractory. The technique of wide surgical excision and loose setons drainage is an effective method for PCAS.

Identification of endophytic bacteria BZJN1 and research on biological control of root rot of Atractylodes macrocephala / 中国中药杂志

In this study， an endophytic bacteria strain BZJN1 was isolated from Atractylodes macrocephala， and identified as Bacillus subtilis by physiological and biochemical tests and molecular identification. Strain BZJN1 could inhibit the growth of mycelia of Ceratobasidium sp. significantly， and the inhibition rate was more than 70%. The mycelium growth deformity with bulge as spherical and partially exhaustible in apex or central with microscopic observation. The inhibitory rates under 3% and 6% concentrations of the cell free fermentation were 22.7% and 38.7% expectively. The field test proved that the control efficacy of treatment of 1×10⁸ cfu·mL⁻¹ is 75.27% and 72.37% after 10 and 20 days. All the treatments of strain BZJN1 was able to promote the growth of A. macrocephala， the treatment of 1×10⁸ cfu·mL⁻¹ could able to increase the yield to 14.1%.

Clinical effects of bronchoscope combined hot and cold ablation technique in benign and malignant tumors in the airway / 中国内镜杂志

Objective To analyze the clinical effects of bronchoscope combined with hot and cold ablation in treatment of benign and malignant tumors in primary tracheal and bronchial. Methods From January 2013 to November 2015, 30 cases of pathological types of primary tracheal / bronchial benign and malignant tumors patients include: 8 cases of hamartoma, 1 case of lipoma, 4 cases of granuloma, 1case of clear cell carcinoma, 7 cases of small cell lung cancer, 9 cases of squamous cell carcinoma, were treated by using carbon dioxide cryotherapy combined hot ablation under bronchoscope. Then investigated the outcomes. Results 30 patients, 22 males, 8 females, suffered from 1 month to 2 years, underwent surgery bronchoscope interventional treatment under local anesthesia combined with intravenous anesthesia, are taking joint cold ablation technique. All patients airway is completely opened, the lifting of the airway obstruction. Including 19 cases of disposable surgical removal of the lesion; 8 cases of lesions due to wide distribution, 2 times surgery; three cases three times surgery, no serious complications, follow-up without recurrence, blood gas analysis and dyspnea index were significantly improved. Conclusion Bronchoscope cold joint ablation techniques in the treatment of primary tracheal, bronchial benign and malignant tumors is effective, simple, minimally invasive and safe.

[Study of molecular genetics of the size of reproductive organs and litter size in the sow].

The mutations of ESR, PRLR and FSHR genes were investigated by SNP; 103 F2 sows were slaughtered and their reproductive organs were determined; the litter size of sow was recorded. The linkage between mutations of genes and size of reproductive organ, litter size was analyzed. The results revealed: these sows that could provide more piglets with bigger reproductive organs usually carried genotype like as genotype BB on the sites of ESR, FSHRB and the genotype AA on the sites of ESRB and PRLR, that is, the genotype of BB on the sites of ESR or FSHRB in sow was not only helpful to promote growth of reproductive organs, but also benefit to improve litter size; The size of productive organs and litter size of the sow with genotype AA on the sites of ESRB or PRLR were significantly higher than those of sow with genotype AB or BB.