ABSTRACT
The current study employs nanoengineering diatom and TiO2 NPs to form diatom-Si-TiO2 nanoengineered structures to fabricate a dye sensitized solar cell (DSSC) (DsTnas-DSSC). This was characterized and spin coated on a Fluorine-doped Tin Oxide (FTO) anode plate. The counter cathode was prepared by spin coating graphene oxide on a FTO glass plate and using Lugol's iodine as an electrolyte. The power density of DsTnas-DSSC was estimated with different natural dyes in comparison to conventional photosensitive ruthenium dye. It was found that the natural dyes extracted from plants and microalgae show significant power efficiencies in DSSC. The percentage efficiency of maximum power densities (PDmax) of DsTnas-DSSC obtained with photosensitive dyes were 9.4% with synthetic ruthenium dye (control) and 7.19% > 4.08% > 0.72% > 0.58% > 0.061% from natural dyes found in Haematococcus pluvialis (astaxanthin) > Syzygium cumini (anthocyanin) > Rosa indica (anthocyanin) > Hibiscus rosa-sinensis (anthocyanin) > Beta vulgaris (betalains), respectively. Among all the natural dyes used, the PDmax for the control ruthenium dye was 6.164 mW m-2 followed by the highest in astaxanthin natural dye from Haematococcus pluvialis (5.872 mW m-2). Overall, the use of natural dye DsTnas-DSSC makes the fuel cell low cost and an alternative to conventional expensive, metal and synthetic dyes.
ABSTRACT
In this study, the microalgae Haematococcus pluvialis were cultivated in wastewater inoculated into low-density polypropylene plastic air pillows (LDPE-PAPs) under a light stress. The cells were irradiated to different light stresses using white LED lights (WLs) as the control, and broad-spectrum lights (BLs) as a test for the period of 32 days. It was observed that the inoculum (70 × 102 mL-1 cells) of H. pluvialis algal cells increased almost 30 and 40 times in WL and BL, respectively, at day 32 coherent to its biomass productivity. Higher lipid concentration of up to 36.85 µg mL-1 was observed in BL irradiated cells compared to 13.215 µg L-1 dry weight of biomass in WL. The chlorophyll 'a' content was 2.6 times greater in BL (3.46 µg mL-1) compared to that in WL (1.32 µg mL-1) with total carotenoids being about 1.5 times greater in BL compared to WL on day 32. The yield of red pigment 'Astaxanthin' was about 27% greater in BL than in WL. The presence, of different carotenoids including astaxanthin was also confirmed by HPLC, whereas fatty acid methyl esters (FAMEs) were confirmed by GC-MS. This study further confirmed that wastewater alongwith with light stress is suitable for the biochemical growth of H. pluvialis with good biomass yield as well as carotenoid accumulation. Additionally there was 46% reduction in chemical oxygen demand (COD) in a far more efficient manner when cultured in recycled LDPE-PAP. Such type of cultivation of H. pluvialis made the overall process economical and suitable for upscaling to produce value-added products such as lipids, pigments, biomass, and biofuel for commercial applications.
ABSTRACT
Microbial electrochemical technologies (METs) are a group of innovative technologies that produce valuables like bioelectricity and biofuels with the simultaneous treatment of wastewater from microorganisms known as electroactive microorganisms. The electroactive microorganisms are capable of transferring electrons to the anode of a MET through various metabolic pathways such as direct (via cytochrome or pili) or indirect (through transporters) transfer. Though this technology is promising, the inferior yield of valuables and the high cost of reactor fabrication are presently impeding the large-scale application of this technology. Therefore, to overcome these major bottlenecks, a lot of research has been dedicated to the application of bacterial signalling, for instance, quorum sensing (QS) and quorum quenching (QQ) mechanisms in METs to improve its efficacy in order to achieve a higher power density and to make it more cost-effective. The QS circuit in bacteria produces auto-inducer signal molecules, which enhances the biofilm-forming ability and regulates the bacterial attachment on the electrode of METs. On the other hand, the QQ circuit can effectively function as an antifouling agent for the membranes used in METs and microbial membrane bioreactors, which is imperative for their stable long-term operation. This state-of-the-art review thus distinctly describes in detail the interaction between the QQ and QS systems in bacteria employed in METs to generate value-added by-products, antifouling strategies, and the recent applications of the signalling mechanisms in METs to improve their yield. Further, the article also throws some light on the recent advancements and the challenges faced while incorporating QS and QQ mechanisms in various types of METs. Thus, this review article will help budding researchers in upscaling METs with the integration of the QS signalling mechanism in METs.
