Development of Ginkgo (Ginkgo biloba) Nut Starch Films Containing Cinnamon (Cinnamomum zeylanicum) Leaf Essential Oil.

There have been many studies on the development biodegradable films using starch isolated from various food sources as a substitute for synthetic plastic packaging films. In this study, starch was extracted from ginkgo (Ginkgo biloba) nuts, which were mainly discarded and considered an environment hazard. The prepared starch (GBS) was then used for the preparation of antioxidant films by incorporating various amounts of cinnamon (Cinnamomum zeylanicum) essential oil (CZEO), which provides antioxidant activity. The prepared GBS films with CZEO were characterized by measuring physical, optical, and thermal properties, along with antioxidant activity (ABTS, DPPH, and FRAP) measurements. With the increasing amount of CZEO, the flexibility and antioxidant activities of the GBS films increased proportionally, whereas the tensile strength of the films decreased. The added CZEO also increased the water vapor permeability of the GBS films, and the microstructure of the GBS films was homogeneous overall. Therefore, the obtained results indicate that the developed GBS films containing CZEO are applicable as antioxidant food packaging.

Effect of java citronella essential oil addition on the physicochemical properties of Gelidium corneum-chitosan composite films.

A new composite film was developed by combining Gelidium corneum (GC) with chitosan to enhance the physicochemical characteristics of GC film. In addition, to confer new functional property on the GC-chitosan composite film, various amounts (0.5%, 1.0%, and 1.5%) of java citronella essential oil (JCEO) were incorporated into the film. As the concentration of JCEO increased, the extensibility of the GC-chitosan film improved. In addition, the film became more opaque due to decreased light transmission. Especially, ultraviolet light was completely blocked in the composite films containing JCEO. Radical scavenging activities of the films also increased with increasing JCEO content, indicating that the films have antioxidant activity. Therefore, GC-chitosan composite film containing JCEO is applicable in food packaging to preserve food quality by retarding lipid oxidation.

Combined Treatment of High Hydrostatic Pressure and Cationic Surfactant Washing to Inactivate Listeria monocytogenes on Fresh-Cut Broccoli.

This study was conducted to examine the inactivation effect of the combined treatment of high hydrostatic pressure (HHP; 400 MPa for 1, 3, and 5 min) and cationic surfactant washing (0.05% benzethonium chloride, BEC) against Listeria monocytogenes inoculated on fresh-cut broccoli (FCB). Washing with BEC at concentrations exceeding 0.05% resulted in 2.3 logreduction of L. monocytogenes counts on FCB, whereas HHP treatment had approximately 5.5- 5.6 log-reductions regardless of the treatment time. Scanning electron microscopy corroborated microbial enumeration, revealing that the combined treatment was more effective in removing L. monocytogenes from FCB than individual treatment with HHP or BEC. Color and total glucosinolate content were maintained after the combined treatment, although the hardness of the FCB slightly decreased. The results clearly suggest that the combined treatment of HHP and BEC washing has potential value as a new sanitization method to improve the microbial safety of FCB.

Antibacterial activity of the noni fruit extract against Listeria monocytogenes and its applicability as a natural sanitizer for the washing of fresh-cut produce.

This study was conducted to investigate the antibacterial activity of the noni fruit extract (NFE) against Listeria monocytogenes (ATCC, 19111 and 19115) and assess its applicability for the washing of fresh-cut produce. Based on the results of the disc diffusion test, L. monocytogenes (ATCC, 19111 and 19115) was susceptible to the activity of NFE than other pathogens studied. Additionally, results of the time-kill assay indicated that NFE at a concentration of 0.5-0.7% effectively killed L. monocytogenes within 7 h. Furthermore, analysis of the intracellular components such as nucleic acids and proteins released from the bacterial cells and their SEM imaging revealed that NFE could increase the membrane permeability of cells resulting in their death. Compared to their unwashed samples, washing of romaine lettuce, spinach, and kale with 0.5% NFE gave a reduction of 1.47, 2.28, and 3.38 log CFU/g, respectively against L. monocytogenes (ATCC, 19111 and 19115), which is significantly different to that of NaOCl. A significant correlation was observed between the antibacterial effect induced due to NFE washing with the surface roughness of the fresh-cut produce than its surface hydrophobicity. Moreover, washing with NFE was not found to affect the color of the samples. These results indicated that NFE demonstrates good antibacterial activity against L. monocytogenes and can be used as a natural sanitizer to ensure the microbiological safety of fresh-cut produce.

Development of an antioxidative packaging film based on khorasan wheat starch containing moringa leaf extract.

The aim of this study was to develop and characterize the properties of khorasan wheat starch (KWS) films containing moringa leaf extract (MLE) as an antioxidative packaging material. KWS was isolated from khorasan wheat and used as a film base material. Different amounts (0, 0.4, 0.7, and 1.0%, w/v) of MLE were added to the KWS film-forming solution and the film properties were examined. Tensile strength of the KWS films decreased and elongation at break increased with increasing MLE content. In addition, the KWS films containing MLE possessed good antioxidative activities and ultraviolet light blocking ability. In particular, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) and 2,2-diphenyl-1-picrylhydrazyl radical scavenging abilities of the KWS films with 1.0% MLE were 59.45% and 37.89%, respectively. Moreover, KWS films containing 1.0% MLE were biodegradable within 30 days. These findings indicate that the developed KWS films containing MLE can be applied as a biodegradable packaging material with antioxidative activity.

Surfactant type affects the washing effect of cinnamon leaf essential oil emulsion on kale leaves.