ABSTRACT
Discharge of untreated or partially treated toxic dyes containing wastewater from textile industries into water streams is hazardous for environment. The use of heavy metal(s) rich dyes, which are chemically active in azo and sulfur content(s) has been tremendously increasing in last two decades. Conventional physical and chemical treatment processes help to eliminate the dyes from textile wastewater but generates the secondary pollutants which create an additional environmental problem. Microalgae especially the diatoms are promising candidate for dye remediation from textile wastewater. Nanoporous diatoms frustules doped with nanocomposites increase the wastewater remediation efficiency due to their adsorption properties. On the other hand, microalgae with photosynthetic microbial fuel cell have shown significant results in being efficient, cost effective and suitable for large scale phycoremediation. This integrated system has also capability to enhance lipid and carotenoids biosynthesis in microalgae while simultaneously generating the bioelectricity. The present review highlights the textile industry wastewater treatment by live and dead diatoms as well as microalgae such as Chlorella, Scenedesmus, Desmodesmus sp. etc. This review engrosses applicability of diatoms and microalgae as an alternative way of conventional dye removal techniques with techno-economic aspects.
Subject(s)
Chlorella , Diatoms , Microalgae , Biomass , Coloring Agents/chemistry , Wastewater/chemistryABSTRACT
Microbial fuel cells are biochemical factories which besides recycling wastewater are electricity generators, if their low power density can be scaled up. This also adds up to work on many factors responsible to increase the cost of running a microbial fuel cell. As a result, the first step is to use environment friendly dead organic algae biomass or even living algae cells in a microbial fuel cell, also referred to as microalgal microbial fuel cells. This can be a techno-economic aspect not only for treating textile wastewater but also an economical way of obtaining value added products and bioelectricity from microalgae. Besides treating wastewater, microalgae in its either form plays an essential role in treating dyes present in wastewater which essentially include azo dyes rich in synthetic ions and heavy metals. Microalgae require these metals as part of their metabolism and hence consume them throughout the integration process in a microbial fuel cell. In this review a detail plan is laid to discuss the treatment of industrial effluents (rich in toxic dyes) employing microbial fuel cells. Efforts have been made by researchers to treat dyes using microbial fuel cell alone or in combination with catalysts, nanomaterials and microalgae have also been included. This review therefore discusses impact of microbial fuel cells in treating wastewater rich in textile dyes its limitations and future aspects.
Subject(s)
Bioelectric Energy Sources , Environmental Pollutants , Microalgae , Coloring Agents/metabolism , Environmental Pollutants/metabolism , Microalgae/metabolism , WastewaterABSTRACT
Microalgae have been recognized as one of the most efficient microorganisms to remediate industrial effluents. Among microalgae diatoms are silica shelled unicellular eukaryotes, found in all types of water bodies and flourish very well even in wastewater. They have their silica cell wall made up of nano arrayed pores arranged in a uniform fashion. Therefore, they act as smart nanocontainers to adsorb various trace metals, dyes, polymers, and drugs which are hazardous to human as well to aquatic life. The beautiful nanoarchitecture in diatoms allows them to easily bind to ligands of choice to form a nanocomposite structure with the pollutants which can be a chemical or biological component. Such naturally available diatom nanomaterials are economical and highly sensitive compared to manmade artificial silica nanomaterials to help in facile removal of the toxic pollutants from wastewater. This review is thus focused on employing diatoms to remediate various pollutants such as heavy metals, dyes, hydrocarbons detected in the wastewater. It also includes different microalgae as biosensors for determination of pollutants in effluents and the perspectives for nanotechnological applications in the field of remediating pollutants through microalgae. The review also discusses in length the hurdles and perspectives of employing microalgae in wastewater remediation.
Subject(s)
Biosensing Techniques , Diatoms/growth & development , Metals, Heavy/analysis , Microalgae/growth & development , Nanoparticles , Wastewater/chemistry , Water Pollutants, Chemical/analysisABSTRACT
Haematococcus pluvialis is a green alga that can accumulate high astaxanthin content, a commercially demanding market keto food. Due to its high predicted market value of about 3.4 billion USD in 2027, it is essential to increase its production. Therefore, it is crucial to understand the genetic mechanism and gene expressions profile during astaxanthin synthesis. The effect of poly- and mono-chromatic light of different wavelengths and different intensities have shown to influence the gene expression towards astaxanthin production. This includes transcriptomic gene analysis in H. pluvialis underneath different levels of illumination stress. This review has placed the most recent data on the effects of light on bioastaxanthin production in the context of previous studies, which were more focused on the biochemical and physiological sides. Doing so, it contributes to delineate new ways along the biotechnological process with the aim to increase bioastaxanthin production while decreasing production costs.