With increasing foodborne illness associated with fresh-cut produce, a chemical sanitizer to substitute chlorine is needed in the food industry. This study examined the washing effect of cinnamon leaf essential oil (CL-EO) emulsions on fresh-cut produce according to the ionic properties of surfactants. Washing effect of CL-EO emulsion (CLC) with cetylpyridinium chloride was the highest among all treatments on kale leaves. After CLC treatment, 1.83 and 1.54 log reductions against Listeria monocytogenes and Escherichia coli O157:H7 were achieved, respectively, compared to washing with distilled water, and had about 0.6 log-reductions higher than NaOCl treatment. CL-EO droplets in CLC had a positive ζ-potential (+55 mV) compared to other emulsions. These results indicate that ionic properties of surfactants are very important in the washing effect of CL-EO emulsion. Additionally, CLC treatment did not affect the quality of kale leaves during storage. Therefore, CLC can be an effective chemical sanitizer for washing kale leaves.

Characterization of Ecklonia cava Alginate Films Containing Cinnamon Essential Oils.

In this study, Ecklonia cava alginate (ECA) was used as a base material for biodegradable films. Calcium chloride (CaCl2) was used as a cross-linking agent, and various concentrations (0%, 0.4%, 0.7%, and 1.0%) of cinnamon leaf oil (CLO) or cinnamon bark oil (CBO) were incorporated to prepare active films. The ECA film containing 3% CaCl2 had a tensile strength (TS) of 17.82 MPa and an elongation at break (E) of 10.36%, which were higher than those of the film without CaCl2. As the content of essential oils (EOs) increased, TS decreased and E increased. Addition of CLO or CBO also provided antioxidant and antimicrobial activities to the ECA films. The antioxidant activity of the ECA film with CBO was higher than that of the film containing CLO. In particular, the scavenging activities of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals in the ECA film containing 1% CBO were 50.45% and 99.37%, respectively. In contrast, the antimicrobial activities against Escherichia coli O157:H7, Salmonella Typhimurium, Staphylococcus aureus, and Listeria monocytogenes were superior in the ECA films with CLO. These results suggest that ECA films containing CLO or CBO can be applied as new active packaging materials.

Combined effect of a positively charged cinnamon leaf oil emulsion and organic acid on the inactivation of Listeria monocytogenes inoculated on fresh-cut Treviso leaves.

This study was performed to examine the inhibitory effect of combined treatments with a positively charged cinnamon leaf oil (P-CL) emulsion and various organic acids against Listeria monocytogenes inoculated on fresh-cut Treviso leaves. Combined treatments with a P-CL emulsion and an organic acid exhibited a higher inhibitory effect than treatment with each alone or NaOCl. The highest inhibitory effect was achieved by combined treatment with the P-CL emulsion and lactic acid (LA), which showed a 2.85-log reduction compared to distilled water washing. In addition, the inhibitory effect of the combined treatment was maintained during 6 days of subsequent storage, and showed a 3.24-3.39-log reduction compared to unwashed samples. Treviso leaves treated with the P-CL emulsion and LA also maintained their sensorial properties, including appearance, odor, hardness, freshness, and overall acceptability, during subsequent storage. Therefore, combined treatment with a P-CL emulsion and LA can be used to effectively ensure the microbial safety and organoleptic quality of fresh-cut Treviso leaves.

Antibacterial activities of a cinnamon essential oil with cetylpyridinium chloride emulsion against Escherichia coli O157:H7 and Salmonella Typhimurium in basil leaves.

This study examined the antibacterial activities of two different cinnamon essential oil emulsions against Escherichia coli O157:H7 and Salmonella Typhimurium on basil leaves. Cinnamon oil (0.25%) treatments containing CPC (0.05%) exhibited greater effects on the pathogenic bacteria than cinnamon oil treatment without this emulsifier (p < 0.05). Treatment with cinnamon bark and leaf oil emulsions (CBE and CLE, respectively) reduced the populations of E. coli O157:H7 by 4.10 and 5.10 log CFU/g, and S. Typhimurium by 2.71 and 2.82 log CFU/g, respectively. Scanning electron micrographs showed morphological changes in the two pathogenic bacteria following emulsion treatment. In addition, there was no difference in the color or ascorbic acid content of the basil leaves by the emulsion treatment. These results suggest that CBE or CLE treatment can be an effective way to ensure the microbial safety of minimally processed vegetables and a good alternative to chlorination treatment in the fresh produce industry.

Sea Squirt Shell Protein and Polylactic Acid Laminated Films Containing Cinnamon Bark Essential Oil.

Sea squirt (Halocynthia roretzi) shell protein (SSP) was used as a biodegradable film material and laminated with polylactic acid (PLA) to improve its physical and water barrier properties. Cinnamon bark oil (CBO) was incorporated into the SSP film as a bioactive material. After laminating with PLA, the tensile strength and elongation at break of the SSP film increased from 4.07 to 9.09 MPa and from 8.68 to 138.84%, respectively. In addition, water vapor permeability and water solubility decreased from 5.62 to 0.91 × 10-9 g m/m2 s Pa and from 42.17% to 23.93%, respectively. DSC results of the SSP films indicate that melting point temperature increased 140.05 to 163.52 °C by laminating PLA. The addition of 0.5%, 0.7%, and 1.0% CBO conferred the antimicrobial activity against four pathogenic bacteria to the SSP/PLA-laminated films. The SSP/PLA-laminated films containing CBO also had antioxidant activities. Therefore, the SSP/PLA-laminated films containing CBO are applicable as biodegradable packaging films. PRACTICAL APPLICATION: Sea squirt shell has been discarded after the consumption of sea squirt, and sea squirt shell protein can be a base material for biodegradable films. In this study, sea squirt shell protein and polylactic acid laminated films containing cinnamon bark essential oil were developed. The developed films are promising environmentally-friendly alternatives for active packaging material.